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The map label for this gene is cysU [H]

Identifier: 108800447

GI number: 108800447

Start: 3708945

End: 3709790

Strand: Reverse

Name: cysU [H]

Synonym: Mmcs_3481

Alternate gene names: 108800447

Gene position: 3709790-3708945 (Counterclockwise)

Preceding gene: 108800448

Following gene: 108800446

Centisome position: 65.02

GC content: 69.39

Gene sequence:

>846_bases
ATGATCACTGCCGTCGACGGTGACAACGGCCGGGCGCCGGGCGGATTCGCCCGGCGTCGCGGTACCGCGTCCCTTCGGGT
CGGCGTCGCGACCATCTGGCTCAGCGTCATCGTGCTGCTGCCGCTGGCCGCGATCCTCTGGCAGTCCGTGGGCGGCGGGT
GGGAGGCGTTCTACCGCGCCGTGACCGGCAATGCCGCACTCGAGTCGTTCCGGGTGACCCTGACCATCTCGGTCGGGGTG
ACGATCGTCAACGCCTTCTTCGGCCTGCTGGTGGCCTGGGTGCTCACCCGCGACGACTTTCCGGGCCGACGGCTGGTGGA
CGCCGTCATCGACCTGCCCTTCGCGCTGCCCACGATCGTCGCCAGCTTGGTCATGCTGGCGCTCTACGGACCGGCGAGCC
CGGTCCAGCTGCACCTGCAGCACACCAAGTGGGGTGTGGGAATCGCGCTGCTGTTCGTCACGCTGCCGTTCGTTGTGCGC
TCGGTGCAGCCGGTGTTGCTCGAACTGGACCGGGAGGTCGAAGAGGCGGCCGCCTCGCTGGGCGCCAACAACTGGACCAT
ACTGACCAGGATCATCCTGCCCGCACTGCTCCCGTCGCTGCTGTCCGGCGCCGGGCTGGCGTTCTCCCGCGCCATCGGCG
AGTTCGGCTCGGTGGTGCTCATCGGTGGCGCGGTGCCGGGGGAGACCGAGGTGTCCTCGCAGTGGATCCGCACGCTGATC
GAGAACGACGACCGCACCGGTGCGGCCGCCATCTCGATCGTGCTGCTGGCGATCTCGTTCGTCGTGCTGTTGATCCTGCG
CGCGCTCGGCGCGCGGGCGGCCAAACGGGAACAGATGGCGGGGTGA

Upstream 100 bases:

>100_bases
TGTGGACCATCGCCGACCTGGGTGGCTGGCAGAAGGTGGACAGCGAGCTGTTCAACAAGGACTCCGGTTCGATCACCAAG
ATCTACACCCAGGCGACTGG

Downstream 100 bases:

>100_bases
TCGAGCAATGCTGTTGTCGCCGGTGACGCGCCGGGTACTGCGCTTCGTCGCGCTGGCCTACATCCTGGTGCTGGTCGTCG
TGCCGGTGGGGCTGATCCTG

Product: Sulfate ABC transporter permease CysT

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MITAVDGDNGRAPGGFARRRGTASLRVGVATIWLSVIVLLPLAAILWQSVGGGWEAFYRAVTGNAALESFRVTLTISVGV
TIVNAFFGLLVAWVLTRDDFPGRRLVDAVIDLPFALPTIVASLVMLALYGPASPVQLHLQHTKWGVGIALLFVTLPFVVR
SVQPVLLELDREVEEAAASLGANNWTILTRIILPALLPSLLSGAGLAFSRAIGEFGSVVLIGGAVPGETEVSSQWIRTLI
ENDDRTGAAAISIVLLAISFVVLLILRALGARAAKREQMAG

Sequences:

>Translated_281_residues
MITAVDGDNGRAPGGFARRRGTASLRVGVATIWLSVIVLLPLAAILWQSVGGGWEAFYRAVTGNAALESFRVTLTISVGV
TIVNAFFGLLVAWVLTRDDFPGRRLVDAVIDLPFALPTIVASLVMLALYGPASPVQLHLQHTKWGVGIALLFVTLPFVVR
SVQPVLLELDREVEEAAASLGANNWTILTRIILPALLPSLLSGAGLAFSRAIGEFGSVVLIGGAVPGETEVSSQWIRTLI
ENDDRTGAAAISIVLLAISFVVLLILRALGARAAKREQMAG
>Mature_281_residues
MITAVDGDNGRAPGGFARRRGTASLRVGVATIWLSVIVLLPLAAILWQSVGGGWEAFYRAVTGNAALESFRVTLTISVGV
TIVNAFFGLLVAWVLTRDDFPGRRLVDAVIDLPFALPTIVASLVMLALYGPASPVQLHLQHTKWGVGIALLFVTLPFVVR
SVQPVLLELDREVEEAAASLGANNWTILTRIILPALLPSLLSGAGLAFSRAIGEFGSVVLIGGAVPGETEVSSQWIRTLI
ENDDRTGAAAISIVLLAISFVVLLILRALGARAAKREQMAG

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0555

COG function: function code O; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788764, Length=231, Percent_Identity=41.1255411255411, Blast_Score=171, Evalue=4e-44,
Organism=Escherichia coli, GI87082099, Length=261, Percent_Identity=32.183908045977, Blast_Score=131, Evalue=5e-32,
Organism=Escherichia coli, GI1786980, Length=203, Percent_Identity=32.512315270936, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI1787368, Length=233, Percent_Identity=27.0386266094421, Blast_Score=64, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011865
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 29724; Mature: 29724

Theoretical pI: Translated: 9.12; Mature: 9.12

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITAVDGDNGRAPGGFARRRGTASLRVGVATIWLSVIVLLPLAAILWQSVGGGWEAFYRA
CEEEECCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
VTGNAALESFRVTLTISVGVTIVNAFFGLLVAWVLTRDDFPGRRLVDAVIDLPFALPTIV
HHCHHHHHHEEEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCHHHHHHHH
ASLVMLALYGPASPVQLHLQHTKWGVGIALLFVTLPFVVRSVQPVLLELDREVEEAAASL
HHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GANNWTILTRIILPALLPSLLSGAGLAFSRAIGEFGSVVLIGGAVPGETEVSSQWIRTLI
CCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHH
ENDDRTGAAAISIVLLAISFVVLLILRALGARAAKREQMAG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCC
>Mature Secondary Structure
MITAVDGDNGRAPGGFARRRGTASLRVGVATIWLSVIVLLPLAAILWQSVGGGWEAFYRA
CEEEECCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
VTGNAALESFRVTLTISVGVTIVNAFFGLLVAWVLTRDDFPGRRLVDAVIDLPFALPTIV
HHCHHHHHHEEEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCHHHHHHHH
ASLVMLALYGPASPVQLHLQHTKWGVGIALLFVTLPFVVRSVQPVLLELDREVEEAAASL
HHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GANNWTILTRIILPALLPSLLSGAGLAFSRAIGEFGSVVLIGGAVPGETEVSSQWIRTLI
CCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHH
ENDDRTGAAAISIVLLAISFVVLLILRALGARAAKREQMAG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2188958; 9205837; 9278503; 2188959 [H]