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The map label for this gene is hisH [H]

Identifier: 103488623

GI number: 103488623

Start: 3299163

End: 3299771

Strand: Reverse

Name: hisH [H]

Synonym: Sala_3148

Alternate gene names: 103488623

Gene position: 3299771-3299163 (Counterclockwise)

Preceding gene: 103488624

Following gene: 103488622

Centisome position: 98.64

GC content: 67.82

Gene sequence:

>609_bases
ATGAGCGTGATTGCCCTGATCGACTATGGCGCGGGCAATCTTCGCTCGGTGCATAATGCGCTCGTCGCGGCGGGCGCGGA
CACTGTCGCCGTCACCGCCGACCCCGATGCCGTGGCCCGGGCCGACCGCATCGTGCTGCCCGGCGTCGGCGCGTTCGCGG
CGTGCATGAACGGGCTGTCGGCGATCGACGGGCTGATCGAAGCGATGGTGCGCCGGGTGCGCGGCGAAGGTGCGCCCTTC
CTCGGCATCTGCGTCGGGATGCAATTGCTCGCCGATTCGGGCGAAGAGCATGGTCGGCATCCCGGCCTCGGCTGGATCGG
CGGCACGGTGCGCGCGTTCGACCCGCAACCGGGCCTCTGCATCCCGCACATGGGCTGGAACGATGTCGTCCCGACGTTCG
CGCACCCGGTGATCGCGGTGGGCGAAGCCTATTATCTCCACGGCTATCATTTCGCGGACGCGGCGCATGTCGCCGCGACG
AGCAGTCACGGCGGTCCCTTCACCGCCGCCGTGGCGAAGGACAATGTCGTGGGTGTCCAGTTTCATCCCGAAAAAAGCCA
GGCCTATGGCCTCGCGACGCTCGAAAGGTTCCTCGCTTGGCGGCCCTGA

Upstream 100 bases:

>100_bases
GTACAAGGCGCTGGCCAGGGCGCTGCGCCAGGCGGTCGAGATCGATCCGCGCAAGGGCGATGCGATTCCCTCGACCAAGG
GCGTGCTCTAGGAGCCGTCC

Downstream 100 bases:

>100_bases
CCATCTTCCCCGCGATCGACCTCAAGGCCGGACAGGTGGTGCGGCTGGCCGAGGGCGATATGGACCGCGCGACCGTCTAT
GGCGACGATCCGGCGGCGCA

Product: imidazole glycerol phosphate synthase subunit HisH

Products: AMP; diphosphate; GMP; L-glutamate

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 202; Mature: 201

Protein sequence:

>202_residues
MSVIALIDYGAGNLRSVHNALVAAGADTVAVTADPDAVARADRIVLPGVGAFAACMNGLSAIDGLIEAMVRRVRGEGAPF
LGICVGMQLLADSGEEHGRHPGLGWIGGTVRAFDPQPGLCIPHMGWNDVVPTFAHPVIAVGEAYYLHGYHFADAAHVAAT
SSHGGPFTAAVAKDNVVGVQFHPEKSQAYGLATLERFLAWRP

Sequences:

>Translated_202_residues
MSVIALIDYGAGNLRSVHNALVAAGADTVAVTADPDAVARADRIVLPGVGAFAACMNGLSAIDGLIEAMVRRVRGEGAPF
LGICVGMQLLADSGEEHGRHPGLGWIGGTVRAFDPQPGLCIPHMGWNDVVPTFAHPVIAVGEAYYLHGYHFADAAHVAAT
SSHGGPFTAAVAKDNVVGVQFHPEKSQAYGLATLERFLAWRP
>Mature_201_residues
SVIALIDYGAGNLRSVHNALVAAGADTVAVTADPDAVARADRIVLPGVGAFAACMNGLSAIDGLIEAMVRRVRGEGAPFL
GICVGMQLLADSGEEHGRHPGLGWIGGTVRAFDPQPGLCIPHMGWNDVVPTFAHPVIAVGEAYYLHGYHFADAAHVAATS
SHGGPFTAAVAKDNVVGVQFHPEKSQAYGLATLERFLAWRP

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=199, Percent_Identity=38.1909547738693, Blast_Score=118, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6319725, Length=216, Percent_Identity=30.0925925925926, Blast_Score=99, Evalue=7e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 6.3.5.2

Molecular weight: Translated: 21030; Mature: 20899

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVIALIDYGAGNLRSVHNALVAAGADTVAVTADPDAVARADRIVLPGVGAFAACMNGLS
CEEEEEEECCCCCHHHHHHHHHHCCCCEEEEECCCHHHHHCCEEEECCCHHHHHHHHHHH
AIDGLIEAMVRRVRGEGAPFLGICVGMQLLADSGEEHGRHPGLGWIGGTVRAFDPQPGLC
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHCCCCCCCEECCCEEEEECCCCCEE
IPHMGWNDVVPTFAHPVIAVGEAYYLHGYHFADAAHVAATSSHGGPFTAAVAKDNVVGVQ
ECCCCCCCCCHHHHCHHHHHCCEEEEECEECCCHHHHEEECCCCCCEEEEEECCCEEEEE
FHPEKSQAYGLATLERFLAWRP
ECCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SVIALIDYGAGNLRSVHNALVAAGADTVAVTADPDAVARADRIVLPGVGAFAACMNGLS
EEEEEEECCCCCHHHHHHHHHHCCCCEEEEECCCHHHHHCCEEEECCCHHHHHHHHHHH
AIDGLIEAMVRRVRGEGAPFLGICVGMQLLADSGEEHGRHPGLGWIGGTVRAFDPQPGLC
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHCCCCCCCEECCCEEEEECCCCCEE
IPHMGWNDVVPTFAHPVIAVGEAYYLHGYHFADAAHVAATSSHGGPFTAAVAKDNVVGVQ
ECCCCCCCCCHHHHCHHHHHCCEEEEECEECCCHHHHEEECCCCCCEEEEEECCCEEEEE
FHPEKSQAYGLATLERFLAWRP
ECCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; xanthosine 5'-phosphate; L-glutamine; H2O

Specific reaction: ATP + xanthosine 5'-phosphate + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA