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The map label for this gene is pbpB [H]

Identifier: 108563930

GI number: 108563930

Start: 1569074

End: 1570921

Strand: Reverse

Name: pbpB [H]

Synonym: HPAG1_1505

Alternate gene names: 108563930

Gene position: 1570921-1569074 (Counterclockwise)

Preceding gene: 108563931

Following gene: 108563927

Centisome position: 98.41

GC content: 40.1

Gene sequence:

>1848_bases
ATGGATAATAAAAACATTGATCCTAGCTTCAACCCAGAGCGATTTTTAGAAACCCAAAAATACAAGGGTTCTGTTACAGC
ATTAATCTTTTTATTGCTTTTTATCGTTTTTTTAATGGTGGCTTTTAAAAAAGCTTTTTTTGCCCAAGCCAACATGCCCA
CTCTAGTGATGAGCAAACAAGACACTGCGACTAGGGGGACTATCTATAGTCAAGACAACTACAGCCTGGCCGCTTCGCAA
ACCCTTTTCAAACTGGGCTTTGACACGAGGTTTTTAAACCCGGATAAAGAAGATTTTTTCGTTGATTTCCTTTCTATTTA
TAGCAATATCCCTAAAAAGTCCTTAAAAGACGCCATCAATACGAAAGGCTATATCATTCTAGCCTACGATCTCACGCCCA
ATATGGCTGCTAATATTAGAGACTTGAATAAGAAATTTTTAACCTTTGGGGTTTTTCAAAATTTCAAAGACGCGCACGAT
AAAGTGTGGCAAAAGCAAGGGCTAAACATTGAAGTGAGCGGCGTTTCTAGGCATTACCCTTATCAAAATAGCCTAGAGCC
AATCATTGGCTATGTGCAAAAACAAGAAGAAGACAAGCTCACTTTAACTACCGGTAAAAAAGGCGTTGAAAAATCTCAAG
ATCACTTGCTTAAAGCCCAACAAAATGGCATAAGAACGGGCAAAAGAGATGTGAGTTTTAACTTTATCCAAAACCACTCT
TATACAGAGGTTGAGCGGCTTGATGGCTATGAGGTGTATTTGAGCGTTCCTTTAAAGCTCCAAAGAGAAATTGAAACCCT
ATTAGATAAAACTAAAGACAAACTCAAGGCTGAAGAAATCCTAGTGGGTATCATTAACCCTAAAAGCGGGGAAATTTTAT
CGCTGGCTTCAAGCAAGCGCTTCAATCCTAATGCGATTAAAACCAGCGATTATGAAAGCTTGAATTTGAGCGTTGCCGAA
AAGGTTTTTGAGCCAGGTAGCACGATCAAACCCATTGTTTATTCCTTGCTGTTAGACAAGAATTTGATCAACCCTAAAGA
ACGCATTGATTTAAACCATGGCTATTACCAATTAGGAAAATACACCATTAAAGACGACTTTATCCCTAGTAAAAAAGCCG
TTATGGAAGACATTTTGATCCAATCTAGCAATGTGGGCATGATAAAAATCAGCAAAAACCTCAACCCAGAAGATTTCTAT
AACGGGCTTTTAGGCTATGGATTTTCTCAAAAAACGGGCATTGATTTATCCTTAGAAGCCACAGGAAAGATCCCTCCTTT
GTCCGCTTTCAAGCGTGAAGTGTTAAAGGGGAGCGTCTCTTATGGCTATGGGCTGAACGCGACTTTTTTGCAGCTTTTAA
GGGCTTATGCGGTGTTTTCTAATGAAGGCAAATTGACTACCCCCTATTTAGTGCAACGAGAGACCGCCCCTAATGGCGAT
ATTTACATCCCCAGCCCCAAACCCACTTTTCAAGTCATTAGCCCAAAAAGTGCTAGAAAAATGAAAGAAACCTTAATCAA
AGTGGTGCGTTATGGCACAGGCAAAAACGCTCAATTTGAAGGGCTATACATAGGGGGCAAAACAGGCACGGCTAGGGTTG
CTAAAAACGGGAGTTATAGCGTGCAGTCCTACAACAGCTCTTTTTTTGGGTTCGCTGAAGATGAAAGGCAGGTTTTTACT
ATCGGCGTGGTTATCTTAGGTTCGCACGGCAAGGAAGAGTATTACGCTAGCAAGATTGCAGCCCCCATTTTTAAAGAAAT
CACTGAAATTTTAGTGCGTTACAATTACCTATCGCCCTCTATTGCGATTCAAAATGCGTTGGAGAAAAACCGCTTTAAGA
TAAAATAA

Upstream 100 bases:

>100_bases
TGTTGTTGCTACGCCCTATTTTAACAGATTTTACCACCAAAAATTCATGTTCTCATGCGTTGTGAAAAACCGAAGCTACG
CCATTTTTAAGTAAGCGTTC

Downstream 100 bases:

>100_bases
AAGGCTCTTTTTAACCCAAACTCCAAAAAAGGAGTCTTAAAGTTGCCGGATCTGTTGCATTAAGGCTAAATTTAGAGAAT
AGATTAGAAAAAGTCTGTTC

Product: cell division protein

Products: NA

Alternate protein names: PBP-2B; Penicillin-binding protein B [H]

Number of amino acids: Translated: 615; Mature: 615

Protein sequence:

>615_residues
MDNKNIDPSFNPERFLETQKYKGSVTALIFLLLFIVFLMVAFKKAFFAQANMPTLVMSKQDTATRGTIYSQDNYSLAASQ
TLFKLGFDTRFLNPDKEDFFVDFLSIYSNIPKKSLKDAINTKGYIILAYDLTPNMAANIRDLNKKFLTFGVFQNFKDAHD
KVWQKQGLNIEVSGVSRHYPYQNSLEPIIGYVQKQEEDKLTLTTGKKGVEKSQDHLLKAQQNGIRTGKRDVSFNFIQNHS
YTEVERLDGYEVYLSVPLKLQREIETLLDKTKDKLKAEEILVGIINPKSGEILSLASSKRFNPNAIKTSDYESLNLSVAE
KVFEPGSTIKPIVYSLLLDKNLINPKERIDLNHGYYQLGKYTIKDDFIPSKKAVMEDILIQSSNVGMIKISKNLNPEDFY
NGLLGYGFSQKTGIDLSLEATGKIPPLSAFKREVLKGSVSYGYGLNATFLQLLRAYAVFSNEGKLTTPYLVQRETAPNGD
IYIPSPKPTFQVISPKSARKMKETLIKVVRYGTGKNAQFEGLYIGGKTGTARVAKNGSYSVQSYNSSFFGFAEDERQVFT
IGVVILGSHGKEEYYASKIAAPIFKEITEILVRYNYLSPSIAIQNALEKNRFKIK

Sequences:

>Translated_615_residues
MDNKNIDPSFNPERFLETQKYKGSVTALIFLLLFIVFLMVAFKKAFFAQANMPTLVMSKQDTATRGTIYSQDNYSLAASQ
TLFKLGFDTRFLNPDKEDFFVDFLSIYSNIPKKSLKDAINTKGYIILAYDLTPNMAANIRDLNKKFLTFGVFQNFKDAHD
KVWQKQGLNIEVSGVSRHYPYQNSLEPIIGYVQKQEEDKLTLTTGKKGVEKSQDHLLKAQQNGIRTGKRDVSFNFIQNHS
YTEVERLDGYEVYLSVPLKLQREIETLLDKTKDKLKAEEILVGIINPKSGEILSLASSKRFNPNAIKTSDYESLNLSVAE
KVFEPGSTIKPIVYSLLLDKNLINPKERIDLNHGYYQLGKYTIKDDFIPSKKAVMEDILIQSSNVGMIKISKNLNPEDFY
NGLLGYGFSQKTGIDLSLEATGKIPPLSAFKREVLKGSVSYGYGLNATFLQLLRAYAVFSNEGKLTTPYLVQRETAPNGD
IYIPSPKPTFQVISPKSARKMKETLIKVVRYGTGKNAQFEGLYIGGKTGTARVAKNGSYSVQSYNSSFFGFAEDERQVFT
IGVVILGSHGKEEYYASKIAAPIFKEITEILVRYNYLSPSIAIQNALEKNRFKIK
>Mature_615_residues
MDNKNIDPSFNPERFLETQKYKGSVTALIFLLLFIVFLMVAFKKAFFAQANMPTLVMSKQDTATRGTIYSQDNYSLAASQ
TLFKLGFDTRFLNPDKEDFFVDFLSIYSNIPKKSLKDAINTKGYIILAYDLTPNMAANIRDLNKKFLTFGVFQNFKDAHD
KVWQKQGLNIEVSGVSRHYPYQNSLEPIIGYVQKQEEDKLTLTTGKKGVEKSQDHLLKAQQNGIRTGKRDVSFNFIQNHS
YTEVERLDGYEVYLSVPLKLQREIETLLDKTKDKLKAEEILVGIINPKSGEILSLASSKRFNPNAIKTSDYESLNLSVAE
KVFEPGSTIKPIVYSLLLDKNLINPKERIDLNHGYYQLGKYTIKDDFIPSKKAVMEDILIQSSNVGMIKISKNLNPEDFY
NGLLGYGFSQKTGIDLSLEATGKIPPLSAFKREVLKGSVSYGYGLNATFLQLLRAYAVFSNEGKLTTPYLVQRETAPNGD
IYIPSPKPTFQVISPKSARKMKETLIKVVRYGTGKNAQFEGLYIGGKTGTARVAKNGSYSVQSYNSSFFGFAEDERQVFT
IGVVILGSHGKEEYYASKIAAPIFKEITEILVRYNYLSPSIAIQNALEKNRFKIK

Specific function: Penicillin-binding proteins (PBPs) function in the late steps of murein biosynthesis. PBP-2B is required for vegetative cell division and sporulation septation. Beta-lactamase inactivates the PBPs by acylating an essential serine residue in the active sit

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PASTA domains [H]

Homologues:

Organism=Escherichia coli, GI1786272, Length=600, Percent_Identity=25.8333333333333, Blast_Score=134, Evalue=2e-32,
Organism=Escherichia coli, GI1786854, Length=479, Percent_Identity=25.4697286012526, Blast_Score=96, Evalue=8e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005543
- InterPro:   IPR005311
- InterPro:   IPR001460 [H]

Pfam domain/function: PF03793 PASTA; PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: NA

Molecular weight: Translated: 69366; Mature: 69366

Theoretical pI: Translated: 9.79; Mature: 9.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNKNIDPSFNPERFLETQKYKGSVTALIFLLLFIVFLMVAFKKAFFAQANMPTLVMSKQ
CCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECC
DTATRGTIYSQDNYSLAASQTLFKLGFDTRFLNPDKEDFFVDFLSIYSNIPKKSLKDAIN
CCCCCCEEEECCCCCCHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHCCCHHHHHHHHC
TKGYIILAYDLTPNMAANIRDLNKKFLTFGVFQNFKDAHDKVWQKQGLNIEVSGVSRHYP
CCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCC
YQNSLEPIIGYVQKQEEDKLTLTTGKKGVEKSQDHLLKAQQNGIRTGKRDVSFNFIQNHS
CCCCHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEECCCC
YTEVERLDGYEVYLSVPLKLQREIETLLDKTKDKLKAEEILVGIINPKSGEILSLASSKR
CCHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHCHHHEEEEEECCCCCCEEEECCCCC
FNPNAIKTSDYESLNLSVAEKVFEPGSTIKPIVYSLLLDKNLINPKERIDLNHGYYQLGK
CCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHEECCCCEEEECC
YTIKDDFIPSKKAVMEDILIQSSNVGMIKISKNLNPEDFYNGLLGYGFSQKTGIDLSLEA
EEECCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHCCCCCCCCCEEEEEC
TGKIPPLSAFKREVLKGSVSYGYGLNATFLQLLRAYAVFSNEGKLTTPYLVQRETAPNGD
CCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCC
IYIPSPKPTFQVISPKSARKMKETLIKVVRYGTGKNAQFEGLYIGGKTGTARVAKNGSYS
EECCCCCCCEEEECCHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCEEEECCCCEE
VQSYNSSFFGFAEDERQVFTIGVVILGSHGKEEYYASKIAAPIFKEITEILVRYNYLSPS
EEECCCCCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
IAIQNALEKNRFKIK
HHHHHHHHCCCEECC
>Mature Secondary Structure
MDNKNIDPSFNPERFLETQKYKGSVTALIFLLLFIVFLMVAFKKAFFAQANMPTLVMSKQ
CCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECC
DTATRGTIYSQDNYSLAASQTLFKLGFDTRFLNPDKEDFFVDFLSIYSNIPKKSLKDAIN
CCCCCCEEEECCCCCCHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHCCCHHHHHHHHC
TKGYIILAYDLTPNMAANIRDLNKKFLTFGVFQNFKDAHDKVWQKQGLNIEVSGVSRHYP
CCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCC
YQNSLEPIIGYVQKQEEDKLTLTTGKKGVEKSQDHLLKAQQNGIRTGKRDVSFNFIQNHS
CCCCHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEECCCC
YTEVERLDGYEVYLSVPLKLQREIETLLDKTKDKLKAEEILVGIINPKSGEILSLASSKR
CCHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHCHHHEEEEEECCCCCCEEEECCCCC
FNPNAIKTSDYESLNLSVAEKVFEPGSTIKPIVYSLLLDKNLINPKERIDLNHGYYQLGK
CCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHEECCCCEEEECC
YTIKDDFIPSKKAVMEDILIQSSNVGMIKISKNLNPEDFYNGLLGYGFSQKTGIDLSLEA
EEECCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHCCCCCCCCCEEEEEC
TGKIPPLSAFKREVLKGSVSYGYGLNATFLQLLRAYAVFSNEGKLTTPYLVQRETAPNGD
CCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCC
IYIPSPKPTFQVISPKSARKMKETLIKVVRYGTGKNAQFEGLYIGGKTGTARVAKNGSYS
EECCCCCCCEEEECCHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCEEEECCCCEE
VQSYNSSFFGFAEDERQVFTIGVVILGSHGKEEYYASKIAAPIFKEITEILVRYNYLSPS
EEECCCCCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
IAIQNALEKNRFKIK
HHHHHHHHCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8244929; 9384377; 8289242 [H]