Click here to switch to the map view.

The map label for this gene is ddeIM [H]

Identifier: 108562472

GI number: 108562472

Start: 50532

End: 51599

Strand: Reverse

Name: ddeIM [H]

Synonym: HPAG1_0047

Alternate gene names: 108562472

Gene position: 51599-50532 (Counterclockwise)

Preceding gene: 108562473

Following gene: 108562471

Centisome position: 3.23

GC content: 39.51

Gene sequence:

>1068_bases
ATGAATTATAAAATTTTAGATTTGTTTTGTGGGGCTGGGGGTTTTAGCGCTGGGTTAGAGCGTTTAGAAGAGTTTGACGC
TTTAATAGGGCTAGATTGCGACAAACAAGCCCTAATCACTTTTGAAAACAACCATAAAAACGCTATAGGCGTTTGTGGGG
ACATCACTCAAACCGAAATTAAAGAAAAAGTCATCAAACTAGCTAAAAAATTAGAAATCAACATGATCATTGGCGGGCCT
CCATGTCAAGGCTTTTCTAATAAAGGGAAAAATCTAGGGCTAAAAGACCCTAGGAATTTTTTATTCTTAGAATATATAGA
AATAGTCAAAGCCATAAAGCCAGAAATTTTTATCATTGAAAACGTGAAAAATCTCATCTCTTGCGCTAAAGGCTATTTTT
TAGAAGAAATTAAAGAAAGGTTGAACGCTTTAGGGTATCAATTGAGCTATCAAATCCTAAACGCTAAAGATTATGGCGTG
CCTCAAAACAGAGAGAGGGCTTTTATTGTGGGGGCTAGTCGTTTCAGTTTTGATTTCAAGCTTTTAGAGCCTTCTCAAAG
CGTGAATGTTCAAGATGCGATAAGCGATTTAGCCTATCTTTGTTCTAATGAGGGGGCGTTTGAGAGCGATTATTTAAACC
ATATCCAATCAAGCTATCAAGCCTTGATGCGAAAAAATAGCCCTAAATTATACAACCACAAAGCCACCAACCACTCGCAA
GCCGCTTTAGAGAAATTAAAGCTCATCAATAAAGAGCAAGGCAAAGAATGCTTGCCTAAAAACTTGCATGGCAAACAGCA
ATTCAAAAGCACATGGGGGCACCTGAATTGGAATAAAATCAGCCCCACCATAGACACACGCTTTGACACTCCCAGTAATG
GCACCAACTCCCACCCTGAATTGCACCGCTCTATCACGCCTAGAGAAGCCGCCAGGATACAGAGTTTTAGCGATGATTAT
ATCTTTTATGGCAATAAAACGAGCGTTTGCAAGCAAATCGGTAACGCTGTGCCTCCTCTTCTAGCCCTAGCTTTAGGCAA
AGCGATCTTAAAAAGCTTAAGAAAATGA

Upstream 100 bases:

>100_bases
AGCTTGCAAAAACAAGCTTGAAACAATAACAAAAGACTTGTTTGTTTCTCAAAATTTTTATGATTTCCTTGTTTTTTAAA
AAGGTTTATGAAAGCTTGAA

Downstream 100 bases:

>100_bases
TACAAATTTATCACGCTGACGCTTTTGAAATCATCAAGGATTTTTACCAGCAAAATTTAAAAGTGGATGCGATCATCACG
GATCCTCCTTACAACATTTC

Product: cytosine specific DNA methyltransferase

Products: NA

Alternate protein names: M.DdeI; Cytosine-specific methyltransferase DdeI [H]

Number of amino acids: Translated: 355; Mature: 355

Protein sequence:

>355_residues
MNYKILDLFCGAGGFSAGLERLEEFDALIGLDCDKQALITFENNHKNAIGVCGDITQTEIKEKVIKLAKKLEINMIIGGP
PCQGFSNKGKNLGLKDPRNFLFLEYIEIVKAIKPEIFIIENVKNLISCAKGYFLEEIKERLNALGYQLSYQILNAKDYGV
PQNRERAFIVGASRFSFDFKLLEPSQSVNVQDAISDLAYLCSNEGAFESDYLNHIQSSYQALMRKNSPKLYNHKATNHSQ
AALEKLKLINKEQGKECLPKNLHGKQQFKSTWGHLNWNKISPTIDTRFDTPSNGTNSHPELHRSITPREAARIQSFSDDY
IFYGNKTSVCKQIGNAVPPLLALALGKAILKSLRK

Sequences:

>Translated_355_residues
MNYKILDLFCGAGGFSAGLERLEEFDALIGLDCDKQALITFENNHKNAIGVCGDITQTEIKEKVIKLAKKLEINMIIGGP
PCQGFSNKGKNLGLKDPRNFLFLEYIEIVKAIKPEIFIIENVKNLISCAKGYFLEEIKERLNALGYQLSYQILNAKDYGV
PQNRERAFIVGASRFSFDFKLLEPSQSVNVQDAISDLAYLCSNEGAFESDYLNHIQSSYQALMRKNSPKLYNHKATNHSQ
AALEKLKLINKEQGKECLPKNLHGKQQFKSTWGHLNWNKISPTIDTRFDTPSNGTNSHPELHRSITPREAARIQSFSDDY
IFYGNKTSVCKQIGNAVPPLLALALGKAILKSLRK
>Mature_355_residues
MNYKILDLFCGAGGFSAGLERLEEFDALIGLDCDKQALITFENNHKNAIGVCGDITQTEIKEKVIKLAKKLEINMIIGGP
PCQGFSNKGKNLGLKDPRNFLFLEYIEIVKAIKPEIFIIENVKNLISCAKGYFLEEIKERLNALGYQLSYQILNAKDYGV
PQNRERAFIVGASRFSFDFKLLEPSQSVNVQDAISDLAYLCSNEGAFESDYLNHIQSSYQALMRKNSPKLYNHKATNHSQ
AALEKLKLINKEQGKECLPKNLHGKQQFKSTWGHLNWNKISPTIDTRFDTPSNGTNSHPELHRSITPREAARIQSFSDDY
IFYGNKTSVCKQIGNAVPPLLALALGKAILKSLRK

Specific function: This methylase recognizes the double-stranded sequence CTNAG, causes specific methylation on C-1 on both strands, and protects the DNA from cleavage by the DdeI endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI195927037, Length=289, Percent_Identity=24.9134948096886, Blast_Score=96, Evalue=7e-20,
Organism=Homo sapiens, GI4503351, Length=289, Percent_Identity=24.9134948096886, Blast_Score=96, Evalue=7e-20,
Organism=Escherichia coli, GI1788271, Length=361, Percent_Identity=24.6537396121884, Blast_Score=78, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 39880; Mature: 39880

Theoretical pI: Translated: 8.97; Mature: 8.97

Prosite motif: PS00094 C5_MTASE_1 ; PS00095 C5_MTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYKILDLFCGAGGFSAGLERLEEFDALIGLDCDKQALITFENNHKNAIGVCGDITQTEI
CCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEECCCCHHHHH
KEKVIKLAKKLEINMIIGGPPCQGFSNKGKNLGLKDPRNFLFLEYIEIVKAIKPEIFIIE
HHHHHHHHHHCEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEHH
NVKNLISCAKGYFLEEIKERLNALGYQLSYQILNAKDYGVPQNRERAFIVGASRFSFDFK
HHHHHHHHHHHHHHHHHHHHHHHHCHHHEEEEECCCCCCCCCCCCCEEEEECCEEEEEEE
LLEPSQSVNVQDAISDLAYLCSNEGAFESDYLNHIQSSYQALMRKNSPKLYNHKATNHSQ
EECCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHH
AALEKLKLINKEQGKECLPKNLHGKQQFKSTWGHLNWNKISPTIDTRFDTPSNGTNSHPE
HHHHHHHHHHHHCCHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCH
LHRSITPREAARIQSFSDDYIFYGNKTSVCKQIGNAVPPLLALALGKAILKSLRK
HHHCCCHHHHHHHHCCCCCEEEECCCHHHHHHHCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNYKILDLFCGAGGFSAGLERLEEFDALIGLDCDKQALITFENNHKNAIGVCGDITQTEI
CCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEECCCCHHHHH
KEKVIKLAKKLEINMIIGGPPCQGFSNKGKNLGLKDPRNFLFLEYIEIVKAIKPEIFIIE
HHHHHHHHHHCEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEHH
NVKNLISCAKGYFLEEIKERLNALGYQLSYQILNAKDYGVPQNRERAFIVGASRFSFDFK
HHHHHHHHHHHHHHHHHHHHHHHHCHHHEEEEECCCCCCCCCCCCCEEEEECCEEEEEEE
LLEPSQSVNVQDAISDLAYLCSNEGAFESDYLNHIQSSYQALMRKNSPKLYNHKATNHSQ
EECCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHH
AALEKLKLINKEQGKECLPKNLHGKQQFKSTWGHLNWNKISPTIDTRFDTPSNGTNSHPE
HHHHHHHHHHHHCCHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCH
LHRSITPREAARIQSFSDDYIFYGNKTSVCKQIGNAVPPLLALALGKAILKSLRK
HHHCCCHHHHHHHHCCCCCEEEECCCHHHHHHHCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2823226 [H]