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The map label for this gene is rph

Identifier: 108758625

GI number: 108758625

Start: 2336758

End: 2337471

Strand: Direct

Name: rph

Synonym: MXAN_2004

Alternate gene names: 108758625

Gene position: 2336758-2337471 (Clockwise)

Preceding gene: 108758052

Following gene: 108760288

Centisome position: 25.57

GC content: 70.03

Gene sequence:

>714_bases
GTGCGTTCCTTTCAACGTGGTGCGCTAGACCTTCGCCCCGTCGTCCTCACCCCCGGTGTCTCCCGCTACGCGGAGGGCTC
GGTGCAGGTGGAGTTCGGCCACACCAAGGTGCTCGTCACCTGCTCCACGGAGGAGCGCGTTCCGCCGCACCTGATGGGCA
AGGGCTCCGGCTGGGTGACGGCGGAGTACGGCATGCTGCCGCGCGCCACGCACTCGCGGAACCAGCGTGAGTCCGCCAAG
GGCAAGCAGACGGGCCGCACCATGGAAATCCAGCGGCTCATCGGCCGCTCCCTGCGCGCCGCGGTGGACCTGTCCACCCT
GGGGCCTCGCACCCTGACGCTGGACTGTGACGTGCTCCAGGCGGACGGTGGCACCCGCACCGCTTCCATCACCGGGGCCT
ACGTGGCGCTGGTGCTGGCGCTGCGCTCGCTGCAGAAGGCCGGAACCATCTCCAAGCTGCCCAAGCTCACGCCGTTGGCG
GCCGTGTCCGTGGGCATCGTCAAGGGCGAGGTCCGGGTGGACCTGGACTACGACGAGGACTCCACCGCGGATGTGGACCT
GAACCTGGTGGCCACCGCGGACGGCCGCATGGTGGAGCTGCAGGGCACGGCGGAGCACCAGCTCTTCGACCGCAAGGCCC
TGGACGCCATGGTGGATGGCGGGCTGGCCGCCATCCAGAAGCTGACGGCCGCGCAGGCGCAGGTGCTGGGATGA

Upstream 100 bases:

>100_bases
CGTGTCGCCCTGAGTGCAACGGCGCCAAGGCAATCGACACCCGCGCGGCGTGGCTCTGGTAGAGAAGGGGACGCTCGTCC
TCATTTCCCAGGAGCCTGCT

Downstream 100 bases:

>100_bases
CGGTGAAGCCCCGGCTGCTGTTCGCGACGACCAACCAGGGCAAGCTGCGCGAGCTGCGTGGCCTCGTCGGAGACGCGGTG
GAGGTGGTGTCCCTGGCGGA

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase

Number of amino acids: Translated: 237; Mature: 237

Protein sequence:

>237_residues
MRSFQRGALDLRPVVLTPGVSRYAEGSVQVEFGHTKVLVTCSTEERVPPHLMGKGSGWVTAEYGMLPRATHSRNQRESAK
GKQTGRTMEIQRLIGRSLRAAVDLSTLGPRTLTLDCDVLQADGGTRTASITGAYVALVLALRSLQKAGTISKLPKLTPLA
AVSVGIVKGEVRVDLDYDEDSTADVDLNLVATADGRMVELQGTAEHQLFDRKALDAMVDGGLAAIQKLTAAQAQVLG

Sequences:

>Translated_237_residues
MRSFQRGALDLRPVVLTPGVSRYAEGSVQVEFGHTKVLVTCSTEERVPPHLMGKGSGWVTAEYGMLPRATHSRNQRESAK
GKQTGRTMEIQRLIGRSLRAAVDLSTLGPRTLTLDCDVLQADGGTRTASITGAYVALVLALRSLQKAGTISKLPKLTPLA
AVSVGIVKGEVRVDLDYDEDSTADVDLNLVATADGRMVELQGTAEHQLFDRKALDAMVDGGLAAIQKLTAAQAQVLG
>Mature_237_residues
MRSFQRGALDLRPVVLTPGVSRYAEGSVQVEFGHTKVLVTCSTEERVPPHLMGKGSGWVTAEYGMLPRATHSRNQRESAK
GKQTGRTMEIQRLIGRSLRAAVDLSTLGPRTLTLDCDVLQADGGTRTASITGAYVALVLALRSLQKAGTISKLPKLTPLA
AVSVGIVKGEVRVDLDYDEDSTADVDLNLVATADGRMVELQGTAEHQLFDRKALDAMVDGGLAAIQKLTAAQAQVLG

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family

Homologues:

Organism=Escherichia coli, GI157672248, Length=209, Percent_Identity=64.1148325358852, Blast_Score=259, Evalue=8e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNPH_MYXXD (Q1DAT4)

Other databases:

- EMBL:   CP000113
- RefSeq:   YP_630239.1
- ProteinModelPortal:   Q1DAT4
- SMR:   Q1DAT4
- STRING:   Q1DAT4
- GeneID:   4104498
- GenomeReviews:   CP000113_GR
- KEGG:   mxa:MXAN_2004
- TIGR:   MXAN_2004
- eggNOG:   COG0689
- HOGENOM:   HBG737187
- OMA:   MLPRATG
- PhylomeDB:   Q1DAT4
- ProtClustDB:   PRK00173
- BioCyc:   MXAN246197:MXAN_2004-MONOMER
- HAMAP:   MF_00564
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336
- TIGRFAMs:   TIGR01966

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C; SSF55666 3_ExoRNase; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.56

Molecular weight: Translated: 25265; Mature: 25265

Theoretical pI: Translated: 8.70; Mature: 8.70

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSFQRGALDLRPVVLTPGVSRYAEGSVQVEFGHTKVLVTCSTEERVPPHLMGKGSGWVT
CCCCCCCCCCCEEEEECCCCHHHCCCCEEEEECCEEEEEEECCCCCCCCCEECCCCCEEE
AEYGMLPRATHSRNQRESAKGKQTGRTMEIQRLIGRSLRAAVDLSTLGPRTLTLDCDVLQ
EECCCCCCCCCCCCHHHHHCHHHCCCHHHHHHHHHHHHHHHEECCCCCCEEEEEEEEEEE
ADGGTRTASITGAYVALVLALRSLQKAGTISKLPKLTPLAAVSVGIVKGEVRVDLDYDED
CCCCCEEEEHHHHHHHHHHHHHHHHHCCCHHHCCCCCHHHHEEEEEEEEEEEEEECCCCC
STADVDLNLVATADGRMVELQGTAEHQLFDRKALDAMVDGGLAAIQKLTAAQAQVLG
CCCCEEEEEEEECCCEEEEEECCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRSFQRGALDLRPVVLTPGVSRYAEGSVQVEFGHTKVLVTCSTEERVPPHLMGKGSGWVT
CCCCCCCCCCCEEEEECCCCHHHCCCCEEEEECCEEEEEEECCCCCCCCCEECCCCCEEE
AEYGMLPRATHSRNQRESAKGKQTGRTMEIQRLIGRSLRAAVDLSTLGPRTLTLDCDVLQ
EECCCCCCCCCCCCHHHHHCHHHCCCHHHHHHHHHHHHHHHEECCCCCCEEEEEEEEEEE
ADGGTRTASITGAYVALVLALRSLQKAGTISKLPKLTPLAAVSVGIVKGEVRVDLDYDED
CCCCCEEEEHHHHHHHHHHHHHHHHHCCCHHHCCCCCHHHHEEEEEEEEEEEEEECCCCC
STADVDLNLVATADGRMVELQGTAEHQLFDRKALDAMVDGGLAAIQKLTAAQAQVLG
CCCCEEEEEEEECCCEEEEEECCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA