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The map label for this gene is sbpA [H]

Identifier: 108800448

GI number: 108800448

Start: 3709787

End: 3710824

Strand: Reverse

Name: sbpA [H]

Synonym: Mmcs_3482

Alternate gene names: 108800448

Gene position: 3710824-3709787 (Counterclockwise)

Preceding gene: 108800449

Following gene: 108800447

Centisome position: 65.04

GC content: 67.44

Gene sequence:

>1038_bases
ATGCTCAGCTCAGTCAAGACCTTCAAGCGCTGGCGGGTCGCCGCGGCGCTCGCCGCCACCGCGTCACTTCTGGCCGGGTG
CGGTGGCGGCGCCAGCGACGTCGCGGGCTCCGACGGTGGCTCCAACGCCGAGACCACCCTGACCCTGGTCGCCTACGCGG
TGCCCGAACCCGGGTGGTCCAAGGTCATCCCGGCGTTCGCGGCCACCGAGGAGGGTAAGGGTGTCGGCGTGACCGCATCC
TACGGCGCCTCCGGCGATCAGTCCCGCGGCGTCGACTCGGGTAAACCCGCCGACGTGGTGAACTTCTCGGTCGAACCCGA
CATCACCCGCCTGGTGAAGTCCGGCAAGGTCAGCGAGGACTGGAACAAGAACGCCACCAAGGGGATTCCGTTCGGTTCGG
TGGTGAGCCTGATGGTCCGCCCGGGCAACCCGAAGAACATCCGCGACTGGGACGACCTGCTCAAGCCGGGCGTCGAGGTC
ATCACCCCCAGCCCGCTCAGCTCGGGTTCGGCCAAGTGGAACCTGCTCGCCCCGTACGCGGTGAAGAGCGACGGGGGCAA
GAACCCGCAGGCCGGTCTCGACTACGTCAACCAGCTGGTCAGCGAGCACATCAAGCTGCGGCCCGGCTCCGGGCGCGAGG
CCACCGACGTCTTCCTGCAGGGCAGCGGCGACGTACTGCTGGCATATGAGAACGAGGCGATCAACTCGCGCCGCCTCGGC
CACGAGTTCGACTACTTCAACCCTCCGCAGACGTTCAAGATCGAGAACCCGGTCGCGGTGGTCAACTCCAGCCGGCATCT
CGAGCAGGCGACGGCGTTCGTGAACTTCCAGTACACGCCCGAGGCGCAGAAGCTGTGGGCCGAGGCCGGGTTCCGCCCGG
TCGACCCGGCGGTCGCCGCGGAGTTCGCCGACCAGTTCCCGGCGCCGCAGAAGCTGTGGACCATCGCCGACCTGGGTGGC
TGGCAGAAGGTGGACAGCGAGCTGTTCAACAAGGACTCCGGTTCGATCACCAAGATCTACACCCAGGCGACTGGATGA

Upstream 100 bases:

>100_bases
CTTCGGCGACGGCATTTTTCGTGCTGCCTTCTCGTCGTCACCAGTGTCCGCTTGACGGGGTCCGTCCTGCATTCCACGGA
AGACGATCGAAAGGCCATCG

Downstream 100 bases:

>100_bases
TCACTGCCGTCGACGGTGACAACGGCCGGGCGCCGGGCGGATTCGCCCGGCGTCGCGGTACCGCGTCCCTTCGGGTCGGC
GTCGCGACCATCTGGCTCAG

Product: thiosulfate-binding protein

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MLSSVKTFKRWRVAAALAATASLLAGCGGGASDVAGSDGGSNAETTLTLVAYAVPEPGWSKVIPAFAATEEGKGVGVTAS
YGASGDQSRGVDSGKPADVVNFSVEPDITRLVKSGKVSEDWNKNATKGIPFGSVVSLMVRPGNPKNIRDWDDLLKPGVEV
ITPSPLSSGSAKWNLLAPYAVKSDGGKNPQAGLDYVNQLVSEHIKLRPGSGREATDVFLQGSGDVLLAYENEAINSRRLG
HEFDYFNPPQTFKIENPVAVVNSSRHLEQATAFVNFQYTPEAQKLWAEAGFRPVDPAVAAEFADQFPAPQKLWTIADLGG
WQKVDSELFNKDSGSITKIYTQATG

Sequences:

>Translated_345_residues
MLSSVKTFKRWRVAAALAATASLLAGCGGGASDVAGSDGGSNAETTLTLVAYAVPEPGWSKVIPAFAATEEGKGVGVTAS
YGASGDQSRGVDSGKPADVVNFSVEPDITRLVKSGKVSEDWNKNATKGIPFGSVVSLMVRPGNPKNIRDWDDLLKPGVEV
ITPSPLSSGSAKWNLLAPYAVKSDGGKNPQAGLDYVNQLVSEHIKLRPGSGREATDVFLQGSGDVLLAYENEAINSRRLG
HEFDYFNPPQTFKIENPVAVVNSSRHLEQATAFVNFQYTPEAQKLWAEAGFRPVDPAVAAEFADQFPAPQKLWTIADLGG
WQKVDSELFNKDSGSITKIYTQATG
>Mature_345_residues
MLSSVKTFKRWRVAAALAATASLLAGCGGGASDVAGSDGGSNAETTLTLVAYAVPEPGWSKVIPAFAATEEGKGVGVTAS
YGASGDQSRGVDSGKPADVVNFSVEPDITRLVKSGKVSEDWNKNATKGIPFGSVVSLMVRPGNPKNIRDWDDLLKPGVEV
ITPSPLSSGSAKWNLLAPYAVKSDGGKNPQAGLDYVNQLVSEHIKLRPGSGREATDVFLQGSGDVLLAYENEAINSRRLG
HEFDYFNPPQTFKIENPVAVVNSSRHLEQATAFVNFQYTPEAQKLWAEAGFRPVDPAVAAEFADQFPAPQKLWTIADLGG
WQKVDSELFNKDSGSITKIYTQATG

Specific function: This protein specifically binds sulfate and is involved in its transmembrane transport [H]

COG id: COG1613

COG function: function code P; ABC-type sulfate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic sulfate-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1790351, Length=281, Percent_Identity=35.9430604982206, Blast_Score=162, Evalue=3e-41,
Organism=Escherichia coli, GI1788765, Length=300, Percent_Identity=30, Blast_Score=121, Evalue=6e-29,

Paralogues:

None

Copy number: 1240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR000957
- InterPro:   IPR006311
- InterPro:   IPR005669 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 36633; Mature: 36633

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSSVKTFKRWRVAAALAATASLLAGCGGGASDVAGSDGGSNAETTLTLVAYAVPEPGWS
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCHH
KVIPAFAATEEGKGVGVTASYGASGDQSRGVDSGKPADVVNFSVEPDITRLVKSGKVSED
HHCCHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHCCCCCCC
WNKNATKGIPFGSVVSLMVRPGNPKNIRDWDDLLKPGVEVITPSPLSSGSAKWNLLAPYA
CCCCCCCCCCHHHHHHEEECCCCCCCCCCHHHHHHCCCEEECCCCCCCCCCCEEEEECEE
VKSDGGKNPQAGLDYVNQLVSEHIKLRPGSGREATDVFLQGSGDVLLAYENEAINSRRLG
EECCCCCCCHHHHHHHHHHHHHHEEECCCCCCCCEEEEEECCCCEEEEECCCCCCHHHCC
HEFDYFNPPQTFKIENPVAVVNSSRHLEQATAFVNFQYTPEAQKLWAEAGFRPVDPAVAA
CCCCCCCCCCCEEECCCEEEECCCHHHHHHEEEEEEEECCHHHHHHHHCCCCCCCHHHHH
EFADQFPAPQKLWTIADLGGWQKVDSELFNKDSGSITKIYTQATG
HHHHHCCCCHHEEEECCCCCHHHHHHHHHCCCCCCEEEEEEECCC
>Mature Secondary Structure
MLSSVKTFKRWRVAAALAATASLLAGCGGGASDVAGSDGGSNAETTLTLVAYAVPEPGWS
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCHH
KVIPAFAATEEGKGVGVTASYGASGDQSRGVDSGKPADVVNFSVEPDITRLVKSGKVSED
HHCCHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHCCCCCCC
WNKNATKGIPFGSVVSLMVRPGNPKNIRDWDDLLKPGVEVITPSPLSSGSAKWNLLAPYA
CCCCCCCCCCHHHHHHEEECCCCCCCCCCHHHHHHCCCEEECCCCCCCCCCCEEEEECEE
VKSDGGKNPQAGLDYVNQLVSEHIKLRPGSGREATDVFLQGSGDVLLAYENEAINSRRLG
EECCCCCCCHHHHHHHHHHHHHHEEECCCCCCCCEEEEEECCCCEEEEECCCCCCHHHCC
HEFDYFNPPQTFKIENPVAVVNSSRHLEQATAFVNFQYTPEAQKLWAEAGFRPVDPAVAA
CCCCCCCCCCCEEECCCEEEECCCHHHHHHEEEEEEEECCHHHHHHHHCCCCCCCHHHHH
EFADQFPAPQKLWTIADLGGWQKVDSELFNKDSGSITKIYTQATG
HHHHHCCCCHHEEEECCCCCHHHHHHHHHCCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1708375 [H]