Click here to switch to the map view.

The map label for this gene is murF [H]

Identifier: 108563149

GI number: 108563149

Start: 761996

End: 763477

Strand: Direct

Name: murF [H]

Synonym: HPAG1_0724

Alternate gene names: 108563149

Gene position: 761996-763477 (Clockwise)

Preceding gene: 108563148

Following gene: 108563150

Centisome position: 47.73

GC content: 38.6

Gene sequence:

>1482_bases
ATGCAAAGTCTTAGTTGGCTGAATTTAGCGTTCCGTTGGCTCTTTATAACAGGGCTTGGCTATTATATAATGACTTTATT
GCAATGGTATCATTACAGCGTGTTTCGGATCTTAACCAAGCACCACAAAATGCGTTGGCATGGGATTTATTTTTTATTGC
CTTTAGGGGTGTTTATCCTATCGTATGCTTTCAAAATGCCGTTTGTTTTTGATTTCTTTTGCGGCGTTATTCAAATGCCC
ATGCTCATTATTTGGGCCAAACGCAACGACAAGCCTTTAGTTTTCACGCCAAGGGTGAAGCGCTTTTTTATCTTCTTATT
ACTCTTTTTAATCTTGCATGAAATCTTAAATATAGAATTAGTCTCTTTGGATGGGATTTCGCTCGCACTAGGCTGTTTGT
GTTTGTTTATATTCGTTTTAAGCGCTTCTTTAATCTCTGAAAAAGCCTTATCCAAGCAGTATTTGCAAACCGCTAAAGAC
AAAATCGCCTCTTTAAAACATTTAAAAGTCATCGCCATTACCGGAAGCTTTGGAAAAACCAGCACCAAAAATTTCTTGCT
TCAAATCTTACAAACCACATTCAACGTGCATGCAAGCCCCAAAAGCGTCAACACCCTTTTAGGCATTGCGAACGATATTA
ACCAGAATTTAGATGATAAGAGCGAAATCTATATCGCTGAAGCCGGGGCAAGGAATAAGGGCGATATTAAAGAAATCACC
CGCCTTATTGAACCGCACCTTGCTGTGGTCGCAGAAGTGGGCGAACAGCATTTAGAATATTTTAAAACTTTAGAAAATAT
TTGCGAGACTAAAGCGGAATTATTGGATTCCAAACGCTTAGAAAAAGCCTTTTGTTACTCTGTGGAAAAAATCAAACCCT
ATGCCCCTAAAGATAGCCCTTTAATAGATGTTTCTAGTCTGGTTAAAAACATCCAATCCACTTTAAAAGGCACTTCTTTT
GAAATGCTTTTAGATAGCGTTTGGGAAAGATTTGAAACAAAGGTTCTAGGGGAGTTTAGCGCTTATAATATCGCTTCAGC
CATTTTAATCGCTAAGCATTTAGGCCTAGAGACAGAAAGGATCAAACGGCTTGTTTTGGAACTCAACCCCATTGCCCATC
GCTTGCAACTTTTGGAAGTGAATCAAAAAATCATCATAGACGATAGCTTTAATGGGAATTTAAAGGGCATGTTAGAGGGC
ATTCGTTTAGCGAGTTTGCACAAAGGGCGTAAAGTCATTGTAACACCGGGATTAGTGGAAAGCAACACAGAAAGTAATGA
AACTTTAGCGCAAAAAATAGATGGGGTTTTTGATGTCGCTATCATCACAGGGGAGTTGAATTCCAAAACGATTGCTTCAC
AATTGAAAACCCCTCAAAAAATCTTACTCAAGGATAAGGCGCAATTGGAAAATATCTTACAAGCCACCACGATTCAAGGC
GATTTGATTTTATTCGCTAATGACACCCCTAATTACATTTAG

Upstream 100 bases:

>100_bases
ACCTTATTGAAAAAGAGCGAATTATTCGTTTTAGAAGGGGATCATTTCTTTTTTTTAAACCAAGCAAAAGAGATTGAAAA
ACTAGTGGAGAATTACCATC

Downstream 100 bases:

>100_bases
GAAATGAACATGCAACATTTATACGCTCCTTGGCGCGAAAGTTATTTGAAAGAGAAAAATAAGAGTTGTGTCTTTTGCGA
GATTTCTCAAAACCCTACAA

Product: UDP-MurNac-pentapeptide presynthetase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 493; Mature: 493

Protein sequence:

>493_residues
MQSLSWLNLAFRWLFITGLGYYIMTLLQWYHYSVFRILTKHHKMRWHGIYFLLPLGVFILSYAFKMPFVFDFFCGVIQMP
MLIIWAKRNDKPLVFTPRVKRFFIFLLLFLILHEILNIELVSLDGISLALGCLCLFIFVLSASLISEKALSKQYLQTAKD
KIASLKHLKVIAITGSFGKTSTKNFLLQILQTTFNVHASPKSVNTLLGIANDINQNLDDKSEIYIAEAGARNKGDIKEIT
RLIEPHLAVVAEVGEQHLEYFKTLENICETKAELLDSKRLEKAFCYSVEKIKPYAPKDSPLIDVSSLVKNIQSTLKGTSF
EMLLDSVWERFETKVLGEFSAYNIASAILIAKHLGLETERIKRLVLELNPIAHRLQLLEVNQKIIIDDSFNGNLKGMLEG
IRLASLHKGRKVIVTPGLVESNTESNETLAQKIDGVFDVAIITGELNSKTIASQLKTPQKILLKDKAQLENILQATTIQG
DLILFANDTPNYI

Sequences:

>Translated_493_residues
MQSLSWLNLAFRWLFITGLGYYIMTLLQWYHYSVFRILTKHHKMRWHGIYFLLPLGVFILSYAFKMPFVFDFFCGVIQMP
MLIIWAKRNDKPLVFTPRVKRFFIFLLLFLILHEILNIELVSLDGISLALGCLCLFIFVLSASLISEKALSKQYLQTAKD
KIASLKHLKVIAITGSFGKTSTKNFLLQILQTTFNVHASPKSVNTLLGIANDINQNLDDKSEIYIAEAGARNKGDIKEIT
RLIEPHLAVVAEVGEQHLEYFKTLENICETKAELLDSKRLEKAFCYSVEKIKPYAPKDSPLIDVSSLVKNIQSTLKGTSF
EMLLDSVWERFETKVLGEFSAYNIASAILIAKHLGLETERIKRLVLELNPIAHRLQLLEVNQKIIIDDSFNGNLKGMLEG
IRLASLHKGRKVIVTPGLVESNTESNETLAQKIDGVFDVAIITGELNSKTIASQLKTPQKILLKDKAQLENILQATTIQG
DLILFANDTPNYI
>Mature_493_residues
MQSLSWLNLAFRWLFITGLGYYIMTLLQWYHYSVFRILTKHHKMRWHGIYFLLPLGVFILSYAFKMPFVFDFFCGVIQMP
MLIIWAKRNDKPLVFTPRVKRFFIFLLLFLILHEILNIELVSLDGISLALGCLCLFIFVLSASLISEKALSKQYLQTAKD
KIASLKHLKVIAITGSFGKTSTKNFLLQILQTTFNVHASPKSVNTLLGIANDINQNLDDKSEIYIAEAGARNKGDIKEIT
RLIEPHLAVVAEVGEQHLEYFKTLENICETKAELLDSKRLEKAFCYSVEKIKPYAPKDSPLIDVSSLVKNIQSTLKGTSF
EMLLDSVWERFETKVLGEFSAYNIASAILIAKHLGLETERIKRLVLELNPIAHRLQLLEVNQKIIIDDSFNGNLKGMLEG
IRLASLHKGRKVIVTPGLVESNTESNETLAQKIDGVFDVAIITGELNSKTIASQLKTPQKILLKDKAQLENILQATTIQG
DLILFANDTPNYI

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 55865; Mature: 55865

Theoretical pI: Translated: 9.15; Mature: 9.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSLSWLNLAFRWLFITGLGYYIMTLLQWYHYSVFRILTKHHKMRWHGIYFLLPLGVFIL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SYAFKMPFVFDFFCGVIQMPMLIIWAKRNDKPLVFTPRVKRFFIFLLLFLILHEILNIEL
HHHHHCHHHHHHHHHHHHHHHEEEEECCCCCCEEECCHHHHHHHHHHHHHHHHHHHCEEE
VSLDGISLALGCLCLFIFVLSASLISEKALSKQYLQTAKDKIASLKHLKVIAITGSFGKT
EEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCCC
STKNFLLQILQTTFNVHASPKSVNTLLGIANDINQNLDDKSEIYIAEAGARNKGDIKEIT
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCHHHHH
RLIEPHLAVVAEVGEQHLEYFKTLENICETKAELLDSKRLEKAFCYSVEKIKPYAPKDSP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
LIDVSSLVKNIQSTLKGTSFEMLLDSVWERFETKVLGEFSAYNIASAILIAKHLGLETER
CCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCHHH
IKRLVLELNPIAHRLQLLEVNQKIIIDDSFNGNLKGMLEGIRLASLHKGRKVIVTPGLVE
HHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCC
SNTESNETLAQKIDGVFDVAIITGELNSKTIASQLKTPQKILLKDKAQLENILQATTIQG
CCCCCHHHHHHHHCCCEEEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHEECC
DLILFANDTPNYI
CEEEEECCCCCCC
>Mature Secondary Structure
MQSLSWLNLAFRWLFITGLGYYIMTLLQWYHYSVFRILTKHHKMRWHGIYFLLPLGVFIL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SYAFKMPFVFDFFCGVIQMPMLIIWAKRNDKPLVFTPRVKRFFIFLLLFLILHEILNIEL
HHHHHCHHHHHHHHHHHHHHHEEEEECCCCCCEEECCHHHHHHHHHHHHHHHHHHHCEEE
VSLDGISLALGCLCLFIFVLSASLISEKALSKQYLQTAKDKIASLKHLKVIAITGSFGKT
EEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCCC
STKNFLLQILQTTFNVHASPKSVNTLLGIANDINQNLDDKSEIYIAEAGARNKGDIKEIT
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCHHHHH
RLIEPHLAVVAEVGEQHLEYFKTLENICETKAELLDSKRLEKAFCYSVEKIKPYAPKDSP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
LIDVSSLVKNIQSTLKGTSFEMLLDSVWERFETKVLGEFSAYNIASAILIAKHLGLETER
CCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCHHH
IKRLVLELNPIAHRLQLLEVNQKIIIDDSFNGNLKGMLEGIRLASLHKGRKVIVTPGLVE
HHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCC
SNTESNETLAQKIDGVFDVAIITGELNSKTIASQLKTPQKILLKDKAQLENILQATTIQG
CCCCCHHHHHHHHCCCEEEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHEECC
DLILFANDTPNYI
CEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]