Click here to switch to the map view.

The map label for this gene is bglIIM [H]

Identifier: 103487781

GI number: 103487781

Start: 2427469

End: 2428332

Strand: Direct

Name: bglIIM [H]

Synonym: Sala_2300

Alternate gene names: 103487781

Gene position: 2427469-2428332 (Clockwise)

Preceding gene: 103487780

Following gene: 103487782

Centisome position: 72.57

GC content: 53.12

Gene sequence:

>864_bases
ATGCGCCGCAATACCGTCACCAAGCTGAAATCGGACCGTTCGGTATATATGGCTTGTCAGGACAATCTCGCTTTTATGCG
CCCGCTGCCCGACGAGAGCATGAAGCTCATCGTGACCTCGCCGCCATACAATATAGGCAAAGCATACGAGCAACGCTCTC
CTCTGGCAGAATACGTCAAGGGGCAAGCGCAAGTAATTTCGGAGTGTGTTCGGTTGCTTTCCAAGGGTGGGTCGCTCTGC
TGGCAGGTTGGAAATCACGTTGACCGAGGCGAAATCTTCCCGCTTGATATGGTGCTTTACCCGATTTTCAAAGAGCACGG
CCTCAACCTACGCAATCGGGTGATCTGGCATTTCGAGCATGGCTTGCACTGCTCCAAACGGTTGTCTGGCCGCTATGAGA
CCATTCTCTGGTTCACAAAGGGTGATAGCTATCATTTCGACGTCGACCCCATCCGGGTTCCGGCCAAGTATCCGGGTAAG
AAGCATTTTAAGGGGCCTAAAGCAGGGCAACTCTCCGGCAATCCCCTTGGGAAAAATCCTGGGGATGTGTGGATATTTCC
CAACGTCAAATCCAATCACGTCGAGAAGACGAGTCATCCATGTCAGTTCCCTGTCGAACTGGTCGAGAGGCTAGTATTGG
CATTGACTGAACCCGGTGACGCCGTATTTGATCCTTACATGGGGGTAGGCTCCTCTGTCGTTGCAGCTGCCATGCACGAT
CGCATAGGCTATGGCTGTGACGTGGTATCGGAGTACGTCGACATTGCATGGCACCGGGTTCACGCATTGCGCGCTGGAAC
TTTGAAAACTCGACCAATGAACAAGCCGGTTTATGATCCTAGCTTGCCGAAGGGCGGGCACTAA

Upstream 100 bases:

>100_bases
ATCCAAGGAAATAGATCGAAATCGGTATGTTGCTTGCCACAAGGCTGATGACATGTTCCCCATATATTCTTATACGGTCG
GAACCTTAGGAGAACGACCA

Downstream 100 bases:

>100_bases
TGAGGATCGTTCAATACTATTCTCATCTTAACGGCTTCGAGTTCCTCAAGTATCACAAAGCCCACATCTGGGATGATCTC
GAAGCCGTCGTAGCTGCTGT

Product: DNA methylase N-4/N-6

Products: NA

Alternate protein names: M.BglII; N(4)- cytosine-specific methyltransferase BglII [H]

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MRRNTVTKLKSDRSVYMACQDNLAFMRPLPDESMKLIVTSPPYNIGKAYEQRSPLAEYVKGQAQVISECVRLLSKGGSLC
WQVGNHVDRGEIFPLDMVLYPIFKEHGLNLRNRVIWHFEHGLHCSKRLSGRYETILWFTKGDSYHFDVDPIRVPAKYPGK
KHFKGPKAGQLSGNPLGKNPGDVWIFPNVKSNHVEKTSHPCQFPVELVERLVLALTEPGDAVFDPYMGVGSSVVAAAMHD
RIGYGCDVVSEYVDIAWHRVHALRAGTLKTRPMNKPVYDPSLPKGGH

Sequences:

>Translated_287_residues
MRRNTVTKLKSDRSVYMACQDNLAFMRPLPDESMKLIVTSPPYNIGKAYEQRSPLAEYVKGQAQVISECVRLLSKGGSLC
WQVGNHVDRGEIFPLDMVLYPIFKEHGLNLRNRVIWHFEHGLHCSKRLSGRYETILWFTKGDSYHFDVDPIRVPAKYPGK
KHFKGPKAGQLSGNPLGKNPGDVWIFPNVKSNHVEKTSHPCQFPVELVERLVLALTEPGDAVFDPYMGVGSSVVAAAMHD
RIGYGCDVVSEYVDIAWHRVHALRAGTLKTRPMNKPVYDPSLPKGGH
>Mature_287_residues
MRRNTVTKLKSDRSVYMACQDNLAFMRPLPDESMKLIVTSPPYNIGKAYEQRSPLAEYVKGQAQVISECVRLLSKGGSLC
WQVGNHVDRGEIFPLDMVLYPIFKEHGLNLRNRVIWHFEHGLHCSKRLSGRYETILWFTKGDSYHFDVDPIRVPAKYPGK
KHFKGPKAGQLSGNPLGKNPGDVWIFPNVKSNHVEKTSHPCQFPVELVERLVLALTEPGDAVFDPYMGVGSSVVAAAMHD
RIGYGCDVVSEYVDIAWHRVHALRAGTLKTRPMNKPVYDPSLPKGGH

Specific function: This methylase recognizes the double-stranded sequence AGATCT, causes specific methylation on C-5 on both strands, and protects the DNA from cleavage by the BglII endonuclease [H]

COG id: COG0863

COG function: function code L; DNA modification methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family. N(4) subfamily [H]

Homologues:

Organism=Escherichia coli, GI87082238, Length=249, Percent_Identity=30.9236947791165, Blast_Score=110, Evalue=8e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002941
- InterPro:   IPR017985
- InterPro:   IPR001091 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.113 [H]

Molecular weight: Translated: 32293; Mature: 32293

Theoretical pI: Translated: 9.41; Mature: 9.41

Prosite motif: PS00093 N4_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRNTVTKLKSDRSVYMACQDNLAFMRPLPDESMKLIVTSPPYNIGKAYEQRSPLAEYVK
CCCCHHHHHCCCCEEEEEECCCCEEECCCCCCCCEEEEECCCCCHHHHHHHCCCHHHHHC
GQAQVISECVRLLSKGGSLCWQVGNHVDRGEIFPLDMVLYPIFKEHGLNLRNRVIWHFEH
CHHHHHHHHHHHHHCCCCEEEHHCCCCCCCCCCCHHHHHHHHHHHHCCCCHHEEEEEECC
GLHCSKRLSGRYETILWFTKGDSYHFDVDPIRVPAKYPGKKHFKGPKAGQLSGNPLGKNP
CCCHHHHCCCCEEEEEEEECCCCEEECCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCC
GDVWIFPNVKSNHVEKTSHPCQFPVELVERLVLALTEPGDAVFDPYMGVGSSVVAAAMHD
CCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHCCCCCCCHHHHHHHHHH
RIGYGCDVVSEYVDIAWHRVHALRAGTLKTRPMNKPVYDPSLPKGGH
HCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRRNTVTKLKSDRSVYMACQDNLAFMRPLPDESMKLIVTSPPYNIGKAYEQRSPLAEYVK
CCCCHHHHHCCCCEEEEEECCCCEEECCCCCCCCEEEEECCCCCHHHHHHHCCCHHHHHC
GQAQVISECVRLLSKGGSLCWQVGNHVDRGEIFPLDMVLYPIFKEHGLNLRNRVIWHFEH
CHHHHHHHHHHHHHCCCCEEEHHCCCCCCCCCCCHHHHHHHHHHHHCCCCHHEEEEEECC
GLHCSKRLSGRYETILWFTKGDSYHFDVDPIRVPAKYPGKKHFKGPKAGQLSGNPLGKNP
CCCHHHHCCCCEEEEEEEECCCCEEECCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCC
GDVWIFPNVKSNHVEKTSHPCQFPVELVERLVLALTEPGDAVFDPYMGVGSSVVAAAMHD
CCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHCCCCCCCHHHHHHHHHH
RIGYGCDVVSEYVDIAWHRVHALRAGTLKTRPMNKPVYDPSLPKGGH
HCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9073062 [H]