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The map label for this gene is thiD [H]

Identifier: 110678109

GI number: 110678109

Start: 720200

End: 720979

Strand: Direct

Name: thiD [H]

Synonym: RD1_0746

Alternate gene names: 110678109

Gene position: 720200-720979 (Clockwise)

Preceding gene: 110678108

Following gene: 110678110

Centisome position: 17.43

GC content: 58.46

Gene sequence:

>780_bases
ATGGTAGCAAGGGCGTTAACGATTGCTGGTTCCGACTCTGGTGGCGGGGCTGGTATTCAGGCCGACCTCAAGGCTTTTTC
GGCTATGGGGGTTTATGCCGCCTCCGTTGTGACGGCGGTTACCGCACAGAACACCACAGCCGTTACAGCCATTCATCCTA
TCCCGGACGGGGTCGTCGCTGCGCAGATCAAAGCCGTTCTGAATGATATCGATATAGACGCGATCAAGATCGGGATGCTG
GGCACACCGTCGCTGATCGCAGCCGTAGCGGATGCGCTGGCCGGCTATGATGGGCCGGTTGTGCTTGATCCTGTGATGGT
TGCTAAATCAGGTGATACGCTGCTGGCCGATCATGCGATAAATGCGCTGAAATCTTCACTTTTCGGACGCGCGACCCTGC
TGACACCTAACCTGCCTGAAGCGGCGAAACTGGTTGGCGCGGGCAGCACGCAGTCGCAGGCGGCCGCCCTTCTGGAGCAT
GGCGTCGGTGCAGTTCTGATGAAAGGCGGACATGCAGACGGAGAGACCTGCACCGACCATCTTGTCTCGCAAACAGGAAC
GCAAGAGTTTTCGGCCCCACGGGTGAATACACCTAACACCCACGGCACGGGATGTTCCTATTCCTCTGCGATCGCGGCGG
GTCTCGCGCAGGGTATGGCGTTGAGCGATGCGGTATCGCGTGCGCACAGGTGGCTACATCAAGCGATTTTGCATGCCGAT
GACTTGCACGTCGGGCAGGGCCATGGGCCCGTTCATCACTTTCACGCGTTTTGGAAATGA

Upstream 100 bases:

>100_bases
CTGAACCGGTTAGGACCGGCGGAGGGAACAGCTTGCGGAACTCGCATCCGTTTTGTCCTGCTCCCTTCAGCTTCCTGACC
CCTAAACCGAGGGCTACGAC

Downstream 100 bases:

>100_bases
GCGGCCCATTCACCATTCTGGGTGGCGGCGTTTGCGGGTTGGCAGTGGCGGCAGAATTGTCGTCACGCGGCGCGCAGGTC
ACACTCATTGATCCCAAGGG

Product: phosphomethylpyrimidine kinase

Products: NA

Alternate protein names: Hydroxymethylpyrimidine kinase; HMP kinase; Hydroxymethylpyrimidine phosphate kinase; HMP-P kinase; HMP-phosphate kinase; HMPP kinase [H]

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MVARALTIAGSDSGGGAGIQADLKAFSAMGVYAASVVTAVTAQNTTAVTAIHPIPDGVVAAQIKAVLNDIDIDAIKIGML
GTPSLIAAVADALAGYDGPVVLDPVMVAKSGDTLLADHAINALKSSLFGRATLLTPNLPEAAKLVGAGSTQSQAAALLEH
GVGAVLMKGGHADGETCTDHLVSQTGTQEFSAPRVNTPNTHGTGCSYSSAIAAGLAQGMALSDAVSRAHRWLHQAILHAD
DLHVGQGHGPVHHFHAFWK

Sequences:

>Translated_259_residues
MVARALTIAGSDSGGGAGIQADLKAFSAMGVYAASVVTAVTAQNTTAVTAIHPIPDGVVAAQIKAVLNDIDIDAIKIGML
GTPSLIAAVADALAGYDGPVVLDPVMVAKSGDTLLADHAINALKSSLFGRATLLTPNLPEAAKLVGAGSTQSQAAALLEH
GVGAVLMKGGHADGETCTDHLVSQTGTQEFSAPRVNTPNTHGTGCSYSSAIAAGLAQGMALSDAVSRAHRWLHQAILHAD
DLHVGQGHGPVHHFHAFWK
>Mature_259_residues
MVARALTIAGSDSGGGAGIQADLKAFSAMGVYAASVVTAVTAQNTTAVTAIHPIPDGVVAAQIKAVLNDIDIDAIKIGML
GTPSLIAAVADALAGYDGPVVLDPVMVAKSGDTLLADHAINALKSSLFGRATLLTPNLPEAAKLVGAGSTQSQAAALLEH
GVGAVLMKGGHADGETCTDHLVSQTGTQEFSAPRVNTPNTHGTGCSYSSAIAAGLAQGMALSDAVSRAHRWLHQAILHAD
DLHVGQGHGPVHHFHAFWK

Specific function: Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P. Shows no activity with pyridoxal, pyridoxamine or pyridoxine [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiD family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=261, Percent_Identity=51.3409961685824, Blast_Score=251, Evalue=3e-68,
Organism=Saccharomyces cerevisiae, GI6325378, Length=270, Percent_Identity=33.7037037037037, Blast_Score=122, Evalue=5e-29,
Organism=Saccharomyces cerevisiae, GI6324997, Length=271, Percent_Identity=34.3173431734317, Blast_Score=115, Evalue=7e-27,
Organism=Saccharomyces cerevisiae, GI6324517, Length=271, Percent_Identity=32.1033210332103, Blast_Score=107, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013749
- InterPro:   IPR004399 [H]

Pfam domain/function: PF08543 Phos_pyr_kin [H]

EC number: =2.7.1.49; =2.7.4.7 [H]

Molecular weight: Translated: 26166; Mature: 26166

Theoretical pI: Translated: 6.50; Mature: 6.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVARALTIAGSDSGGGAGIQADLKAFSAMGVYAASVVTAVTAQNTTAVTAIHPIPDGVVA
CCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHH
AQIKAVLNDIDIDAIKIGMLGTPSLIAAVADALAGYDGPVVLDPVMVAKSGDTLLADHAI
HHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHCCCCCEEECCEEEECCCCEEHHHHHH
NALKSSLFGRATLLTPNLPEAAKLVGAGSTQSQAAALLEHGVGAVLMKGGHADGETCTDH
HHHHHHHCCCEEEECCCCCHHHHHHCCCCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHH
LVSQTGTQEFSAPRVNTPNTHGTGCSYSSAIAAGLAQGMALSDAVSRAHRWLHQAILHAD
HHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHC
DLHVGQGHGPVHHFHAFWK
CCCCCCCCCCHHHHHHHCC
>Mature Secondary Structure
MVARALTIAGSDSGGGAGIQADLKAFSAMGVYAASVVTAVTAQNTTAVTAIHPIPDGVVA
CCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHH
AQIKAVLNDIDIDAIKIGMLGTPSLIAAVADALAGYDGPVVLDPVMVAKSGDTLLADHAI
HHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHCCCCCEEECCEEEECCCCEEHHHHHH
NALKSSLFGRATLLTPNLPEAAKLVGAGSTQSQAAALLEHGVGAVLMKGGHADGETCTDH
HHHHHHHCCCEEEECCCCCHHHHHHCCCCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHH
LVSQTGTQEFSAPRVNTPNTHGTGCSYSSAIAAGLAQGMALSDAVSRAHRWLHQAILHAD
HHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHC
DLHVGQGHGPVHHFHAFWK
CCCCCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10075431; 9097040; 9278503 [H]