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The map label for this gene is dusA [H]

Identifier: 108761431

GI number: 108761431

Start: 2650879

End: 2651877

Strand: Direct

Name: dusA [H]

Synonym: MXAN_2283

Alternate gene names: 108761431

Gene position: 2650879-2651877 (Clockwise)

Preceding gene: 108762231

Following gene: 108758552

Centisome position: 29.0

GC content: 69.97

Gene sequence:

>999_bases
ATGATGCCGACTCATCCCATGCCGCTGTCTGTCGCGCCGATGATGGACTGGACGGACCGCAACTGCCGGTACTTCCATCG
CCAGCTCAGCCGCCACACGCTGCTTTACACGGAGATGGTCACCACGGGCGCAATCCTGCATGGCGACCGGGAGCGGCTGC
TGGGCTACGACGCCGCGGAGCACCCGGTGGCGCTTCAGCTTGGCGGTTCGGAGCCGGCGGCGCTCGCCGAGTCCGCCCGC
ATCGGCGAGCAGTGGGGCTACGACGAAATCAACCTCAACGTGGGTTGCCCGAGCGACCGCGTCCAGTCGGGCCGCTTCGG
CGCGTGCCTGATGGCGGAGCCGGACCTGGTGGCTCGGCTGGTGGGGGCGATGCGAGAGGCGGTGAGCATCCCGGTGACGG
TGAAGTCGCGCATCGCCATCGACGAGTTGGAGGAGTGGCCCACGCTGGAGGGCTTCATCCGGAAGGTGTCCGCCGCGGGC
TGCACCCGCTTCATCGTGCACGCGCGCAAGGCATGGCTCCAGGGGCTGAGCCCCAAGGAGAACCGCGACGTCCCGCCGCT
GCGCTACGACCTGGTGCACCGGCTCAAGGTGGAGTTCCCGCACCTGGACATCAGCATCAACGGCGGCATCAAGACGCTGG
ACGCCGCCGCGGAGCAGTTGCGCCATGTGGACGGGGTGATGATGGGCCGCGCCCCGTATGAGAACCCGTACCTGCTGGCC
GAGGCCGACCGCCGTTTCTTCGGCGCCACCACGCCGGTGCCGCAGCGGCACGAAGTCGTCGAGGCCATGCTGCCGTACAT
CGAGAAGGCCATGCTGCGCGGCGCGCCGCTGGGCGCCATCACCCGACACATGCTGGGGCTCTTCCAGGGGCTCCCGGGGG
CGCGCGCGTGGCGCAGGCACCTGAGTGAGAATGCCCACAAGCAGGGCGCCGGGCCGGAGGTCGTCGTGGCGGCGCTGGCG
AAGGTGCGGCGGGAGCCCGAAGCCGAAGTCGCGGCCTGA

Upstream 100 bases:

>100_bases
CGGCAAAGGGGTGGGCAGGGGCGGAAAACCCGGCTACATGCAGGCGGCAGGGGAGCGGCCCGCCGAACGGGACGCTCCGG
TTGCTGCCCCGGGTTCTCGC

Downstream 100 bases:

>100_bases
CTGGGGCATCACCCCGACACCAGAGGGAACCGCAGGTACGAGCCGCTTCCTTTCGGCCCGGCATGCGATTCCCTCTGGCC
CACCGCTGCGCTCGGCGTGG

Product: tRNA-dihydrouridine synthase A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MMPTHPMPLSVAPMMDWTDRNCRYFHRQLSRHTLLYTEMVTTGAILHGDRERLLGYDAAEHPVALQLGGSEPAALAESAR
IGEQWGYDEINLNVGCPSDRVQSGRFGACLMAEPDLVARLVGAMREAVSIPVTVKSRIAIDELEEWPTLEGFIRKVSAAG
CTRFIVHARKAWLQGLSPKENRDVPPLRYDLVHRLKVEFPHLDISINGGIKTLDAAAEQLRHVDGVMMGRAPYENPYLLA
EADRRFFGATTPVPQRHEVVEAMLPYIEKAMLRGAPLGAITRHMLGLFQGLPGARAWRRHLSENAHKQGAGPEVVVAALA
KVRREPEAEVAA

Sequences:

>Translated_332_residues
MMPTHPMPLSVAPMMDWTDRNCRYFHRQLSRHTLLYTEMVTTGAILHGDRERLLGYDAAEHPVALQLGGSEPAALAESAR
IGEQWGYDEINLNVGCPSDRVQSGRFGACLMAEPDLVARLVGAMREAVSIPVTVKSRIAIDELEEWPTLEGFIRKVSAAG
CTRFIVHARKAWLQGLSPKENRDVPPLRYDLVHRLKVEFPHLDISINGGIKTLDAAAEQLRHVDGVMMGRAPYENPYLLA
EADRRFFGATTPVPQRHEVVEAMLPYIEKAMLRGAPLGAITRHMLGLFQGLPGARAWRRHLSENAHKQGAGPEVVVAALA
KVRREPEAEVAA
>Mature_332_residues
MMPTHPMPLSVAPMMDWTDRNCRYFHRQLSRHTLLYTEMVTTGAILHGDRERLLGYDAAEHPVALQLGGSEPAALAESAR
IGEQWGYDEINLNVGCPSDRVQSGRFGACLMAEPDLVARLVGAMREAVSIPVTVKSRIAIDELEEWPTLEGFIRKVSAAG
CTRFIVHARKAWLQGLSPKENRDVPPLRYDLVHRLKVEFPHLDISINGGIKTLDAAAEQLRHVDGVMMGRAPYENPYLLA
EADRRFFGATTPVPQRHEVVEAMLPYIEKAMLRGAPLGAITRHMLGLFQGLPGARAWRRHLSENAHKQGAGPEVVVAALA
KVRREPEAEVAA

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusA subfamily [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=288, Percent_Identity=25.3472222222222, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI40807366, Length=293, Percent_Identity=26.962457337884, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI145693211, Length=315, Percent_Identity=63.1746031746032, Blast_Score=399, Evalue=1e-112,
Organism=Escherichia coli, GI1789660, Length=238, Percent_Identity=26.4705882352941, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1788462, Length=180, Percent_Identity=32.7777777777778, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI25144369, Length=225, Percent_Identity=31.1111111111111, Blast_Score=71, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI17507177, Length=167, Percent_Identity=27.5449101796407, Blast_Score=68, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6323560, Length=286, Percent_Identity=21.6783216783217, Blast_Score=73, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24580595, Length=293, Percent_Identity=26.2798634812287, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI19920448, Length=293, Percent_Identity=26.2798634812287, Blast_Score=76, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004653
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: 1.-.-.-

Molecular weight: Translated: 36809; Mature: 36809

Theoretical pI: Translated: 7.43; Mature: 7.43

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMPTHPMPLSVAPMMDWTDRNCRYFHRQLSRHTLLYTEMVTTGAILHGDRERLLGYDAAE
CCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCHHHHHCCCCCC
HPVALQLGGSEPAALAESARIGEQWGYDEINLNVGCPSDRVQSGRFGACLMAEPDLVARL
CCEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEECCCHHHHCCCCCCEEEECCHHHHHHH
VGAMREAVSIPVTVKSRIAIDELEEWPTLEGFIRKVSAAGCTRFIVHARKAWLQGLSPKE
HHHHHHHHCCCEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
NRDVPPLRYDLVHRLKVEFPHLDISINGGIKTLDAAAEQLRHVDGVMMGRAPYENPYLLA
CCCCCCHHHHHHHHHHEECCEEEEEECCCCHHHHHHHHHHHHHHHHEECCCCCCCCEEEE
EADRRFFGATTPVPQRHEVVEAMLPYIEKAMLRGAPLGAITRHMLGLFQGLPGARAWRRH
ECCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHH
LSENAHKQGAGPEVVVAALAKVRREPEAEVAA
HHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MMPTHPMPLSVAPMMDWTDRNCRYFHRQLSRHTLLYTEMVTTGAILHGDRERLLGYDAAE
CCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCHHHHHCCCCCC
HPVALQLGGSEPAALAESARIGEQWGYDEINLNVGCPSDRVQSGRFGACLMAEPDLVARL
CCEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEECCCHHHHCCCCCCEEEECCHHHHHHH
VGAMREAVSIPVTVKSRIAIDELEEWPTLEGFIRKVSAAGCTRFIVHARKAWLQGLSPKE
HHHHHHHHCCCEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
NRDVPPLRYDLVHRLKVEFPHLDISINGGIKTLDAAAEQLRHVDGVMMGRAPYENPYLLA
CCCCCCHHHHHHHHHHEECCEEEEEECCCCHHHHHHHHHHHHHHHHEECCCCCCCCEEEE
EADRRFFGATTPVPQRHEVVEAMLPYIEKAMLRGAPLGAITRHMLGLFQGLPGARAWRRH
ECCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHH
LSENAHKQGAGPEVVVAALAKVRREPEAEVAA
HHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]