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The map label for this gene is mtkB [H]

Identifier: 110678493

GI number: 110678493

Start: 1123954

End: 1124841

Strand: Direct

Name: mtkB [H]

Synonym: RD1_1163

Alternate gene names: 110678493

Gene position: 1123954-1124841 (Clockwise)

Preceding gene: 110678492

Following gene: 110678494

Centisome position: 27.19

GC content: 59.91

Gene sequence:

>888_bases
ATGAGCATACTTCTTGATAGCGACACCAAAGTCATTGTGCAGGGCATCACCGGCAAGATGGCCCGCTTCCACACCAAGGA
TATGCTCGAATACGGCACTAACGTCGTCGGTGGCGTGGTCCCTGGCAAGGGCGGCGAAACGGTCGAAGGCGTGCCTGTCT
TTGACACCGTTGAAGAGGCGGTCGCGCAAACCGGGGCGGAGGCGTCGCTGGTTTTCGTCCCGCCGCCTTTCGCGGCTGAT
AGCATTATGGAAGCCGCAGATGCGGGAATTCAGTACTGCGTCTGCATCACGGACGGTATCCCCGCACAGGACATGATCCG
TGTGAAACGCTACATGTACCGCTATCCGAAAGAGCGCCGCATGGTGCTCACCGGACCAAACTGTGCCGGCACGATCAGCC
CGGGCAAGGCGCTGCTGGGCATCATGCCGGGGCATATCTACCTGCAAGGCCACGTGGGTATCGTGGGACGTTCAGGCACG
CTGGGCTATGAGGCGGCCGCACAACTCAAGGAACGCGGCATCGGTGTTTCCACTTCGGTGGGGATTGGCGGCGACCCGAT
CAATGGTTCATCGTTCAAAGACATTCTGCAGCGTTTTGAAGAAGACGAAGACACCCATGTGATTGCGATGATCGGAGAGA
TCGGCGGACCGCAAGAAGCGGAAGCAGCCGAATACATCCGCGCGCATGTGACCAAACCGGTTGTGGCCTATGTCGCGGGC
CTGACCGCTCCTAAGGGGCGCACCATGGGCCATGCGGGCGCGATCATCTCGGCCTTTGGCGAAAGTGCGAGCGAAAAGGT
CGAAATCCTGTCGGCGGCAGGCGTCACGGTTGCCGAAAACCCGGCTGTCATCGGTGAGACAATCGCCAAAGTGATGGAGG
CCGCGTGA

Upstream 100 bases:

>100_bases
TGCCGATCATCCGCGCAACCACTCTGATGGAAGCCGCCGAGCGCGCGGTCGGTGCGTGGCAGAATGACCTGTCGCAGGGT
ACAAGAATGAGGGCCGTATC

Downstream 100 bases:

>100_bases
TGGTCAAGCCAAGTGTCCTCGTCACACGTCGCTGGCCCAAGATCGTCGAGCGCCAGCTGTCGGAAAAATACAACGTGCAG
CTCAACCGGACGGACCGCCC

Product: succinyl-CoA synthetase subunit alpha

Products: NA

Alternate protein names: MTK-alpha; Malate thiokinase; Malyl-CoA synthetase [H]

Number of amino acids: Translated: 295; Mature: 294

Protein sequence:

>295_residues
MSILLDSDTKVIVQGITGKMARFHTKDMLEYGTNVVGGVVPGKGGETVEGVPVFDTVEEAVAQTGAEASLVFVPPPFAAD
SIMEAADAGIQYCVCITDGIPAQDMIRVKRYMYRYPKERRMVLTGPNCAGTISPGKALLGIMPGHIYLQGHVGIVGRSGT
LGYEAAAQLKERGIGVSTSVGIGGDPINGSSFKDILQRFEEDEDTHVIAMIGEIGGPQEAEAAEYIRAHVTKPVVAYVAG
LTAPKGRTMGHAGAIISAFGESASEKVEILSAAGVTVAENPAVIGETIAKVMEAA

Sequences:

>Translated_295_residues
MSILLDSDTKVIVQGITGKMARFHTKDMLEYGTNVVGGVVPGKGGETVEGVPVFDTVEEAVAQTGAEASLVFVPPPFAAD
SIMEAADAGIQYCVCITDGIPAQDMIRVKRYMYRYPKERRMVLTGPNCAGTISPGKALLGIMPGHIYLQGHVGIVGRSGT
LGYEAAAQLKERGIGVSTSVGIGGDPINGSSFKDILQRFEEDEDTHVIAMIGEIGGPQEAEAAEYIRAHVTKPVVAYVAG
LTAPKGRTMGHAGAIISAFGESASEKVEILSAAGVTVAENPAVIGETIAKVMEAA
>Mature_294_residues
SILLDSDTKVIVQGITGKMARFHTKDMLEYGTNVVGGVVPGKGGETVEGVPVFDTVEEAVAQTGAEASLVFVPPPFAADS
IMEAADAGIQYCVCITDGIPAQDMIRVKRYMYRYPKERRMVLTGPNCAGTISPGKALLGIMPGHIYLQGHVGIVGRSGTL
GYEAAAQLKERGIGVSTSVGIGGDPINGSSFKDILQRFEEDEDTHVIAMIGEIGGPQEAEAAEYIRAHVTKPVVAYVAGL
TAPKGRTMGHAGAIISAFGESASEKVEILSAAGVTVAENPAVIGETIAKVMEAA

Specific function: Unknown

COG id: COG0074

COG function: function code C; Succinyl-CoA synthetase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the succinate/malate CoA ligase alpha subunit family [H]

Homologues:

Organism=Homo sapiens, GI109452591, Length=295, Percent_Identity=54.5762711864407, Blast_Score=273, Evalue=1e-73,
Organism=Homo sapiens, GI38569423, Length=257, Percent_Identity=30.3501945525292, Blast_Score=92, Evalue=6e-19,
Organism=Homo sapiens, GI38569421, Length=257, Percent_Identity=30.3501945525292, Blast_Score=92, Evalue=7e-19,
Organism=Escherichia coli, GI1786949, Length=293, Percent_Identity=56.6552901023891, Blast_Score=315, Evalue=2e-87,
Organism=Caenorhabditis elegans, GI17553126, Length=295, Percent_Identity=50.1694915254237, Blast_Score=270, Evalue=8e-73,
Organism=Caenorhabditis elegans, GI17550194, Length=292, Percent_Identity=51.7123287671233, Blast_Score=268, Evalue=3e-72,
Organism=Caenorhabditis elegans, GI17551266, Length=320, Percent_Identity=27.5, Blast_Score=98, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17557344, Length=319, Percent_Identity=26.0188087774295, Blast_Score=92, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6324716, Length=295, Percent_Identity=45.4237288135593, Blast_Score=239, Evalue=4e-64,
Organism=Drosophila melanogaster, GI21356559, Length=295, Percent_Identity=48.135593220339, Blast_Score=265, Evalue=3e-71,
Organism=Drosophila melanogaster, GI28574296, Length=296, Percent_Identity=47.972972972973, Blast_Score=241, Evalue=3e-64,
Organism=Drosophila melanogaster, GI281363477, Length=255, Percent_Identity=27.4509803921569, Blast_Score=85, Evalue=6e-17,
Organism=Drosophila melanogaster, GI281363475, Length=317, Percent_Identity=26.4984227129338, Blast_Score=85, Evalue=6e-17,
Organism=Drosophila melanogaster, GI281363473, Length=317, Percent_Identity=26.4984227129338, Blast_Score=85, Evalue=6e-17,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017440
- InterPro:   IPR003781
- InterPro:   IPR005810
- InterPro:   IPR005811
- InterPro:   IPR016040
- InterPro:   IPR016102 [H]

Pfam domain/function: PF02629 CoA_binding; PF00549 Ligase_CoA [H]

EC number: =6.2.1.9 [H]

Molecular weight: Translated: 30735; Mature: 30603

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00399 SUCCINYL_COA_LIG_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSILLDSDTKVIVQGITGKMARFHTKDMLEYGTNVVGGVVPGKGGETVEGVPVFDTVEEA
CEEEECCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
VAQTGAEASLVFVPPPFAADSIMEAADAGIQYCVCITDGIPAQDMIRVKRYMYRYPKERR
HHHCCCCEEEEEECCCCCHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCE
MVLTGPNCAGTISPGKALLGIMPGHIYLQGHVGIVGRSGTLGYEAAAQLKERGIGVSTSV
EEEECCCCCCCCCCCHHEEEECCCEEEEEEEEEEEECCCCCCHHHHHHHHHCCCCCEEEC
GIGGDPINGSSFKDILQRFEEDEDTHVIAMIGEIGGPQEAEAAEYIRAHVTKPVVAYVAG
CCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCHHHHHHHHH
LTAPKGRTMGHAGAIISAFGESASEKVEILSAAGVTVAENPAVIGETIAKVMEAA
CCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
SILLDSDTKVIVQGITGKMARFHTKDMLEYGTNVVGGVVPGKGGETVEGVPVFDTVEEA
EEEECCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
VAQTGAEASLVFVPPPFAADSIMEAADAGIQYCVCITDGIPAQDMIRVKRYMYRYPKERR
HHHCCCCEEEEEECCCCCHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCE
MVLTGPNCAGTISPGKALLGIMPGHIYLQGHVGIVGRSGTLGYEAAAQLKERGIGVSTSV
EEEECCCCCCCCCCCHHEEEECCCEEEEEEEEEEEECCCCCCHHHHHHHHHCCCCCEEEC
GIGGDPINGSSFKDILQRFEEDEDTHVIAMIGEIGGPQEAEAAEYIRAHVTKPVVAYVAG
CCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCHHHHHHHHH
LTAPKGRTMGHAGAIISAFGESASEKVEILSAAGVTVAENPAVIGETIAKVMEAA
CCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7961516 [H]