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The map label for this gene is kstD [H]

Identifier: 108800749

GI number: 108800749

Start: 4037766

End: 4039547

Strand: Reverse

Name: kstD [H]

Synonym: Mmcs_3784

Alternate gene names: 108800749

Gene position: 4039547-4037766 (Counterclockwise)

Preceding gene: 108800750

Following gene: 108800748

Centisome position: 70.8

GC content: 68.74

Gene sequence:

>1782_bases
GTGAACTTCGACAGGCACGTCGACGTGCTGGTGGTCGGCTCGGGCGGCGGCGGGATGACCGCCGCGTTGACAGCTCACTC
GTGCGGGCTGGACACGCTGGTGGTCGAGAAGGCCGCGCACTTCGGCGGATCGACCGCGCTCTCGGGCGGCGGGATCTGGG
TGCCGGGTGCGCCGTCGCAGCGCAAGGCCGGCTACACACCCGACCCGGACGCGGTCTTCGGGTATCTCAAGCAGATCACC
GGGGGGCTGGTCAGCGATGCGCGCCTGCGCACCTACGTCGACGCCGCCCCGCAGATGATGGAGTTCCTCGAGAACCTCAG
CCCGTGGTTCGAATTCGTCTGGAAGCCCGGCTACGCCGACTACTACCCGGAACTGCCGGGCGGCTCCGAACTCGGCAGCA
CGATCAACGTGCCGGCCATCGACCTGCGCCGGCTCGAGGAGGACGAACAGCACCTGCTCCAGCCGTTGGCGCTGGCGCCG
AAGGGAATCTGGTTCGCGCCCAAGGACCTTCGCCTCTTCTATCAGGTGCGGCAGAACTGGCGCGGTAAGGCCGTCCTGGT
CAAGCTGATCTGGCGGATGGTGCGGGCGCGGGTGTTCGGCGACCGGATGGCCGCCATCGGCCAGTCCCTGGCCGCGCGGA
TGCGGCTGGCCATGAAGCAGGAGAACATCCCGCTGTGGCTCGACGCACCGATGACCGAGTTGATCACCGGTCCCGACGGC
GAGGTGGTCGGTGCGGTCGTCGAGACGGACGGGCGCGCGGTCCGGATCGGCGCGCGCCGCGGCGTCATCCTGGCCGCCGG
CGGCTTCGACCACGACATGGACTGGCGCAGAGAGCATCTGCCGGTTCTGGAGAAGGACTGGAGCTTCGGTAACCCGGCCA
ACCTGGGCGACGGTATCCGCGCGGCCGAGAAGGTCGGCGCCGCAACCGAACTGCTCGACGAGGCGTGGTGGTTCCCGGCG
ATGTGCTGGCCCGACGGGCGGCTGCAGTTCATGCTCAACGAACGCATGATGCCCGCGCAGTTCGTCGTCAACGGCGACGG
GAAGCGCTTCATCAACGAGGCCGCACCGTACATGGATTTCGCGCACGCGATGATCGACGGCCAGCGGTCCGGTGTGGAGC
ACATCCCGTGCTGGCTGATCACCGACATCCGGTCGTTCCACCGCTATGTGGTGGGCGGGCACCTGCCCATCCCGAAGGTG
CCCTTCGCGCCGGTGCCCACCGGGTGGAAGGTGCCCAAGGCCTGGCTGGATTCCGGGGTGGTCAAGGAAGCCCACTCGTG
GGACGAGATGGCCGCCAAGATCGGCGTGCCTGCCGAGAACCTGCGCCGGACCGCCGAACGGTTCAACGAGCTCGCGCGCA
AGGGACACGACGACGACTTCAACCGCGGCGACAGCGCGTACGACAACTACTACGGCGACCCGACGCTGCCGAACCCCAAC
CTTCACCCGCTGGGCAAGCCGCCGTACTACGCGTTCCAGATCATCCTCGGTGACCTCGGCACATCGGGCGGCCTGCGCAC
CGACGAGTACGCCCGGGTGCTGCGCGACGACGATTCGGCGGTGCCCGGCCTGTACGCGGTGGGCAACAACTCGGCCGCCG
TGATGGGCCGCAGCTACGCCGGTGCGGGCGCGACGATCGGGCCCGCCATGACGTTCGGCTACGTCGCCGCCAGACACATC
GCGGCGAAAGACGCCGCCGCGCAGCCGGATTCCGGCACCGCGCACGTCGACCACCAAGCTCAACCCAGTCCAGGGCCGTC
ACCCATCGGAGGAAGCACATGA

Upstream 100 bases:

>100_bases
CGGCGCTGGCGGCCGGCGGTGACTACCGCACCGGCCTGCGCTTCTACCGTGCCGCACCCGAGTTGATCGCGGGTTTCGCG
ATCCGGACGGCGGTGCTCGC

Downstream 100 bases:

>100_bases
AAATCTCGCTGTTCTACGAATTCCCGCTGCCGCGGCCGTGGTCGGAGGATGACGAGCACCAGTTGTTCCAGCACGGACTA
GACGAGGTCGAGGCCGCAGA

Product: 3-ketosteroid-delta-1-dehydrogenase

Products: NA

Alternate protein names: 3-keto-Delta(4)-steroid Delta(1)-dehydrogenase; KSDD; 3-oxo-Delta(4)-steroid 1-dehydrogenase; KSTD [H]

Number of amino acids: Translated: 593; Mature: 593

Protein sequence:

>593_residues
MNFDRHVDVLVVGSGGGGMTAALTAHSCGLDTLVVEKAAHFGGSTALSGGGIWVPGAPSQRKAGYTPDPDAVFGYLKQIT
GGLVSDARLRTYVDAAPQMMEFLENLSPWFEFVWKPGYADYYPELPGGSELGSTINVPAIDLRRLEEDEQHLLQPLALAP
KGIWFAPKDLRLFYQVRQNWRGKAVLVKLIWRMVRARVFGDRMAAIGQSLAARMRLAMKQENIPLWLDAPMTELITGPDG
EVVGAVVETDGRAVRIGARRGVILAAGGFDHDMDWRREHLPVLEKDWSFGNPANLGDGIRAAEKVGAATELLDEAWWFPA
MCWPDGRLQFMLNERMMPAQFVVNGDGKRFINEAAPYMDFAHAMIDGQRSGVEHIPCWLITDIRSFHRYVVGGHLPIPKV
PFAPVPTGWKVPKAWLDSGVVKEAHSWDEMAAKIGVPAENLRRTAERFNELARKGHDDDFNRGDSAYDNYYGDPTLPNPN
LHPLGKPPYYAFQIILGDLGTSGGLRTDEYARVLRDDDSAVPGLYAVGNNSAAVMGRSYAGAGATIGPAMTFGYVAARHI
AAKDAAAQPDSGTAHVDHQAQPSPGPSPIGGST

Sequences:

>Translated_593_residues
MNFDRHVDVLVVGSGGGGMTAALTAHSCGLDTLVVEKAAHFGGSTALSGGGIWVPGAPSQRKAGYTPDPDAVFGYLKQIT
GGLVSDARLRTYVDAAPQMMEFLENLSPWFEFVWKPGYADYYPELPGGSELGSTINVPAIDLRRLEEDEQHLLQPLALAP
KGIWFAPKDLRLFYQVRQNWRGKAVLVKLIWRMVRARVFGDRMAAIGQSLAARMRLAMKQENIPLWLDAPMTELITGPDG
EVVGAVVETDGRAVRIGARRGVILAAGGFDHDMDWRREHLPVLEKDWSFGNPANLGDGIRAAEKVGAATELLDEAWWFPA
MCWPDGRLQFMLNERMMPAQFVVNGDGKRFINEAAPYMDFAHAMIDGQRSGVEHIPCWLITDIRSFHRYVVGGHLPIPKV
PFAPVPTGWKVPKAWLDSGVVKEAHSWDEMAAKIGVPAENLRRTAERFNELARKGHDDDFNRGDSAYDNYYGDPTLPNPN
LHPLGKPPYYAFQIILGDLGTSGGLRTDEYARVLRDDDSAVPGLYAVGNNSAAVMGRSYAGAGATIGPAMTFGYVAARHI
AAKDAAAQPDSGTAHVDHQAQPSPGPSPIGGST
>Mature_593_residues
MNFDRHVDVLVVGSGGGGMTAALTAHSCGLDTLVVEKAAHFGGSTALSGGGIWVPGAPSQRKAGYTPDPDAVFGYLKQIT
GGLVSDARLRTYVDAAPQMMEFLENLSPWFEFVWKPGYADYYPELPGGSELGSTINVPAIDLRRLEEDEQHLLQPLALAP
KGIWFAPKDLRLFYQVRQNWRGKAVLVKLIWRMVRARVFGDRMAAIGQSLAARMRLAMKQENIPLWLDAPMTELITGPDG
EVVGAVVETDGRAVRIGARRGVILAAGGFDHDMDWRREHLPVLEKDWSFGNPANLGDGIRAAEKVGAATELLDEAWWFPA
MCWPDGRLQFMLNERMMPAQFVVNGDGKRFINEAAPYMDFAHAMIDGQRSGVEHIPCWLITDIRSFHRYVVGGHLPIPKV
PFAPVPTGWKVPKAWLDSGVVKEAHSWDEMAAKIGVPAENLRRTAERFNELARKGHDDDFNRGDSAYDNYYGDPTLPNPN
LHPLGKPPYYAFQIILGDLGTSGGLRTDEYARVLRDDDSAVPGLYAVGNNSAAVMGRSYAGAGATIGPAMTFGYVAARHI
AAKDAAAQPDSGTAHVDHQAQPSPGPSPIGGST

Specific function: Catalyzes the elimination of the C-1 and C-2 hydrogen atoms of the A-ring from the polycyclic ring structure of 3- ketosteroids. Is also involved in the formation of 3-keto-1,4- diene-steroid from 3-keto-4-ene-steroid [H]

COG id: COG1053

COG function: function code C; Succinate dehydrogenase/fumarate reductase, flavoprotein subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. 3-oxosteroid dehydrogenase subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =1.3.99.4 [H]

Molecular weight: Translated: 64577; Mature: 64577

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFDRHVDVLVVGSGGGGMTAALTAHSCGLDTLVVEKAAHFGGSTALSGGGIWVPGAPSQ
CCCCCEEEEEEEECCCCCCEEEEEHHHCCCHHHHHHHHHHCCCCCEECCCCEECCCCCCC
RKAGYTPDPDAVFGYLKQITGGLVSDARLRTYVDAAPQMMEFLENLSPWFEFVWKPGYAD
CCCCCCCCHHHHHHHHHHHHCCHHCCHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCCCC
YYPELPGGSELGSTINVPAIDLRRLEEDEQHLLQPLALAPKGIWFAPKDLRLFYQVRQNW
CCCCCCCCCCCCCCCCCCCHHHHHHCHHHHHHHHHHHHCCCCCEECCHHHHHHHHHHHCC
RGKAVLVKLIWRMVRARVFGDRMAAIGQSLAARMRLAMKQENIPLWLDAPMTELITGPDG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHCCCCC
EVVGAVVETDGRAVRIGARRGVILAAGGFDHDMDWRREHLPVLEKDWSFGNPANLGDGIR
CEEEEEEECCCCEEEEECCCCEEEEECCCCCCCCHHHHCCCCCCCCCCCCCCCCCCHHHH
AAEKVGAATELLDEAWWFPAMCWPDGRLQFMLNERMMPAQFVVNGDGKRFINEAAPYMDF
HHHHHHHHHHHHHHHCCCCEEECCCCCEEEEECCCCCCEEEEEECCCHHHHHHHHHHHHH
AHAMIDGQRSGVEHIPCWLITDIRSFHRYVVGGHLPIPKVPFAPVPTGWKVPKAWLDSGV
HHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHCCCH
VKEAHSWDEMAAKIGVPAENLRRTAERFNELARKGHDDDFNRGDSAYDNYYGDPTLPNPN
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCCCCCCC
LHPLGKPPYYAFQIILGDLGTSGGLRTDEYARVLRDDDSAVPGLYAVGNNSAAVMGRSYA
CCCCCCCCCEEEEEEEHHCCCCCCCCHHHHHHHHHCCCCCCCCEEEECCCCEEEEECCCC
GAGATIGPAMTFGYVAARHIAAKDAAAQPDSGTAHVDHQAQPSPGPSPIGGST
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNFDRHVDVLVVGSGGGGMTAALTAHSCGLDTLVVEKAAHFGGSTALSGGGIWVPGAPSQ
CCCCCEEEEEEEECCCCCCEEEEEHHHCCCHHHHHHHHHHCCCCCEECCCCEECCCCCCC
RKAGYTPDPDAVFGYLKQITGGLVSDARLRTYVDAAPQMMEFLENLSPWFEFVWKPGYAD
CCCCCCCCHHHHHHHHHHHHCCHHCCHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCCCC
YYPELPGGSELGSTINVPAIDLRRLEEDEQHLLQPLALAPKGIWFAPKDLRLFYQVRQNW
CCCCCCCCCCCCCCCCCCCHHHHHHCHHHHHHHHHHHHCCCCCEECCHHHHHHHHHHHCC
RGKAVLVKLIWRMVRARVFGDRMAAIGQSLAARMRLAMKQENIPLWLDAPMTELITGPDG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHCCCCC
EVVGAVVETDGRAVRIGARRGVILAAGGFDHDMDWRREHLPVLEKDWSFGNPANLGDGIR
CEEEEEEECCCCEEEEECCCCEEEEECCCCCCCCHHHHCCCCCCCCCCCCCCCCCCHHHH
AAEKVGAATELLDEAWWFPAMCWPDGRLQFMLNERMMPAQFVVNGDGKRFINEAAPYMDF
HHHHHHHHHHHHHHHCCCCEEECCCCCEEEEECCCCCCEEEEEECCCHHHHHHHHHHHHH
AHAMIDGQRSGVEHIPCWLITDIRSFHRYVVGGHLPIPKVPFAPVPTGWKVPKAWLDSGV
HHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHCCCH
VKEAHSWDEMAAKIGVPAENLRRTAERFNELARKGHDDDFNRGDSAYDNYYGDPTLPNPN
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCCCCCCC
LHPLGKPPYYAFQIILGDLGTSGGLRTDEYARVLRDDDSAVPGLYAVGNNSAAVMGRSYA
CCCCCCCCCEEEEEEEHHCCCCCCCCHHHHHHHHHCCCCCCCCEEEECCCCEEEEECCCC
GAGATIGPAMTFGYVAARHIAAKDAAAQPDSGTAHVDHQAQPSPGPSPIGGST
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12218036 [H]