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The map label for this gene is proP [H]

Identifier: 103487430

GI number: 103487430

Start: 2050771

End: 2052396

Strand: Reverse

Name: proP [H]

Synonym: Sala_1946

Alternate gene names: 103487430

Gene position: 2052396-2050771 (Counterclockwise)

Preceding gene: 103487431

Following gene: 103487429

Centisome position: 61.35

GC content: 63.1

Gene sequence:

>1626_bases
ATGATCGACGCAGAGGCAGCGGCACCTATCGTCACCCGCCAGAGCGAACGCAAGGTCATCCTCGCCTCGTCGCTCGGTAC
GGTGTTCGAATGGTATGATTTCTACCTCTACGGCCTGCTCGCGACGGTCATTTCGGCGCAGTTCTTCTCTGGCGTCAACG
AAACCACGGGCTTCATCTTTGCGCTTGCCGCCTTTGCCGCGGGCTTTGCGGTGCGGCCCTTTGGCGCAGTTGTCTTCGGG
CGCATAGGCGACCTCGTCGGGCGCAAGAATACGTTTCTGGTGACCATGGGGCTGATGGGCGCCTCGACGTTCCTCGTCGG
CCTGCTGCCCAGCTATGCGTCGATCGGGGTCGCGGCGCCGATCCTGCTCGTTGTCTTGCGCCTTGTGCAAGGACTCGCGC
TTGGCGGCGAATATGGCGGCGCTGCCACTTATGTTGCCGAACATGCGCCCGAGGGGAAGCGCGGGCTGTTCACCAGCTTC
ATCCAGACCACCGCGACGCTCGGCCTGTTCGCGGCGCTCGGCGTCGTCATCGTCATCCGCTCGGCGATGGGCGAGGCGGC
GTTTGCCGACTGGGGCTGGCGCATCCCCTTCCTCATTTCAATCGTCCTGCTCGGCATTTCGCTCTGGATCCGCCTCCAGC
TCGAGGAAAGCCCGGTCTTCAGGCAGATGAAGGCAGAGGGGACGACGTCGAAGGCGCCGTTGACCGAAGCCTTCGGCCGC
TGGGTAAATCTCAAATGGGTGCTCGTTGCGCTGTTCGGCGCCGTCGCGGGGCAGGCGGTCGTCTGGTACACGGGGCAATT
CTATGCGCTATTCTTCCTCGAAAAGACGCTGAAGGTCGATGGCGCGGCGGCGAACATATTGATCGCCATCGCGCTTGCAC
TCGGCACACCCTTTTTCATCCTCTTCGGCTGGCTCAGCGACAAGGTGGGGCGCAAACCGATCATCCTCACGGGCTGTGCC
CTCGCGGCGCTGACCTATTTCCCGGTGTTCCAGATGCTGACCGCTGCGGCCAATCCGGCGCTCGCCGAGGCACAGGCGCG
GGCGCCTGTTGTCGTGTTCGCCCATCCCGAGACATGCTCGTTCCAGTTTGACCCTATTGGCCGCACCTCGTTCGACCGGA
CGGGCTGCGACATCGCCAAGTCAGCGCTCGCCAGAGCGGGGATCCCTTATGCCAATGAGGATGGACCATATGATGGTCGC
GCGCTTGTCCGCGTCGGCGATGAAGACATCCCGGTGATCGACAAGGCCCAATTCGGCGCTGCGCTTACACCCGCGCTCGC
CGCGGCCGGCTATCCACCGAAAGCCGATCCCGCCGCGATCGACAAGCCGCTCGTCGTCGCGATCCTCTTCTACCTCGTGC
TGCTCGTGACGATGGTTTATGGCCCGATCGCCGCTTTGCTCGTGGAGCTGTTTCCCAGCCGCATCCGTTACACCGCCATG
TCGTTGCCCTATCATATCGGCAACGGCTGGTTCGGCGGATTTCTGCCGACGACGGCGTTCGCGATGGTCGCCGCGACGGG
CAATATCTATTACGGCCTCTGGTATCCGGTCATCGTCGCGGTGATCACGCTGGTGGTCGGCCTGTTGTTCCTGCCCGAAA
CCTTCCGCCGGTCGATCCATCAATAG

Upstream 100 bases:

>100_bases
ACAAAGGCGCGGTGAAGCGCGTCGGTTGACGCACTGTTGACATGCGTTAGACCCTCTTGGCCCCTCTCCTTCAGGGGAGG
GGCTTGGCAAGGGGCAGCGG

Downstream 100 bases:

>100_bases
GCGTGCATTGACGCCCGGCGCCCGCACGCTAGGTTTGCGCGGCCTTGAGGGGGGCGTACGATGATAGCCGACGACGATCT
GGATTTCGATCCGCCGCCTC

Product: major facilitator transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 541; Mature: 541

Protein sequence:

>541_residues
MIDAEAAAPIVTRQSERKVILASSLGTVFEWYDFYLYGLLATVISAQFFSGVNETTGFIFALAAFAAGFAVRPFGAVVFG
RIGDLVGRKNTFLVTMGLMGASTFLVGLLPSYASIGVAAPILLVVLRLVQGLALGGEYGGAATYVAEHAPEGKRGLFTSF
IQTTATLGLFAALGVVIVIRSAMGEAAFADWGWRIPFLISIVLLGISLWIRLQLEESPVFRQMKAEGTTSKAPLTEAFGR
WVNLKWVLVALFGAVAGQAVVWYTGQFYALFFLEKTLKVDGAAANILIAIALALGTPFFILFGWLSDKVGRKPIILTGCA
LAALTYFPVFQMLTAAANPALAEAQARAPVVVFAHPETCSFQFDPIGRTSFDRTGCDIAKSALARAGIPYANEDGPYDGR
ALVRVGDEDIPVIDKAQFGAALTPALAAAGYPPKADPAAIDKPLVVAILFYLVLLVTMVYGPIAALLVELFPSRIRYTAM
SLPYHIGNGWFGGFLPTTAFAMVAATGNIYYGLWYPVIVAVITLVVGLLFLPETFRRSIHQ

Sequences:

>Translated_541_residues
MIDAEAAAPIVTRQSERKVILASSLGTVFEWYDFYLYGLLATVISAQFFSGVNETTGFIFALAAFAAGFAVRPFGAVVFG
RIGDLVGRKNTFLVTMGLMGASTFLVGLLPSYASIGVAAPILLVVLRLVQGLALGGEYGGAATYVAEHAPEGKRGLFTSF
IQTTATLGLFAALGVVIVIRSAMGEAAFADWGWRIPFLISIVLLGISLWIRLQLEESPVFRQMKAEGTTSKAPLTEAFGR
WVNLKWVLVALFGAVAGQAVVWYTGQFYALFFLEKTLKVDGAAANILIAIALALGTPFFILFGWLSDKVGRKPIILTGCA
LAALTYFPVFQMLTAAANPALAEAQARAPVVVFAHPETCSFQFDPIGRTSFDRTGCDIAKSALARAGIPYANEDGPYDGR
ALVRVGDEDIPVIDKAQFGAALTPALAAAGYPPKADPAAIDKPLVVAILFYLVLLVTMVYGPIAALLVELFPSRIRYTAM
SLPYHIGNGWFGGFLPTTAFAMVAATGNIYYGLWYPVIVAVITLVVGLLFLPETFRRSIHQ
>Mature_541_residues
MIDAEAAAPIVTRQSERKVILASSLGTVFEWYDFYLYGLLATVISAQFFSGVNETTGFIFALAAFAAGFAVRPFGAVVFG
RIGDLVGRKNTFLVTMGLMGASTFLVGLLPSYASIGVAAPILLVVLRLVQGLALGGEYGGAATYVAEHAPEGKRGLFTSF
IQTTATLGLFAALGVVIVIRSAMGEAAFADWGWRIPFLISIVLLGISLWIRLQLEESPVFRQMKAEGTTSKAPLTEAFGR
WVNLKWVLVALFGAVAGQAVVWYTGQFYALFFLEKTLKVDGAAANILIAIALALGTPFFILFGWLSDKVGRKPIILTGCA
LAALTYFPVFQMLTAAANPALAEAQARAPVVVFAHPETCSFQFDPIGRTSFDRTGCDIAKSALARAGIPYANEDGPYDGR
ALVRVGDEDIPVIDKAQFGAALTPALAAAGYPPKADPAAIDKPLVVAILFYLVLLVTMVYGPIAALLVELFPSRIRYTAM
SLPYHIGNGWFGGFLPTTAFAMVAATGNIYYGLWYPVIVAVITLVVGLLFLPETFRRSIHQ

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=351, Percent_Identity=35.6125356125356, Blast_Score=209, Evalue=3e-55,
Organism=Escherichia coli, GI1788292, Length=327, Percent_Identity=37.6146788990826, Blast_Score=199, Evalue=3e-52,
Organism=Escherichia coli, GI1788942, Length=336, Percent_Identity=35.1190476190476, Blast_Score=172, Evalue=4e-44,
Organism=Escherichia coli, GI1789941, Length=345, Percent_Identity=35.0724637681159, Blast_Score=158, Evalue=1e-39,
Organism=Escherichia coli, GI87082231, Length=222, Percent_Identity=28.3783783783784, Blast_Score=82, Evalue=9e-17,
Organism=Escherichia coli, GI87082404, Length=317, Percent_Identity=24.6056782334385, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 57890; Mature: 57890

Theoretical pI: Translated: 8.37; Mature: 8.37

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDAEAAAPIVTRQSERKVILASSLGTVFEWYDFYLYGLLATVISAQFFSGVNETTGFIF
CCCCHHCCCHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
ALAAFAAGFAVRPFGAVVFGRIGDLVGRKNTFLVTMGLMGASTFLVGLLPSYASIGVAAP
HHHHHHCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHH
ILLVVLRLVQGLALGGEYGGAATYVAEHAPEGKRGLFTSFIQTTATLGLFAALGVVIVIR
HHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
SAMGEAAFADWGWRIPFLISIVLLGISLWIRLQLEESPVFRQMKAEGTTSKAPLTEAFGR
HHHCCHHHHCCCCCHHHHHHHHHHHHHHHEEEEECCCHHHHHHHCCCCCCCCCHHHHHHH
WVNLKWVLVALFGAVAGQAVVWYTGQFYALFFLEKTLKVDGAAANILIAIALALGTPFFI
HCCHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHH
LFGWLSDKVGRKPIILTGCALAALTYFPVFQMLTAAANPALAEAQARAPVVVFAHPETCS
HHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCEEEEECCCCCC
FQFDPIGRTSFDRTGCDIAKSALARAGIPYANEDGPYDGRALVRVGDEDIPVIDKAQFGA
EEECCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCCHHCCC
ALTPALAAAGYPPKADPAAIDKPLVVAILFYLVLLVTMVYGPIAALLVELFPSRIRYTAM
HHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLPYHIGNGWFGGFLPTTAFAMVAATGNIYYGLWYPVIVAVITLVVGLLFLPETFRRSIH
CCCEECCCCCCCHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHCCHHHHHHHC
Q
C
>Mature Secondary Structure
MIDAEAAAPIVTRQSERKVILASSLGTVFEWYDFYLYGLLATVISAQFFSGVNETTGFIF
CCCCHHCCCHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
ALAAFAAGFAVRPFGAVVFGRIGDLVGRKNTFLVTMGLMGASTFLVGLLPSYASIGVAAP
HHHHHHCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHH
ILLVVLRLVQGLALGGEYGGAATYVAEHAPEGKRGLFTSFIQTTATLGLFAALGVVIVIR
HHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
SAMGEAAFADWGWRIPFLISIVLLGISLWIRLQLEESPVFRQMKAEGTTSKAPLTEAFGR
HHHCCHHHHCCCCCHHHHHHHHHHHHHHHEEEEECCCHHHHHHHCCCCCCCCCHHHHHHH
WVNLKWVLVALFGAVAGQAVVWYTGQFYALFFLEKTLKVDGAAANILIAIALALGTPFFI
HCCHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHH
LFGWLSDKVGRKPIILTGCALAALTYFPVFQMLTAAANPALAEAQARAPVVVFAHPETCS
HHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCEEEEECCCCCC
FQFDPIGRTSFDRTGCDIAKSALARAGIPYANEDGPYDGRALVRVGDEDIPVIDKAQFGA
EEECCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCCHHCCC
ALTPALAAAGYPPKADPAAIDKPLVVAILFYLVLLVTMVYGPIAALLVELFPSRIRYTAM
HHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLPYHIGNGWFGGFLPTTAFAMVAATGNIYYGLWYPVIVAVITLVVGLLFLPETFRRSIH
CCCEECCCCCCCHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHCCHHHHHHHC
Q
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]