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The map label for this gene is alsS [H]

Identifier: 110678977

GI number: 110678977

Start: 1608866

End: 1610488

Strand: Reverse

Name: alsS [H]

Synonym: RD1_1674

Alternate gene names: 110678977

Gene position: 1610488-1608866 (Counterclockwise)

Preceding gene: 110678978

Following gene: 110678976

Centisome position: 38.97

GC content: 64.08

Gene sequence:

>1623_bases
GTGAGCACCAAAACCCAAATCCGCGCCGCAGACGCGGTTGCGCAAAGATTGTACCGGGCGGGCTGTCGATACGCCTTTGG
CATGCCGGGCGGCGAAGTTCTGACGCTGGTGGATGCGCTTGAGACAGCGGGCATCACCTTTATCCTCGCCAAGCATGAGA
ATGCAGCAGCTTTCATGGCCGAGGGTGTGTATCATCAGACCGGCGCGCCGGGCATTCTGGTGGCGACGGTTGGTCCTGGT
GCCCTGAACGGTGTGAATGCGGTTGCGAATGCACATCAGGACCGCGTGCCGCTGATCGTTCTGGCCGGTGCCGTGGACGC
GGATGAGGCGCAAAGCTACACGCATCAGGTTCTTGACCAGCAGGCCGTCTTTCGACCCATCACCAAAGCCAGCTTTTCGC
TTGTTCCGGGTGCGGCGCATATCATCGCGGACAAAGCCGTGGCCATCGCAACCGAAGGGCGCCCCGGCCCGGTGCATATT
GACGTGCCGATCTCCGTCGCCGACAGCCTTGTGCCCGCAAACGCCCCCCCAGACCGCCAGCCTGCCGCACCAACAGCCCC
CGCCGGCGCTGATCTGGAACAGGCGCGCGCGTGGCTGACCGCTGCCAGACGCCCGATCATCGTCGCGGGCGTTGATGCCA
TGAACCAAGGCTGCGGCGCGACGCTGTTGCAGTTTGCCGAGCGCTTCGGCGCGCCGGTGATCACGACCTATAAGGCCAAA
GGCATCATCCCGGAGGATCACCCGCTGGCCCTTGGCGGTGCGGGTCTGTCGCCGCTGGCGGATAAGCATCTGGTGCCACT
GCTCAATGCCGCTGATCTGATCCTTTGTGTGGGCTATGACCCGATCGAGATGCGCACCGGCTGGCGCGACATCTGGACGC
CGCAAACGCAGAACATCATCGACATAACGGCCGCACCGAATCACCACTACATGCATCAGGCCAGCCTGAACATCATCGCG
GATTGCGCGGCGACGCTTGAGGCGCTGTCCGCAGGGACCACGCCGGCCGCCACTTGGCCCGACCAAGCACCTGCAGCGAC
CAAGGCAGCGCTGGCGGCGGCTTTCCCGAGGGATGATGACTGGGGCCCGGCTGCTGTCATCGACACCTGCCGCACCACCC
TGCCGCGAAAGACCATTGCAACAGCCGACAGTGGCGCGCATCGCATTCTGCTCAGCCAGATGTGGCACTGCCATGCGCCG
CGCGGCCTGATCCAATCCTCTGCCCTTTGCACGATGGGCTGCGCAGTCCCGCTGGCCATGGGCGCGACGCTGGCCACGAC
GGACACGCCGGTGGTGTCGTTTTCCGGGGATGCGGGGTTCCTGATGGTCGCCGGAGAGTTGTCGACTGCCGCCGAACTGG
GGCTGCGCACGATCTTTGTGGTTTTCGTCGATGCCTCCCTCGCCCTGATCGAGCTCAAGCAACGCGCCCGCCAGTTCGGC
AACAACGGCGTGGATTTTGCTGAGCATGATTTCGCGGCCATCGGACGCGCCTTCGGCGGTGCCGGGGTCACAGTCCACAA
CCGCGCAGAGCTACGCGACGCGCTTGAAACAGCACTTTCGGCAGAAACGTTTACCGTGATCGCCGCCAAAATCGAACGGG
GGTCCTATGACGGTCGAATCTGA

Upstream 100 bases:

>100_bases
CAGCGCCACCCTCCTGAAATATCGAAAACGCAAAGGTGAGTTGCGCGTGGCTTCGGGTCAGGTATGAACCGTTGGCAACA
ACGACGGAAAGACAACCAGC

Downstream 100 bases:

>100_bases
AACTGCTGGCGCGCTCGATGGTCTTGTCGTGCTCGACCTCAGCCGCATTCTCGCCGGGCCGACAGCCACGCAGCTGCTCG
GGGATCTGGGCGCGACGGTG

Product: acetolactate synthase, putative

Products: NA

Alternate protein names: ALS; Acetohydroxy-acid synthase [H]

Number of amino acids: Translated: 540; Mature: 539

Protein sequence:

>540_residues
MSTKTQIRAADAVAQRLYRAGCRYAFGMPGGEVLTLVDALETAGITFILAKHENAAAFMAEGVYHQTGAPGILVATVGPG
ALNGVNAVANAHQDRVPLIVLAGAVDADEAQSYTHQVLDQQAVFRPITKASFSLVPGAAHIIADKAVAIATEGRPGPVHI
DVPISVADSLVPANAPPDRQPAAPTAPAGADLEQARAWLTAARRPIIVAGVDAMNQGCGATLLQFAERFGAPVITTYKAK
GIIPEDHPLALGGAGLSPLADKHLVPLLNAADLILCVGYDPIEMRTGWRDIWTPQTQNIIDITAAPNHHYMHQASLNIIA
DCAATLEALSAGTTPAATWPDQAPAATKAALAAAFPRDDDWGPAAVIDTCRTTLPRKTIATADSGAHRILLSQMWHCHAP
RGLIQSSALCTMGCAVPLAMGATLATTDTPVVSFSGDAGFLMVAGELSTAAELGLRTIFVVFVDASLALIELKQRARQFG
NNGVDFAEHDFAAIGRAFGGAGVTVHNRAELRDALETALSAETFTVIAAKIERGSYDGRI

Sequences:

>Translated_540_residues
MSTKTQIRAADAVAQRLYRAGCRYAFGMPGGEVLTLVDALETAGITFILAKHENAAAFMAEGVYHQTGAPGILVATVGPG
ALNGVNAVANAHQDRVPLIVLAGAVDADEAQSYTHQVLDQQAVFRPITKASFSLVPGAAHIIADKAVAIATEGRPGPVHI
DVPISVADSLVPANAPPDRQPAAPTAPAGADLEQARAWLTAARRPIIVAGVDAMNQGCGATLLQFAERFGAPVITTYKAK
GIIPEDHPLALGGAGLSPLADKHLVPLLNAADLILCVGYDPIEMRTGWRDIWTPQTQNIIDITAAPNHHYMHQASLNIIA
DCAATLEALSAGTTPAATWPDQAPAATKAALAAAFPRDDDWGPAAVIDTCRTTLPRKTIATADSGAHRILLSQMWHCHAP
RGLIQSSALCTMGCAVPLAMGATLATTDTPVVSFSGDAGFLMVAGELSTAAELGLRTIFVVFVDASLALIELKQRARQFG
NNGVDFAEHDFAAIGRAFGGAGVTVHNRAELRDALETALSAETFTVIAAKIERGSYDGRI
>Mature_539_residues
STKTQIRAADAVAQRLYRAGCRYAFGMPGGEVLTLVDALETAGITFILAKHENAAAFMAEGVYHQTGAPGILVATVGPGA
LNGVNAVANAHQDRVPLIVLAGAVDADEAQSYTHQVLDQQAVFRPITKASFSLVPGAAHIIADKAVAIATEGRPGPVHID
VPISVADSLVPANAPPDRQPAAPTAPAGADLEQARAWLTAARRPIIVAGVDAMNQGCGATLLQFAERFGAPVITTYKAKG
IIPEDHPLALGGAGLSPLADKHLVPLLNAADLILCVGYDPIEMRTGWRDIWTPQTQNIIDITAAPNHHYMHQASLNIIAD
CAATLEALSAGTTPAATWPDQAPAATKAALAAAFPRDDDWGPAAVIDTCRTTLPRKTIATADSGAHRILLSQMWHCHAPR
GLIQSSALCTMGCAVPLAMGATLATTDTPVVSFSGDAGFLMVAGELSTAAELGLRTIFVVFVDASLALIELKQRARQFGN
NGVDFAEHDFAAIGRAFGGAGVTVHNRAELRDALETALSAETFTVIAAKIERGSYDGRI

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI21361361, Length=580, Percent_Identity=25, Blast_Score=116, Evalue=7e-26,
Organism=Homo sapiens, GI93004078, Length=553, Percent_Identity=22.9656419529837, Blast_Score=115, Evalue=2e-25,
Organism=Escherichia coli, GI87081685, Length=537, Percent_Identity=29.0502793296089, Blast_Score=194, Evalue=8e-51,
Organism=Escherichia coli, GI1790104, Length=543, Percent_Identity=29.097605893186, Blast_Score=194, Evalue=1e-50,
Organism=Escherichia coli, GI1786717, Length=490, Percent_Identity=28.7755102040816, Blast_Score=162, Evalue=4e-41,
Organism=Escherichia coli, GI1788716, Length=550, Percent_Identity=24.3636363636364, Blast_Score=113, Evalue=4e-26,
Organism=Escherichia coli, GI1787096, Length=542, Percent_Identity=24.5387453874539, Blast_Score=109, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17531299, Length=564, Percent_Identity=26.7730496453901, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17531301, Length=564, Percent_Identity=26.7730496453901, Blast_Score=116, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17542570, Length=598, Percent_Identity=22.742474916388, Blast_Score=95, Evalue=8e-20,
Organism=Saccharomyces cerevisiae, GI6323755, Length=568, Percent_Identity=25.7042253521127, Blast_Score=159, Evalue=9e-40,
Organism=Saccharomyces cerevisiae, GI6320816, Length=567, Percent_Identity=22.2222222222222, Blast_Score=96, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6323073, Length=482, Percent_Identity=23.4439834024896, Blast_Score=78, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6323163, Length=482, Percent_Identity=24.4813278008299, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI19922626, Length=478, Percent_Identity=26.7782426778243, Blast_Score=119, Evalue=4e-27,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012782
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 56298; Mature: 56166

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTKTQIRAADAVAQRLYRAGCRYAFGMPGGEVLTLVDALETAGITFILAKHENAAAFMA
CCCCHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHCCEEEEEEECCCCHHHHH
EGVYHQTGAPGILVATVGPGALNGVNAVANAHQDRVPLIVLAGAVDADEAQSYTHQVLDQ
CCCCCCCCCCEEEEEECCCCCCCCHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHH
QAVFRPITKASFSLVPGAAHIIADKAVAIATEGRPGPVHIDVPISVADSLVPANAPPDRQ
HHHHHHHHHCCCEECCCHHHHHHCCEEEEEECCCCCCEEEEECHHHHHHCCCCCCCCCCC
PAAPTAPAGADLEQARAWLTAARRPIIVAGVDAMNQGCGATLLQFAERFGAPVITTYKAK
CCCCCCCCCCCHHHHHHHHHHCCCCEEEEEHHHHCCCCCHHHHHHHHHHCCCEEEEEECC
GIIPEDHPLALGGAGLSPLADKHLVPLLNAADLILCVGYDPIEMRTGWRDIWTPQTQNII
CCCCCCCCEEECCCCCCCCCCCCHHHHCCCCCEEEEECCCCHHHCCCCHHCCCCCCCCEE
DITAAPNHHYMHQASLNIIADCAATLEALSAGTTPAATWPDQAPAATKAALAAAFPRDDD
EEEECCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCCC
WGPAAVIDTCRTTLPRKTIATADSGAHRILLSQMWHCHAPRGLIQSSALCTMGCAVPLAM
CCCHHHHHHHHHHCCCHHEECCCCCHHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHC
GATLATTDTPVVSFSGDAGFLMVAGELSTAAELGLRTIFVVFVDASLALIELKQRARQFG
CCEEEECCCCEEEECCCCCEEEEECCHHHHHHHHHHEEEEEEECCHHHHHHHHHHHHHHC
NNGVDFAEHDFAAIGRAFGGAGVTVHNRAELRDALETALSAETFTVIAAKIERGSYDGRI
CCCCCCHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCC
>Mature Secondary Structure 
STKTQIRAADAVAQRLYRAGCRYAFGMPGGEVLTLVDALETAGITFILAKHENAAAFMA
CCCHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHCCEEEEEEECCCCHHHHH
EGVYHQTGAPGILVATVGPGALNGVNAVANAHQDRVPLIVLAGAVDADEAQSYTHQVLDQ
CCCCCCCCCCEEEEEECCCCCCCCHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHH
QAVFRPITKASFSLVPGAAHIIADKAVAIATEGRPGPVHIDVPISVADSLVPANAPPDRQ
HHHHHHHHHCCCEECCCHHHHHHCCEEEEEECCCCCCEEEEECHHHHHHCCCCCCCCCCC
PAAPTAPAGADLEQARAWLTAARRPIIVAGVDAMNQGCGATLLQFAERFGAPVITTYKAK
CCCCCCCCCCCHHHHHHHHHHCCCCEEEEEHHHHCCCCCHHHHHHHHHHCCCEEEEEECC
GIIPEDHPLALGGAGLSPLADKHLVPLLNAADLILCVGYDPIEMRTGWRDIWTPQTQNII
CCCCCCCCEEECCCCCCCCCCCCHHHHCCCCCEEEEECCCCHHHCCCCHHCCCCCCCCEE
DITAAPNHHYMHQASLNIIADCAATLEALSAGTTPAATWPDQAPAATKAALAAAFPRDDD
EEEECCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCCC
WGPAAVIDTCRTTLPRKTIATADSGAHRILLSQMWHCHAPRGLIQSSALCTMGCAVPLAM
CCCHHHHHHHHHHCCCHHEECCCCCHHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHC
GATLATTDTPVVSFSGDAGFLMVAGELSTAAELGLRTIFVVFVDASLALIELKQRARQFG
CCEEEECCCCEEEECCCCCEEEEECCHHHHHHHHHHEEEEEEECCHHHHHHHHHHHHHHC
NNGVDFAEHDFAAIGRAFGGAGVTVHNRAELRDALETALSAETFTVIAAKIERGSYDGRI
CCCCCCHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7685336; 9353933; 9384377; 10809684 [H]