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The map label for this gene is ispD [H]

Identifier: 108801702

GI number: 108801702

Start: 5017134

End: 5017799

Strand: Reverse

Name: ispD [H]

Synonym: Mmcs_4739

Alternate gene names: 108801702

Gene position: 5017799-5017134 (Counterclockwise)

Preceding gene: 108801703

Following gene: 108801701

Centisome position: 87.95

GC content: 71.62

Gene sequence:

>666_bases
ATGACCACGGTGGCCGTTGTTCCGGCCGCGGGTTCCGGTCAACGGCTCGCGGCCGGCGCGCCCAAAGCGTTCGTGAATCT
CGCCGGGCGGCCGATGCTCGAACGCGCCATCGCCGGGTTACGGAATTCCGGTGTGGTCGACAGCATCGTGGTCGCCGTCC
CACCGAGCCGCACCGACGAAGCCAAGCTGGTATTCGGCGGTGAGGCGTTGATCGTCGCGGGCGGCGCCGACCGCACCGAA
TCGGTGAGAGCCGCGCTCGCCGCCGTCGGCGATGCCGACTACGTGCTCGTTCACGACGCTGCGCGCGCACTCACACCACC
ATCGCTGGTGGCCCGTGTCGTGCGGGCGCTGGAAGCCGGGCACAGCGCGGTGGTGCCCGGGCTCCCGGTGGCCGACACGA
TCAAGGCCGTCGACGCCAACGGCGCCGTACTCGGCACACCCGAGCGGGCCGGACTGCGCGCGATCCAGACCCCGCAGGGT
TTCCACATCGACGTGCTGCGCCGCGCGTACGCACGGGCGGGCGACGGCGGTTTCACCGACGACGCGTCGATGGTCGAGCA
GGCCGGCGGGCAGGTGCAGGTCGTCGAGGGCGACCCACTGGCCTTCAAGATCACCACCCCACTCGACCTCGTGCTGGCCG
AGGCGATCCTGGCGGGCGCCCGGTGA

Upstream 100 bases:

>100_bases
CAGATCCTGGTCGGTGAGCTGGCGCTCGCCGAGAACGTCGAAGACGAGAAGGCCACGATCATCCTCGACGAGGCGTTGGC
TGCCGCGTCCTGACGCACAC

Downstream 100 bases:

>100_bases
GCCTGCCGCGTGTCGGTCTCGGCACCGACGTGCACCCGATCGAGCCGGGCCGGCCCTGCCGGCTGCTCGGTCTGCACTTC
GACGGCGAAGACGGGTGCGC

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT [H]

Number of amino acids: Translated: 221; Mature: 220

Protein sequence:

>221_residues
MTTVAVVPAAGSGQRLAAGAPKAFVNLAGRPMLERAIAGLRNSGVVDSIVVAVPPSRTDEAKLVFGGEALIVAGGADRTE
SVRAALAAVGDADYVLVHDAARALTPPSLVARVVRALEAGHSAVVPGLPVADTIKAVDANGAVLGTPERAGLRAIQTPQG
FHIDVLRRAYARAGDGGFTDDASMVEQAGGQVQVVEGDPLAFKITTPLDLVLAEAILAGAR

Sequences:

>Translated_221_residues
MTTVAVVPAAGSGQRLAAGAPKAFVNLAGRPMLERAIAGLRNSGVVDSIVVAVPPSRTDEAKLVFGGEALIVAGGADRTE
SVRAALAAVGDADYVLVHDAARALTPPSLVARVVRALEAGHSAVVPGLPVADTIKAVDANGAVLGTPERAGLRAIQTPQG
FHIDVLRRAYARAGDGGFTDDASMVEQAGGQVQVVEGDPLAFKITTPLDLVLAEAILAGAR
>Mature_220_residues
TTVAVVPAAGSGQRLAAGAPKAFVNLAGRPMLERAIAGLRNSGVVDSIVVAVPPSRTDEAKLVFGGEALIVAGGADRTES
VRAALAAVGDADYVLVHDAARALTPPSLVARVVRALEAGHSAVVPGLPVADTIKAVDANGAVLGTPERAGLRAIQTPQGF
HIDVLRRAYARAGDGGFTDDASMVEQAGGQVQVVEGDPLAFKITTPLDLVLAEAILAGAR

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP) [H]

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family [H]

Homologues:

Organism=Homo sapiens, GI157412259, Length=230, Percent_Identity=27.8260869565217, Blast_Score=84, Evalue=1e-16,
Organism=Escherichia coli, GI1789104, Length=222, Percent_Identity=36.4864864864865, Blast_Score=119, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001228
- InterPro:   IPR018294 [H]

Pfam domain/function: PF01128 IspD [H]

EC number: =2.7.7.60 [H]

Molecular weight: Translated: 22322; Mature: 22191

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTVAVVPAAGSGQRLAAGAPKAFVNLAGRPMLERAIAGLRNSGVVDSIVVAVPPSRTDE
CCEEEEEECCCCCCEEECCCCHHHHHCCCCHHHHHHHHCCCCCCCCEEEEEEECCCCCCC
AKLVFGGEALIVAGGADRTESVRAALAAVGDADYVLVHDAARALTPPSLVARVVRALEAG
CEEEECCCEEEEECCCCCHHHHHHHHHHCCCCCEEEEECCHHHCCCHHHHHHHHHHHHCC
HSAVVPGLPVADTIKAVDANGAVLGTPERAGLRAIQTPQGFHIDVLRRAYARAGDGGFTD
CCCCCCCCCHHHHHHEECCCCCEEECCCHHCCEEECCCCCCHHHHHHHHHHHCCCCCCCC
DASMVEQAGGQVQVVEGDPLAFKITTPLDLVLAEAILAGAR
CHHHHHHCCCEEEEEECCCEEEEECCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
TTVAVVPAAGSGQRLAAGAPKAFVNLAGRPMLERAIAGLRNSGVVDSIVVAVPPSRTDE
CEEEEEECCCCCCEEECCCCHHHHHCCCCHHHHHHHHCCCCCCCCEEEEEEECCCCCCC
AKLVFGGEALIVAGGADRTESVRAALAAVGDADYVLVHDAARALTPPSLVARVVRALEAG
CEEEECCCEEEEECCCCCHHHHHHHHHHCCCCCEEEEECCHHHCCCHHHHHHHHHHHHCC
HSAVVPGLPVADTIKAVDANGAVLGTPERAGLRAIQTPQGFHIDVLRRAYARAGDGGFTD
CCCCCCCCCHHHHHHEECCCCCEEECCCHHCCEEECCCCCCHHHHHHHHHHHCCCCCCCC
DASMVEQAGGQVQVVEGDPLAFKITTPLDLVLAEAILAGAR
CHHHHHHCCCEEEEEECCCEEEEECCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA