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The map label for this gene is rpoD [H]

Identifier: 110679091

GI number: 110679091

Start: 1724150

End: 1726135

Strand: Direct

Name: rpoD [H]

Synonym: RD1_1794

Alternate gene names: 110679091

Gene position: 1724150-1726135 (Clockwise)

Preceding gene: 110679090

Following gene: 110679092

Centisome position: 41.72

GC content: 57.1

Gene sequence:

>1986_bases
ATGGCCGCCAAAGACACCAATGACGACGCAAAGCCAGACGATCAGGATGCGGGCCATTCGCTTGATATGAGTCAGGCGGC
GGTCAAGAAGATGATCGCGGAGGCGCGCGAGAAGGGGTACATCACCTACGATCAGCTCAACACCGTGCTGCCCCCTGATC
AGGTGAATTCCGAACAGATCGAGGACGTGATGTCGATGCTTTCCGAAATGGGCATCAACATCATCGAAGACGAAGACGCC
GAGGAGGAAGAGGCAAAAGGCTCGACCGAGATTGCCACGACCGAAGGCACCCGTGAAGTCACGCTCGGGTCCGGCACCAG
CGAGAAGCTGGACCGCACGGATGATCCGGTTCGCATGTACCTGCGCGAGATGGGGTCGGTTGAACTGCTGAGCCGCGAGG
GCGAAATTGCGATTGCCAAGCGGATCGAAGCTGGCCGGAATACAATGATTTCAGGGCTTTGCGAAAGCCCTTTGACCTTT
CAGGCGATCACGATCTGGCGCGATGAACTCTTGTCTGAGGACATTCTGCTGCGCGATGTGATTGACCTCGAAGCGACCTT
CGGCAATCAACTGGATGAAGACGTCAGCGTCGGAGAGCCGGTGGTGGCTGCGGACGGTGCCACTGCGCCGCAAAAAGAGG
AATCGTCTCCTGAGCTTGACGCGGATGGCAATCCGATTGCCAATGATGACGACGACGATGAGGACGAACAGGCCAACATG
AGCCTCGCCGCGATGGAAGCGGCGTTGAAGCCGCAGGTGCTTGAAGCGCTGGATATCATCGCGAGTGACTACACAAAACT
GAGCGAGATGCAGGACAGCCGCATTTCCGCGACGCTGAATGAAGACGGTAGTTTTGACGAAGGGGATGAAGCGCTCTACC
AAAAGCTGCGCTCTGAAATTGTCCTGCTGGTCAACGAGTTGCACCTGCACAACAACCGTATCGAGGCTTTGATCGACCAG
CTGTACGGGATCAACCGCCGCATCATGCAGATCGATTCCGCGATGGTGAAGCTGGCGGATCAGGCCCGCATCAACCGGCG
TGAATTCGTCGATGAATATCGCGGATACGAGCTTGATCCGAACTGGATGGAGCGGATGACGGAAAAATCCGGGCGTGGAT
GGCAGATGTTCATCGAACGCTCCAGCGACAAGGTCGAGGAACTGCGCGCTGATATGGCACAGGTTGGCCAATACGTGGGT
CTCGATATTTCGGAATTCCGCCGGATCGTGCAGCAGGTGCAAAAGGGCGAGAAGGAAGCGCGTCAGGCCAAGAAGGAAAT
GGTAGAGGCGAACCTGCGTCTGGTTATTTCCATTGCCAAGAAATACACCAACCGCGGCCTGCAATTCCTTGATCTCATTC
AGGAAGGCAACATCGGCCTGATGAAGGCCGTTGACAAGTTTGAATACCGCCGGGGGTATAAATTCTCGACCTATGCCACG
TGGTGGATCCGTCAGGCGATCACACGCTCGATTGCGGATCAGGCGCGGACAATCCGTATTCCTGTGCACATGATCGAGAC
GATCAACAAACTGGTGCGCACGGGCCGCCAGATGCTGCATGAGATCGGGCGTGAACCGACGCCTGAGGAACTGGCCGAGA
AGCTGCAGATGCCGCTTGAGAAAGTGCGCAAGGTGATGAAGATCGCCAAGGAGCCGATTTCGCTTGAGACACCGATTGGC
GATGAGGAAGACAGCCAGCTTGGCGATTTCATCGAGGACAAGAACGCCGTTCTGCCTTTGGACAGCGCCATTCAGGAAAA
CCTCAAGGAAACCACGACCCGCGTGCTGGCGTCCCTTACCCCGCGCGAGGAACGCGTGCTGCGCATGCGCTTTGGTATCG
GTATGAACACGGACCACACGCTCGAAGAGGTCGGCCAGCAGTTCTCGGTCACACGCGAGCGTATCCGCCAGATCGAGGCG
AAGGCGCTGCGCAAACTCAAGCACCCCAGCCGGTCACGCAAGCTGCGGAGTTTCCTCGATCAGTGA

Upstream 100 bases:

>100_bases
GAAATAGTATTGCTTAACGGGTGGTCGAATCACCCAAAACGCTAGATGATTCCCCTGTGATTCGGGCAAGCGAATCAACC
TATTCTGTGAGGAGCAGCGC

Downstream 100 bases:

>100_bases
GCTACGACTTACTGTATTTTTGGCGGACCATTAGATCAAAGTTACCGGATGCGTACGCTGTTATGCACATCGTGCTTGCA
GCAAGTGGTATTGCCTATGT

Product: RNA polymerase sigma factor RpoD

Products: NA

Alternate protein names: Sigma-70 [H]

Number of amino acids: Translated: 661; Mature: 660

Protein sequence:

>661_residues
MAAKDTNDDAKPDDQDAGHSLDMSQAAVKKMIAEAREKGYITYDQLNTVLPPDQVNSEQIEDVMSMLSEMGINIIEDEDA
EEEEAKGSTEIATTEGTREVTLGSGTSEKLDRTDDPVRMYLREMGSVELLSREGEIAIAKRIEAGRNTMISGLCESPLTF
QAITIWRDELLSEDILLRDVIDLEATFGNQLDEDVSVGEPVVAADGATAPQKEESSPELDADGNPIANDDDDDEDEQANM
SLAAMEAALKPQVLEALDIIASDYTKLSEMQDSRISATLNEDGSFDEGDEALYQKLRSEIVLLVNELHLHNNRIEALIDQ
LYGINRRIMQIDSAMVKLADQARINRREFVDEYRGYELDPNWMERMTEKSGRGWQMFIERSSDKVEELRADMAQVGQYVG
LDISEFRRIVQQVQKGEKEARQAKKEMVEANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYAT
WWIRQAITRSIADQARTIRIPVHMIETINKLVRTGRQMLHEIGREPTPEELAEKLQMPLEKVRKVMKIAKEPISLETPIG
DEEDSQLGDFIEDKNAVLPLDSAIQENLKETTTRVLASLTPREERVLRMRFGIGMNTDHTLEEVGQQFSVTRERIRQIEA
KALRKLKHPSRSRKLRSFLDQ

Sequences:

>Translated_661_residues
MAAKDTNDDAKPDDQDAGHSLDMSQAAVKKMIAEAREKGYITYDQLNTVLPPDQVNSEQIEDVMSMLSEMGINIIEDEDA
EEEEAKGSTEIATTEGTREVTLGSGTSEKLDRTDDPVRMYLREMGSVELLSREGEIAIAKRIEAGRNTMISGLCESPLTF
QAITIWRDELLSEDILLRDVIDLEATFGNQLDEDVSVGEPVVAADGATAPQKEESSPELDADGNPIANDDDDDEDEQANM
SLAAMEAALKPQVLEALDIIASDYTKLSEMQDSRISATLNEDGSFDEGDEALYQKLRSEIVLLVNELHLHNNRIEALIDQ
LYGINRRIMQIDSAMVKLADQARINRREFVDEYRGYELDPNWMERMTEKSGRGWQMFIERSSDKVEELRADMAQVGQYVG
LDISEFRRIVQQVQKGEKEARQAKKEMVEANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYAT
WWIRQAITRSIADQARTIRIPVHMIETINKLVRTGRQMLHEIGREPTPEELAEKLQMPLEKVRKVMKIAKEPISLETPIG
DEEDSQLGDFIEDKNAVLPLDSAIQENLKETTTRVLASLTPREERVLRMRFGIGMNTDHTLEEVGQQFSVTRERIRQIEA
KALRKLKHPSRSRKLRSFLDQ
>Mature_660_residues
AAKDTNDDAKPDDQDAGHSLDMSQAAVKKMIAEAREKGYITYDQLNTVLPPDQVNSEQIEDVMSMLSEMGINIIEDEDAE
EEEAKGSTEIATTEGTREVTLGSGTSEKLDRTDDPVRMYLREMGSVELLSREGEIAIAKRIEAGRNTMISGLCESPLTFQ
AITIWRDELLSEDILLRDVIDLEATFGNQLDEDVSVGEPVVAADGATAPQKEESSPELDADGNPIANDDDDDEDEQANMS
LAAMEAALKPQVLEALDIIASDYTKLSEMQDSRISATLNEDGSFDEGDEALYQKLRSEIVLLVNELHLHNNRIEALIDQL
YGINRRIMQIDSAMVKLADQARINRREFVDEYRGYELDPNWMERMTEKSGRGWQMFIERSSDKVEELRADMAQVGQYVGL
DISEFRRIVQQVQKGEKEARQAKKEMVEANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATW
WIRQAITRSIADQARTIRIPVHMIETINKLVRTGRQMLHEIGREPTPEELAEKLQMPLEKVRKVMKIAKEPISLETPIGD
EEDSQLGDFIEDKNAVLPLDSAIQENLKETTTRVLASLTPREERVLRMRFGIGMNTDHTLEEVGQQFSVTRERIRQIEAK
ALRKLKHPSRSRKLRSFLDQ

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=349, Percent_Identity=63.6103151862464, Blast_Score=438, Evalue=1e-124,
Organism=Escherichia coli, GI1789098, Length=225, Percent_Identity=42.6666666666667, Blast_Score=216, Evalue=5e-57,
Organism=Escherichia coli, GI1789871, Length=243, Percent_Identity=27.5720164609054, Blast_Score=86, Evalue=9e-18,
Organism=Escherichia coli, GI1788231, Length=202, Percent_Identity=27.2277227722772, Blast_Score=66, Evalue=6e-12,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR007631
- InterPro:   IPR007127
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04546 Sigma70_ner; PF03979 Sigma70_r1_1; PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 75007; Mature: 74875

Theoretical pI: Translated: 4.43; Mature: 4.43

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAKDTNDDAKPDDQDAGHSLDMSQAAVKKMIAEAREKGYITYDQLNTVLPPDQVNSEQI
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEHHHHCCCCCCCCCCHHHH
EDVMSMLSEMGINIIEDEDAEEEEAKGSTEIATTEGTREVTLGSGTSEKLDRTDDPVRMY
HHHHHHHHHCCCEEECCCCCCHHHCCCCCCEEECCCCCEEEECCCCCHHCCCCCHHHHHH
LREMGSVELLSREGEIAIAKRIEAGRNTMISGLCESPLTFQAITIWRDELLSEDILLRDV
HHHCCCHHHHHCCCCCHHHHHHHHCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
IDLEATFGNQLDEDVSVGEPVVAADGATAPQKEESSPELDADGNPIANDDDDDEDEQANM
HHHHHHHCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
SLAAMEAALKPQVLEALDIIASDYTKLSEMQDSRISATLNEDGSFDEGDEALYQKLRSEI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCHHHHHHHHHHHH
VLLVNELHLHNNRIEALIDQLYGINRRIMQIDSAMVKLADQARINRREFVDEYRGYELDP
HHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
NWMERMTEKSGRGWQMFIERSSDKVEELRADMAQVGQYVGLDISEFRRIVQQVQKGEKEA
HHHHHHHHHCCCCCEEHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
RQAKKEMVEANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYAT
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHH
WWIRQAITRSIADQARTIRIPVHMIETINKLVRTGRQMLHEIGREPTPEELAEKLQMPLE
HHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
KVRKVMKIAKEPISLETPIGDEEDSQLGDFIEDKNAVLPLDSAIQENLKETTTRVLASLT
HHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCC
PREERVLRMRFGIGMNTDHTLEEVGQQFSVTRERIRQIEAKALRKLKHPSRSRKLRSFLD
CHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHC
Q
C
>Mature Secondary Structure 
AAKDTNDDAKPDDQDAGHSLDMSQAAVKKMIAEAREKGYITYDQLNTVLPPDQVNSEQI
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEHHHHCCCCCCCCCCHHHH
EDVMSMLSEMGINIIEDEDAEEEEAKGSTEIATTEGTREVTLGSGTSEKLDRTDDPVRMY
HHHHHHHHHCCCEEECCCCCCHHHCCCCCCEEECCCCCEEEECCCCCHHCCCCCHHHHHH
LREMGSVELLSREGEIAIAKRIEAGRNTMISGLCESPLTFQAITIWRDELLSEDILLRDV
HHHCCCHHHHHCCCCCHHHHHHHHCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
IDLEATFGNQLDEDVSVGEPVVAADGATAPQKEESSPELDADGNPIANDDDDDEDEQANM
HHHHHHHCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
SLAAMEAALKPQVLEALDIIASDYTKLSEMQDSRISATLNEDGSFDEGDEALYQKLRSEI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCHHHHHHHHHHHH
VLLVNELHLHNNRIEALIDQLYGINRRIMQIDSAMVKLADQARINRREFVDEYRGYELDP
HHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
NWMERMTEKSGRGWQMFIERSSDKVEELRADMAQVGQYVGLDISEFRRIVQQVQKGEKEA
HHHHHHHHHCCCCCEEHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
RQAKKEMVEANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYAT
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHH
WWIRQAITRSIADQARTIRIPVHMIETINKLVRTGRQMLHEIGREPTPEELAEKLQMPLE
HHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
KVRKVMKIAKEPISLETPIGDEEDSQLGDFIEDKNAVLPLDSAIQENLKETTTRVLASLT
HHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCC
PREERVLRMRFGIGMNTDHTLEEVGQQFSVTRERIRQIEAKALRKLKHPSRSRKLRSFLD
CHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHC
Q
C

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA