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The map label for this gene is yciL [C]

Identifier: 108799895

GI number: 108799895

Start: 3086021

End: 3086764

Strand: Reverse

Name: yciL [C]

Synonym: Mmcs_2929

Alternate gene names: 108799895

Gene position: 3086764-3086021 (Counterclockwise)

Preceding gene: 108799896

Following gene: 108799894

Centisome position: 54.1

GC content: 67.74

Gene sequence:

>744_bases
ATGACCGAACCCGATGGTGTCCGACTGCAGAAAGTGTTGTCGCAGGCCGGAATCGCGTCCCGCCGAGTCGCCGAGAAGAT
GATCCTCGACGGCCGCGTCGAAGTCGACGGCCACATCGTCACCGAACTCGGCACCCGGGTCGATCCCACCGCGGCCGACA
TCCGCGTCGACGGCACCAGGGTCCTGCTCGACGACACCCTCGTGCACCTCGCCATCAACAAGCCGCGCGGGATGCACTCC
ACGATGTCCGACGACCGCGGACGGCCCTGTATCGGTGACCTCGTCGAGCACCGGGTGCGCGGCAACAAGGGGTTGTTCCA
CGTCGGCCGCCTCGACGCGGACACCGAGGGTCTGATGCTGCTGACCAACGACGGCGAACTCGCACACCGGTTGATGCACC
CGTCCTACGAGGTGCCCAAGACCTACGTCGCCACCGTGCTGGGAACCGTCCCGCGCGGGCTGGGCAAGAAGCTGCGCGCC
GGAGTGGAACTGGACGACGGACCCGCCCGAGTGGACGACTTCGCGGTCGTGGACGCGATGCCCGGCCGGTCGATGGTCCG
GGTGACCCTGCACGAGGGGCGCAAGCGTATCGTTCGTCGCTTGTTGGCGGCGGTCGGATTCCCGGTGCAGGAGCTGGTGC
GCACCGACATCGGGGCGGTCTCGCTCGGCGAGCAGCGCCCCGGCAGCATTCGGGTGTTGACCCGCAAGGAGATCGGTGAA
CTGTACAAGGCGGTGGGGATGTGA

Upstream 100 bases:

>100_bases
TGAGCGAATCGCTCGCGGACGAACCGAGATTCATGAAACTGGGCGGCGTGCCCGCACCCACCGGACAGCCGATGTCCTAC
GACGTGGACAAGGATGACCG

Downstream 100 bases:

>100_bases
CTGACGGAACGGTGATCGCCGTCGACGGCCCCGCCGGCACCGGAAAGTCCTCGGTGTCAAGGGGTTTGGCTCGCGCGCTG
GGCGCCCGGTATCTCGACAC

Product: ribosomal large subunit pseudouridine synthase B

Products: NA

Alternate protein names: RNA pseudouridylate synthase; RNA-uridine isomerase [H]

Number of amino acids: Translated: 247; Mature: 246

Protein sequence:

>247_residues
MTEPDGVRLQKVLSQAGIASRRVAEKMILDGRVEVDGHIVTELGTRVDPTAADIRVDGTRVLLDDTLVHLAINKPRGMHS
TMSDDRGRPCIGDLVEHRVRGNKGLFHVGRLDADTEGLMLLTNDGELAHRLMHPSYEVPKTYVATVLGTVPRGLGKKLRA
GVELDDGPARVDDFAVVDAMPGRSMVRVTLHEGRKRIVRRLLAAVGFPVQELVRTDIGAVSLGEQRPGSIRVLTRKEIGE
LYKAVGM

Sequences:

>Translated_247_residues
MTEPDGVRLQKVLSQAGIASRRVAEKMILDGRVEVDGHIVTELGTRVDPTAADIRVDGTRVLLDDTLVHLAINKPRGMHS
TMSDDRGRPCIGDLVEHRVRGNKGLFHVGRLDADTEGLMLLTNDGELAHRLMHPSYEVPKTYVATVLGTVPRGLGKKLRA
GVELDDGPARVDDFAVVDAMPGRSMVRVTLHEGRKRIVRRLLAAVGFPVQELVRTDIGAVSLGEQRPGSIRVLTRKEIGE
LYKAVGM
>Mature_246_residues
TEPDGVRLQKVLSQAGIASRRVAEKMILDGRVEVDGHIVTELGTRVDPTAADIRVDGTRVLLDDTLVHLAINKPRGMHST
MSDDRGRPCIGDLVEHRVRGNKGLFHVGRLDADTEGLMLLTNDGELAHRLMHPSYEVPKTYVATVLGTVPRGLGKKLRAG
VELDDGPARVDDFAVVDAMPGRSMVRVTLHEGRKRIVRRLLAAVGFPVQELVRTDIGAVSLGEQRPGSIRVLTRKEIGEL
YKAVGM

Specific function: Unknown

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787524, Length=220, Percent_Identity=43.1818181818182, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1788510, Length=236, Percent_Identity=33.4745762711864, Blast_Score=107, Evalue=6e-25,
Organism=Escherichia coli, GI1790453, Length=245, Percent_Identity=34.2857142857143, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI87081838, Length=192, Percent_Identity=33.8541666666667, Blast_Score=92, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 26985; Mature: 26854

Theoretical pI: Translated: 9.54; Mature: 9.54

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEPDGVRLQKVLSQAGIASRRVAEKMILDGRVEVDGHIVTELGTRVDPTAADIRVDGTR
CCCCCCHHHHHHHHHHCHHHHHHHHHHHHCCEEEECCEEHHHHCCCCCCCCCEEEECCEE
VLLDDTLVHLAINKPRGMHSTMSDDRGRPCIGDLVEHRVRGNKGLFHVGRLDADTEGLML
EEEECEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCEEE
LTNDGELAHRLMHPSYEVPKTYVATVLGTVPRGLGKKLRAGVELDDGPARVDDFAVVDAM
EECCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECC
PGRSMVRVTLHEGRKRIVRRLLAAVGFPVQELVRTDIGAVSLGEQRPGSIRVLTRKEIGE
CCCCEEEEEHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCEECCCCCCCCEEEEEHHHHHH
LYKAVGM
HHHHHCC
>Mature Secondary Structure 
TEPDGVRLQKVLSQAGIASRRVAEKMILDGRVEVDGHIVTELGTRVDPTAADIRVDGTR
CCCCCHHHHHHHHHHCHHHHHHHHHHHHCCEEEECCEEHHHHCCCCCCCCCEEEECCEE
VLLDDTLVHLAINKPRGMHSTMSDDRGRPCIGDLVEHRVRGNKGLFHVGRLDADTEGLML
EEEECEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCEEE
LTNDGELAHRLMHPSYEVPKTYVATVLGTVPRGLGKKLRAGVELDDGPARVDDFAVVDAM
EECCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECC
PGRSMVRVTLHEGRKRIVRRLLAAVGFPVQELVRTDIGAVSLGEQRPGSIRVLTRKEIGE
CCCCEEEEEHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCEECCCCCCCCEEEEEHHHHHH
LYKAVGM
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]