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The map label for this gene is dut

Identifier: 110678529

GI number: 110678529

Start: 1163631

End: 1164089

Strand: Direct

Name: dut

Synonym: RD1_1201

Alternate gene names: 110678529

Gene position: 1163631-1164089 (Clockwise)

Preceding gene: 110678528

Following gene: 110678530

Centisome position: 28.15

GC content: 62.31

Gene sequence:

>459_bases
ATGGCTTTGGTATCCCTGAAACTGCAATGGGCTGACGGGGCCGACACATCGGTGCCGTTGCCTGCCTATGAGACATCCGG
CGCGGCGGGTGCTGACATCCGCGCAAATTGTCCTGATGGGCCGGTCACACTGGCACCCGGCGCGCGCGCGCTTGTGCCAA
CAGGGCTGCGCATGGCCATTCCGCAGGGCTATGAGGTTCAGATCAGACCCCGGTCGGGCCTCGCGCTCCGGCACGGGATC
ACGCTGGTCAACAGCCCCGGCACCATCGACAGCGATTACCGGGGGGCGGTCGGTGTGATCATGCAGAACCTTGGGGATGC
CGCGTTTGAAATCACCCATGGGATGCGGATCGCGCAAATGGTGGTCGCGCCTGTTGTTCAGGCTAGCTTTGAATTGTCCG
ATAGCCTGAGCGAAACAGATCGTGGTTCAGGCGGGTTTGGGTCCACAGGAGGCGATTGA

Upstream 100 bases:

>100_bases
TAGATGAAAAGGGCGCTGACACCTGGCCCAGAATGTCCAAAGACGCAGTGTCGCAACGCCTCGCTGCCCTGATCGCTCAG
CGGTTGGCTTAACGGAGGCC

Downstream 100 bases:

>100_bases
TGCTATTGGTTTTTGGGCTATGCGCCGTTTTATGGGGCATTGGCAGCCTTATGGGCGCGCCGCGTTCCGCCCGCGCAATG
ATGATCGGCATCCTGTATGT

Product: deoxyuridine 5'-triphosphate nucleotidohydrolase

Products: NA

Alternate protein names: dUTPase; dUTP pyrophosphatase

Number of amino acids: Translated: 152; Mature: 151

Protein sequence:

>152_residues
MALVSLKLQWADGADTSVPLPAYETSGAAGADIRANCPDGPVTLAPGARALVPTGLRMAIPQGYEVQIRPRSGLALRHGI
TLVNSPGTIDSDYRGAVGVIMQNLGDAAFEITHGMRIAQMVVAPVVQASFELSDSLSETDRGSGGFGSTGGD

Sequences:

>Translated_152_residues
MALVSLKLQWADGADTSVPLPAYETSGAAGADIRANCPDGPVTLAPGARALVPTGLRMAIPQGYEVQIRPRSGLALRHGI
TLVNSPGTIDSDYRGAVGVIMQNLGDAAFEITHGMRIAQMVVAPVVQASFELSDSLSETDRGSGGFGSTGGD
>Mature_151_residues
ALVSLKLQWADGADTSVPLPAYETSGAAGADIRANCPDGPVTLAPGARALVPTGLRMAIPQGYEVQIRPRSGLALRHGIT
LVNSPGTIDSDYRGAVGVIMQNLGDAAFEITHGMRIAQMVVAPVVQASFELSDSLSETDRGSGGFGSTGGD

Specific function: This enzyme is involved in nucleotide metabolism:it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA

COG id: COG0756

COG function: function code F; dUTPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dUTPase family

Homologues:

Organism=Homo sapiens, GI70906444, Length=132, Percent_Identity=40.1515151515151, Blast_Score=96, Evalue=1e-20,
Organism=Homo sapiens, GI4503423, Length=110, Percent_Identity=43.6363636363636, Blast_Score=96, Evalue=1e-20,
Organism=Homo sapiens, GI70906441, Length=110, Percent_Identity=43.6363636363636, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI1790071, Length=135, Percent_Identity=50.3703703703704, Blast_Score=122, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI71988561, Length=130, Percent_Identity=43.0769230769231, Blast_Score=105, Evalue=7e-24,
Organism=Saccharomyces cerevisiae, GI6319729, Length=146, Percent_Identity=36.986301369863, Blast_Score=82, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24583610, Length=150, Percent_Identity=34.6666666666667, Blast_Score=94, Evalue=4e-20,
Organism=Drosophila melanogaster, GI19921126, Length=150, Percent_Identity=34.6666666666667, Blast_Score=94, Evalue=5e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DUT_ROSDO (Q16AZ3)

Other databases:

- EMBL:   CP000362
- RefSeq:   YP_681536.1
- ProteinModelPortal:   Q16AZ3
- SMR:   Q16AZ3
- STRING:   Q16AZ3
- GeneID:   4197488
- GenomeReviews:   CP000362_GR
- KEGG:   rde:RD1_1201
- NMPDR:   fig|375451.6.peg.1120
- eggNOG:   COG0756
- HOGENOM:   HBG436079
- OMA:   LDLRACI
- PhylomeDB:   Q16AZ3
- BioCyc:   RDEN375451:RD1_1201-MONOMER
- HAMAP:   MF_00116
- InterPro:   IPR008180
- InterPro:   IPR008181
- TIGRFAMs:   TIGR00576

Pfam domain/function: PF00692 dUTPase

EC number: =3.6.1.23

Molecular weight: Translated: 15594; Mature: 15463

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: NA

Important sites: BINDING 84-84

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALVSLKLQWADGADTSVPLPAYETSGAAGADIRANCPDGPVTLAPGARALVPTGLRMAI
CEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCEECCCCCCCEEECCCCCEECCCCEEEEC
PQGYEVQIRPRSGLALRHGITLVNSPGTIDSDYRGAVGVIMQNLGDAAFEITHGMRIAQM
CCCCEEEEECCCCEEEECCEEEEECCCCCCCCCCCHHHHHHHHCCCCEEHHHCCHHHHHH
VVAPVVQASFELSDSLSETDRGSGGFGSTGGD
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
ALVSLKLQWADGADTSVPLPAYETSGAAGADIRANCPDGPVTLAPGARALVPTGLRMAI
EEEEEEEEECCCCCCCCCCCCCCCCCCCCCCEECCCCCCCEEECCCCCEECCCCEEEEC
PQGYEVQIRPRSGLALRHGITLVNSPGTIDSDYRGAVGVIMQNLGDAAFEITHGMRIAQM
CCCCEEEEECCCCEEEECCEEEEECCCCCCCCCCCHHHHHHHHCCCCEEHHHCCHHHHHH
VVAPVVQASFELSDSLSETDRGSGGFGSTGGD
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA