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The map label for this gene is cca [H]

Identifier: 103487591

GI number: 103487591

Start: 2218445

End: 2219641

Strand: Reverse

Name: cca [H]

Synonym: Sala_2110

Alternate gene names: 103487591

Gene position: 2219641-2218445 (Counterclockwise)

Preceding gene: 103487592

Following gene: 103487587

Centisome position: 66.35

GC content: 71.93

Gene sequence:

>1197_bases
GTGACGCGGCTGCCCGACGCCGCCTGGCGCGACCGGCCGGGGCTGCGCCGCATCGTCGCCGCGCTTGCCGACGATGGCGG
CGCGGTCAAGGTCGTCGGTGGCGCGGTGCGCGACACCTTGCTCGGCCTTGATGTCACCGACGTCGACCTTGCGACCCCGC
TTGTCCCCGGAGAGGTGACGCGGCGGCTGGAAGCGGCGGGAATCAAGGTGATCCCGACCGGAATCGCGCACGGCACCGTC
ACCGCGATCGCCAGCGGCGACCATCACGAGATCACGACGCTTCGCCGCGACGTCGCGACCGACGGCCGCCGTGCGACAGT
GGCCTTCGCCGACGACTGGCGCGAGGATGCGGCGCGCCGCGACTTCACGATCAACGCGCTTTATGCCGATCCCCGGACGG
GCGCGATCGACGACTGGTTCGGGGGCCTTGCGGATCTTGACCGCGGCCGCGTCGCGTTCATCGGCGACGCCGCCTCGCGC
ATTGCCGAGGATCATCTGCGCATCCTGCGCTTTTACCGCTTCGCCGCGCGCTTCGGGCGTGGCGACCTCGATCCCGCAAG
CCATGCCGCGGTTGTCGCGGCGCGGCAATCGCTCAAAAGCCTGTCGCGCGAACGCATCGCCGACGAACTCGCCAAGATAT
TGGCGCTGCCCGACCCGCGCGCGATCGTCGCGCAGATGCACGCCGACGGCATCCTCGCCGTGCTGCTGCCCGAACTCGAC
GCCGGCTTTGCGGCGGCGCTCGACCGGCTGTTCGCAAACGAAGCGGTGGCCGGTGCTGTGCCCGCGGCGCTTCGCCGCCT
CGCCGCGCTGCTCCCCGCCGATGTCGCGATCGCCGAACAGGTGGCGAGCCGCCTGCGCCTGTCGACACGCCAGCGCAAGC
ATCTCGCCGCGCTCGGCGGCCACCGCCACGATGTCGCGCGTCCGGTGCGCCAGCTGGCGTACGCCATCGGGATCGAGGCG
GCGCGCGACGTGCATCTGCTGGCGGGCGATCCTGCCGCGCTTCGGACGCTGTCGGACTGGCAGGTCCCCATGCTCCCGGT
GAAGGGCGGGGACATCGTCGCGCGCGGCATTGCGGCGGGGCCTGAGGTGGCGCGCATCCTGAAGGCGGTCGAGGCCGCGT
GGGTGGCCGAAGATTTCCCGGACGCCGCGCGCGTCGCCGAGCTGGTCGATCAGAAGATCGGCCGCGTCAGCGACTGA

Upstream 100 bases:

>100_bases
GGCATTATTATGACATGGAGTGGCAGGGACGGCGGATCTGGGGCGTGACCGCGGGTATCATCGTCAATCTGGCGCGCCGC
CTGCCCGCGGGGTGGCACCG

Downstream 100 bases:

>100_bases
CGCCAGTAATCGAGCGCCGCCTTGCCATCCATCGGCCGCGCGAACAGATAGCCCTGCCCGAAGTCGCAGCCGAGCGCCGA
CAGCAGCCGCGCCTGATCGG

Product: polynucleotide adenylyltransferase region

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 398; Mature: 397

Protein sequence:

>398_residues
MTRLPDAAWRDRPGLRRIVAALADDGGAVKVVGGAVRDTLLGLDVTDVDLATPLVPGEVTRRLEAAGIKVIPTGIAHGTV
TAIASGDHHEITTLRRDVATDGRRATVAFADDWREDAARRDFTINALYADPRTGAIDDWFGGLADLDRGRVAFIGDAASR
IAEDHLRILRFYRFAARFGRGDLDPASHAAVVAARQSLKSLSRERIADELAKILALPDPRAIVAQMHADGILAVLLPELD
AGFAAALDRLFANEAVAGAVPAALRRLAALLPADVAIAEQVASRLRLSTRQRKHLAALGGHRHDVARPVRQLAYAIGIEA
ARDVHLLAGDPAALRTLSDWQVPMLPVKGGDIVARGIAAGPEVARILKAVEAAWVAEDFPDAARVAELVDQKIGRVSD

Sequences:

>Translated_398_residues
MTRLPDAAWRDRPGLRRIVAALADDGGAVKVVGGAVRDTLLGLDVTDVDLATPLVPGEVTRRLEAAGIKVIPTGIAHGTV
TAIASGDHHEITTLRRDVATDGRRATVAFADDWREDAARRDFTINALYADPRTGAIDDWFGGLADLDRGRVAFIGDAASR
IAEDHLRILRFYRFAARFGRGDLDPASHAAVVAARQSLKSLSRERIADELAKILALPDPRAIVAQMHADGILAVLLPELD
AGFAAALDRLFANEAVAGAVPAALRRLAALLPADVAIAEQVASRLRLSTRQRKHLAALGGHRHDVARPVRQLAYAIGIEA
ARDVHLLAGDPAALRTLSDWQVPMLPVKGGDIVARGIAAGPEVARILKAVEAAWVAEDFPDAARVAELVDQKIGRVSD
>Mature_397_residues
TRLPDAAWRDRPGLRRIVAALADDGGAVKVVGGAVRDTLLGLDVTDVDLATPLVPGEVTRRLEAAGIKVIPTGIAHGTVT
AIASGDHHEITTLRRDVATDGRRATVAFADDWREDAARRDFTINALYADPRTGAIDDWFGGLADLDRGRVAFIGDAASRI
AEDHLRILRFYRFAARFGRGDLDPASHAAVVAARQSLKSLSRERIADELAKILALPDPRAIVAQMHADGILAVLLPELDA
GFAAALDRLFANEAVAGAVPAALRRLAALLPADVAIAEQVASRLRLSTRQRKHLAALGGHRHDVARPVRQLAYAIGIEAA
RDVHLLAGDPAALRTLSDWQVPMLPVKGGDIVARGIAAGPEVARILKAVEAAWVAEDFPDAARVAELVDQKIGRVSD

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=385, Percent_Identity=31.6883116883117, Blast_Score=184, Evalue=1e-46,
Organism=Escherichia coli, GI87081691, Length=176, Percent_Identity=32.9545454545455, Blast_Score=82, Evalue=9e-17,
Organism=Escherichia coli, GI1789436, Length=315, Percent_Identity=28.2539682539683, Blast_Score=77, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI71995920, Length=406, Percent_Identity=31.2807881773399, Blast_Score=166, Evalue=1e-41,
Organism=Drosophila melanogaster, GI281360112, Length=380, Percent_Identity=31.8421052631579, Blast_Score=166, Evalue=2e-41,
Organism=Drosophila melanogaster, GI21357337, Length=380, Percent_Identity=31.8421052631579, Blast_Score=166, Evalue=2e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 42399; Mature: 42268

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRLPDAAWRDRPGLRRIVAALADDGGAVKVVGGAVRDTLLGLDVTDVDLATPLVPGEVT
CCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECHHHHHHHHCCCCCCCHHCCCCCCHHHH
RRLEAAGIKVIPTGIAHGTVTAIASGDHHEITTLRRDVATDGRRATVAFADDWREDAARR
HHHHHCCEEEEECCCCCCCEEEEECCCCHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHC
DFTINALYADPRTGAIDDWFGGLADLDRGRVAFIGDAASRIAEDHLRILRFYRFAARFGR
CEEEEEEEECCCCCCCHHHHCCHHHCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHCC
GDLDPASHAAVVAARQSLKSLSRERIADELAKILALPDPRAIVAQMHADGILAVLLPELD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCEEEEEECCCC
AGFAAALDRLFANEAVAGAVPAALRRLAALLPADVAIAEQVASRLRLSTRQRKHLAALGG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
HRHDVARPVRQLAYAIGIEAARDVHLLAGDPAALRTLSDWQVPMLPVKGGDIVARGIAAG
CHHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHCCCCCEEEEECCCCEEECCCCCC
PEVARILKAVEAAWVAEDFPDAARVAELVDQKIGRVSD
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TRLPDAAWRDRPGLRRIVAALADDGGAVKVVGGAVRDTLLGLDVTDVDLATPLVPGEVT
CCCCCCCCCCCCHHHHHHHHHHCCCCEEEEECHHHHHHHHCCCCCCCHHCCCCCCHHHH
RRLEAAGIKVIPTGIAHGTVTAIASGDHHEITTLRRDVATDGRRATVAFADDWREDAARR
HHHHHCCEEEEECCCCCCCEEEEECCCCHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHC
DFTINALYADPRTGAIDDWFGGLADLDRGRVAFIGDAASRIAEDHLRILRFYRFAARFGR
CEEEEEEEECCCCCCCHHHHCCHHHCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHCC
GDLDPASHAAVVAARQSLKSLSRERIADELAKILALPDPRAIVAQMHADGILAVLLPELD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCEEEEEECCCC
AGFAAALDRLFANEAVAGAVPAALRRLAALLPADVAIAEQVASRLRLSTRQRKHLAALGG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
HRHDVARPVRQLAYAIGIEAARDVHLLAGDPAALRTLSDWQVPMLPVKGGDIVARGIAAG
CHHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHCCCCCEEEEECCCCEEECCCCCC
PEVARILKAVEAAWVAEDFPDAARVAELVDQKIGRVSD
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA