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The map label for this gene is lcfB [H]

Identifier: 103487414

GI number: 103487414

Start: 2035614

End: 2037146

Strand: Reverse

Name: lcfB [H]

Synonym: Sala_1930

Alternate gene names: 103487414

Gene position: 2037146-2035614 (Counterclockwise)

Preceding gene: 103487419

Following gene: 103487413

Centisome position: 60.9

GC content: 69.02

Gene sequence:

>1533_bases
ATGACCACGCCCGAAAACCCCGTGTCGCAGCCCATCGACCATCTCGCCCGCCGCGGCGCGCCGGATGCGGCTGCGCTGCT
GATCGGCGAACGGGTGGTGACCTATGCCGAACTCGACGCGGGGGTGGGACTGCTTGCGGCGTGGCTACGCGAACAGGCCG
GCGGTCCCGGCGAGCGCGTCGCGAGCTGGAGTGCGAAGACCCGGCTCGCCTGTCTGATGCCGCTCGCCGCGGCGCGCGCG
GGGCTGATCCATGTGCCGATCAATCCGCTGCTCAAGGGGCCGCAGGCGCAGCATATCCTGGCCGACAGCGGCGCGAAGCT
GCTTCTCGCCAACGCCGCGCGCGCCGAGTCGCTGGGCGACGGACGGCCCGCCGGTTGCGCGCTGCACGATCTCAACATCG
CCGAGGAGGCTGTTGATTCGGGAGGCAAGACGTTGCCGCCGTCGAAGGCCGATCCCGACGATCTCGCCGCCATTCTCTAC
ACCAGCGGATCGACCGGGCGGCCCAAGGGGGTGATGCTCAGCCACGCGAACCTGTGGCTCGGCGCCGAAAGCGTCGCGAC
CTACCTGAAGCTCATGCCCGTCGACCGCGTGCTCGGCGTATTGCCCTTGAGCTTCGACTATGGGCAGAATCAGCTGCTGT
CGAGCTGGTATGCCGGCGCCGCGGTGGCGCCGCTCGATTATCTGACCGCGCGCGACGTCGTGAAGGCGGTCGCGCGCCAC
GACATCACGACGCTCGCCGGCGTGCCGCCCCTGTGGGTGCAACTCGTCGAGGCGGACTGGCCCGCCGACACCGCGGCCTT
GCTCAAGCGCCTCACCAACAGTGGTGGCGCGCTCACCCCTTCGCTGATCGAGGCGATGCGCCGCACTTTCCCCGCCGCCG
ACATCTATCCGATGTACGGGCTGACCGAGGCGTTTCGTTCGACCTGGCTCGATCCCAAATTCGTCGCCGACCATCCGACG
TCGATGGGGCGTGCGATCCCGCACGCCGAAATCCTTGTCTGCCGCGGCGACGGCACGATCACCGCCGACGAGGAGCCGGG
CGAGCTGGTGCATTGTGGCCCGCTCGTCGCCAAGGGCTATTGGCGCGACGCGGCGCGCACCGCCGAGCGATTCCGCCCCG
CGCCGCCCGCGTCGCTTTATGGCGGCACCGCGGTCTGGTCGGGCGACAGGGTGCGGCGTGATGCGAACGGTCTTCTCTAT
TTCGTCGGCCGCGACGATGCGATGATCAAGACGGCGGGCAACCGTGTCAGCCCGACCGAGATCGAGGAGGCTGCGGTCGC
TTCGGGGCTGGTCTATGAAGCGGTGGCGTTCGGGGTCCCCGATGCGCGGCTCGGCGCGGCGATCATATTGATCGTGCGCG
GCACGCATGGACCGGCCGACGAGGAGGGGCTTGCTGCCTGGCTTCGCCGGAATCTGCCGAACTTCATGCAGCCGCAGGTC
ATCGAATGGCGCGATGCGCTGCCGCGCAATCCCAATGGCAAGCTCGACCGGGTGGCGATCGCCGCCGATTGGACGAGGGT
GATGGCGGCATGA

Upstream 100 bases:

>100_bases
GGCGAGGCAAACTCCGCGGCGCGCACCGCATCGTCATTATCCCGATCTTCACCGTTCCCTTGCATGGTGCGGCCCTCTAT
CATCTCACTCCTAACAGCCG

Downstream 100 bases:

>100_bases
AGCCGCACGGGCCAATCCCGCCCGGATTCCGGGCCGACCCTGACGGGATGCTGCTGATCGGCGGCGACCGTGCCGAATCG
CTGGCCGATGTCGCGGGCGA

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 510; Mature: 509

Protein sequence:

>510_residues
MTTPENPVSQPIDHLARRGAPDAAALLIGERVVTYAELDAGVGLLAAWLREQAGGPGERVASWSAKTRLACLMPLAAARA
GLIHVPINPLLKGPQAQHILADSGAKLLLANAARAESLGDGRPAGCALHDLNIAEEAVDSGGKTLPPSKADPDDLAAILY
TSGSTGRPKGVMLSHANLWLGAESVATYLKLMPVDRVLGVLPLSFDYGQNQLLSSWYAGAAVAPLDYLTARDVVKAVARH
DITTLAGVPPLWVQLVEADWPADTAALLKRLTNSGGALTPSLIEAMRRTFPAADIYPMYGLTEAFRSTWLDPKFVADHPT
SMGRAIPHAEILVCRGDGTITADEEPGELVHCGPLVAKGYWRDAARTAERFRPAPPASLYGGTAVWSGDRVRRDANGLLY
FVGRDDAMIKTAGNRVSPTEIEEAAVASGLVYEAVAFGVPDARLGAAIILIVRGTHGPADEEGLAAWLRRNLPNFMQPQV
IEWRDALPRNPNGKLDRVAIAADWTRVMAA

Sequences:

>Translated_510_residues
MTTPENPVSQPIDHLARRGAPDAAALLIGERVVTYAELDAGVGLLAAWLREQAGGPGERVASWSAKTRLACLMPLAAARA
GLIHVPINPLLKGPQAQHILADSGAKLLLANAARAESLGDGRPAGCALHDLNIAEEAVDSGGKTLPPSKADPDDLAAILY
TSGSTGRPKGVMLSHANLWLGAESVATYLKLMPVDRVLGVLPLSFDYGQNQLLSSWYAGAAVAPLDYLTARDVVKAVARH
DITTLAGVPPLWVQLVEADWPADTAALLKRLTNSGGALTPSLIEAMRRTFPAADIYPMYGLTEAFRSTWLDPKFVADHPT
SMGRAIPHAEILVCRGDGTITADEEPGELVHCGPLVAKGYWRDAARTAERFRPAPPASLYGGTAVWSGDRVRRDANGLLY
FVGRDDAMIKTAGNRVSPTEIEEAAVASGLVYEAVAFGVPDARLGAAIILIVRGTHGPADEEGLAAWLRRNLPNFMQPQV
IEWRDALPRNPNGKLDRVAIAADWTRVMAA
>Mature_509_residues
TTPENPVSQPIDHLARRGAPDAAALLIGERVVTYAELDAGVGLLAAWLREQAGGPGERVASWSAKTRLACLMPLAAARAG
LIHVPINPLLKGPQAQHILADSGAKLLLANAARAESLGDGRPAGCALHDLNIAEEAVDSGGKTLPPSKADPDDLAAILYT
SGSTGRPKGVMLSHANLWLGAESVATYLKLMPVDRVLGVLPLSFDYGQNQLLSSWYAGAAVAPLDYLTARDVVKAVARHD
ITTLAGVPPLWVQLVEADWPADTAALLKRLTNSGGALTPSLIEAMRRTFPAADIYPMYGLTEAFRSTWLDPKFVADHPTS
MGRAIPHAEILVCRGDGTITADEEPGELVHCGPLVAKGYWRDAARTAERFRPAPPASLYGGTAVWSGDRVRRDANGLLYF
VGRDDAMIKTAGNRVSPTEIEEAAVASGLVYEAVAFGVPDARLGAAIILIVRGTHGPADEEGLAAWLRRNLPNFMQPQVI
EWRDALPRNPNGKLDRVAIAADWTRVMAA

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=546, Percent_Identity=24.1758241758242, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI42544132, Length=453, Percent_Identity=24.0618101545254, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI38505220, Length=377, Percent_Identity=24.1379310344828, Blast_Score=100, Evalue=5e-21,
Organism=Homo sapiens, GI187761345, Length=538, Percent_Identity=25.4646840148699, Blast_Score=100, Evalue=5e-21,
Organism=Homo sapiens, GI187761343, Length=538, Percent_Identity=25.4646840148699, Blast_Score=100, Evalue=5e-21,
Organism=Homo sapiens, GI157311624, Length=470, Percent_Identity=24.6808510638298, Blast_Score=97, Evalue=5e-20,
Organism=Homo sapiens, GI157311622, Length=470, Percent_Identity=24.6808510638298, Blast_Score=97, Evalue=5e-20,
Organism=Homo sapiens, GI58082049, Length=470, Percent_Identity=23.8297872340426, Blast_Score=90, Evalue=5e-18,
Organism=Homo sapiens, GI4758332, Length=381, Percent_Identity=26.509186351706, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI12669909, Length=381, Percent_Identity=26.509186351706, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI115511026, Length=515, Percent_Identity=22.3300970873786, Blast_Score=83, Evalue=5e-16,
Organism=Homo sapiens, GI195947378, Length=373, Percent_Identity=25.4691689008043, Blast_Score=83, Evalue=6e-16,
Organism=Homo sapiens, GI122937307, Length=352, Percent_Identity=23.8636363636364, Blast_Score=83, Evalue=7e-16,
Organism=Escherichia coli, GI1788107, Length=524, Percent_Identity=27.4809160305344, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI1786801, Length=518, Percent_Identity=27.4131274131274, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI145693145, Length=531, Percent_Identity=25.0470809792844, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI1786810, Length=514, Percent_Identity=24.124513618677, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1790505, Length=528, Percent_Identity=23.8636363636364, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1789201, Length=367, Percent_Identity=27.5204359673025, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1788595, Length=361, Percent_Identity=26.5927977839335, Blast_Score=70, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17531443, Length=516, Percent_Identity=24.6124031007752, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI32563687, Length=500, Percent_Identity=25.2, Blast_Score=115, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI17558820, Length=499, Percent_Identity=25.8517034068136, Blast_Score=115, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI71994694, Length=535, Percent_Identity=23.1775700934579, Blast_Score=111, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI71994703, Length=535, Percent_Identity=23.1775700934579, Blast_Score=111, Evalue=8e-25,
Organism=Caenorhabditis elegans, GI71994690, Length=535, Percent_Identity=23.1775700934579, Blast_Score=111, Evalue=8e-25,
Organism=Caenorhabditis elegans, GI17557194, Length=363, Percent_Identity=25.068870523416, Blast_Score=104, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI17560308, Length=528, Percent_Identity=24.0530303030303, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17538037, Length=369, Percent_Identity=25.4742547425474, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17559526, Length=394, Percent_Identity=26.1421319796954, Blast_Score=86, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI71985884, Length=391, Percent_Identity=21.9948849104859, Blast_Score=72, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6319699, Length=367, Percent_Identity=25.8855585831063, Blast_Score=108, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6323182, Length=564, Percent_Identity=23.758865248227, Blast_Score=81, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24581924, Length=524, Percent_Identity=26.7175572519084, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI18859661, Length=526, Percent_Identity=26.615969581749, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI21355181, Length=382, Percent_Identity=24.3455497382199, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI21356947, Length=475, Percent_Identity=23.3684210526316, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020459
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 54232; Mature: 54100

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTPENPVSQPIDHLARRGAPDAAALLIGERVVTYAELDAGVGLLAAWLREQAGGPGERV
CCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHH
ASWSAKTRLACLMPLAAARAGLIHVPINPLLKGPQAQHILADSGAKLLLANAARAESLGD
HHCCHHHHHHHHHHHHHHHCCEEEECCHHHHCCCCCCEEECCCCCEEEEECCHHHHHCCC
GRPAGCALHDLNIAEEAVDSGGKTLPPSKADPDDLAAILYTSGSTGRPKGVMLSHANLWL
CCCCCEEEECCHHHHHHHHCCCCCCCCCCCCHHHEEEEEEECCCCCCCCCEEEECCCEEE
GAESVATYLKLMPVDRVLGVLPLSFDYGQNQLLSSWYAGAAVAPLDYLTARDVVKAVARH
CHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHCCCHHCHHHHHHHHHHHHHHHHH
DITTLAGVPPLWVQLVEADWPADTAALLKRLTNSGGALTPSLIEAMRRTFPAADIYPMYG
CHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCHHHH
LTEAFRSTWLDPKFVADHPTSMGRAIPHAEILVCRGDGTITADEEPGELVHCGPLVAKGY
HHHHHHHHCCCCCEECCCCHHHCCCCCCCEEEEECCCCCEECCCCCCCEEECCHHHHHHH
WRDAARTAERFRPAPPASLYGGTAVWSGDRVRRDANGLLYFVGRDDAMIKTAGNRVSPTE
HHHHHHHHHHCCCCCCCHHCCCCEEECCCHHEECCCCEEEEEECCCEEEEECCCCCCCHH
IEEAAVASGLVYEAVAFGVPDARLGAAIILIVRGTHGPADEEGLAAWLRRNLPNFMQPQV
HHHHHHHHHHHHHHHHHCCCCHHCCEEEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCH
IEWRDALPRNPNGKLDRVAIAADWTRVMAA
HHHHHCCCCCCCCCCEEEEEEEHHHHHHCC
>Mature Secondary Structure 
TTPENPVSQPIDHLARRGAPDAAALLIGERVVTYAELDAGVGLLAAWLREQAGGPGERV
CCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHH
ASWSAKTRLACLMPLAAARAGLIHVPINPLLKGPQAQHILADSGAKLLLANAARAESLGD
HHCCHHHHHHHHHHHHHHHCCEEEECCHHHHCCCCCCEEECCCCCEEEEECCHHHHHCCC
GRPAGCALHDLNIAEEAVDSGGKTLPPSKADPDDLAAILYTSGSTGRPKGVMLSHANLWL
CCCCCEEEECCHHHHHHHHCCCCCCCCCCCCHHHEEEEEEECCCCCCCCCEEEECCCEEE
GAESVATYLKLMPVDRVLGVLPLSFDYGQNQLLSSWYAGAAVAPLDYLTARDVVKAVARH
CHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHCCCHHCHHHHHHHHHHHHHHHHH
DITTLAGVPPLWVQLVEADWPADTAALLKRLTNSGGALTPSLIEAMRRTFPAADIYPMYG
CHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCHHHH
LTEAFRSTWLDPKFVADHPTSMGRAIPHAEILVCRGDGTITADEEPGELVHCGPLVAKGY
HHHHHHHHCCCCCEECCCCHHHCCCCCCCEEEEECCCCCEECCCCCCCEEECCHHHHHHH
WRDAARTAERFRPAPPASLYGGTAVWSGDRVRRDANGLLYFVGRDDAMIKTAGNRVSPTE
HHHHHHHHHHCCCCCCCHHCCCCEEECCCHHEECCCCEEEEEECCCEEEEECCCCCCCHH
IEEAAVASGLVYEAVAFGVPDARLGAAIILIVRGTHGPADEEGLAAWLRRNLPNFMQPQV
HHHHHHHHHHHHHHHHHCCCCHHCCEEEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCH
IEWRDALPRNPNGKLDRVAIAADWTRVMAA
HHHHHCCCCCCCCCCEEEEEEEHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]