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The map label for this gene is dusA [H]

Identifier: 110679742

GI number: 110679742

Start: 2389279

End: 2390283

Strand: Direct

Name: dusA [H]

Synonym: RD1_2504

Alternate gene names: 110679742

Gene position: 2389279-2390283 (Clockwise)

Preceding gene: 110679741

Following gene: 110679744

Centisome position: 57.81

GC content: 60.4

Gene sequence:

>1005_bases
ATGCAGATAAACCAACACGCTAAATTGTCTATCGCCCCTATGATGGACTGGACTGATCGCCATTGTCGGATGGTGCATCG
TCAGTTGACGCATCAGTCGCTGCTTTACACGGAGATGGTGACCTCTGCGGCTTTGGTACGGGGTGGGGCTTTGCATTTGC
TGGCGTTTTCGCCGGATGAGCATCCGGTCGCGCTTCAGCTTGGCGGGTCTGACCCCAAGGAACTGGCGCAGGCTGCCATG
CTCGGTCAGACGGCGGGGTATCGCGAAATCAATTTGAACGTGGGGTGCCCGTCTGACCGGGTGCAGTCGGGTTGCTTCGG
TGCGGTGCTTATGGAAAACCCGGGACTGGTTGCCGATTGCGTCTCGGCGATGCGCGAGGCTGTTGACATACCGGTCACGG
TGAAGTGTCGCATCGGCGTTGATGATCAAAACCCGGACGAGGTCTTGCCGGAGTTTCTGGCACGCATCGTGGCGGCCGGG
TGTGAACGCGTGTCGATCCATGCCCGCAAGGCTTGGTTGCAGGGGCTGAGCCCCAAGGAAAACCGCGATATTCCGCCGCT
GGACTATCCCCTCGTGCTGCGGATGAAGGAATATTTTCCCAATCTTCATATTTCGATCAACGGCGGGATCACATCGCTGG
AACAAGCGGTGGGCTTTCTTGAGGCGGGCCTTGATGGCGTGATGATCGGTCGTGCGGCGTATCACACGCCAACGGATGTC
CTGGGCGCGGTGGACCCGGTGATTTTTGGGACCGGCAAGGTCGTGACCGGCGAAGAAGCCGTGCACGCGATGCTCCCCTA
TATCGAGGCGCATGTCACCGGGGGCGGTCGGTTGGCGCAAATTACGCGCCACATGCTGGGCTTGTTTGCGAATCGCCCCG
GTGCGCGGGTCTGGCGGCGCATGTTGTCAGAAGGTGCGCATTTGCCAAATGCCGGGCCAGCGCTGGTGTTGGACGCCCTG
GCGCAGGTTAGGCACCATCAGGAGCTGGCGCAGCAGAGCGGGTAA

Upstream 100 bases:

>100_bases
TGTGTGCAATGTGTCGTCGTAGGAGGGGCGCATTCAGGGTTATCGAGGCGTATTCCAGCGTAGCAGCATTCGCCGCCAAC
AGTTTGAAGATACGGAAAAA

Downstream 100 bases:

>100_bases
CACAGGGCGCAGGTTTGCAGCCCTGCGCGCAGGGTTTTTCAAGCGCCGTTGCATGCAAGGTGCAATCGAGATGGAATGGG
TCGGGTCATGAAAGCCGAAT

Product: tRNA-dihydrouridine synthase A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 334; Mature: 334

Protein sequence:

>334_residues
MQINQHAKLSIAPMMDWTDRHCRMVHRQLTHQSLLYTEMVTSAALVRGGALHLLAFSPDEHPVALQLGGSDPKELAQAAM
LGQTAGYREINLNVGCPSDRVQSGCFGAVLMENPGLVADCVSAMREAVDIPVTVKCRIGVDDQNPDEVLPEFLARIVAAG
CERVSIHARKAWLQGLSPKENRDIPPLDYPLVLRMKEYFPNLHISINGGITSLEQAVGFLEAGLDGVMIGRAAYHTPTDV
LGAVDPVIFGTGKVVTGEEAVHAMLPYIEAHVTGGGRLAQITRHMLGLFANRPGARVWRRMLSEGAHLPNAGPALVLDAL
AQVRHHQELAQQSG

Sequences:

>Translated_334_residues
MQINQHAKLSIAPMMDWTDRHCRMVHRQLTHQSLLYTEMVTSAALVRGGALHLLAFSPDEHPVALQLGGSDPKELAQAAM
LGQTAGYREINLNVGCPSDRVQSGCFGAVLMENPGLVADCVSAMREAVDIPVTVKCRIGVDDQNPDEVLPEFLARIVAAG
CERVSIHARKAWLQGLSPKENRDIPPLDYPLVLRMKEYFPNLHISINGGITSLEQAVGFLEAGLDGVMIGRAAYHTPTDV
LGAVDPVIFGTGKVVTGEEAVHAMLPYIEAHVTGGGRLAQITRHMLGLFANRPGARVWRRMLSEGAHLPNAGPALVLDAL
AQVRHHQELAQQSG
>Mature_334_residues
MQINQHAKLSIAPMMDWTDRHCRMVHRQLTHQSLLYTEMVTSAALVRGGALHLLAFSPDEHPVALQLGGSDPKELAQAAM
LGQTAGYREINLNVGCPSDRVQSGCFGAVLMENPGLVADCVSAMREAVDIPVTVKCRIGVDDQNPDEVLPEFLARIVAAG
CERVSIHARKAWLQGLSPKENRDIPPLDYPLVLRMKEYFPNLHISINGGITSLEQAVGFLEAGLDGVMIGRAAYHTPTDV
LGAVDPVIFGTGKVVTGEEAVHAMLPYIEAHVTGGGRLAQITRHMLGLFANRPGARVWRRMLSEGAHLPNAGPALVLDAL
AQVRHHQELAQQSG

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusA subfamily [H]

Homologues:

Organism=Homo sapiens, GI40807366, Length=273, Percent_Identity=25.2747252747253, Blast_Score=69, Evalue=6e-12,
Organism=Escherichia coli, GI145693211, Length=323, Percent_Identity=57.5851393188854, Blast_Score=353, Evalue=1e-98,
Organism=Escherichia coli, GI1789660, Length=244, Percent_Identity=30.327868852459, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI1788462, Length=242, Percent_Identity=27.6859504132231, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI25144369, Length=241, Percent_Identity=26.5560165975104, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24580595, Length=297, Percent_Identity=25.2525252525253, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI19920448, Length=297, Percent_Identity=25.2525252525253, Blast_Score=81, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004653
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: 1.-.-.-

Molecular weight: Translated: 36135; Mature: 36135

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQINQHAKLSIAPMMDWTDRHCRMVHRQLTHQSLLYTEMVTSAALVRGGALHLLAFSPDE
CCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCC
HPVALQLGGSDPKELAQAAMLGQTAGYREINLNVGCPSDRVQSGCFGAVLMENPGLVADC
CCEEEEECCCCHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHCCCEEEEEECCCCHHHHH
VSAMREAVDIPVTVKCRIGVDDQNPDEVLPEFLARIVAAGCERVSIHARKAWLQGLSPKE
HHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCC
NRDIPPLDYPLVLRMKEYFPNLHISINGGITSLEQAVGFLEAGLDGVMIGRAAYHTPTDV
CCCCCCCCCHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHCCCEEEECCHHCCCCHHH
LGAVDPVIFGTGKVVTGEEAVHAMLPYIEAHVTGGGRLAQITRHMLGLFANRPGARVWRR
HHCCCCCEECCCCEEECHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHCCCCHHHHHHH
MLSEGAHLPNAGPALVLDALAQVRHHQELAQQSG
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQINQHAKLSIAPMMDWTDRHCRMVHRQLTHQSLLYTEMVTSAALVRGGALHLLAFSPDE
CCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCC
HPVALQLGGSDPKELAQAAMLGQTAGYREINLNVGCPSDRVQSGCFGAVLMENPGLVADC
CCEEEEECCCCHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHCCCEEEEEECCCCHHHHH
VSAMREAVDIPVTVKCRIGVDDQNPDEVLPEFLARIVAAGCERVSIHARKAWLQGLSPKE
HHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCC
NRDIPPLDYPLVLRMKEYFPNLHISINGGITSLEQAVGFLEAGLDGVMIGRAAYHTPTDV
CCCCCCCCCHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHCCCEEEECCHHCCCCHHH
LGAVDPVIFGTGKVVTGEEAVHAMLPYIEAHVTGGGRLAQITRHMLGLFANRPGARVWRR
HHCCCCCEECCCCEEECHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHCCCCHHHHHHH
MLSEGAHLPNAGPALVLDALAQVRHHQELAQQSG
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]