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The map label for this gene is dctB [H]

Identifier: 110679530

GI number: 110679530

Start: 2164550

End: 2166253

Strand: Direct

Name: dctB [H]

Synonym: RD1_2265

Alternate gene names: 110679530

Gene position: 2164550-2166253 (Clockwise)

Preceding gene: 110679529

Following gene: 110679531

Centisome position: 52.37

GC content: 60.33

Gene sequence:

>1704_bases
ATGGCTGTGGGCATTCTGCTCGCGCTGGCGGTGACGGTCGTTTCTGTCACCAACAAGCTGCTGACCGACCGTTTCACGGA
TACAACCCGCGACCGTGCCGAACTGAGGCTTGCGCTTTATAGCGGGAACCTGCTGTCCGAGCTGCGCCAGAACGCCATCG
TGCCGCAACTGCTGGCGCGCGACCCGGAGTTGATCGGCGCGCTGAACTCGGCTGATTACACCGCATCAACACGGCGGCTG
ATCTCCTTTGTGGAAGAGATCGGCGCGGCATCCTTGATGTTGCTGGATATTGACGGGCGGACGGTTGCGGCGACCGACCG
TAACAGGTTGGGGTCAAACCACCGGTCAGAGGCCTATTTCGTCGATGCGATCCGTGCCAGCGGCACCGTTTTCACCGTGG
CGGAGCGCGAAGAGGGCGGTTACGGGTTTTTCTATTCGCGCAGGGTGCAGTCGGGCTCGGATGTGTCGGGCGTGATCCTT
GTCGAGGTCGATTTGCAAAAATTCGGCCGGGCCTGGGCCGGGATTTCGGATGCGGTGATCGTGACGGACAGCACCGGCAC
AATCATCCTGACCACGGAACCGCGCTGGCGTGGCCGCACCGAAGAAGAAGCGCTGGCCCGTCAAACACCCCAGAACGCCA
TCCAGCGCGCCATTCAGGCCACCGCAGACTGGACCGCCTTGCCGCCCGATGCCTATCTGCAGGGCGAGGCCGTGATGCGT
CTGGAAACGCGGATCCCGTTCCGCGGTTGGCGCATGACCAGCTACACCACCTATGCCTCGGTGCGTGAACGTGTGAATGG
TGTACTCGCGCTTGAGATCATGGGCTTTGCGATTCTGCTCGCGCTGACCTTCTATGCGCTCAGCCGGCGCACGGCCTTGC
GCATGGCGCTTTTCCAGCGGGAATCTGCCGAATTGAGATCGCTCAACGCTGCGTTGCAGCGGGAAATTGCCGAGCGCAAA
CGGGTGCAGGAAACGCTTGCCGTTGCTGAACAGACCCTTGAACAATCAAGTAAACTGGCCGCTCTGGGCGAAATGTCAGC
CGCCGTCAGTCACGAGTTGAATCAACCCCTTGCCGCGATGAAAACATATCTTGCCGGGGCGCGTTTGTTGCTGCGGCGCA
ACCGCCATGAAGAGGCGCTGTCCTCCTTTGGGCGCATTGACGATCTGATTGAGCGGATGGGGGGCATCACGCGCCAATTG
AAGTCTTACGCCCGCAAAGGACAGGAGGCCTTTGCGCCAGTCCCCATGGGCGATGCGCTGGCCTCAAGCCTCGCGATGAT
GGAGCCGCAATTGCGTCAACGGCAGGTGCAGATCACCCGCATCCTGCCCGATGATCCGGTTGAGGTCATGGGCGACCGGC
TGCGCATTGAACAGGTGATGGTGAACCTCTTGCGCAACGCGATTGATGCAACCAAATCAGCCGCTGCACCCAAGGTCGAA
ATCATCCTTTCTGCGGGGGAAACGGCAACACTCACAGTACGTGACAATGGGCCGGGTATTGAAGACCTCGATGCCCTTTT
TGAGCCATTTTACACGACCAAACAGCCAGGGGATGGTGTCGGGCTTGGGCTTGCTATCTCGTCCCAGATCGTGACAGACC
TTGGTGGAAGGCTTACAGCGCGCAATGGCCAGCAGGGCGGCGCGGTTTTTGAAATGCAATTGCCGATCCTAAGCGGCGAA
CCAGCGAAACAAGCGGCGGAGTAG

Upstream 100 bases:

>100_bases
GTGTGACTTGAGCCGATACGCGCCTTTGGCGTAAGCTGCTTTCATGGAAGCCGAGCTTCAATCAAAAGAGCCGAACCCGA
TCAGCTCATGGCGGGTGCGC

Downstream 100 bases:

>100_bases
ACAATGACACAAGCCATGAAAATCGCGATTGTCGATGACGAACAGGACATGCGCCAATCCATCAGCCAGTGGCTGGCCCT
TTCGGGGTATGACACGGAAA

Product: C4-dicarboxylate transport sensor protein dctB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 567; Mature: 566

Protein sequence:

>567_residues
MAVGILLALAVTVVSVTNKLLTDRFTDTTRDRAELRLALYSGNLLSELRQNAIVPQLLARDPELIGALNSADYTASTRRL
ISFVEEIGAASLMLLDIDGRTVAATDRNRLGSNHRSEAYFVDAIRASGTVFTVAEREEGGYGFFYSRRVQSGSDVSGVIL
VEVDLQKFGRAWAGISDAVIVTDSTGTIILTTEPRWRGRTEEEALARQTPQNAIQRAIQATADWTALPPDAYLQGEAVMR
LETRIPFRGWRMTSYTTYASVRERVNGVLALEIMGFAILLALTFYALSRRTALRMALFQRESAELRSLNAALQREIAERK
RVQETLAVAEQTLEQSSKLAALGEMSAAVSHELNQPLAAMKTYLAGARLLLRRNRHEEALSSFGRIDDLIERMGGITRQL
KSYARKGQEAFAPVPMGDALASSLAMMEPQLRQRQVQITRILPDDPVEVMGDRLRIEQVMVNLLRNAIDATKSAAAPKVE
IILSAGETATLTVRDNGPGIEDLDALFEPFYTTKQPGDGVGLGLAISSQIVTDLGGRLTARNGQQGGAVFEMQLPILSGE
PAKQAAE

Sequences:

>Translated_567_residues
MAVGILLALAVTVVSVTNKLLTDRFTDTTRDRAELRLALYSGNLLSELRQNAIVPQLLARDPELIGALNSADYTASTRRL
ISFVEEIGAASLMLLDIDGRTVAATDRNRLGSNHRSEAYFVDAIRASGTVFTVAEREEGGYGFFYSRRVQSGSDVSGVIL
VEVDLQKFGRAWAGISDAVIVTDSTGTIILTTEPRWRGRTEEEALARQTPQNAIQRAIQATADWTALPPDAYLQGEAVMR
LETRIPFRGWRMTSYTTYASVRERVNGVLALEIMGFAILLALTFYALSRRTALRMALFQRESAELRSLNAALQREIAERK
RVQETLAVAEQTLEQSSKLAALGEMSAAVSHELNQPLAAMKTYLAGARLLLRRNRHEEALSSFGRIDDLIERMGGITRQL
KSYARKGQEAFAPVPMGDALASSLAMMEPQLRQRQVQITRILPDDPVEVMGDRLRIEQVMVNLLRNAIDATKSAAAPKVE
IILSAGETATLTVRDNGPGIEDLDALFEPFYTTKQPGDGVGLGLAISSQIVTDLGGRLTARNGQQGGAVFEMQLPILSGE
PAKQAAE
>Mature_566_residues
AVGILLALAVTVVSVTNKLLTDRFTDTTRDRAELRLALYSGNLLSELRQNAIVPQLLARDPELIGALNSADYTASTRRLI
SFVEEIGAASLMLLDIDGRTVAATDRNRLGSNHRSEAYFVDAIRASGTVFTVAEREEGGYGFFYSRRVQSGSDVSGVILV
EVDLQKFGRAWAGISDAVIVTDSTGTIILTTEPRWRGRTEEEALARQTPQNAIQRAIQATADWTALPPDAYLQGEAVMRL
ETRIPFRGWRMTSYTTYASVRERVNGVLALEIMGFAILLALTFYALSRRTALRMALFQRESAELRSLNAALQREIAERKR
VQETLAVAEQTLEQSSKLAALGEMSAAVSHELNQPLAAMKTYLAGARLLLRRNRHEEALSSFGRIDDLIERMGGITRQLK
SYARKGQEAFAPVPMGDALASSLAMMEPQLRQRQVQITRILPDDPVEVMGDRLRIEQVMVNLLRNAIDATKSAAAPKVEI
ILSAGETATLTVRDNGPGIEDLDALFEPFYTTKQPGDGVGLGLAISSQIVTDLGGRLTARNGQQGGAVFEMQLPILSGEP
AKQAAE

Specific function: Member of the two-component regulatory system dctB/dctD involved in the transport of C4-dicarboxylates. DctB functions as a membrane-associated protein kinase that phosphorylates dctD in response to environmental signals [H]

COG id: COG4191

COG function: function code T; Signal transduction histidine kinase regulating C4-dicarboxylate transport system

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=245, Percent_Identity=28.9795918367347, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1790436, Length=229, Percent_Identity=29.6943231441048, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI87081816, Length=270, Percent_Identity=30.3703703703704, Blast_Score=84, Evalue=4e-17,
Organism=Escherichia coli, GI145693157, Length=326, Percent_Identity=25.7668711656442, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1788393, Length=239, Percent_Identity=31.3807531380753, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI48994928, Length=258, Percent_Identity=27.1317829457364, Blast_Score=74, Evalue=4e-14,
Organism=Escherichia coli, GI1786912, Length=271, Percent_Identity=31.3653136531365, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1787894, Length=247, Percent_Identity=27.1255060728745, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790861, Length=220, Percent_Identity=31.8181818181818, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI1790551, Length=260, Percent_Identity=28.0769230769231, Blast_Score=66, Evalue=7e-12,
Organism=Escherichia coli, GI1790346, Length=219, Percent_Identity=27.3972602739726, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR017055
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 62010; Mature: 61879

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVGILLALAVTVVSVTNKLLTDRFTDTTRDRAELRLALYSGNLLSELRQNAIVPQLLAR
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHEEEEEECCHHHHHHHHCCHHHHHHHC
DPELIGALNSADYTASTRRLISFVEEIGAASLMLLDIDGRTVAATDRNRLGSNHRSEAYF
CHHHHCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCEEEECCHHHCCCCCCCCEEE
VDAIRASGTVFTVAEREEGGYGFFYSRRVQSGSDVSGVILVEVDLQKFGRAWAGISDAVI
EEEECCCCCEEEEEECCCCCCCEEEECCCCCCCCCCCEEEEEECHHHHCHHHCCCCCEEE
VTDSTGTIILTTEPRWRGRTEEEALARQTPQNAIQRAIQATADWTALPPDAYLQGEAVMR
EECCCCEEEEECCCCCCCCCHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEE
LETRIPFRGWRMTSYTTYASVRERVNGVLALEIMGFAILLALTFYALSRRTALRMALFQR
EEECCCCCCCEECCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ESAELRSLNAALQREIAERKRVQETLAVAEQTLEQSSKLAALGEMSAAVSHELNQPLAAM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
KTYLAGARLLLRRNRHEEALSSFGRIDDLIERMGGITRQLKSYARKGQEAFAPVPMGDAL
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCHHHH
ASSLAMMEPQLRQRQVQITRILPDDPVEVMGDRLRIEQVMVNLLRNAIDATKSAAAPKVE
HHHHHHHCHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
IILSAGETATLTVRDNGPGIEDLDALFEPFYTTKQPGDGVGLGLAISSQIVTDLGGRLTA
EEEECCCEEEEEEECCCCCHHHHHHHHCCCCCCCCCCCCCEEEHHHHHHHHHHCCCCEEE
RNGQQGGAVFEMQLPILSGEPAKQAAE
CCCCCCCEEEEEECCCCCCCCHHHCCC
>Mature Secondary Structure 
AVGILLALAVTVVSVTNKLLTDRFTDTTRDRAELRLALYSGNLLSELRQNAIVPQLLAR
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHEEEEEECCHHHHHHHHCCHHHHHHHC
DPELIGALNSADYTASTRRLISFVEEIGAASLMLLDIDGRTVAATDRNRLGSNHRSEAYF
CHHHHCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCEEEECCHHHCCCCCCCCEEE
VDAIRASGTVFTVAEREEGGYGFFYSRRVQSGSDVSGVILVEVDLQKFGRAWAGISDAVI
EEEECCCCCEEEEEECCCCCCCEEEECCCCCCCCCCCEEEEEECHHHHCHHHCCCCCEEE
VTDSTGTIILTTEPRWRGRTEEEALARQTPQNAIQRAIQATADWTALPPDAYLQGEAVMR
EECCCCEEEEECCCCCCCCCHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEE
LETRIPFRGWRMTSYTTYASVRERVNGVLALEIMGFAILLALTFYALSRRTALRMALFQR
EEECCCCCCCEECCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ESAELRSLNAALQREIAERKRVQETLAVAEQTLEQSSKLAALGEMSAAVSHELNQPLAAM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
KTYLAGARLLLRRNRHEEALSSFGRIDDLIERMGGITRQLKSYARKGQEAFAPVPMGDAL
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCHHHH
ASSLAMMEPQLRQRQVQITRILPDDPVEVMGDRLRIEQVMVNLLRNAIDATKSAAAPKVE
HHHHHHHCHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
IILSAGETATLTVRDNGPGIEDLDALFEPFYTTKQPGDGVGLGLAISSQIVTDLGGRLTA
EEEECCCEEEEEEECCCCCHHHHHHHHCCCCCCCCCCCCCEEEHHHHHHHHHHCCCCEEE
RNGQQGGAVFEMQLPILSGEPAKQAAE
CCCCCCCEEEEEECCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2134335; 2793824; 11481431; 2551890; 2695394 [H]