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The map label for this gene is ecaIM [H]

Identifier: 108562473

GI number: 108562473

Start: 51621

End: 52652

Strand: Reverse

Name: ecaIM [H]

Synonym: HPAG1_0048

Alternate gene names: 108562473

Gene position: 52652-51621 (Counterclockwise)

Preceding gene: 108562474

Following gene: 108562472

Centisome position: 3.3

GC content: 32.46

Gene sequence:

>1032_bases
ATGCAAAACTTATTAATACAAGCAGAAAATGCAATCGCTCTACTTTTTTTATTGAATGATAAAAACCTAAAAGGAAAAAT
AGATTTGATATATATTGACCCTCCATTTGCTACAAACAACCATTTTACTATCACAAATGGCAGAGCAACCACAATTAGCA
ATTCTAAGAATGGCGATATTGCTTATAGCGACAAAGTAGTAGGTATGGATTTTATAGAATTTTTAAAACAACGCCTGGTA
TTGCTTAAAGAATTGCTTTCAGAACAAGGCTCTATCTATGTGCATACAGATTGTAAGATAGGGCATTATGTTAAGGTAAT
GTTAGATGAAATATTTGGCATACAAAATTTTAGAAATGAAATCACACGCATAAAGTGCAATCCTAAAAATTTTAAAAGGA
TGGGCTATGGCAACATAAAAGATATGATTTTATTTTACTCTAAAGGAAAAAATCCCATTTTTAACGAACCTAAGATTCCT
TATACTCCACAAGATTTAGAAAAACGATTCCCTAAAATTGACAAAGATAAAAGGCGTTACACTACCGTTCCAATACATGC
TCCAGGAGAAGTAGAAAGTGGCGAATGTTCTAAAGCATTTAAAGGTATGCTACCTCCAAAAGGGCGGTATTGGCGCACTG
ATGTTGCCACACTTGAGCGTTGGGACAAAGAAGGTTTGATTGAGTACTCTAACAATAATAACCCTAGAAAAAAAATTTAT
GCCTTAGAACAAGTTGGCAAAAGAGTCCAAGACATCTGGGAATTTAAAGACCCACAATATCCAAGCTACCCTACAGAAAA
AAACGCTCAATTATTAGATTTAATCATTAAAACCTCTTCTAATAAAGATAGCATTGTTTTAGATTGTTTTTGTGGTTCTG
GAACAACCTTAAAATCTGCGTTTTTATTGCAACGAAAATTTATAGGCATTGATAATTCCTATTTGGCTATCCAAGCTTGC
AAAAACAAGCTTGAAACAATAACAAAAGACTTGTTTGTTTCTCAAAATTTTTATGATTTCCTTGTTTTTTAA

Upstream 100 bases:

>100_bases
TAATAAAATGCGTAAATTGCTAGATACTACTTATAGAAACTATAATATTATGAGCGCGTTAGTGTTACGAGAATTTTTAT
ATCAAATTTAAATGAGAGAA

Downstream 100 bases:

>100_bases
AAAGGTTTATGAAAGCTTGAAATGAATTATAAAATTTTAGATTTGTTTTGTGGGGCTGGGGGTTTTAGCGCTGGGTTAGA
GCGTTTAGAAGAGTTTGACG

Product: type II DNA modification enzyme

Products: NA

Alternate protein names: M.EcaI; Adenine-specific methyltransferase EcaI [H]

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MQNLLIQAENAIALLFLLNDKNLKGKIDLIYIDPPFATNNHFTITNGRATTISNSKNGDIAYSDKVVGMDFIEFLKQRLV
LLKELLSEQGSIYVHTDCKIGHYVKVMLDEIFGIQNFRNEITRIKCNPKNFKRMGYGNIKDMILFYSKGKNPIFNEPKIP
YTPQDLEKRFPKIDKDKRRYTTVPIHAPGEVESGECSKAFKGMLPPKGRYWRTDVATLERWDKEGLIEYSNNNNPRKKIY
ALEQVGKRVQDIWEFKDPQYPSYPTEKNAQLLDLIIKTSSNKDSIVLDCFCGSGTTLKSAFLLQRKFIGIDNSYLAIQAC
KNKLETITKDLFVSQNFYDFLVF

Sequences:

>Translated_343_residues
MQNLLIQAENAIALLFLLNDKNLKGKIDLIYIDPPFATNNHFTITNGRATTISNSKNGDIAYSDKVVGMDFIEFLKQRLV
LLKELLSEQGSIYVHTDCKIGHYVKVMLDEIFGIQNFRNEITRIKCNPKNFKRMGYGNIKDMILFYSKGKNPIFNEPKIP
YTPQDLEKRFPKIDKDKRRYTTVPIHAPGEVESGECSKAFKGMLPPKGRYWRTDVATLERWDKEGLIEYSNNNNPRKKIY
ALEQVGKRVQDIWEFKDPQYPSYPTEKNAQLLDLIIKTSSNKDSIVLDCFCGSGTTLKSAFLLQRKFIGIDNSYLAIQAC
KNKLETITKDLFVSQNFYDFLVF
>Mature_343_residues
MQNLLIQAENAIALLFLLNDKNLKGKIDLIYIDPPFATNNHFTITNGRATTISNSKNGDIAYSDKVVGMDFIEFLKQRLV
LLKELLSEQGSIYVHTDCKIGHYVKVMLDEIFGIQNFRNEITRIKCNPKNFKRMGYGNIKDMILFYSKGKNPIFNEPKIP
YTPQDLEKRFPKIDKDKRRYTTVPIHAPGEVESGECSKAFKGMLPPKGRYWRTDVATLERWDKEGLIEYSNNNNPRKKIY
ALEQVGKRVQDIWEFKDPQYPSYPTEKNAQLLDLIIKTSSNKDSIVLDCFCGSGTTLKSAFLLQRKFIGIDNSYLAIQAC
KNKLETITKDLFVSQNFYDFLVF

Specific function: This methylase recognizes the double-stranded sequence GGTNACC, causes specific methylation on A-5 on both strands and protects the DNA from cleavage by the EcaI endonuclease [H]

COG id: COG2189

COG function: function code L; Adenine specific DNA methylase Mod

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002295
- InterPro:   IPR002941
- InterPro:   IPR002052 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 39548; Mature: 39548

Theoretical pI: Translated: 9.39; Mature: 9.39

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNLLIQAENAIALLFLLNDKNLKGKIDLIYIDPPFATNNHFTITNGRATTISNSKNGDI
CCCCEEEECCEEEEEEEEECCCCCCEEEEEEECCCCCCCCEEEEECCEEEEECCCCCCCE
AYSDKVVGMDFIEFLKQRLVLLKELLSEQGSIYVHTDCKIGHYVKVMLDEIFGIQNFRNE
EECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCC
ITRIKCNPKNFKRMGYGNIKDMILFYSKGKNPIFNEPKIPYTPQDLEKRFPKIDKDKRRY
CEEEEECHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCEE
TTVPIHAPGEVESGECSKAFKGMLPPKGRYWRTDVATLERWDKEGLIEYSNNNNPRKKIY
EEEEEECCCCCCCCCHHHHHHCCCCCCCCEEECHHHHHHHCCCCCCEEECCCCCHHHHHH
ALEQVGKRVQDIWEFKDPQYPSYPTEKNAQLLDLIIKTSSNKDSIVLDCFCGSGTTLKSA
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEEECCCCCHHHHH
FLLQRKFIGIDNSYLAIQACKNKLETITKDLFVSQNFYDFLVF
HHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHCCCHHHEEEC
>Mature Secondary Structure
MQNLLIQAENAIALLFLLNDKNLKGKIDLIYIDPPFATNNHFTITNGRATTISNSKNGDI
CCCCEEEECCEEEEEEEEECCCCCCEEEEEEECCCCCCCCEEEEECCEEEEECCCCCCCE
AYSDKVVGMDFIEFLKQRLVLLKELLSEQGSIYVHTDCKIGHYVKVMLDEIFGIQNFRNE
EECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCC
ITRIKCNPKNFKRMGYGNIKDMILFYSKGKNPIFNEPKIPYTPQDLEKRFPKIDKDKRRY
CEEEEECHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCEE
TTVPIHAPGEVESGECSKAFKGMLPPKGRYWRTDVATLERWDKEGLIEYSNNNNPRKKIY
EEEEEECCCCCCCCCHHHHHHCCCCCCCCEEECHHHHHHHCCCCCCEEECCCCCHHHHHH
ALEQVGKRVQDIWEFKDPQYPSYPTEKNAQLLDLIIKTSSNKDSIVLDCFCGSGTTLKSA
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEEECCCCCHHHHH
FLLQRKFIGIDNSYLAIQACKNKLETITKDLFVSQNFYDFLVF
HHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHCCCHHHEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2183182 [H]