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The map label for this gene is mhpC [H]

Identifier: 103487429

GI number: 103487429

Start: 2049670

End: 2050710

Strand: Reverse

Name: mhpC [H]

Synonym: Sala_1945

Alternate gene names: 103487429

Gene position: 2050710-2049670 (Counterclockwise)

Preceding gene: 103487430

Following gene: 103487422

Centisome position: 61.3

GC content: 66.57

Gene sequence:

>1041_bases
ATGATAGCCGACGACGATCTGGATTTCGATCCGCCGCCTCCGCCGCGGCGGCCCTTTTTCCGGCGCAAGCGCGTCCTGAT
CCCGCTTGCCGTGCTGGCGGCGCTCGTTATCGGCTTTCTGCTGCTCCTGACCCCCGATACCGACCGCGAGGCGATGATCG
CCAAATATGGCGGACCGGACGCCGCCTTTGCCGCAGGGCCGGCGGGGCAGCGCATTCACTATCGCGATCAGGGACGCCGC
GACGGCCCGGCGATCATGCTGCTCCATGGATCGAACGCCAGCCTTCATACATGGGAGCCGCTGGTCGAACGGCTCGGCGC
CGATTACCGCATCGTCACGCTCGATCTGCCGGGGCACGGGCTGACCGGGGCGATTCCCGGTCGCGACTATGACGCCGATG
CGATGATGGAAGCGGTCGATGTCGTCGCGGCCAAGCTGGGGCTCCACCATTTCGTTCTCGGCGGCAATTCGATGGGCGGC
TGGATCGCCTGGCGCTATGCGCTCGCGCATCCCGCGCGCGTCGATGCGCTGCTCCTCATCGACGCCGCGGGGATGCCGCT
GCGCAGGGGGGAGAAGGCCCCCGAATCGAACGTCGGCTTTCGCGTGCTCGAATATCCGTTCGGACGCTGGCTCGCGACGC
GGATCACGCCGCGGATGCTCGTCGAACAGTCGCTGTTGGGGTCGGTCGAAAGGCAGGATATCGTCGATGCAGCGATGATC
GACCGCTATTGGGAACTGCTGCGCTTCCCCGGCAACCGCGAGGCGACGGTGATCCGCGCGCGCGCCAGCCGCGAACCCGA
AATGGCGGCGCGGGTGGGGGAGATTACGGCGCCGACGCTGATCCTGTTCGGGGACAAGGACCGGATTATCAACCCGAGCG
CGGCAAAGACGTTCAACGAGCGGATCGCGGGGTCGGAGGTCGTATTGCTCCCTGGAATCGGCCATTTGCCGATGGAAGAA
GCGCCCGATCAGGTTGCCACGGCGATTGCCGATTTCCTCACGCGGCGGCTTGCAGCGGCGGCCGCTCCAGAAAGGCGCTG
A

Upstream 100 bases:

>100_bases
GTTCCTGCCCGAAACCTTCCGCCGGTCGATCCATCAATAGGCGTGCATTGACGCCCGGCGCCCGCACGCTAGGTTTGCGC
GGCCTTGAGGGGGGCGTACG

Downstream 100 bases:

>100_bases
TCCGCGCCGCGACCGTCCCGGCGTGGGTGAAGGGAAAAAGGTGCGAGCCCGGCACCACCTCCAGCACCGCGCCGTCGATC
AGCTCGGCGATCGTCTGTCC

Product: alpha/beta hydrolase fold

Products: 2-oxopent-4-enoate; succinate [C]

Alternate protein names: 2-hydroxy-6-ketonona-2,4-diene-1,9-dioic acid 5,6-hydrolase; 2-hydroxy-6-oxonona-2,4,7-triene-1,9-dioic acid 5,6-hydrolase; 2-hydroxy-6-oxonona-2,4-diene-1,9-dioic acid 5,6-hydrolase [H]

Number of amino acids: Translated: 346; Mature: 346

Protein sequence:

>346_residues
MIADDDLDFDPPPPPRRPFFRRKRVLIPLAVLAALVIGFLLLLTPDTDREAMIAKYGGPDAAFAAGPAGQRIHYRDQGRR
DGPAIMLLHGSNASLHTWEPLVERLGADYRIVTLDLPGHGLTGAIPGRDYDADAMMEAVDVVAAKLGLHHFVLGGNSMGG
WIAWRYALAHPARVDALLLIDAAGMPLRRGEKAPESNVGFRVLEYPFGRWLATRITPRMLVEQSLLGSVERQDIVDAAMI
DRYWELLRFPGNREATVIRARASREPEMAARVGEITAPTLILFGDKDRIINPSAAKTFNERIAGSEVVLLPGIGHLPMEE
APDQVATAIADFLTRRLAAAAAPERR

Sequences:

>Translated_346_residues
MIADDDLDFDPPPPPRRPFFRRKRVLIPLAVLAALVIGFLLLLTPDTDREAMIAKYGGPDAAFAAGPAGQRIHYRDQGRR
DGPAIMLLHGSNASLHTWEPLVERLGADYRIVTLDLPGHGLTGAIPGRDYDADAMMEAVDVVAAKLGLHHFVLGGNSMGG
WIAWRYALAHPARVDALLLIDAAGMPLRRGEKAPESNVGFRVLEYPFGRWLATRITPRMLVEQSLLGSVERQDIVDAAMI
DRYWELLRFPGNREATVIRARASREPEMAARVGEITAPTLILFGDKDRIINPSAAKTFNERIAGSEVVLLPGIGHLPMEE
APDQVATAIADFLTRRLAAAAAPERR
>Mature_346_residues
MIADDDLDFDPPPPPRRPFFRRKRVLIPLAVLAALVIGFLLLLTPDTDREAMIAKYGGPDAAFAAGPAGQRIHYRDQGRR
DGPAIMLLHGSNASLHTWEPLVERLGADYRIVTLDLPGHGLTGAIPGRDYDADAMMEAVDVVAAKLGLHHFVLGGNSMGG
WIAWRYALAHPARVDALLLIDAAGMPLRRGEKAPESNVGFRVLEYPFGRWLATRITPRMLVEQSLLGSVERQDIVDAAMI
DRYWELLRFPGNREATVIRARASREPEMAARVGEITAPTLILFGDKDRIINPSAAKTFNERIAGSEVVLLPGIGHLPMEE
APDQVATAIADFLTRRLAAAAAPERR

Specific function: Catalyzes the cleavage of the C5-C6 bond of 2-hydroxy-6- oxononadienedioate and 2-hydroxy-6-oxononatrienedioate, a dienol ring fission product of the bacterial meta-cleavage pathway for degradation of phenylpropionic acid [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. MhpC family [H]

Homologues:

Organism=Homo sapiens, GI218777837, Length=277, Percent_Identity=24.5487364620939, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI87081721, Length=276, Percent_Identity=29.3478260869565, Blast_Score=85, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR000639 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.7.1.- [C]

Molecular weight: Translated: 37919; Mature: 37919

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIADDDLDFDPPPPPRRPFFRRKRVLIPLAVLAALVIGFLLLLTPDTDREAMIAKYGGPD
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCC
AAFAAGPAGQRIHYRDQGRRDGPAIMLLHGSNASLHTWEPLVERLGADYRIVTLDLPGHG
CCCCCCCCCCEEEECCCCCCCCCEEEEEECCCCCCCHHHHHHHHCCCCEEEEEEECCCCC
LTGAIPGRDYDADAMMEAVDVVAAKLGLHHFVLGGNSMGGWIAWRYALAHPARVDALLLI
CCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHCCCCCCCEEEEE
DAAGMPLRRGEKAPESNVGFRVLEYPFGRWLATRITPRMLVEQSLLGSVERQDIVDAAMI
ECCCCCHHCCCCCCCCCCCEEEEECCHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHH
DRYWELLRFPGNREATVIRARASREPEMAARVGEITAPTLILFGDKDRIINPSAAKTFNE
HHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHH
RIAGSEVVLLPGIGHLPMEEAPDQVATAIADFLTRRLAAAAAPERR
HHCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MIADDDLDFDPPPPPRRPFFRRKRVLIPLAVLAALVIGFLLLLTPDTDREAMIAKYGGPD
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCC
AAFAAGPAGQRIHYRDQGRRDGPAIMLLHGSNASLHTWEPLVERLGADYRIVTLDLPGHG
CCCCCCCCCCEEEECCCCCCCCCEEEEEECCCCCCCHHHHHHHHCCCCEEEEEEECCCCC
LTGAIPGRDYDADAMMEAVDVVAAKLGLHHFVLGGNSMGGWIAWRYALAHPARVDALLLI
CCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHCCCCCCCEEEEE
DAAGMPLRRGEKAPESNVGFRVLEYPFGRWLATRITPRMLVEQSLLGSVERQDIVDAAMI
ECCCCCHHCCCCCCCCCCCEEEEECCHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHH
DRYWELLRFPGNREATVIRARASREPEMAARVGEITAPTLILFGDKDRIINPSAAKTFNE
HHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHH
RIAGSEVVLLPGIGHLPMEEAPDQVATAIADFLTRRLAAAAAPERR
HHCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-hydroxy-6-ketononadienedicarboxylate; H2O [C]

Specific reaction: 2-hydroxy-6-ketononadienedicarboxylate + H2O = 2-oxopent-4-enoate + succinate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA