BacMap

Definition	Roseobacter denitrificans OCh 114, complete genome.
Accession	NC_008209
Length	4,133,097

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The map label for this gene is yusZ [H]

Identifier: 110679741

GI number: 110679741

Start: 2387762

End: 2388646

Strand: Direct

Name: yusZ [H]

Synonym: RD1_2502

Alternate gene names: 110679741

Gene position: 2387762-2388646 (Clockwise)

Preceding gene: 110679740

Following gene: 110679742

Centisome position: 57.77

GC content: 60.0

Gene sequence:

>885_bases
ATGACAGATACAAAAACAATTCTCATTACCGGCGCAAGCCGCGGTATTGGCCATCTTGCATCGCTGGCGCTGGCTGCACA
CGGCCATAGGGTCTATGCCGGAATGCGCGACATTGCAGGAAGCAACAAGGACGCGGCCTCCGCCATGCAGGCACAGGCAC
AGGCGGAGAGGCTCAACCTTGTCCCGCTTGAACTGGACGTTACAAACGCGCAAGCCTGCCAAACAGCCATCGACAAGATC
GAGGCAGATGGCCCTCTTGATGTGCTGGTCAACAACGCCGGGGTGATGCCTGTCGGTCTGACCGAGGCATTCACGATGGA
GCAGGCAAAGGATGTTTTCGACGTGAACGTCTATGGCATCATGCGTTTGACGCGGGCCGCGCTGCCCTGCATGCGGGCCC
GGAAATCCGGTCTGGTGATCAACCTCAGTTCTTCCGCGGGGCGTTTCGGGATGCCCTATTTCGGTATCTACTGCGCCAGC
AAGTGGGCGGTTGAGGCTTACAGCGAAGCGCTCCACCACGAGCTTGAGACCTTTGGGATCACCTGCGTTTTGATCGAGCC
AAGCGGGCATGGCACAGATCTGGTCACGACAGCACCCGCGCCGCAGGACACGGCGCGTCTGGAGGCCTATGGCGATCTTG
CGGCAGGTCGGGAACGCCTTCTGGACATGTTCGGGCATATGTTCGCAACGGACCTCATTGGGACCGATGCCAATAACGTG
GCGACAGCAATCGCGCAGCTGATCGAGATGCCGGGAGCGCGGCCAATGCGCGTGCAAGTGGGGCACGACATGGGAGTAAG
CGCGGTGAATGAGGCTGTCGCGCCCCTTCAGGCAAAACTGATCGAGACGTTGAAACCGGTCTATCGCGGCGCGCGCCAAG
CATGA

Upstream 100 bases:

>100_bases
GGGCCGCGAAGCGATCTTCGACGCCATCGTGCCGACACGGCCATGATGGCGGGAAAACGCGCGTATCCCTTCAATCAAAC
ACCCTTCAACGAGGTCAGAC

Downstream 100 bases:

>100_bases
TATCGGCTTCCTGCGAGGCTTTGACGCGGAGGTTGTTTTGGCTTGGCCGAATGATAAGCAGCTTGCCGATCCGGATGTCC
TGCACCGTAGTGTCCGCGGC

Product: oxidoreductase, putative

Products: (3R)-hydroxyacyl-[acyl-carrier-protein]; NADP; NADPH; Proton; beta-ketoacyl-ACP [C]

Alternate protein names: ORFA [H]

Number of amino acids: Translated: 294; Mature: 293

Protein sequence:

>294_residues
MTDTKTILITGASRGIGHLASLALAAHGHRVYAGMRDIAGSNKDAASAMQAQAQAERLNLVPLELDVTNAQACQTAIDKI
EADGPLDVLVNNAGVMPVGLTEAFTMEQAKDVFDVNVYGIMRLTRAALPCMRARKSGLVINLSSSAGRFGMPYFGIYCAS
KWAVEAYSEALHHELETFGITCVLIEPSGHGTDLVTTAPAPQDTARLEAYGDLAAGRERLLDMFGHMFATDLIGTDANNV
ATAIAQLIEMPGARPMRVQVGHDMGVSAVNEAVAPLQAKLIETLKPVYRGARQA

Sequences:

>Translated_294_residues
MTDTKTILITGASRGIGHLASLALAAHGHRVYAGMRDIAGSNKDAASAMQAQAQAERLNLVPLELDVTNAQACQTAIDKI
EADGPLDVLVNNAGVMPVGLTEAFTMEQAKDVFDVNVYGIMRLTRAALPCMRARKSGLVINLSSSAGRFGMPYFGIYCAS
KWAVEAYSEALHHELETFGITCVLIEPSGHGTDLVTTAPAPQDTARLEAYGDLAAGRERLLDMFGHMFATDLIGTDANNV
ATAIAQLIEMPGARPMRVQVGHDMGVSAVNEAVAPLQAKLIETLKPVYRGARQA
>Mature_293_residues
TDTKTILITGASRGIGHLASLALAAHGHRVYAGMRDIAGSNKDAASAMQAQAQAERLNLVPLELDVTNAQACQTAIDKIE
ADGPLDVLVNNAGVMPVGLTEAFTMEQAKDVFDVNVYGIMRLTRAALPCMRARKSGLVINLSSSAGRFGMPYFGIYCASK
WAVEAYSEALHHELETFGITCVLIEPSGHGTDLVTTAPAPQDTARLEAYGDLAAGRERLLDMFGHMFATDLIGTDANNVA
TAIAQLIEMPGARPMRVQVGHDMGVSAVNEAVAPLQAKLIETLKPVYRGARQA

Specific function: Fatty acid biosynthesis pathway; first reduction step. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI7657478, Length=262, Percent_Identity=32.0610687022901, Blast_Score=108, Evalue=7e-24,
Organism=Homo sapiens, GI223718074, Length=185, Percent_Identity=37.2972972972973, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI19743808, Length=187, Percent_Identity=34.7593582887701, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI22507405, Length=196, Percent_Identity=36.2244897959184, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI150247226, Length=197, Percent_Identity=36.0406091370558, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI44680136, Length=189, Percent_Identity=31.7460317460317, Blast_Score=92, Evalue=5e-19,
Organism=Homo sapiens, GI44680133, Length=189, Percent_Identity=31.7460317460317, Blast_Score=92, Evalue=5e-19,
Organism=Homo sapiens, GI17738292, Length=189, Percent_Identity=31.7460317460317, Blast_Score=92, Evalue=5e-19,
Organism=Homo sapiens, GI40548397, Length=196, Percent_Identity=33.1632653061224, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI214010158, Length=196, Percent_Identity=33.1632653061224, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI214010156, Length=196, Percent_Identity=33.1632653061224, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI22758144, Length=196, Percent_Identity=33.1632653061224, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI20149619, Length=195, Percent_Identity=28.7179487179487, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI4504503, Length=201, Percent_Identity=30.8457711442786, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI142976729, Length=178, Percent_Identity=30.3370786516854, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI210032110, Length=177, Percent_Identity=29.3785310734463, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI157739938, Length=193, Percent_Identity=26.9430051813472, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI7706318, Length=208, Percent_Identity=26.9230769230769, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI50726952, Length=185, Percent_Identity=35.1351351351351, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI4503817, Length=187, Percent_Identity=25.1336898395722, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI109715829, Length=173, Percent_Identity=30.635838150289, Blast_Score=68, Evalue=9e-12,
Organism=Homo sapiens, GI7705855, Length=211, Percent_Identity=25.1184834123223, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI40807363, Length=189, Percent_Identity=29.1005291005291, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI4557649, Length=169, Percent_Identity=28.4023668639053, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI210032112, Length=107, Percent_Identity=35.5140186915888, Blast_Score=66, Evalue=4e-11,
Organism=Escherichia coli, GI1787335, Length=218, Percent_Identity=31.1926605504587, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1786701, Length=232, Percent_Identity=30.6034482758621, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI1790717, Length=191, Percent_Identity=31.9371727748691, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1787820, Length=186, Percent_Identity=31.1827956989247, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1786812, Length=213, Percent_Identity=27.2300469483568, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI2367175, Length=211, Percent_Identity=32.2274881516588, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1787526, Length=219, Percent_Identity=28.7671232876712, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI87082160, Length=205, Percent_Identity=26.3414634146341, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87082100, Length=185, Percent_Identity=30.2702702702703, Blast_Score=65, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI71994604, Length=204, Percent_Identity=31.3725490196078, Blast_Score=87, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI115534660, Length=195, Percent_Identity=29.7435897435897, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI193210355, Length=235, Percent_Identity=28.5106382978723, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17544670, Length=218, Percent_Identity=30.2752293577982, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI32563809, Length=209, Percent_Identity=27.7511961722488, Blast_Score=78, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17507613, Length=209, Percent_Identity=27.7511961722488, Blast_Score=78, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17557780, Length=202, Percent_Identity=27.7227722772277, Blast_Score=77, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI71994600, Length=176, Percent_Identity=30.1136363636364, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17560176, Length=195, Percent_Identity=26.6666666666667, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17562904, Length=222, Percent_Identity=26.5765765765766, Blast_Score=64, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI115534694, Length=191, Percent_Identity=28.7958115183246, Blast_Score=64, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI17558628, Length=190, Percent_Identity=29.4736842105263, Blast_Score=64, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI17508895, Length=189, Percent_Identity=28.042328042328, Blast_Score=64, Evalue=9e-11,
Organism=Saccharomyces cerevisiae, GI6323882, Length=190, Percent_Identity=35.2631578947368, Blast_Score=97, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6322067, Length=206, Percent_Identity=30.0970873786408, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6322861, Length=187, Percent_Identity=30.4812834224599, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI281364892, Length=175, Percent_Identity=37.1428571428571, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21355631, Length=173, Percent_Identity=29.4797687861272, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24651139, Length=231, Percent_Identity=29.4372294372294, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI28571526, Length=197, Percent_Identity=33.502538071066, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24644339, Length=192, Percent_Identity=30.2083333333333, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI281364494, Length=182, Percent_Identity=31.8681318681319, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI21356301, Length=193, Percent_Identity=26.9430051813472, Blast_Score=71, Evalue=7e-13,
Organism=Drosophila melanogaster, GI21357041, Length=188, Percent_Identity=31.3829787234043, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24643142, Length=184, Percent_Identity=29.8913043478261, Blast_Score=69, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24640492, Length=172, Percent_Identity=29.0697674418605, Blast_Score=68, Evalue=8e-12,
Organism=Drosophila melanogaster, GI21358495, Length=202, Percent_Identity=29.7029702970297, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI116007236, Length=200, Percent_Identity=27, Blast_Score=65, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24639444, Length=219, Percent_Identity=30.1369863013699, Blast_Score=65, Evalue=6e-11,
Organism=Drosophila melanogaster, GI23397609, Length=187, Percent_Identity=32.620320855615, Blast_Score=65, Evalue=7e-11,
Organism=Drosophila melanogaster, GI24641232, Length=221, Percent_Identity=30.316742081448, Blast_Score=64, Evalue=9e-11,
Organism=Drosophila melanogaster, GI116007238, Length=181, Percent_Identity=27.0718232044199, Blast_Score=64, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.100 [C]

Molecular weight: Translated: 31220; Mature: 31089

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDTKTILITGASRGIGHLASLALAAHGHRVYAGMRDIAGSNKDAASAMQAQAQAERLNL
CCCCCEEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCE
VPLELDVTNAQACQTAIDKIEADGPLDVLVNNAGVMPVGLTEAFTMEQAKDVFDVNVYGI
EEEEEECCCHHHHHHHHHHHCCCCCEEEEECCCCCCEECCHHHHHHHHHHHHHCCHHHHH
MRLTRAALPCMRARKSGLVINLSSSAGRFGMPYFGIYCASKWAVEAYSEALHHELETFGI
HHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCE
TCVLIEPSGHGTDLVTTAPAPQDTARLEAYGDLAAGRERLLDMFGHMFATDLIGTDANNV
EEEEECCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
ATAIAQLIEMPGARPMRVQVGHDMGVSAVNEAVAPLQAKLIETLKPVYRGARQA
HHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TDTKTILITGASRGIGHLASLALAAHGHRVYAGMRDIAGSNKDAASAMQAQAQAERLNL
CCCCEEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCE
VPLELDVTNAQACQTAIDKIEADGPLDVLVNNAGVMPVGLTEAFTMEQAKDVFDVNVYGI
EEEEEECCCHHHHHHHHHHHCCCCCEEEEECCCCCCEECCHHHHHHHHHHHHHCCHHHHH
MRLTRAALPCMRARKSGLVINLSSSAGRFGMPYFGIYCASKWAVEAYSEALHHELETFGI
HHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCE
TCVLIEPSGHGTDLVTTAPAPQDTARLEAYGDLAAGRERLLDMFGHMFATDLIGTDANNV
EEEEECCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
ATAIAQLIEMPGARPMRVQVGHDMGVSAVNEAVAPLQAKLIETLKPVYRGARQA
HHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Oxoacyl-[acyl-carrier-protein]; NADPH; D-3-hydroxy-acyl-ACP; NADP [C]

Specific reaction: Oxoacyl-[acyl-carrier-protein] + NADPH = (3R)-hydroxyacyl-[acyl-carrier-protein] + NADP+ D-3-hydroxy-acyl-ACP + NADP = NADPH + Proton + beta-ketoacyl-ACP [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353931; 9384377; 8396117 [H]