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The map label for this gene is gspE [H]

Identifier: 108763191

GI number: 108763191

Start: 2921231

End: 2923048

Strand: Reverse

Name: gspE [H]

Synonym: MXAN_2513

Alternate gene names: 108763191

Gene position: 2923048-2921231 (Counterclockwise)

Preceding gene: 108757430

Following gene: 108761173

Centisome position: 31.98

GC content: 67.16

Gene sequence:

>1818_bases
ATGAACCTGACCGCCGACCCCGCCCTCACCAGCGCCGCCACCGGAGGCACGGTTTCCCCGCGCAACGACGCCACGCAGCT
CGTGGCACACGGACAGGCCTACCTGTGTGGCCGCCCGCTGGGGGAAATCCTGCGCGCCATCGTCCCCTCGCTCACCGAGG
AGAAGCTCCAGGAGGCGCTCGCCATCCAGGACGAGAAGGGGCAGCGCATTGGCGAGGCCCTCGTGGGGATGAAGGCGGTC
TCCGAGGAGGACGTGGCCAAGGCCCTGGGGCACCAGTTGGACCTGCCCTACCTGGCGCGCATCTTCGCCGAAGAGGTGGA
CGCGGAGCTCGTCAAGCGCATCCCCATCAACTTCGCCAAGCAGTCACGCATCCTCCCGCTGTCCCTGGAGGGTGACACGG
TGGCGGTGGCGGTGGCGGACCCGCTGGACACCGCCGCGCTGGACCACGTGCGTGTGCTGCTGGGCCAGAGCGTCAGCCAG
CGCATCGCGCTGGGCTCCACCATCACCGACGCCATCAACAGCGTCTACGACCGCTCCGTCAACGAGACGGAACAGCTCGT
GGACGAGATGGAAACGCAGGACCTGGACGCCATCGCCCACGAGCTGGACGAGCCCAAGGACCTGCTCGACGAGGACGACG
AGGCGCCCGTCATCCGGCTGGTGAACTCCGTGCTGTTCCGCGCCGCCAAGGAGCGCGCCAGCGATATCCACATCGAGCCG
ATGGAGCGCGAGCTGCTGGTGCGCTTCCGCGTGGACGGTGTGCTGCAGGAGGTCATCAAGCCGCCCAAGCGCTACCAGAA
CGCCATCGTCAGCCGCGTGAAGGTCATGGGGCAGCTCAACATCGCGGAGAAGCGCCTGCCGCAGGACGGCCGCATCCGCA
TCAAGCTGGCCGGCCGCGACATCGACATCCGTCTGTCCACCATCCCCACGTCCTTCGGCGAGCGCATCGTCATGCGTCTG
CTGGACAAGACGGCGACGCTGCTGGACCTGGCGGAAATCGGCATGAGCCAGAAGACGCTCGAGTCGATGGAAGCCGTCAT
CAAACGCTCGCACGGCATCATCCTGGTGACGGGCCCCACGGGCTCCGGCAAGACGACGACGCTCTACGGCGCCCTGTCGA
AAATCAATACGCCCGACCTCAACATCCTCACCGTCGAGGACCCGGTCGAATACCAGCTCAAGGGCATTGGCCAGATGGCC
ATCAGCCCGAAGATTGGGCTGACGTTCGCGCAGGGGCTGCGCTCCTTCCTCCGCCAGGACCCGGACGTCATCATGGTCGG
CGAGATTCGCGACAAGGAGACGGCGGAAATCGCCATCCAGGCCTCGCTGACGGGCCACCTGGTGCTGTCCACCGTCCACA
CCAACGACGCCGCCGGCGCCGTGACACGTCTGGTGGACATGGGCGTGGAGCCCTTCCTCGTGGCGTCCTCGCTCACCGGC
ATCCTGGCCCAGCGGCTGGTGCGCCGCGTGTGCCCGGACTGCCGCGTGCCCTTCGAGCCCACCGACGCGGAGCTCAAGGA
GCTGGGCCACTCGGTGGCCTCCTTCAAGCAGCGCTACGGCGTGGACCGCATCTACAAGGCCTCCGGTTGCCCCTCCTGCA
ACCGCAACGGCTACCGCGGGCGAACCGGCATCTACGAGTTCCTCCCCGTGGATGACGACGTGCGCCAGCTCGTGCTGAAG
AACGTGGACGCCTCCACCATCAAGCGGTCCGCCACGTCCAAGGGCATGACGACGCTGCTGGATGACGGCGCGCGGAAGAT
TGCCCTGGGCGAGACGACCATCGCCGAGGTGCTCAGCATCACCCAGGAGGACATGTAG

Upstream 100 bases:

>100_bases
TCGAGCAGCCCCCGCCCGAGGCCATCCAGGTCCCCGAGGCAGGAGACGCCGAACGCCTGCGCATCCAGCACATCGAGCCG
GGGCCGAGGGAGTAGACGCC

Downstream 100 bases:

>100_bases
CCGACCGCTCCTCCCTGGGAGGGGCCGGGGTTCCTGAACCATGCCGGTCTTCGAGTACAGAGGTCTCAATTCCGCGGGCA
AGCAGATCAAGGGCCTGCTC

Product: general secretory pathway protein E

Products: NA

Alternate protein names: Cholera toxin secretion protein epsE; Type II traffic warden ATPase [H]

Number of amino acids: Translated: 605; Mature: 605

Protein sequence:

>605_residues
MNLTADPALTSAATGGTVSPRNDATQLVAHGQAYLCGRPLGEILRAIVPSLTEEKLQEALAIQDEKGQRIGEALVGMKAV
SEEDVAKALGHQLDLPYLARIFAEEVDAELVKRIPINFAKQSRILPLSLEGDTVAVAVADPLDTAALDHVRVLLGQSVSQ
RIALGSTITDAINSVYDRSVNETEQLVDEMETQDLDAIAHELDEPKDLLDEDDEAPVIRLVNSVLFRAAKERASDIHIEP
MERELLVRFRVDGVLQEVIKPPKRYQNAIVSRVKVMGQLNIAEKRLPQDGRIRIKLAGRDIDIRLSTIPTSFGERIVMRL
LDKTATLLDLAEIGMSQKTLESMEAVIKRSHGIILVTGPTGSGKTTTLYGALSKINTPDLNILTVEDPVEYQLKGIGQMA
ISPKIGLTFAQGLRSFLRQDPDVIMVGEIRDKETAEIAIQASLTGHLVLSTVHTNDAAGAVTRLVDMGVEPFLVASSLTG
ILAQRLVRRVCPDCRVPFEPTDAELKELGHSVASFKQRYGVDRIYKASGCPSCNRNGYRGRTGIYEFLPVDDDVRQLVLK
NVDASTIKRSATSKGMTTLLDDGARKIALGETTIAEVLSITQEDM

Sequences:

>Translated_605_residues
MNLTADPALTSAATGGTVSPRNDATQLVAHGQAYLCGRPLGEILRAIVPSLTEEKLQEALAIQDEKGQRIGEALVGMKAV
SEEDVAKALGHQLDLPYLARIFAEEVDAELVKRIPINFAKQSRILPLSLEGDTVAVAVADPLDTAALDHVRVLLGQSVSQ
RIALGSTITDAINSVYDRSVNETEQLVDEMETQDLDAIAHELDEPKDLLDEDDEAPVIRLVNSVLFRAAKERASDIHIEP
MERELLVRFRVDGVLQEVIKPPKRYQNAIVSRVKVMGQLNIAEKRLPQDGRIRIKLAGRDIDIRLSTIPTSFGERIVMRL
LDKTATLLDLAEIGMSQKTLESMEAVIKRSHGIILVTGPTGSGKTTTLYGALSKINTPDLNILTVEDPVEYQLKGIGQMA
ISPKIGLTFAQGLRSFLRQDPDVIMVGEIRDKETAEIAIQASLTGHLVLSTVHTNDAAGAVTRLVDMGVEPFLVASSLTG
ILAQRLVRRVCPDCRVPFEPTDAELKELGHSVASFKQRYGVDRIYKASGCPSCNRNGYRGRTGIYEFLPVDDDVRQLVLK
NVDASTIKRSATSKGMTTLLDDGARKIALGETTIAEVLSITQEDM
>Mature_605_residues
MNLTADPALTSAATGGTVSPRNDATQLVAHGQAYLCGRPLGEILRAIVPSLTEEKLQEALAIQDEKGQRIGEALVGMKAV
SEEDVAKALGHQLDLPYLARIFAEEVDAELVKRIPINFAKQSRILPLSLEGDTVAVAVADPLDTAALDHVRVLLGQSVSQ
RIALGSTITDAINSVYDRSVNETEQLVDEMETQDLDAIAHELDEPKDLLDEDDEAPVIRLVNSVLFRAAKERASDIHIEP
MERELLVRFRVDGVLQEVIKPPKRYQNAIVSRVKVMGQLNIAEKRLPQDGRIRIKLAGRDIDIRLSTIPTSFGERIVMRL
LDKTATLLDLAEIGMSQKTLESMEAVIKRSHGIILVTGPTGSGKTTTLYGALSKINTPDLNILTVEDPVEYQLKGIGQMA
ISPKIGLTFAQGLRSFLRQDPDVIMVGEIRDKETAEIAIQASLTGHLVLSTVHTNDAAGAVTRLVDMGVEPFLVASSLTG
ILAQRLVRRVCPDCRVPFEPTDAELKELGHSVASFKQRYGVDRIYKASGCPSCNRNGYRGRTGIYEFLPVDDDVRQLVLK
NVDASTIKRSATSKGMTTLLDDGARKIALGETTIAEVLSITQEDM

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=490, Percent_Identity=46.530612244898, Blast_Score=443, Evalue=1e-125,
Organism=Escherichia coli, GI1786296, Length=395, Percent_Identity=43.2911392405063, Blast_Score=332, Evalue=4e-92,
Organism=Escherichia coli, GI87082188, Length=151, Percent_Identity=49.0066225165563, Blast_Score=122, Evalue=5e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 65971; Mature: 65971

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLTADPALTSAATGGTVSPRNDATQLVAHGQAYLCGRPLGEILRAIVPSLTEEKLQEAL
CCCCCCCCHHCCCCCCCCCCCCHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHHHHHHH
AIQDEKGQRIGEALVGMKAVSEEDVAKALGHQLDLPYLARIFAEEVDAELVKRIPINFAK
HCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHC
QSRILPLSLEGDTVAVAVADPLDTAALDHVRVLLGQSVSQRIALGSTITDAINSVYDRSV
CCCEEEEEECCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCC
NETEQLVDEMETQDLDAIAHELDEPKDLLDEDDEAPVIRLVNSVLFRAAKERASDIHIEP
CHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
MERELLVRFRVDGVLQEVIKPPKRYQNAIVSRVKVMGQLNIAEKRLPQDGRIRIKLAGRD
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCEEEEEECCC
IDIRLSTIPTSFGERIVMRLLDKTATLLDLAEIGMSQKTLESMEAVIKRSHGIILVTGPT
EEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEECCC
GSGKTTTLYGALSKINTPDLNILTVEDPVEYQLKGIGQMAISPKIGLTFAQGLRSFLRQD
CCCCCEEHHHHHHHCCCCCCEEEEECCCHHHHHHCCCCEECCCCCCHHHHHHHHHHHHCC
PDVIMVGEIRDKETAEIAIQASLTGHLVLSTVHTNDAAGAVTRLVDMGVEPFLVASSLTG
CCEEEEECCCCCCCEEEEEEEECCCEEEEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHH
ILAQRLVRRVCPDCRVPFEPTDAELKELGHSVASFKQRYGVDRIYKASGCPSCNRNGYRG
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHCCCCCCCCCCCCCC
RTGIYEFLPVDDDVRQLVLKNVDASTIKRSATSKGMTTLLDDGARKIALGETTIAEVLSI
CCCCEEECCCCHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHCCCCEEEECHHHHHHHHHH
TQEDM
HHCCC
>Mature Secondary Structure
MNLTADPALTSAATGGTVSPRNDATQLVAHGQAYLCGRPLGEILRAIVPSLTEEKLQEAL
CCCCCCCCHHCCCCCCCCCCCCHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHHHHHHH
AIQDEKGQRIGEALVGMKAVSEEDVAKALGHQLDLPYLARIFAEEVDAELVKRIPINFAK
HCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHC
QSRILPLSLEGDTVAVAVADPLDTAALDHVRVLLGQSVSQRIALGSTITDAINSVYDRSV
CCCEEEEEECCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCC
NETEQLVDEMETQDLDAIAHELDEPKDLLDEDDEAPVIRLVNSVLFRAAKERASDIHIEP
CHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
MERELLVRFRVDGVLQEVIKPPKRYQNAIVSRVKVMGQLNIAEKRLPQDGRIRIKLAGRD
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCEEEEEECCC
IDIRLSTIPTSFGERIVMRLLDKTATLLDLAEIGMSQKTLESMEAVIKRSHGIILVTGPT
EEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEECCC
GSGKTTTLYGALSKINTPDLNILTVEDPVEYQLKGIGQMAISPKIGLTFAQGLRSFLRQD
CCCCCEEHHHHHHHCCCCCCEEEEECCCHHHHHHCCCCEECCCCCCHHHHHHHHHHHHCC
PDVIMVGEIRDKETAEIAIQASLTGHLVLSTVHTNDAAGAVTRLVDMGVEPFLVASSLTG
CCEEEEECCCCCCCEEEEEEEECCCEEEEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHH
ILAQRLVRRVCPDCRVPFEPTDAELKELGHSVASFKQRYGVDRIYKASGCPSCNRNGYRG
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHCCCCCCCCCCCCCC
RTGIYEFLPVDDDVRQLVLKNVDASTIKRSATSKGMTTLLDDGARKIALGETTIAEVLSI
CCCCEEECCCCHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHCCCCEEEECHHHHHHHHHH
TQEDM
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8423007; 10952301 [H]