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The map label for this gene is ispF [H]

Identifier: 108801701

GI number: 108801701

Start: 5016661

End: 5017137

Strand: Reverse

Name: ispF [H]

Synonym: Mmcs_4738

Alternate gene names: 108801701

Gene position: 5017137-5016661 (Counterclockwise)

Preceding gene: 108801702

Following gene: 108801700

Centisome position: 87.94

GC content: 72.75

Gene sequence:

>477_bases
GTGAGCCTGCCGCGTGTCGGTCTCGGCACCGACGTGCACCCGATCGAGCCGGGCCGGCCCTGCCGGCTGCTCGGTCTGCA
CTTCGACGGCGAAGACGGGTGCGCCGGACATTCCGACGGCGACGTCGCCGCCCACGCACTCTGCGACGCCCTGCTGTCGG
CGGCCGGGCTCGGCGACCTCGGTGGCGTCTTCGGCACCGGGCGGCCCGAATGGGAGGGCGTCAGCGGCGCCGACATGCTG
CGCCACGTCCGGGGCCTGCTGCAGGAAGCGGGATTTCGGGTCGGCAATGCGACCGTCCAGGTGATCGGCAACCGGCCGAA
GATCGGCCCGCGGCGGGCCGAGGCCCAGGAGTTGCTGTCCGAACTGATCGGCGCCCCGGTCTCGGTTTCGGCGACGACGA
CCGACGGGCTCGGGCTGACCGGCCGTGGTGAGGGGCTGGCCGCGATCGCAACCGCTCTCGTGGTCGATGAGGGATAA

Upstream 100 bases:

>100_bases
CCGGCGGGCAGGTGCAGGTCGTCGAGGGCGACCCACTGGCCTTCAAGATCACCACCCCACTCGACCTCGTGCTGGCCGAG
GCGATCCTGGCGGGCGCCCG

Downstream 100 bases:

>100_bases
CGGCATATCGGCCGGTAAGCTGGCACGTCGTGACCGATCGCGCTGATGCCGACCGGCCGGCGACGTCGCCGACGGGTCTG
CGCCTCTACGACACCATGAC

Product: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS [H]

Number of amino acids: Translated: 158; Mature: 157

Protein sequence:

>158_residues
MSLPRVGLGTDVHPIEPGRPCRLLGLHFDGEDGCAGHSDGDVAAHALCDALLSAAGLGDLGGVFGTGRPEWEGVSGADML
RHVRGLLQEAGFRVGNATVQVIGNRPKIGPRRAEAQELLSELIGAPVSVSATTTDGLGLTGRGEGLAAIATALVVDEG

Sequences:

>Translated_158_residues
MSLPRVGLGTDVHPIEPGRPCRLLGLHFDGEDGCAGHSDGDVAAHALCDALLSAAGLGDLGGVFGTGRPEWEGVSGADML
RHVRGLLQEAGFRVGNATVQVIGNRPKIGPRRAEAQELLSELIGAPVSVSATTTDGLGLTGRGEGLAAIATALVVDEG
>Mature_157_residues
SLPRVGLGTDVHPIEPGRPCRLLGLHFDGEDGCAGHSDGDVAAHALCDALLSAAGLGDLGGVFGTGRPEWEGVSGADMLR
HVRGLLQEAGFRVGNATVQVIGNRPKIGPRRAEAQELLSELIGAPVSVSATTTDGLGLTGRGEGLAAIATALVVDEG

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP [H]

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family [H]

Homologues:

Organism=Escherichia coli, GI1789103, Length=154, Percent_Identity=41.5584415584416, Blast_Score=107, Evalue=4e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003526
- InterPro:   IPR020555 [H]

Pfam domain/function: PF02542 YgbB [H]

EC number: =4.6.1.12 [H]

Molecular weight: Translated: 15994; Mature: 15863

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLPRVGLGTDVHPIEPGRPCRLLGLHFDGEDGCAGHSDGDVAAHALCDALLSAAGLGDL
CCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCC
GGVFGTGRPEWEGVSGADMLRHVRGLLQEAGFRVGNATVQVIGNRPKIGPRRAEAQELLS
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCHHHHHHHHH
ELIGAPVSVSATTTDGLGLTGRGEGLAAIATALVVDEG
HHHCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCC
>Mature Secondary Structure 
SLPRVGLGTDVHPIEPGRPCRLLGLHFDGEDGCAGHSDGDVAAHALCDALLSAAGLGDL
CCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCC
GGVFGTGRPEWEGVSGADMLRHVRGLLQEAGFRVGNATVQVIGNRPKIGPRRAEAQELLS
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCHHHHHHHHH
ELIGAPVSVSATTTDGLGLTGRGEGLAAIATALVVDEG
HHHCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA