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The map label for this gene is dapF

Identifier: 108562970

GI number: 108562970

Start: 563564

End: 564385

Strand: Reverse

Name: dapF

Synonym: HPAG1_0545

Alternate gene names: 108562970

Gene position: 564385-563564 (Counterclockwise)

Preceding gene: 108562972

Following gene: 108562969

Centisome position: 35.35

GC content: 38.93

Gene sequence:

>822_bases
ATGGTGTTTTACAAATATTCAGGGAGTGGGAATGATTTTTTAATCGTTCAAAGTTTCAAAAAAAAAGATTTTTCCAATTT
AGCCAAACAGGTGTGCCATAGGCATGAGGGTTTTGGGGCTGATGGGCTTGTGGTCGTCTTACCGAGTAAAGATTATGACT
ACGAATGGGATTTTTACAATTCAGACGGCTCTAAAGCTGGCATGTGTGGGAATGCGAGCCGTTGCGTGGGGTTATTTGCC
TACCAACATGCTATAGCCCCTAAAGAGCATGTTTTTTTAGCTGGAAAAAGAGAGATTTCTATTCGCATAGAAGAACCCAA
TATCATAGAGAGCAATCTCGGTAACTACAAAATCCTAGATGTAATACCCGCTTTAAGATGCGAAAAATTTTTTACCAATG
ACAGCGTTTTAGAAAATATCCCTACTTTCTACCTCATAGATACAGGAGTGCCGCATTTAGTGGGATTTGTGAAAAATAAA
GAGGGGTTAAATTCCCTTAACACGCTGGAATTAAGGGCTTTAAGGCATGAATTTAACGCTAATATTAACATCGCTTTTAT
AGAAAATAAAGAGACGATTTTTTTACAAACTTATGAAAGAGGGGTTGAAGATTTCACGCTAGCTTGCGGGACAGGCATGG
CGGCGGTTTTTATCGCCGCACGCATTTTTTATAACACGCCTAAAAAAGCCGCTCTCATCCCTAAAAGCAACGAATCTTTA
GAGCTTTCTTTAAAAAATGATGGAATTTTTTATAAAGGCGCGGTGCGTTATATTGGCATGAGTGTTTTGGGCATGAGGGT
TTTTGAAAATGGGTGTTTTTGA

Upstream 100 bases:

>100_bases
TAAACTGAACCAAAAATTATCAAATGTTAGCTAAAAAACGCTCTTTTAAGGATAAAAAACCTCTTTTAACTTATAATTAA
AATCAAAAAGGCTTATACAG

Downstream 100 bases:

>100_bases
TCATTATTTCTTGTAATAGCAATGATTTTGTCGTAATTAAACATAATGCGCTATCATTTCTAGGCTGTTTTTAGCAATAC
AAAAAATATCCTAAAGGAAT

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MVFYKYSGSGNDFLIVQSFKKKDFSNLAKQVCHRHEGFGADGLVVVLPSKDYDYEWDFYNSDGSKAGMCGNASRCVGLFA
YQHAIAPKEHVFLAGKREISIRIEEPNIIESNLGNYKILDVIPALRCEKFFTNDSVLENIPTFYLIDTGVPHLVGFVKNK
EGLNSLNTLELRALRHEFNANINIAFIENKETIFLQTYERGVEDFTLACGTGMAAVFIAARIFYNTPKKAALIPKSNESL
ELSLKNDGIFYKGAVRYIGMSVLGMRVFENGCF

Sequences:

>Translated_273_residues
MVFYKYSGSGNDFLIVQSFKKKDFSNLAKQVCHRHEGFGADGLVVVLPSKDYDYEWDFYNSDGSKAGMCGNASRCVGLFA
YQHAIAPKEHVFLAGKREISIRIEEPNIIESNLGNYKILDVIPALRCEKFFTNDSVLENIPTFYLIDTGVPHLVGFVKNK
EGLNSLNTLELRALRHEFNANINIAFIENKETIFLQTYERGVEDFTLACGTGMAAVFIAARIFYNTPKKAALIPKSNESL
ELSLKNDGIFYKGAVRYIGMSVLGMRVFENGCF
>Mature_273_residues
MVFYKYSGSGNDFLIVQSFKKKDFSNLAKQVCHRHEGFGADGLVVVLPSKDYDYEWDFYNSDGSKAGMCGNASRCVGLFA
YQHAIAPKEHVFLAGKREISIRIEEPNIIESNLGNYKILDVIPALRCEKFFTNDSVLENIPTFYLIDTGVPHLVGFVKNK
EGLNSLNTLELRALRHEFNANINIAFIENKETIFLQTYERGVEDFTLACGTGMAAVFIAARIFYNTPKKAALIPKSNESL
ELSLKNDGIFYKGAVRYIGMSVLGMRVFENGCF

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family

Homologues:

Organism=Escherichia coli, GI87082334, Length=226, Percent_Identity=29.2035398230088, Blast_Score=84, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPF_HELPH (Q1CTW0)

Other databases:

- EMBL:   CP000241
- RefSeq:   YP_627286.1
- ProteinModelPortal:   Q1CTW0
- SMR:   Q1CTW0
- STRING:   Q1CTW0
- GeneID:   4098162
- GenomeReviews:   CP000241_GR
- KEGG:   hpa:HPAG1_0545
- eggNOG:   COG0253
- HOGENOM:   HBG399442
- OMA:   SGNDFVI
- ProtClustDB:   PRK00450
- BioCyc:   HPYL357544:HPAG1_0545-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00197
- InterPro:   IPR001653
- InterPro:   IPR018510
- TIGRFAMs:   TIGR00652

Pfam domain/function: PF01678 DAP_epimerase

EC number: =5.1.1.7

Molecular weight: Translated: 30653; Mature: 30653

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: ACT_SITE 69-69 ACT_SITE 209-209

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVFYKYSGSGNDFLIVQSFKKKDFSNLAKQVCHRHEGFGADGLVVVLPSKDYDYEWDFYN
CEEEEEECCCCEEEEEECHHCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCEECEEEC
SDGSKAGMCGNASRCVGLFAYQHAIAPKEHVFLAGKREISIRIEEPNIIESNLGNYKILD
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCEEEEEEECCCCEECCCCCCEEEEE
VIPALRCEKFFTNDSVLENIPTFYLIDTGVPHLVGFVKNKEGLNSLNTLELRALRHEFNA
HHHHHHHHHHCCCCHHHHHCCEEEEEECCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHCC
NINIAFIENKETIFLQTYERGVEDFTLACGTGMAAVFIAARIFYNTPKKAALIPKSNESL
CEEEEEEECCCEEEEEEHHCCCCCEEEECCCCHHHHHHHHHHHHCCCCCEEEECCCCCCE
ELSLKNDGIFYKGAVRYIGMSVLGMRVFENGCF
EEEECCCCEEEECHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MVFYKYSGSGNDFLIVQSFKKKDFSNLAKQVCHRHEGFGADGLVVVLPSKDYDYEWDFYN
CEEEEEECCCCEEEEEECHHCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCEECEEEC
SDGSKAGMCGNASRCVGLFAYQHAIAPKEHVFLAGKREISIRIEEPNIIESNLGNYKILD
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCEEEEEEECCCCEECCCCCCEEEEE
VIPALRCEKFFTNDSVLENIPTFYLIDTGVPHLVGFVKNKEGLNSLNTLELRALRHEFNA
HHHHHHHHHHCCCCHHHHHCCEEEEEECCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHCC
NINIAFIENKETIFLQTYERGVEDFTLACGTGMAAVFIAARIFYNTPKKAALIPKSNESL
CEEEEEEECCCEEEEEEHHCCCCCEEEECCCCHHHHHHHHHHHHCCCCCEEEECCCCCCE
ELSLKNDGIFYKGAVRYIGMSVLGMRVFENGCF
EEEECCCCEEEECHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA