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The map label for this gene is thiO [H]

Identifier: 110678110

GI number: 110678110

Start: 720976

End: 721956

Strand: Direct

Name: thiO [H]

Synonym: RD1_0747

Alternate gene names: 110678110

Gene position: 720976-721956 (Clockwise)

Preceding gene: 110678109

Following gene: 110678111

Centisome position: 17.44

GC content: 60.04

Gene sequence:

>981_bases
ATGAGCGGCCCATTCACCATTCTGGGTGGCGGCGTTTGCGGGTTGGCAGTGGCGGCAGAATTGTCGTCACGCGGCGCGCA
GGTCACACTCATTGATCCCAAGGGCGCGCCCGGCGATCATGCCTGTTCTTGGTGGGCCGGCGGCATGCTGGCGCCCGATT
GTGAAGGCGTGAGTGCTGAGCCCGAGATCATGCGTCAAGGCCGCAAAGCGGCAGGGTGGTGGGAAAGCCAAGGCGGGATT
GTAAATCACAAGGGCACGCTCGTTGTTGCGCTTAATCGCGATCAAAGCGATCTGGCAACTTTTGCGCGCCGTGCGCCGAC
CGCGACATCGCTGACAAAAACGCAGATCGCCGAGTACGAGCCGCTTTTGGCGGAACGTTTTTCCAAGGCGCTGTTCTTGG
CAGATGAGGGACATCTTGACCCTCGTGCAACACTGACCGGATTGCATGCGCGGCTGGAGCAAAGAGGCGTGGGCTTTGGC
GGCAATACCAACGGTCAGGTAATCGACTGCCGCGGGTTTGCTGCGCGCGATACCCTCACGGATTTGCGCGGCGTGAAGGG
CGAGATGCTTGTGGTCCGCTGCCCCGATGCGACGCTGTCGCGCCCCGTGCGGCTGCTTCACCCGCGATTTCCACTGTATA
TCGTGCCGCGTGGCGACAATGTCTTCATGTTGGGTGCGACCCAGATCGAAACCGGCGAACGCGGGCGCGCGACGCTGCGG
TCTGTTATGGAATTGATGAATGCGGCCTACGCCCTGCACCCCGCTTTTGGTGAGGCAGAGCTGTTGGAAATTGGCGTGGA
TGCACGCCCTGCCTTTCCAGACAACCAACCGCGCATCCGGCGGATTGGTGATGTGATTTATGTAAACGGTCTGTTTCGGC
ACGGATTTCTGCTTGCACCTGCACTAGCGCAGATGACGGCGGACCTGTTGCAAAAGGGCAAGATACCGGAGGTTTGGTAT
GAAGATCATCGTGAACGGTGA

Upstream 100 bases:

>100_bases
CGCGTGCGCACAGGTGGCTACATCAAGCGATTTTGCATGCCGATGACTTGCACGTCGGGCAGGGCCATGGGCCCGTTCAT
CACTTTCACGCGTTTTGGAA

Downstream 100 bases:

>100_bases
AGCACTTGAGACAGGCGCGCCCACACTGGCCGCGCTCTTGGAAGAGCTTGGGAAGGCTGAGGCCAAGGTGGCCACCTCGC
TGAACGAGACATTCGTGTCC

Product: thiamine biosynthesis oxidoreductase ThiO

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 326; Mature: 325

Protein sequence:

>326_residues
MSGPFTILGGGVCGLAVAAELSSRGAQVTLIDPKGAPGDHACSWWAGGMLAPDCEGVSAEPEIMRQGRKAAGWWESQGGI
VNHKGTLVVALNRDQSDLATFARRAPTATSLTKTQIAEYEPLLAERFSKALFLADEGHLDPRATLTGLHARLEQRGVGFG
GNTNGQVIDCRGFAARDTLTDLRGVKGEMLVVRCPDATLSRPVRLLHPRFPLYIVPRGDNVFMLGATQIETGERGRATLR
SVMELMNAAYALHPAFGEAELLEIGVDARPAFPDNQPRIRRIGDVIYVNGLFRHGFLLAPALAQMTADLLQKGKIPEVWY
EDHRER

Sequences:

>Translated_326_residues
MSGPFTILGGGVCGLAVAAELSSRGAQVTLIDPKGAPGDHACSWWAGGMLAPDCEGVSAEPEIMRQGRKAAGWWESQGGI
VNHKGTLVVALNRDQSDLATFARRAPTATSLTKTQIAEYEPLLAERFSKALFLADEGHLDPRATLTGLHARLEQRGVGFG
GNTNGQVIDCRGFAARDTLTDLRGVKGEMLVVRCPDATLSRPVRLLHPRFPLYIVPRGDNVFMLGATQIETGERGRATLR
SVMELMNAAYALHPAFGEAELLEIGVDARPAFPDNQPRIRRIGDVIYVNGLFRHGFLLAPALAQMTADLLQKGKIPEVWY
EDHRER
>Mature_325_residues
SGPFTILGGGVCGLAVAAELSSRGAQVTLIDPKGAPGDHACSWWAGGMLAPDCEGVSAEPEIMRQGRKAAGWWESQGGIV
NHKGTLVVALNRDQSDLATFARRAPTATSLTKTQIAEYEPLLAERFSKALFLADEGHLDPRATLTGLHARLEQRGVGFGG
NTNGQVIDCRGFAARDTLTDLRGVKGEMLVVRCPDATLSRPVRLLHPRFPLYIVPRGDNVFMLGATQIETGERGRATLRS
VMELMNAAYALHPAFGEAELLEIGVDARPAFPDNQPRIRRIGDVIYVNGLFRHGFLLAPALAQMTADLLQKGKIPEVWYE
DHRER

Specific function: May have amino acid oxidase activity in the biosynthesis of thiazole when cysteine gives its thiol group to be inserted into the thiazole molecule and the rest of the molecule is deaminated to give pyruvic acid and ammonia [H]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076
- InterPro:   IPR012727 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: NA

Molecular weight: Translated: 35359; Mature: 35228

Theoretical pI: Translated: 7.22; Mature: 7.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGPFTILGGGVCGLAVAAELSSRGAQVTLIDPKGAPGDHACSWWAGGMLAPDCEGVSAE
CCCCEEEECCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHCCCCCCCCCCCCCCC
PEIMRQGRKAAGWWESQGGIVNHKGTLVVALNRDQSDLATFARRAPTATSLTKTQIAEYE
HHHHHCCCCCCCCCCCCCCEEECCCEEEEEECCCHHHHHHHHHCCCCCHHHHHHHHHHCC
PLLAERFSKALFLADEGHLDPRATLTGLHARLEQRGVGFGGNTNGQVIDCRGFAARDTLT
HHHHHHHHHEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCHHHHHH
DLRGVKGEMLVVRCPDATLSRPVRLLHPRFPLYIVPRGDNVFMLGATQIETGERGRATLR
HHHCCCCCEEEEECCCHHHHCCHHHHCCCCCEEEEECCCCEEEEEEEEECCCCCHHHHHH
SVMELMNAAYALHPAFGEAELLEIGVDARPAFPDNQPRIRRIGDVIYVNGLFRHGFLLAP
HHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHCCEEEEECHHHCCHHHHH
ALAQMTADLLQKGKIPEVWYEDHRER
HHHHHHHHHHHCCCCCHHHHHHCCCC
>Mature Secondary Structure 
SGPFTILGGGVCGLAVAAELSSRGAQVTLIDPKGAPGDHACSWWAGGMLAPDCEGVSAE
CCCEEEECCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHCCCCCCCCCCCCCCC
PEIMRQGRKAAGWWESQGGIVNHKGTLVVALNRDQSDLATFARRAPTATSLTKTQIAEYE
HHHHHCCCCCCCCCCCCCCEEECCCEEEEEECCCHHHHHHHHHCCCCCHHHHHHHHHHCC
PLLAERFSKALFLADEGHLDPRATLTGLHARLEQRGVGFGGNTNGQVIDCRGFAARDTLT
HHHHHHHHHEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCHHHHHH
DLRGVKGEMLVVRCPDATLSRPVRLLHPRFPLYIVPRGDNVFMLGATQIETGERGRATLR
HHHCCCCCEEEEECCCHHHHCCHHHHCCCCCEEEEECCCCEEEEEEEEECCCCCHHHHHH
SVMELMNAAYALHPAFGEAELLEIGVDARPAFPDNQPRIRRIGDVIYVNGLFRHGFLLAP
HHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHCCEEEEECHHHCCHHHHH
ALAQMTADLLQKGKIPEVWYEDHRER
HHHHHHHHHHHCCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371431 [H]