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The map label for this gene is mhpB [H]

Identifier: 108800750

GI number: 108800750

Start: 4039544

End: 4040392

Strand: Reverse

Name: mhpB [H]

Synonym: Mmcs_3785

Alternate gene names: 108800750

Gene position: 4040392-4039544 (Counterclockwise)

Preceding gene: 108800751

Following gene: 108800749

Centisome position: 70.82

GC content: 69.49

Gene sequence:

>849_bases
TTGGCCGTCGCCCGTGATTTCGTCACCGAATTCGACCCGGACCTGGTGGTCACCTTCTCGCCGGACCACTACAACGGGTT
CTTCTACAAGGTCATGCCCCCGTTCTGCGTCGGCACCTCCGCGCAGGGCGTCGGGGACTACGGCACCCACGCCGGACCGC
TCGACGTGCCGGAGGACCTCGCGAACGAGCTGGCCACCGCAGTACTGGAAGCCGGTGTGGACGTGGCGATCTCGGCGAGT
ATGGACGTCGACCACGGCACCGTGCAGCCGCTGCAGAATCTGTTCGGCGATGCGATGGCCCGTCCCGTCATCCCGGTGTT
CATCAACTCCGTCGCCACCCCGCTGGGCCCGCTGCGGCGCACCCGTGCGCTGGGTACGGCGATCGGACGGTACCTCGCGA
CCCTCGACAAGCGTGTCCTGGTGATCGGTTCGGGTGGGTTGTCCCACGACCCGCCGGTGCCCACCCTGGCGACCGCCCCG
CCGGCGGCGCTCGACCGCATCGTGCACGGCGCACCGATGAGCACCGAACAGCGGATGGCCCGGCAGTCCGCGGTGATCGA
CGCGGCGCACGCGTTCGCGCACGGGGAAAGCCCCCTGCAGCCGCTCAATCCGGCGTGGGATGCGACGTTCCTGGAGATCC
TCGACGAGGGCTGGCTCTCGGATCTCGACGGCTGGTCCAACGCGTTCATCGCCCGCGAGGGTGGGAACTCCGCACACGAG
ATCCGCACCTGGGTAGCGGCTTTCGCGGCGCTGGCGGCCGGCGGTGACTACCGCACCGGCCTGCGCTTCTACCGTGCCGC
ACCCGAGTTGATCGCGGGTTTCGCGATCCGGACGGCGGTGCTCGCGTGA

Upstream 100 bases:

>100_bases
GGTCACCATGGCGCAGATCGCCCTGTGCTGCACGTCGCACAGTCCGCTACTGAACCTTCCGGGACCGTCGCGGGAACTCC
TTGACGACATCGGGTCGGCG

Downstream 100 bases:

>100_bases
ACTTCGACAGGCACGTCGACGTGCTGGTGGTCGGCTCGGGCGGCGGCGGGATGACCGCCGCGTTGACAGCTCACTCGTGC
GGGCTGGACACGCTGGTGGT

Product: 3-(2,3-dihydroxyphenyl)propionate dioxygenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 282; Mature: 281

Protein sequence:

>282_residues
MAVARDFVTEFDPDLVVTFSPDHYNGFFYKVMPPFCVGTSAQGVGDYGTHAGPLDVPEDLANELATAVLEAGVDVAISAS
MDVDHGTVQPLQNLFGDAMARPVIPVFINSVATPLGPLRRTRALGTAIGRYLATLDKRVLVIGSGGLSHDPPVPTLATAP
PAALDRIVHGAPMSTEQRMARQSAVIDAAHAFAHGESPLQPLNPAWDATFLEILDEGWLSDLDGWSNAFIAREGGNSAHE
IRTWVAAFAALAAGGDYRTGLRFYRAAPELIAGFAIRTAVLA

Sequences:

>Translated_282_residues
MAVARDFVTEFDPDLVVTFSPDHYNGFFYKVMPPFCVGTSAQGVGDYGTHAGPLDVPEDLANELATAVLEAGVDVAISAS
MDVDHGTVQPLQNLFGDAMARPVIPVFINSVATPLGPLRRTRALGTAIGRYLATLDKRVLVIGSGGLSHDPPVPTLATAP
PAALDRIVHGAPMSTEQRMARQSAVIDAAHAFAHGESPLQPLNPAWDATFLEILDEGWLSDLDGWSNAFIAREGGNSAHE
IRTWVAAFAALAAGGDYRTGLRFYRAAPELIAGFAIRTAVLA
>Mature_281_residues
AVARDFVTEFDPDLVVTFSPDHYNGFFYKVMPPFCVGTSAQGVGDYGTHAGPLDVPEDLANELATAVLEAGVDVAISASM
DVDHGTVQPLQNLFGDAMARPVIPVFINSVATPLGPLRRTRALGTAIGRYLATLDKRVLVIGSGGLSHDPPVPTLATAPP
AALDRIVHGAPMSTEQRMARQSAVIDAAHAFAHGESPLQPLNPAWDATFLEILDEGWLSDLDGWSNAFIAREGGNSAHEI
RTWVAAFAALAAGGDYRTGLRFYRAAPELIAGFAIRTAVLA

Specific function: Catalyzes the non-heme iron(II)-dependent oxidative cleavage of 2,3-dihydroxyphenylpropionic acid and 2,3- dihydroxicinnamic acid into 2-hydroxy-6-ketononadienedioate and 2- hydroxy-6-ketononatrienedioate, respectively [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ligB/mhpB extradiol dioxygenase family [H]

Homologues:

Organism=Escherichia coli, GI1786544, Length=280, Percent_Identity=51.7857142857143, Blast_Score=291, Evalue=3e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004183 [H]

Pfam domain/function: PF02900 LigB [H]

EC number: =1.13.11.16 [H]

Molecular weight: Translated: 29859; Mature: 29728

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVARDFVTEFDPDLVVTFSPDHYNGFFYKVMPPFCVGTSAQGVGDYGTHAGPLDVPEDL
CCCHHHHHHHCCCCEEEEECCCCCCCEEEEECCCHHCCCCCCCCCCCCCCCCCCCCHHHH
ANELATAVLEAGVDVAISASMDVDHGTVQPLQNLFGDAMARPVIPVFINSVATPLGPLRR
HHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHH
TRALGTAIGRYLATLDKRVLVIGSGGLSHDPPVPTLATAPPAALDRIVHGAPMSTEQRMA
HHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHH
RQSAVIDAAHAFAHGESPLQPLNPAWDATFLEILDEGWLSDLDGWSNAFIAREGGNSAHE
HHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHH
IRTWVAAFAALAAGGDYRTGLRFYRAAPELIAGFAIRTAVLA
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AVARDFVTEFDPDLVVTFSPDHYNGFFYKVMPPFCVGTSAQGVGDYGTHAGPLDVPEDL
CCHHHHHHHCCCCEEEEECCCCCCCEEEEECCCHHCCCCCCCCCCCCCCCCCCCCHHHH
ANELATAVLEAGVDVAISASMDVDHGTVQPLQNLFGDAMARPVIPVFINSVATPLGPLRR
HHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHH
TRALGTAIGRYLATLDKRVLVIGSGGLSHDPPVPTLATAPPAALDRIVHGAPMSTEQRMA
HHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHH
RQSAVIDAAHAFAHGESPLQPLNPAWDATFLEILDEGWLSDLDGWSNAFIAREGGNSAHE
HHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHH
IRTWVAAFAALAAGGDYRTGLRFYRAAPELIAGFAIRTAVLA
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA