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The map label for this gene is ispB [H]

Identifier: 108562668

GI number: 108562668

Start: 248132

End: 249055

Strand: Reverse

Name: ispB [H]

Synonym: HPAG1_0243

Alternate gene names: 108562668

Gene position: 249055-248132 (Counterclockwise)

Preceding gene: 108562669

Following gene: 108562667

Centisome position: 15.6

GC content: 36.36

Gene sequence:

>924_bases
ATGCAAGAAAAACAACTTAAAACCATTCAAAATAAGATCGCTTCTTGGATCAAAGAAATAGAAAGCGGCTTTATAGATGC
ATTGTTTTCTAAGATTGGCCCCTCAAAAATGCTGCGCTCCAAACTCATGCTCGCTTTATTAGACGAAAAAACAGACGCTA
TTTTATTAGATAAAGCATTCAATTTGTGCGCGATTGTGGAAATGATACAGACCGCTTCTTTATTGCATGATGATGTGATT
GACAAAGCGACCATGCGCCGAAAACTCCCCAGCATTAACGCTCTTTTTGGGAATTTTAACGCCGTGATGCTTGGGGATGT
GTTTTATTCTAAAGCCTTTTTTGAACTCTCTAAAATGGGCGAAGTAATCGCTCAAGCTCTCTCTAATGCGGTTTTAAGGC
TCTCTAGAGGCGAGATTGAAGATGTGTTTGTGGGGGGAAGTTTTAATAGCGACAAACAAAAATACTGGTGCATTTTAGAA
GACAAGACCGCTCATTTCATAGAAGCGAGCTTAAAGAGCATGGCGATTCTTTTAAATAAAGACGCCAAAATGTATGCAGA
TTTTGGATTAAATTTTGGCATGGCATTTCAAATCATTGATGATTTATTAGACATCACTCAAGACGCCAAAACTTTAGGTA
AGCCCAATTTTAGCGATTTTAAAGAGGGCAAGACCACTTTACCCTACTTGCTTTTATATGAAAAATTAAATCAGCATGAT
CAAGGGCTTTTAATTTCTTATTTTAAACAAGATAGCCATGAAATCATAGAATGGACTAAGGAAAAATTCAAGCAACATGG
TATCATAGAAGAAACCCTTAAAATCGCTCAAGTTTATTCTAAAAAGGCCCTTGAAGCCATTAAAGGGGAAAACAATTTGA
TTTTAGAAAAACTAGCGCAAGATGTCATTTATAGGACTTTTTAA

Upstream 100 bases:

>100_bases
GTCGCTTGTGTTTAAAGATTTTAAAGAAGATAAAGCCGCTTTAAGGCTTTTAGCCCTTATGCCCCTTGTTTTTTCTCCTA
AAGAGCCTTAAGGAATTTGC

Downstream 100 bases:

>100_bases
TGGAGTTAGAAACTCATTTGTCAAAATATTTCACCCTAGCCTTTACGCATAAAAGCATGAGCTTAGAAATGCGAGAAAAA
CTCGCTATCAATTCAAGCGC

Product: putative octaprenyl-diphosphate synthase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MQEKQLKTIQNKIASWIKEIESGFIDALFSKIGPSKMLRSKLMLALLDEKTDAILLDKAFNLCAIVEMIQTASLLHDDVI
DKATMRRKLPSINALFGNFNAVMLGDVFYSKAFFELSKMGEVIAQALSNAVLRLSRGEIEDVFVGGSFNSDKQKYWCILE
DKTAHFIEASLKSMAILLNKDAKMYADFGLNFGMAFQIIDDLLDITQDAKTLGKPNFSDFKEGKTTLPYLLLYEKLNQHD
QGLLISYFKQDSHEIIEWTKEKFKQHGIIEETLKIAQVYSKKALEAIKGENNLILEKLAQDVIYRTF

Sequences:

>Translated_307_residues
MQEKQLKTIQNKIASWIKEIESGFIDALFSKIGPSKMLRSKLMLALLDEKTDAILLDKAFNLCAIVEMIQTASLLHDDVI
DKATMRRKLPSINALFGNFNAVMLGDVFYSKAFFELSKMGEVIAQALSNAVLRLSRGEIEDVFVGGSFNSDKQKYWCILE
DKTAHFIEASLKSMAILLNKDAKMYADFGLNFGMAFQIIDDLLDITQDAKTLGKPNFSDFKEGKTTLPYLLLYEKLNQHD
QGLLISYFKQDSHEIIEWTKEKFKQHGIIEETLKIAQVYSKKALEAIKGENNLILEKLAQDVIYRTF
>Mature_307_residues
MQEKQLKTIQNKIASWIKEIESGFIDALFSKIGPSKMLRSKLMLALLDEKTDAILLDKAFNLCAIVEMIQTASLLHDDVI
DKATMRRKLPSINALFGNFNAVMLGDVFYSKAFFELSKMGEVIAQALSNAVLRLSRGEIEDVFVGGSFNSDKQKYWCILE
DKTAHFIEASLKSMAILLNKDAKMYADFGLNFGMAFQIIDDLLDITQDAKTLGKPNFSDFKEGKTTLPYLLLYEKLNQHD
QGLLISYFKQDSHEIIEWTKEKFKQHGIIEETLKIAQVYSKKALEAIKGENNLILEKLAQDVIYRTF

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=230, Percent_Identity=31.7391304347826, Blast_Score=109, Evalue=3e-24,
Organism=Escherichia coli, GI1789578, Length=255, Percent_Identity=30.9803921568627, Blast_Score=128, Evalue=4e-31,
Organism=Escherichia coli, GI1786623, Length=240, Percent_Identity=27.9166666666667, Blast_Score=66, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17505681, Length=234, Percent_Identity=32.9059829059829, Blast_Score=107, Evalue=8e-24,
Organism=Saccharomyces cerevisiae, GI6319475, Length=282, Percent_Identity=29.7872340425532, Blast_Score=106, Evalue=4e-24,
Organism=Drosophila melanogaster, GI24651612, Length=188, Percent_Identity=33.5106382978723, Blast_Score=101, Evalue=7e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 34917; Mature: 34917

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQEKQLKTIQNKIASWIKEIESGFIDALFSKIGPSKMLRSKLMLALLDEKTDAILLDKAF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEHHHHHH
NLCAIVEMIQTASLLHDDVIDKATMRRKLPSINALFGNFNAVMLGDVFYSKAFFELSKMG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCEEEHHHHHHHHHHHHHHHH
EVIAQALSNAVLRLSRGEIEDVFVGGSFNSDKQKYWCILEDKTAHFIEASLKSMAILLNK
HHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHCC
DAKMYADFGLNFGMAFQIIDDLLDITQDAKTLGKPNFSDFKEGKTTLPYLLLYEKLNQHD
CHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCHHHHHHHHHHHCCCC
QGLLISYFKQDSHEIIEWTKEKFKQHGIIEETLKIAQVYSKKALEAIKGENNLILEKLAQ
CCHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
DVIYRTF
HHHHHCC
>Mature Secondary Structure
MQEKQLKTIQNKIASWIKEIESGFIDALFSKIGPSKMLRSKLMLALLDEKTDAILLDKAF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEHHHHHH
NLCAIVEMIQTASLLHDDVIDKATMRRKLPSINALFGNFNAVMLGDVFYSKAFFELSKMG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCEEEHHHHHHHHHHHHHHHH
EVIAQALSNAVLRLSRGEIEDVFVGGSFNSDKQKYWCILEDKTAHFIEASLKSMAILLNK
HHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHCC
DAKMYADFGLNFGMAFQIIDDLLDITQDAKTLGKPNFSDFKEGKTTLPYLLLYEKLNQHD
CHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCHHHHHHHHHHHCCCC
QGLLISYFKQDSHEIIEWTKEKFKQHGIIEETLKIAQVYSKKALEAIKGENNLILEKLAQ
CCHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
DVIYRTF
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8312607; 9278503; 2670911; 8037730 [H]