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The map label for this gene is galR [H]

Identifier: 108811905

GI number: 108811905

Start: 1964351

End: 1965358

Strand: Reverse

Name: galR [H]

Synonym: YPN_1743

Alternate gene names: 108811905

Gene position: 1965358-1964351 (Counterclockwise)

Preceding gene: 108811906

Following gene: 108811904

Centisome position: 43.34

GC content: 49.21

Gene sequence:

>1008_bases
ATGAAAGATGTTGCGCTGAGGGCTGGGGTTTCAGTAGCAACGGTCAGTCGTGTTCTTAATAATACGGCCTATGTAGAGCC
AGGTACCCGTGAACGTGTAGAGAAGGCAATGCGTGAGTACAACTACCAGCGAAATGCGGCAGCTCTCGCGTTGGCTAAAA
GAAGTGGAAACATGCTGGGCTTACTTACCGGTAATCTGGCTGATCCATTCTTCTCTCGTTTAGCCCGAGGGGTTGAAGAG
GTTGCTCGTAAAAAAGGAGCAAAATTGATGGTCTGCAGTGGGGGCCATCAGGAAGAACTGGAAAAAGCAGGGCTCGATTT
TTTGATCAATCAAGGATGCGAAGCCATCGTTGCGCACATCACACGAATGAGCGAGGCTGAAATCTTGCGCTATGCTGCTC
ACACTCCTGGGCTGGTACTCATTAATCGTTATTTACCCGCTATCGCTAACCGGTGTGTCTGGTTAGACAATACCCGTGCC
GCAGAAGCCTCCACCCATTATCTACTTCAGAATGGTCATACCCGAATTGCCTGTGTCACAGCCAAGTTATCGATTGATGA
CAAAAAACAGCGCTTGGAAGGGTACCGAGCCGCCATGGCCAGCGCGGGGATCACGGTGCCTGCTGATTGGATTATTAGTG
TTCCGTTTGATGAGGAGGGGGGCGAATTAGCGGCAGAGAAACTATTAGCCAGTGATCAGAACTTTACCGCAGTGGTGACA
TTTAACGATGTGATGGCTGCCGGGATGATGCGGTCATTACATCAAAGAAACATCAGCCTGCCAGAACAACTTTCAATTGT
TGGTTTTGATGATGTCGTATTGGCCCGCTATCTCTATCCATCATTAACTACCGTCCATTACCCGATTGAGCGAATGGCCA
GAAGGGCTGCAAACATCGCTTTGCTGTTACATGCTCAGGGGGAAGTCTCTCCTCAAATCAATATGTTCTCCGCTGACCTG
GTTATCAGGGATTCAGTTGCACAGGTAGGGAAGAATAGGGGGAAATAG

Upstream 100 bases:

>100_bases
TACATTCAATGGTGTTGTGGTGACATGAGTTGCTATCGATGTTCGGTGGTTATAAAACAGCTAAAGAGAGTGCCTAAATG
GGGAGTGACGTGTGGCCACA

Downstream 100 bases:

>100_bases
GGCAATCAATAGCTAAAAACGTGACTATCATCACCAATATAAATCGGAACCTTACGTTATATTAAATGCCACACCAGGGA
AATTACACAATCTCGTTTAC

Product: LacI family transcriptional regulator

Products: NA

Alternate protein names: Galactose operon repressor [H]

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MKDVALRAGVSVATVSRVLNNTAYVEPGTRERVEKAMREYNYQRNAAALALAKRSGNMLGLLTGNLADPFFSRLARGVEE
VARKKGAKLMVCSGGHQEELEKAGLDFLINQGCEAIVAHITRMSEAEILRYAAHTPGLVLINRYLPAIANRCVWLDNTRA
AEASTHYLLQNGHTRIACVTAKLSIDDKKQRLEGYRAAMASAGITVPADWIISVPFDEEGGELAAEKLLASDQNFTAVVT
FNDVMAAGMMRSLHQRNISLPEQLSIVGFDDVVLARYLYPSLTTVHYPIERMARRAANIALLLHAQGEVSPQINMFSADL
VIRDSVAQVGKNRGK

Sequences:

>Translated_335_residues
MKDVALRAGVSVATVSRVLNNTAYVEPGTRERVEKAMREYNYQRNAAALALAKRSGNMLGLLTGNLADPFFSRLARGVEE
VARKKGAKLMVCSGGHQEELEKAGLDFLINQGCEAIVAHITRMSEAEILRYAAHTPGLVLINRYLPAIANRCVWLDNTRA
AEASTHYLLQNGHTRIACVTAKLSIDDKKQRLEGYRAAMASAGITVPADWIISVPFDEEGGELAAEKLLASDQNFTAVVT
FNDVMAAGMMRSLHQRNISLPEQLSIVGFDDVVLARYLYPSLTTVHYPIERMARRAANIALLLHAQGEVSPQINMFSADL
VIRDSVAQVGKNRGK
>Mature_335_residues
MKDVALRAGVSVATVSRVLNNTAYVEPGTRERVEKAMREYNYQRNAAALALAKRSGNMLGLLTGNLADPFFSRLARGVEE
VARKKGAKLMVCSGGHQEELEKAGLDFLINQGCEAIVAHITRMSEAEILRYAAHTPGLVLINRYLPAIANRCVWLDNTRA
AEASTHYLLQNGHTRIACVTAKLSIDDKKQRLEGYRAAMASAGITVPADWIISVPFDEEGGELAAEKLLASDQNFTAVVT
FNDVMAAGMMRSLHQRNISLPEQLSIVGFDDVVLARYLYPSLTTVHYPIERMARRAANIALLLHAQGEVSPQINMFSADL
VIRDSVAQVGKNRGK

Specific function: Repressor of the galactose operon. Binds galactose as an inducer [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789202, Length=327, Percent_Identity=42.8134556574924, Blast_Score=251, Evalue=4e-68,
Organism=Escherichia coli, GI1788474, Length=328, Percent_Identity=41.4634146341463, Blast_Score=246, Evalue=2e-66,
Organism=Escherichia coli, GI1789068, Length=329, Percent_Identity=34.0425531914894, Blast_Score=185, Evalue=4e-48,
Organism=Escherichia coli, GI1790369, Length=326, Percent_Identity=33.4355828220859, Blast_Score=180, Evalue=1e-46,
Organism=Escherichia coli, GI1790194, Length=307, Percent_Identity=33.5504885993485, Blast_Score=180, Evalue=1e-46,
Organism=Escherichia coli, GI1787948, Length=329, Percent_Identity=29.1793313069909, Blast_Score=140, Evalue=8e-35,
Organism=Escherichia coli, GI1787906, Length=289, Percent_Identity=29.4117647058824, Blast_Score=117, Evalue=8e-28,
Organism=Escherichia coli, GI1787580, Length=318, Percent_Identity=27.3584905660377, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI48994940, Length=318, Percent_Identity=27.3584905660377, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1789456, Length=337, Percent_Identity=25.5192878338279, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1786540, Length=336, Percent_Identity=28.5714285714286, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1790715, Length=306, Percent_Identity=26.1437908496732, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1786268, Length=282, Percent_Identity=24.822695035461, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36693; Mature: 36693

Theoretical pI: Translated: 8.84; Mature: 8.84

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDVALRAGVSVATVSRVLNNTAYVEPGTRERVEKAMREYNYQRNAAALALAKRSGNMLG
CCCCHHHCCCHHHHHHHHHCCCCEECCCCHHHHHHHHHHCCCCCCHHHHHEECCCCCEEE
LLTGNLADPFFSRLARGVEEVARKKGAKLMVCSGGHQEELEKAGLDFLINQGCEAIVAHI
EEECCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHCCHHHHHHCCHHHHHHHH
TRMSEAEILRYAAHTPGLVLINRYLPAIANRCVWLDNTRAAEASTHYLLQNGHTRIACVT
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHCCCCEEEEEEE
AKLSIDDKKQRLEGYRAAMASAGITVPADWIISVPFDEEGGELAAEKLLASDQNFTAVVT
EEECCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCHHHHHHHHHCCCCCEEEEE
FNDVMAAGMMRSLHQRNISLPEQLSIVGFDDVVLARYLYPSLTTVHYPIERMARRAANIA
EHHHHHHHHHHHHHHCCCCCCHHEEEECCHHHHHHHHHCCCHHHEECCHHHHHHHHCCEE
LLLHAQGEVSPQINMFSADLVIRDSVAQVGKNRGK
EEEEECCCCCCCEEEEEECEEEHHHHHHHHHCCCC
>Mature Secondary Structure
MKDVALRAGVSVATVSRVLNNTAYVEPGTRERVEKAMREYNYQRNAAALALAKRSGNMLG
CCCCHHHCCCHHHHHHHHHCCCCEECCCCHHHHHHHHHHCCCCCCHHHHHEECCCCCEEE
LLTGNLADPFFSRLARGVEEVARKKGAKLMVCSGGHQEELEKAGLDFLINQGCEAIVAHI
EEECCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHCCHHHHHHCCHHHHHHHH
TRMSEAEILRYAAHTPGLVLINRYLPAIANRCVWLDNTRAAEASTHYLLQNGHTRIACVT
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHCCCCEEEEEEE
AKLSIDDKKQRLEGYRAAMASAGITVPADWIISVPFDEEGGELAAEKLLASDQNFTAVVT
EEECCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCHHHHHHHHHCCCCCEEEEE
FNDVMAAGMMRSLHQRNISLPEQLSIVGFDDVVLARYLYPSLTTVHYPIERMARRAANIA
EHHHHHHHHHHHHHHCCCCCCHHEEEECCHHHHHHHHHCCCHHHEECCHHHHHHHHCCEE
LLLHAQGEVSPQINMFSADLVIRDSVAQVGKNRGK
EEEEECCCCCCCEEEEEECEEEHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6283521; 9278503; 6350601; 8188660; 8982002 [H]