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The map label for this gene is sigW [H]

Identifier: 108797119

GI number: 108797119

Start: 156564

End: 157577

Strand: Reverse

Name: sigW [H]

Synonym: Mmcs_0139

Alternate gene names: 108797119

Gene position: 157577-156564 (Counterclockwise)

Preceding gene: 108797126

Following gene: 108797117

Centisome position: 2.76

GC content: 69.72

Gene sequence:

>1014_bases
ATGTCGGCCATCGTGCGTAAGCTCGCCAGCGTGACCGTCCTGGCTCGTACCGTCGACGACGGCCCTGACGAAGACGCATT
CCTCGCCGATGCACAGAAGTATCGACGGGAACTGCTCGCGCACTGCTACCGGATGACCGGGTCACTGCACGACGCCGAAG
ACCTGGTGCAGGAGACGTATCTGCGGGCGTGGAAGTCCTACAAGGGTTTCCAGGGCAAGTCGTCGGTGCGCACCTGGCTC
TACCGGATCGCCACGAACACCTCGCTGACCGCGCTCGACGGCCGGGCTCGCCGGCCGTTGCCCACCGGCCTCGGTGCGCC
CAGTTCCGACCCGGTCGACGACATCCACGAACGCGCCGAGGTGCCGTGGCTGCAGCCGCTGCCCGACGACGCCGGCGACC
CGTCGAGCATCGTGGGCTCACGGGAGTCGGTGCGGTTGGCGTTCATCGCGGCGCTGCAGCACCTCTCCCCGCGGCAGCGC
GCGGTGCTGGTGTTGCGCGAGGTGCTGCAGTGGAAGGCCGCCGAGGTCGCCGACGCGCTGGGCACGTCCACCGCGGCGGT
GAACAGCCTGCTGCAGCGGGCGCGGGCCCAACTCGACGCCATCGGGCCGAGCGAGGACGACGACCTGCGCCCCCCGGAGT
CTCCGGAGGCCAGGGACCTGCTGACCCGCTACATCGCGGCGTTCGAGACCTACGACGTCGACAGGCTGGCCGACCTGTTC
ACCGCGGAGGCCGTGTGGGAGATGCCGCCGTTCGACGGGTGGTACCAGGGCCCGCACGACATCGTGCTGCTGTCGAAGAC
GCACTGCCCGGCCGAACGGGCAGGCGATATGCAGCTGATTCCGACCACCGCCAACGGGCAGACCGCCGCGGCGCTGTACA
TGCGCAATCCGGCGACCGGTCGGCACGAGGCCTTCCAGCTGCATGTCCTCGACGTCACCGCGGACGGCATATCGCACGTG
GTGGCGTTCCACGCGACGACGTTCGCGCCGTTCGGGCTGGCGGACACGCTGTAG

Upstream 100 bases:

>100_bases
ACGATCCTCACCCCGCCGACGCCGTCGAAATGTCGCTCGCTGCGGGTGCTGGCCATGGCTCGAAAGACTATCGGTCGCGG
CCGGGACAACGGGGTGCCGG

Downstream 100 bases:

>100_bases
GTCGCCGCTGCTGCGCCGAGATCAGGGTTACGCCCAGCGTTCGACCGAAATGCGTGGGTCAACTCTGACCTGGGCCCAGT
TCGGCCAACCGGCCGCCCGC

Product: RNA polymerase factor sigma-70

Products: NA

Alternate protein names: Sigma-W factor [H]

Number of amino acids: Translated: 337; Mature: 336

Protein sequence:

>337_residues
MSAIVRKLASVTVLARTVDDGPDEDAFLADAQKYRRELLAHCYRMTGSLHDAEDLVQETYLRAWKSYKGFQGKSSVRTWL
YRIATNTSLTALDGRARRPLPTGLGAPSSDPVDDIHERAEVPWLQPLPDDAGDPSSIVGSRESVRLAFIAALQHLSPRQR
AVLVLREVLQWKAAEVADALGTSTAAVNSLLQRARAQLDAIGPSEDDDLRPPESPEARDLLTRYIAAFETYDVDRLADLF
TAEAVWEMPPFDGWYQGPHDIVLLSKTHCPAERAGDMQLIPTTANGQTAAALYMRNPATGRHEAFQLHVLDVTADGISHV
VAFHATTFAPFGLADTL

Sequences:

>Translated_337_residues
MSAIVRKLASVTVLARTVDDGPDEDAFLADAQKYRRELLAHCYRMTGSLHDAEDLVQETYLRAWKSYKGFQGKSSVRTWL
YRIATNTSLTALDGRARRPLPTGLGAPSSDPVDDIHERAEVPWLQPLPDDAGDPSSIVGSRESVRLAFIAALQHLSPRQR
AVLVLREVLQWKAAEVADALGTSTAAVNSLLQRARAQLDAIGPSEDDDLRPPESPEARDLLTRYIAAFETYDVDRLADLF
TAEAVWEMPPFDGWYQGPHDIVLLSKTHCPAERAGDMQLIPTTANGQTAAALYMRNPATGRHEAFQLHVLDVTADGISHV
VAFHATTFAPFGLADTL
>Mature_336_residues
SAIVRKLASVTVLARTVDDGPDEDAFLADAQKYRRELLAHCYRMTGSLHDAEDLVQETYLRAWKSYKGFQGKSSVRTWLY
RIATNTSLTALDGRARRPLPTGLGAPSSDPVDDIHERAEVPWLQPLPDDAGDPSSIVGSRESVRLAFIAALQHLSPRQRA
VLVLREVLQWKAAEVADALGTSTAAVNSLLQRARAQLDAIGPSEDDDLRPPESPEARDLLTRYIAAFETYDVDRLADLFT
AEAVWEMPPFDGWYQGPHDIVLLSKTHCPAERAGDMQLIPTTANGQTAAALYMRNPATGRHEAFQLHVLDVTADGISHVV
AFHATTFAPFGLADTL

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. Sigma-W controls genes involved in transport processes and detoxification [H]

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=187, Percent_Identity=31.0160427807487, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014294
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 37009; Mature: 36878

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS01063 SIGMA70_ECF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAIVRKLASVTVLARTVDDGPDEDAFLADAQKYRRELLAHCYRMTGSLHDAEDLVQETY
CHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
LRAWKSYKGFQGKSSVRTWLYRIATNTSLTALDGRARRPLPTGLGAPSSDPVDDIHERAE
HHHHHHHCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHC
VPWLQPLPDDAGDPSSIVGSRESVRLAFIAALQHLSPRQRAVLVLREVLQWKAAEVADAL
CCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
GTSTAAVNSLLQRARAQLDAIGPSEDDDLRPPESPEARDLLTRYIAAFETYDVDRLADLF
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHH
TAEAVWEMPPFDGWYQGPHDIVLLSKTHCPAERAGDMQLIPTTANGQTAAALYMRNPATG
HHHHHHCCCCCCCCCCCCCCEEEEECCCCCHHHCCCEEEEEECCCCCEEEEEEEECCCCC
RHEAFQLHVLDVTADGISHVVAFHATTFAPFGLADTL
CCCEEEEEEEEECHHHHHHHHHHHHHHCCCCCHHCCC
>Mature Secondary Structure 
SAIVRKLASVTVLARTVDDGPDEDAFLADAQKYRRELLAHCYRMTGSLHDAEDLVQETY
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
LRAWKSYKGFQGKSSVRTWLYRIATNTSLTALDGRARRPLPTGLGAPSSDPVDDIHERAE
HHHHHHHCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHC
VPWLQPLPDDAGDPSSIVGSRESVRLAFIAALQHLSPRQRAVLVLREVLQWKAAEVADAL
CCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
GTSTAAVNSLLQRARAQLDAIGPSEDDDLRPPESPEARDLLTRYIAAFETYDVDRLADLF
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHH
TAEAVWEMPPFDGWYQGPHDIVLLSKTHCPAERAGDMQLIPTTANGQTAAALYMRNPATG
HHHHHHCCCCCCCCCCCCCCEEEEECCCCCHHHCCCEEEEEECCCCCEEEEEEEECCCCC
RHEAFQLHVLDVTADGISHVVAFHATTFAPFGLADTL
CCCEEEEEEEEECHHHHHHHHHHHHHHCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9274029; 9384377; 12207695 [H]