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The map label for this gene is gspF [H]

Identifier: 108761173

GI number: 108761173

Start: 2919934

End: 2921190

Strand: Reverse

Name: gspF [H]

Synonym: MXAN_2512

Alternate gene names: 108761173

Gene position: 2921190-2919934 (Counterclockwise)

Preceding gene: 108763191

Following gene: 108760464

Centisome position: 31.96

GC content: 66.11

Gene sequence:

>1257_bases
ATGCCGGTCTTCGAGTACAGAGGTCTCAATTCCGCGGGCAAGCAGATCAAGGGCCTGCTCGAGGCGGACTCACCCAAGAC
GCTGCGGTCCAAGCTGCGCGCCGACGGCATCTTCCTCACGGATGTGCTGGCCCAGGCCGAAGGCAGCCGCGCCGCCGTGG
CCAAGGGCACCAACGCGGCGCTGGTGGCGCGCGACATCGACCTGCGCAAGCTGGGCCGGGGCCGCGTCAACACCGACGAC
GTGGCCATCTTCACCCGGCAGCTCTCCACGCTGCTGGGTGCGGGCGTCACGCTGGTGGAGTCGCTCAGCGCGCTGGTGGA
CCAGGTGGAGAAGGAGCGCTTCAAGCGCGCCCTCTCCGACATCAAGCAGCGCGTCAACGAAGGCTCGTCCCTGGCGGAAG
CCATGGGGCAGCACCCGAAAATCTTCCCCAGCATCTACGTGAACATGGTGCGCGCGGGTGAGGCCTCCGGCGCGCTGGAC
GCGGTGCTCACGCGCCTGGCGGACTTCACGGAGAACCAGGCCCGGCTGCAGCAGAAGATTCTCAGCACCATGCTCTACCC
CGCCATCATGATGGTGGTGGGCGGCGGGATCCTCGTGGCCCTCATGGTCTTCGTGGTGCCGAAGGTGACGAAAATCTTCG
AGACGATGAAGGCCACGCTGCCCCTGAGCACGCGCTTCCTCATCGCCTCCAGCAACTTCTTCCAGAGCTGGTGGTTCATC
CTGCTGCCGGCCATGGCGCTGGGCGTGGTCCTCTTCATGCGCTGGACGAAGAGCCCCTCGGGAAAGCCCAAGTGGGACCG
CATCACGCTCAAGGCCCCCGTGGTGGGCAACCTGGTGCGCCTGCTGTCCATCTCCCGCTTCGCCCGCACGCTGTCCACGC
TGCTCAAGAGCGGCGTCCCGCTGCTGGCGGCCATGGACATCGTCAAGGCCATCATGACCAACACCGTCCTGGCGGAGGTC
GTCGAGAAGGCCCGCGACTCCATCCGCGAGGGCGAGAGCATCGCCAACCCGCTGAAGCGCTCCGGGGAATTCCCTCCGCT
GGTGTACCACATGGTCGCCATCGGTGAGCGCTCCGGCCAGTTGGAGGAGATGCTCACCAGCGTGGCGGACAACTACGAGA
CGCAGGTGAACGTGCGCATCAGCGCCCTCACCTCGCTGCTGGAGCCCCTCCTCATCGTGGTGATGGGCGCGGTGATTGCA
TTCGTCGCGCTCTCCATCCTGATGCCGATTCTGCAGGTGAACTCGGCCATCCGGTGA

Upstream 100 bases:

>100_bases
ATTGCCCTGGGCGAGACGACCATCGCCGAGGTGCTCAGCATCACCCAGGAGGACATGTAGCCGACCGCTCCTCCCTGGGA
GGGGCCGGGGTTCCTGAACC

Downstream 100 bases:

>100_bases
GGTGTGGCGGAATGATGAACGACATGGCGGACCGTTGGATTCAACGCGTCACCCTGGCGGCAATGGTCGCCATGGCGGCG
GCGCTGCTGACCGTGGGGGC

Product: general secretion pathway protein F

Products: NA

Alternate protein names: Cholera toxin secretion protein epsF [H]

Number of amino acids: Translated: 418; Mature: 417

Protein sequence:

>418_residues
MPVFEYRGLNSAGKQIKGLLEADSPKTLRSKLRADGIFLTDVLAQAEGSRAAVAKGTNAALVARDIDLRKLGRGRVNTDD
VAIFTRQLSTLLGAGVTLVESLSALVDQVEKERFKRALSDIKQRVNEGSSLAEAMGQHPKIFPSIYVNMVRAGEASGALD
AVLTRLADFTENQARLQQKILSTMLYPAIMMVVGGGILVALMVFVVPKVTKIFETMKATLPLSTRFLIASSNFFQSWWFI
LLPAMALGVVLFMRWTKSPSGKPKWDRITLKAPVVGNLVRLLSISRFARTLSTLLKSGVPLLAAMDIVKAIMTNTVLAEV
VEKARDSIREGESIANPLKRSGEFPPLVYHMVAIGERSGQLEEMLTSVADNYETQVNVRISALTSLLEPLLIVVMGAVIA
FVALSILMPILQVNSAIR

Sequences:

>Translated_418_residues
MPVFEYRGLNSAGKQIKGLLEADSPKTLRSKLRADGIFLTDVLAQAEGSRAAVAKGTNAALVARDIDLRKLGRGRVNTDD
VAIFTRQLSTLLGAGVTLVESLSALVDQVEKERFKRALSDIKQRVNEGSSLAEAMGQHPKIFPSIYVNMVRAGEASGALD
AVLTRLADFTENQARLQQKILSTMLYPAIMMVVGGGILVALMVFVVPKVTKIFETMKATLPLSTRFLIASSNFFQSWWFI
LLPAMALGVVLFMRWTKSPSGKPKWDRITLKAPVVGNLVRLLSISRFARTLSTLLKSGVPLLAAMDIVKAIMTNTVLAEV
VEKARDSIREGESIANPLKRSGEFPPLVYHMVAIGERSGQLEEMLTSVADNYETQVNVRISALTSLLEPLLIVVMGAVIA
FVALSILMPILQVNSAIR
>Mature_417_residues
PVFEYRGLNSAGKQIKGLLEADSPKTLRSKLRADGIFLTDVLAQAEGSRAAVAKGTNAALVARDIDLRKLGRGRVNTDDV
AIFTRQLSTLLGAGVTLVESLSALVDQVEKERFKRALSDIKQRVNEGSSLAEAMGQHPKIFPSIYVNMVRAGEASGALDA
VLTRLADFTENQARLQQKILSTMLYPAIMMVVGGGILVALMVFVVPKVTKIFETMKATLPLSTRFLIASSNFFQSWWFIL
LPAMALGVVLFMRWTKSPSGKPKWDRITLKAPVVGNLVRLLSISRFARTLSTLLKSGVPLLAAMDIVKAIMTNTVLAEVV
EKARDSIREGESIANPLKRSGEFPPLVYHMVAIGERSGQLEEMLTSVADNYETQVNVRISALTSLLEPLLIVVMGAVIAF
VALSILMPILQVNSAIR

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG1459

COG function: function code NU; Type II secretory pathway, component PulF

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP F family [H]

Homologues:

Organism=Escherichia coli, GI1789724, Length=414, Percent_Identity=33.8164251207729, Blast_Score=263, Evalue=2e-71,
Organism=Escherichia coli, GI1786295, Length=346, Percent_Identity=28.0346820809249, Blast_Score=149, Evalue=2e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003004
- InterPro:   IPR011850
- InterPro:   IPR018076
- InterPro:   IPR001992 [H]

Pfam domain/function: PF00482 GSPII_F [H]

EC number: NA

Molecular weight: Translated: 45535; Mature: 45404

Theoretical pI: Translated: 10.56; Mature: 10.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVFEYRGLNSAGKQIKGLLEADSPKTLRSKLRADGIFLTDVLAQAEGSRAAVAKGTNAA
CCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHCCCCCCHHCCCCCCE
LVARDIDLRKLGRGRVNTDDVAIFTRQLSTLLGAGVTLVESLSALVDQVEKERFKRALSD
EEEECCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IKQRVNEGSSLAEAMGQHPKIFPSIYVNMVRAGEASGALDAVLTRLADFTENQARLQQKI
HHHHHCCCHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHH
LSTMLYPAIMMVVGGGILVALMVFVVPKVTKIFETMKATLPLSTRFLIASSNFFQSWWFI
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHH
LLPAMALGVVLFMRWTKSPSGKPKWDRITLKAPVVGNLVRLLSISRFARTLSTLLKSGVP
HHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCH
LLAAMDIVKAIMTNTVLAEVVEKARDSIREGESIANPLKRSGEFPPLVYHMVAIGERSGQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCH
LEEMLTSVADNYETQVNVRISALTSLLEPLLIVVMGAVIAFVALSILMPILQVNSAIR
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PVFEYRGLNSAGKQIKGLLEADSPKTLRSKLRADGIFLTDVLAQAEGSRAAVAKGTNAA
CCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHCCCCCCHHCCCCCCE
LVARDIDLRKLGRGRVNTDDVAIFTRQLSTLLGAGVTLVESLSALVDQVEKERFKRALSD
EEEECCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IKQRVNEGSSLAEAMGQHPKIFPSIYVNMVRAGEASGALDAVLTRLADFTENQARLQQKI
HHHHHCCCHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHH
LSTMLYPAIMMVVGGGILVALMVFVVPKVTKIFETMKATLPLSTRFLIASSNFFQSWWFI
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHH
LLPAMALGVVLFMRWTKSPSGKPKWDRITLKAPVVGNLVRLLSISRFARTLSTLLKSGVP
HHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCH
LLAAMDIVKAIMTNTVLAEVVEKARDSIREGESIANPLKRSGEFPPLVYHMVAIGERSGQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHCCCCCH
LEEMLTSVADNYETQVNVRISALTSLLEPLLIVVMGAVIAFVALSILMPILQVNSAIR
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]