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The map label for this gene is lacC2 [H]

Identifier: 108797062

GI number: 108797062

Start: 94670

End: 95647

Strand: Reverse

Name: lacC2 [H]

Synonym: Mmcs_0081

Alternate gene names: 108797062

Gene position: 95647-94670 (Counterclockwise)

Preceding gene: 108797063

Following gene: 108797061

Centisome position: 1.68

GC content: 75.97

Gene sequence:

>978_bases
ATGATCGTCACCGTCACCCCCAACCCCAGCATCGACCGGACGGTCGCGCTGCCGGCCCCGCTGACCCGCGGTGCGGTGCA
GCGGGTCACGTCGGTCACCAGCGAGCCGGGCGGTAAGGGCGTCAACGTCGCCCGGGCCCTGACCCTCGCGCGGCTGGACG
CGGTCGCGCTGCTGCCCGCCGCGGACGGCGACCCGATGGTCACCGCACTGCGCGCGATCGGTGTTCCGTTCCGCTGTGTG
CCGATCGCCGAACCTGTCCGCACCAACCTGGCGATCACCGAATCCGACGGAACCACAACGAAACTCAATGAGCGCGGCGC
GGCGCTGGACGCCGCCGCCCGAGACGCGCTGACCCGGGGCATCATCGACGCCGCCGAGCAGGCCAGCTGGGTGGTGCTGT
CCGGCTCACTGCCGCCCGGCCTGCCCGACGACTGGTACGCCGAGGTGGTCGCGCGGCTGGTCCCCCTGCGCTGCCGGGTC
GCCGTCGACACCTCCGAACGCCCGCTCGCCGCGCTCGCCGCCGCCTTCGACCATGCGGCGCCCGACGTCATCAAGCCGAA
CGCCGAGGAACTGGCCGGCCTCGTCGGCGCATCGGCCGAGGAGCTCGAAAGCGCCGCCGCACAGGGTGATCCGGAACCGG
TCGCGGCCGCCGCCCGCCAGCTCGTCGACCGCGGCGTCGGCGCGGTGCTCGCCACGCTGGGCGCCGCGGGCGCCGTCCTC
GTCGACCGGACCGGCGCGTGGATGGCGACGCCGCCGCCCATCGTCCCGCGCAGCACCGTCGGTGCGGGCGACGCGTCGCT
GGCCGGCTACGTCCGTGCGGCCGTGGCCGGCGCCGAGCCGCCCCGCCGGCTGCGCATGGCGGTCGCCTACGGCAGCGCCG
CCGCGGCCCTACCCGGCTCCGCACTGCCGTCCCCCGCCCAGCTCGACCTCGACGGCGTCGCCGTCAGCACCATCGCCCCG
ACCCCTGCCAGCTCGTGA

Upstream 100 bases:

>100_bases
TCGCGCCGATCAGCAGCGTCGACACCCTGATCACCGACGGCGAGATCAGCGACAACTACCGCGGGCAGTTCGCCGAACGC
GGCGTCGAGGTCGTGGTCGC

Downstream 100 bases:

>100_bases
ACCGACCGAAAGAGCCCGTGATGTCCAGTCCCACCGCATCGCCGATCATCACCACCGATCTCGTGCTGCTCGACGTCGAC
GCCGGCGGCGACAAGGAAGC

Product: PfkB

Products: NA

Alternate protein names: Phosphotagatokinase 2 [H]

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MIVTVTPNPSIDRTVALPAPLTRGAVQRVTSVTSEPGGKGVNVARALTLARLDAVALLPAADGDPMVTALRAIGVPFRCV
PIAEPVRTNLAITESDGTTTKLNERGAALDAAARDALTRGIIDAAEQASWVVLSGSLPPGLPDDWYAEVVARLVPLRCRV
AVDTSERPLAALAAAFDHAAPDVIKPNAEELAGLVGASAEELESAAAQGDPEPVAAAARQLVDRGVGAVLATLGAAGAVL
VDRTGAWMATPPPIVPRSTVGAGDASLAGYVRAAVAGAEPPRRLRMAVAYGSAAAALPGSALPSPAQLDLDGVAVSTIAP
TPASS

Sequences:

>Translated_325_residues
MIVTVTPNPSIDRTVALPAPLTRGAVQRVTSVTSEPGGKGVNVARALTLARLDAVALLPAADGDPMVTALRAIGVPFRCV
PIAEPVRTNLAITESDGTTTKLNERGAALDAAARDALTRGIIDAAEQASWVVLSGSLPPGLPDDWYAEVVARLVPLRCRV
AVDTSERPLAALAAAFDHAAPDVIKPNAEELAGLVGASAEELESAAAQGDPEPVAAAARQLVDRGVGAVLATLGAAGAVL
VDRTGAWMATPPPIVPRSTVGAGDASLAGYVRAAVAGAEPPRRLRMAVAYGSAAAALPGSALPSPAQLDLDGVAVSTIAP
TPASS
>Mature_325_residues
MIVTVTPNPSIDRTVALPAPLTRGAVQRVTSVTSEPGGKGVNVARALTLARLDAVALLPAADGDPMVTALRAIGVPFRCV
PIAEPVRTNLAITESDGTTTKLNERGAALDAAARDALTRGIIDAAEQASWVVLSGSLPPGLPDDWYAEVVARLVPLRCRV
AVDTSERPLAALAAAFDHAAPDVIKPNAEELAGLVGASAEELESAAAQGDPEPVAAAARQLVDRGVGAVLATLGAAGAVL
VDRTGAWMATPPPIVPRSTVGAGDASLAGYVRAAVAGAEPPRRLRMAVAYGSAAAALPGSALPSPAQLDLDGVAVSTIAP
TPASS

Specific function: Key control step of glycolysis. [C]

COG id: COG1105

COG function: function code G; Fructose-1-phosphate kinase and related fructose-6-phosphate kinase (PfkB)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carbohydrate kinase pfkB family. LacC subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788017, Length=307, Percent_Identity=32.2475570032573, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI1788493, Length=286, Percent_Identity=29.7202797202797, Blast_Score=109, Evalue=3e-25,

Paralogues:

None

Copy number: 496 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011611
- InterPro:   IPR002173
- InterPro:   IPR005926
- InterPro:   IPR017583 [H]

Pfam domain/function: PF00294 PfkB [H]

EC number: =2.7.1.144 [H]

Molecular weight: Translated: 32625; Mature: 32625

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS00584 PFKB_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVTVTPNPSIDRTVALPAPLTRGAVQRVTSVTSEPGGKGVNVARALTLARLDAVALLPA
CEEEECCCCCCCCEEECCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHEEEEEC
ADGDPMVTALRAIGVPFRCVPIAEPVRTNLAITESDGTTTKLNERGAALDAAARDALTRG
CCCCHHHHHHHHHCCCEEEEECCCCHHCCEEEECCCCCEEEECCCCCHHHHHHHHHHHHH
IIDAAEQASWVVLSGSLPPGLPDDWYAEVVARLVPLRCRVAVDTSERPLAALAAAFDHAA
HHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHCC
PDVIKPNAEELAGLVGASAEELESAAAQGDPEPVAAAARQLVDRGVGAVLATLGAAGAVL
CCCCCCCHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
VDRTGAWMATPPPIVPRSTVGAGDASLAGYVRAAVAGAEPPRRLRMAVAYGSAAAALPGS
EECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHEEEEEEECCHHHCCCCC
ALPSPAQLDLDGVAVSTIAPTPASS
CCCCCCCCCCCCEEEEEECCCCCCC
>Mature Secondary Structure
MIVTVTPNPSIDRTVALPAPLTRGAVQRVTSVTSEPGGKGVNVARALTLARLDAVALLPA
CEEEECCCCCCCCEEECCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHEEEEEC
ADGDPMVTALRAIGVPFRCVPIAEPVRTNLAITESDGTTTKLNERGAALDAAARDALTRG
CCCCHHHHHHHHHCCCEEEEECCCCHHCCEEEECCCCCEEEECCCCCHHHHHHHHHHHHH
IIDAAEQASWVVLSGSLPPGLPDDWYAEVVARLVPLRCRVAVDTSERPLAALAAAFDHAA
HHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHCC
PDVIKPNAEELAGLVGASAEELESAAAQGDPEPVAAAARQLVDRGVGAVLATLGAAGAVL
CCCCCCCHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
VDRTGAWMATPPPIVPRSTVGAGDASLAGYVRAAVAGAEPPRRLRMAVAYGSAAAALPGS
EECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHEEEEEEECCHHHCCCCC
ALPSPAQLDLDGVAVSTIAPTPASS
CCCCCCCCCCCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12354221 [H]