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The map label for this gene is purD [H]

Identifier: 103488580

GI number: 103488580

Start: 3252053

End: 3253288

Strand: Reverse

Name: purD [H]

Synonym: Sala_3104

Alternate gene names: 103488580

Gene position: 3253288-3252053 (Counterclockwise)

Preceding gene: 103488585

Following gene: 103488579

Centisome position: 97.25

GC content: 70.15

Gene sequence:

>1236_bases
TTGAGCTGGCAACTGGCACAATCGCCAAGCTGCGCCAAGCTCTATGCCGCGCCGGGCAACCCGGGAATCGAACTCCACGC
CGAATGCGTGCCGATCGCCGCCGACGATCTCGATGGCCTGATCGTCTTCGTGCGCGCGCACGCGATCGACTTCGTCGTCG
TCGGTCCCGAAGCGCCGCTCGTCGCGGGCCTTGCCGACCGGCTGCGCGCCATCGGCGTGCCGGTCTTCGGTCCCTCGGCC
GCCGCCGCGCGGCTCGAAGGGTCGAAGGGTTTTACCAAGGATCTGTGTGCGCGCGCGTCGATTCCGACCGCCGCCTATGT
CCGCTGCACCAGCGCCGACGAGGCGCTCGCCGCGCTGGACGGCTTCGACGTTCCGGTCGTGATCAAGGCCGATGGCCTTG
CCGCGGGCAAGGGCGTCATCATCGCCGAAACGCGCGCCGAGGCCGAGGCGGCGATCGCCGACATGTTCGCCGGCGCCTTT
GGCGGCGCGGGCGCCGAGGTGGTGATCGAGGAGTTTATGACCGGCGAGGAGGCGAGCTTCTTCGCGCTTTCCGACGGGCA
GGACGTGATCGCCTTCGGCAGCGCGCAGGACCACAAGCGCGTCGGCGACGGCGACACCGGCCCGAACACCGGCGGCATGG
GCGCCTACAGCCCCGCCCCCGTGCTCACCCCCGATCTCGAAGTCGCGGTGATGGAGCGCATCATCCGCCCGACGGTGGGG
ACACTCGCCGCCGAAGCCACACCCTATGTCGGCGTGCTCTTTGCCGGACTGATGCTCACTGCCGAAGGGCCGAAGCTGAT
CGAATATAATTGCCGTTTCGGCGATCCCGAATGCCAGGTGCTGATGATGCGCTTCCGCGGCGATCTTGCCGCGCTGCTCC
ACGCCGCCGCGACGGGCCGACTGGCCCGCGCCGAGCCGCCAACCTTTGCGCATGACTATGCGCTCACCGTCGTCATGGCC
GCCAAGGGTTATCCGGGCACCCCCGAAAAGGGCGGCGCGATCCGCCGTATCGCCGATGCCGAAGTCGGCGGCGTGCGCGT
GTTCCACGCCGGCACCGCGCGCGAGGATCGCACGCTCGTCGCCGCGGGCGGCCGCGTGCTCAACGTCACCGCGACGGGCA
AGAGCGTCACCGAGGCGCAGGCGCGCGCCTATGCCGCGCTCGACCGGATCGACTTTCCGACCGGCTTCTGCCGCCGCGAC
ATCGGCTGGCGCGAGGTCGCGCGCGAGGCGCGCTGA

Upstream 100 bases:

>100_bases
GGCTAACAGCCGCGCGTCGGTGCCGTCGCCGGGCGCCTCATCGGGAAAAGGAACTGCCATGAATATCCTGCTCATCGGGT
CGGGGGGCCGCGAACATGCG

Downstream 100 bases:

>100_bases
GGGGCTGGCGCGAGGCCGCGCGCGGGGCCGAATCGCGTCGCTCGTCCCACCCGGCAGAGCAAGGGCGGGACTGGACGCGC
GGACCGCGCGCGCATAATCT

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 411; Mature: 410

Protein sequence:

>411_residues
MSWQLAQSPSCAKLYAAPGNPGIELHAECVPIAADDLDGLIVFVRAHAIDFVVVGPEAPLVAGLADRLRAIGVPVFGPSA
AAARLEGSKGFTKDLCARASIPTAAYVRCTSADEALAALDGFDVPVVIKADGLAAGKGVIIAETRAEAEAAIADMFAGAF
GGAGAEVVIEEFMTGEEASFFALSDGQDVIAFGSAQDHKRVGDGDTGPNTGGMGAYSPAPVLTPDLEVAVMERIIRPTVG
TLAAEATPYVGVLFAGLMLTAEGPKLIEYNCRFGDPECQVLMMRFRGDLAALLHAAATGRLARAEPPTFAHDYALTVVMA
AKGYPGTPEKGGAIRRIADAEVGGVRVFHAGTAREDRTLVAAGGRVLNVTATGKSVTEAQARAYAALDRIDFPTGFCRRD
IGWREVAREAR

Sequences:

>Translated_411_residues
MSWQLAQSPSCAKLYAAPGNPGIELHAECVPIAADDLDGLIVFVRAHAIDFVVVGPEAPLVAGLADRLRAIGVPVFGPSA
AAARLEGSKGFTKDLCARASIPTAAYVRCTSADEALAALDGFDVPVVIKADGLAAGKGVIIAETRAEAEAAIADMFAGAF
GGAGAEVVIEEFMTGEEASFFALSDGQDVIAFGSAQDHKRVGDGDTGPNTGGMGAYSPAPVLTPDLEVAVMERIIRPTVG
TLAAEATPYVGVLFAGLMLTAEGPKLIEYNCRFGDPECQVLMMRFRGDLAALLHAAATGRLARAEPPTFAHDYALTVVMA
AKGYPGTPEKGGAIRRIADAEVGGVRVFHAGTAREDRTLVAAGGRVLNVTATGKSVTEAQARAYAALDRIDFPTGFCRRD
IGWREVAREAR
>Mature_410_residues
SWQLAQSPSCAKLYAAPGNPGIELHAECVPIAADDLDGLIVFVRAHAIDFVVVGPEAPLVAGLADRLRAIGVPVFGPSAA
AARLEGSKGFTKDLCARASIPTAAYVRCTSADEALAALDGFDVPVVIKADGLAAGKGVIIAETRAEAEAAIADMFAGAFG
GAGAEVVIEEFMTGEEASFFALSDGQDVIAFGSAQDHKRVGDGDTGPNTGGMGAYSPAPVLTPDLEVAVMERIIRPTVGT
LAAEATPYVGVLFAGLMLTAEGPKLIEYNCRFGDPECQVLMMRFRGDLAALLHAAATGRLARAEPPTFAHDYALTVVMAA
KGYPGTPEKGGAIRRIADAEVGGVRVFHAGTAREDRTLVAAGGRVLNVTATGKSVTEAQARAYAALDRIDFPTGFCRRDI
GWREVAREAR

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=411, Percent_Identity=45.0121654501217, Blast_Score=343, Evalue=1e-94,
Organism=Homo sapiens, GI209869995, Length=411, Percent_Identity=45.0121654501217, Blast_Score=343, Evalue=1e-94,
Organism=Homo sapiens, GI209869993, Length=411, Percent_Identity=45.0121654501217, Blast_Score=343, Evalue=1e-94,
Organism=Homo sapiens, GI28416899, Length=411, Percent_Identity=45.0121654501217, Blast_Score=343, Evalue=2e-94,
Organism=Escherichia coli, GI1790438, Length=416, Percent_Identity=53.125, Blast_Score=409, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI17567511, Length=409, Percent_Identity=39.119804400978, Blast_Score=253, Evalue=1e-67,
Organism=Saccharomyces cerevisiae, GI6321203, Length=420, Percent_Identity=41.6666666666667, Blast_Score=291, Evalue=1e-79,
Organism=Drosophila melanogaster, GI62471619, Length=401, Percent_Identity=41.645885286783, Blast_Score=266, Evalue=2e-71,
Organism=Drosophila melanogaster, GI24582400, Length=401, Percent_Identity=41.645885286783, Blast_Score=265, Evalue=4e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 42672; Mature: 42541

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSWQLAQSPSCAKLYAAPGNPGIELHAECVPIAADDLDGLIVFVRAHAIDFVVVGPEAPL
CCCCCCCCCCCEEEEECCCCCCCEEEEEEEEEECCCCCCEEEEEEECEEEEEEECCCCCH
VAGLADRLRAIGVPVFGPSAAAARLEGSKGFTKDLCARASIPTAAYVRCTSADEALAALD
HHHHHHHHHHHCCCCCCCCHHHHEECCCCCCHHHHHHHCCCCCEEEEEECCCHHHHHHHC
GFDVPVVIKADGLAAGKGVIIAETRAEAEAAIADMFAGAFGGAGAEVVIEEFMTGEEASF
CCCCEEEEEECCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCEE
FALSDGQDVIAFGSAQDHKRVGDGDTGPNTGGMGAYSPAPVLTPDLEVAVMERIIRPTVG
EEECCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHH
TLAAEATPYVGVLFAGLMLTAEGPKLIEYNCRFGDPECQVLMMRFRGDLAALLHAAATGR
HHHHCCCCHHHHHHHHHEEECCCCEEEEEECCCCCCHHHHHHHHHCCHHHHHHHHHHCCC
LARAEPPTFAHDYALTVVMAAKGYPGTPEKGGAIRRIADAEVGGVRVFHAGTAREDRTLV
CCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCEEEECCCCCCCEEEEECCCCCCCCEEE
AAGGRVLNVTATGKSVTEAQARAYAALDRIDFPTGFCRRDIGWREVAREAR
EECCEEEEEEECCCHHHHHHHHHHHHHHHCCCCCHHHHCCCCHHHHHHHCC
>Mature Secondary Structure 
SWQLAQSPSCAKLYAAPGNPGIELHAECVPIAADDLDGLIVFVRAHAIDFVVVGPEAPL
CCCCCCCCCCEEEEECCCCCCCEEEEEEEEEECCCCCCEEEEEEECEEEEEEECCCCCH
VAGLADRLRAIGVPVFGPSAAAARLEGSKGFTKDLCARASIPTAAYVRCTSADEALAALD
HHHHHHHHHHHCCCCCCCCHHHHEECCCCCCHHHHHHHCCCCCEEEEEECCCHHHHHHHC
GFDVPVVIKADGLAAGKGVIIAETRAEAEAAIADMFAGAFGGAGAEVVIEEFMTGEEASF
CCCCEEEEEECCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCEE
FALSDGQDVIAFGSAQDHKRVGDGDTGPNTGGMGAYSPAPVLTPDLEVAVMERIIRPTVG
EEECCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHH
TLAAEATPYVGVLFAGLMLTAEGPKLIEYNCRFGDPECQVLMMRFRGDLAALLHAAATGR
HHHHCCCCHHHHHHHHHEEECCCCEEEEEECCCCCCHHHHHHHHHCCHHHHHHHHHHCCC
LARAEPPTFAHDYALTVVMAAKGYPGTPEKGGAIRRIADAEVGGVRVFHAGTAREDRTLV
CCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCEEEECCCCCCCEEEEECCCCCCCCEEE
AAGGRVLNVTATGKSVTEAQARAYAALDRIDFPTGFCRRDIGWREVAREAR
EECCEEEEEEECCCHHHHHHHHHHHHHHHCCCCCHHHHCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA