Click here to switch to the map view.

The map label for this gene is yiaO [H]

Identifier: 110679574

GI number: 110679574

Start: 2210372

End: 2211430

Strand: Direct

Name: yiaO [H]

Synonym: RD1_2315

Alternate gene names: 110679574

Gene position: 2210372-2211430 (Clockwise)

Preceding gene: 110679573

Following gene: 110679578

Centisome position: 53.48

GC content: 58.73

Gene sequence:

>1059_bases
ATGATAAAACAAACCCTGAAATGCGCGCTCCTTGCGGCCTTGCTCGCCGGGACGGCGATTGCGGCACAGGCGCAGGAATA
CACCATCCGCGCCACCGCCAATTCCAACGAAAACGACGAAGACTACGACGGTCTCGTCGTGTTCAAGAACTACGTGGAAT
CCGCGTCAAACGGCACCATCGCGGTTGAACTGTTCATCGGCACGCAGCTTTGCAGCAACGGTGCCGAATGTCTGCAAGGC
GTGGCGGATGGCACGATTGACGTTTACGTTTCCACATCCGGCGGCGCGTCGGGGATTTTCCCTTACGTGCAGGTGCTCGA
TCTGCCTTATCTGATGGCGGATGATCGTATTGCCGAACATGTGCTGTCGGGTGATTTTACACGGAAAATGCGGGCGATGG
CGCTGGAGGATTCAGGCGATATGATCCGCCTGATGACGATTGGAAACACCGGCGGGTGGCGCAACTTTGCCAACACCAAG
CGCCGCATCGCTGAGCCCTCTGATATGGAAGGTCTGAAGATCCGAACAGTGGTTGCGGATTTGCCGCAGGAATTGGTGCG
TGCGCTTGGCGCATCCCCGACGCCGATTCCGTGGCCTGAGCTTTTCACGTCCTTCCAGACCGGCGTGGTTGAAGGGTCCA
AGAACGGCATCACGGATATCATGGGCATGAAATTTCCGGACGCCGGGCTGAAATATGTCACGCTGGATGGGCACGCCTAC
ATGGGCGCGCTGTGGTGGATGAACAACGCGAAATTCACCGGCATGCCAGAGGATATGCGCCGGGTGGTCGTGGATGGGTT
TTACGCGCTGCAGCAGGCGACTTTTGCCTCGCCCAAGCGCAAATCCATCGCGGCCTATGAGGAATTCGTGGCCGATGGCG
GTGATCTATACGTGCCCACGCCTGAACAAAAAGCGGCCTTCAAAGAGGGAGCGGCCCCTGTGTTCGACTGGTTCAAGGCC
AATGTGGGGCGCGGGGATGAGATATTCGACGCGCTGACAGAGGCCGTGGCGCAGGCCGAAGCCGAAGTCGGTGCGGCGCG
TGCCGCCGATCTGAATTGA

Upstream 100 bases:

>100_bases
GTGGTGATTTTCCTGATCACCTACATACCGGCCATTTCCATGACCATCCCGCGACTGACGGGCTTTGCCAACTGACACAT
CATCCAACTGGGAGGAATAA

Downstream 100 bases:

>100_bases
CCACGAAAGGGCGGCCTCCGGGTCGCCCCGATCATCTGAACGGATACATCCACACCTTATAGTCAGCGACGAGAACATGG
GCCTTGTTGATTTCTTCCGC

Product: TRAP-type C4-dicarboxylate transport system protein, putative

Products: NA

Alternate protein names: 2,3-DKG-binding protein; Extracytoplasmic solute receptor protein yiaO [H]

Number of amino acids: Translated: 352; Mature: 352

Protein sequence:

>352_residues
MIKQTLKCALLAALLAGTAIAAQAQEYTIRATANSNENDEDYDGLVVFKNYVESASNGTIAVELFIGTQLCSNGAECLQG
VADGTIDVYVSTSGGASGIFPYVQVLDLPYLMADDRIAEHVLSGDFTRKMRAMALEDSGDMIRLMTIGNTGGWRNFANTK
RRIAEPSDMEGLKIRTVVADLPQELVRALGASPTPIPWPELFTSFQTGVVEGSKNGITDIMGMKFPDAGLKYVTLDGHAY
MGALWWMNNAKFTGMPEDMRRVVVDGFYALQQATFASPKRKSIAAYEEFVADGGDLYVPTPEQKAAFKEGAAPVFDWFKA
NVGRGDEIFDALTEAVAQAEAEVGAARAADLN

Sequences:

>Translated_352_residues
MIKQTLKCALLAALLAGTAIAAQAQEYTIRATANSNENDEDYDGLVVFKNYVESASNGTIAVELFIGTQLCSNGAECLQG
VADGTIDVYVSTSGGASGIFPYVQVLDLPYLMADDRIAEHVLSGDFTRKMRAMALEDSGDMIRLMTIGNTGGWRNFANTK
RRIAEPSDMEGLKIRTVVADLPQELVRALGASPTPIPWPELFTSFQTGVVEGSKNGITDIMGMKFPDAGLKYVTLDGHAY
MGALWWMNNAKFTGMPEDMRRVVVDGFYALQQATFASPKRKSIAAYEEFVADGGDLYVPTPEQKAAFKEGAAPVFDWFKA
NVGRGDEIFDALTEAVAQAEAEVGAARAADLN
>Mature_352_residues
MIKQTLKCALLAALLAGTAIAAQAQEYTIRATANSNENDEDYDGLVVFKNYVESASNGTIAVELFIGTQLCSNGAECLQG
VADGTIDVYVSTSGGASGIFPYVQVLDLPYLMADDRIAEHVLSGDFTRKMRAMALEDSGDMIRLMTIGNTGGWRNFANTK
RRIAEPSDMEGLKIRTVVADLPQELVRALGASPTPIPWPELFTSFQTGVVEGSKNGITDIMGMKFPDAGLKYVTLDGHAY
MGALWWMNNAKFTGMPEDMRRVVVDGFYALQQATFASPKRKSIAAYEEFVADGGDLYVPTPEQKAAFKEGAAPVFDWFKA
NVGRGDEIFDALTEAVAQAEAEVGAARAADLN

Specific function: Part of the tripartite ATP-independent periplasmic (TRAP) transport system yiaMNO involved in the uptake of 2,3- diketo-L-gulonate. This protein specifically binds 2,3-diketo-L- gulonate. Is not able to bind either L-ascorbate or dehydroascorbate [H]

COG id: COG1638

COG function: function code G; TRAP-type C4-dicarboxylate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 7 family [H]

Homologues:

Organism=Escherichia coli, GI1790004, Length=338, Percent_Identity=26.6272189349112, Blast_Score=118, Evalue=6e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018389
- InterPro:   IPR004682 [H]

Pfam domain/function: PF03480 SBP_bac_7 [H]

EC number: NA

Molecular weight: Translated: 37976; Mature: 37976

Theoretical pI: Translated: 4.36; Mature: 4.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKQTLKCALLAALLAGTAIAAQAQEYTIRATANSNENDEDYDGLVVFKNYVESASNGTI
CCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCEEEEHHHHHCCCCCEE
AVELFIGTQLCSNGAECLQGVADGTIDVYVSTSGGASGIFPYVQVLDLPYLMADDRIAEH
EEEEEECHHHHCCHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHCCCHHHCCHHHHHH
VLSGDFTRKMRAMALEDSGDMIRLMTIGNTGGWRNFANTKRRIAEPSDMEGLKIRTVVAD
HHCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHCCCCCCCCCEEEHHHHHH
LPQELVRALGASPTPIPWPELFTSFQTGVVEGSKNGITDIMGMKFPDAGLKYVTLDGHAY
HHHHHHHHHCCCCCCCCCHHHHHHHHHCCEECCCCCCHHHHCCCCCCCCCEEEEECCCEE
MGALWWMNNAKFTGMPEDMRRVVVDGFYALQQATFASPKRKSIAAYEEFVADGGDLYVPT
EEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEECC
PEQKAAFKEGAAPVFDWFKANVGRGDEIFDALTEAVAQAEAEVGAARAADLN
CCHHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MIKQTLKCALLAALLAGTAIAAQAQEYTIRATANSNENDEDYDGLVVFKNYVESASNGTI
CCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCEEEEHHHHHCCCCCEE
AVELFIGTQLCSNGAECLQGVADGTIDVYVSTSGGASGIFPYVQVLDLPYLMADDRIAEH
EEEEEECHHHHCCHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHCCCHHHCCHHHHHH
VLSGDFTRKMRAMALEDSGDMIRLMTIGNTGGWRNFANTKRRIAEPSDMEGLKIRTVVAD
HHCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHCCCCCCCCCEEEHHHHHH
LPQELVRALGASPTPIPWPELFTSFQTGVVEGSKNGITDIMGMKFPDAGLKYVTLDGHAY
HHHHHHHHHCCCCCCCCCHHHHHHHHHCCEECCCCCCHHHHCCCCCCCCCEEEEECCCEE
MGALWWMNNAKFTGMPEDMRRVVVDGFYALQQATFASPKRKSIAAYEEFVADGGDLYVPT
EEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEECC
PEQKAAFKEGAAPVFDWFKANVGRGDEIFDALTEAVAQAEAEVGAARAADLN
CCHHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503 [H]