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The map label for this gene is murE [H]

Identifier: 103487370

GI number: 103487370

Start: 1985054

End: 1986493

Strand: Reverse

Name: murE [H]

Synonym: Sala_1886

Alternate gene names: 103487370

Gene position: 1986493-1985054 (Counterclockwise)

Preceding gene: 103487371

Following gene: 103487369

Centisome position: 59.38

GC content: 69.51

Gene sequence:

>1440_bases
ATGCGTCTCGCCGCGCTGCTGGACGATGCCGACTGGGCGGGGCCCAATCCCGTCGTCACCGGGCTTGCCATCGATCATCG
CAAAGTGGCGCCGGGAACACTTTTCGGCGCCTTCGTCGGTGAACGGTTCAACGGCGAGGATTTCATTCCCGACGCGGTGG
CGGCGGGCGCGGTCGCGGTCGTCGCGCGGCCTGACGCGAAGGTTGACGGTGCGGTCCATGTCGCCGACGCCAATCCGCGC
CGCGCCTTTGCGCATATCGCCGCGCGCTTTTTTCATCGCTTTCCGGCAACCTGCGTTGCGGTGACGGGCACCAACGGCAA
GACATCGACGGTCGAAATGACGCGCCAATTGTGGCGCATGGCGGGCTTCAACGCCGCGTCGATCGGCACACTCGGCATCA
CCACGTCGGTCGACAGCGCCTCGACGGGCCTGACCACGCCCGACATCGTGACCTTCCTCAGCAATATGTCGGGGCTGGCG
GTCGAGGGCGTGACGCACGCGGCGTTCGAGGCATCGAGCCACGGGCTCGACCAGTATCGCACCGAAGGGCTTCCGGTGAA
GGCGGCGGCGTTCACCAACCTCAGCCACGACCATCTCGATTATCACGGGACGATGGAGGCCTATCTCGCCGCCAAGATGC
GGCTGTTCCGCGAGGTTGTCCAGCCGGGCGGCGCGGCCATCGTCTGGGCCGACGATGCCCATTCGGCGGCGGTTATCGAG
GCGGCGCGCGCTCGCGATGTCCGGGTACTGACGGTCGGGCAGGCGGGCGAGGCGCTGCGTCTCGTGTCGCGCACGCCGAC
GCAGCTTGGCCAGTTGCTCGTCATCGCGGCGGGCGATGTCACGCACAAGGTCACGCTGCCGCTGATCGGCGCCTATCAGG
CGGCGAACGCGCTCGTTGCCGCGGGCCTCGTCATCGCGACGGGCGGCGACGTGGCGCTGACGCTTGCCAATCTCGCGCGG
CTCCAGCCGGTGCGCGGGCGGCTCGAACGGGCCGCCATCGCACGGTCGGGCGCCCCCATCTATGTCGATTATGCGCATAC
CCCTGATGCAATCGAGGCGGCGCTCGACGCGCTGCGCCCGCACGCCACCGGCCGGCTGATCCTCGTCTTTGGTGCCGGCG
GCGACCGCGATCAGGCGAAGCGGCCGGAGATGGGACGGGTCGCCGCCGCCAAGGCCGACCTGCTGATCATCACCGACGAC
AATCCGCGCGGCGAAGACCCCGCCGTCATTCGCGACGCGATTGCCGCGGCGGCGCCCGGCGCGCAGGTGATCGGCGACCG
CCGCGCCGCCATCGCCGCCGCCATCGCCGCGGCGCGCGCCGACGACATCATCTGCATCGCGGGCAAGGGACACGAACAGG
GACAGATCGTCGGGCGCGGCGACGACATGCGCGTCATTCCTTTCGACGATGTGAGCGTCGCGCGCGAGGAGGCGGCATGA

Upstream 100 bases:

>100_bases
CGCGCGCGGGACCGATGCTTGGCGTCTTCCCTGACCAGAGCCGCGACGTCGACGTGTCCGAACTCACCCCGTTGCTGCGG
AGAAACGAGGAGGCGCCCTG

Downstream 100 bases:

>100_bases
CCACCCCGCTCTGGACCGCGCGCACCATCGCCCAAGCGACTGGCGGAATCGCCAGCGCCGATTTCACCGTGAGCGGCGTC
GCCTTCGATTCGCGCGAAGT

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 479; Mature: 479

Protein sequence:

>479_residues
MRLAALLDDADWAGPNPVVTGLAIDHRKVAPGTLFGAFVGERFNGEDFIPDAVAAGAVAVVARPDAKVDGAVHVADANPR
RAFAHIAARFFHRFPATCVAVTGTNGKTSTVEMTRQLWRMAGFNAASIGTLGITTSVDSASTGLTTPDIVTFLSNMSGLA
VEGVTHAAFEASSHGLDQYRTEGLPVKAAAFTNLSHDHLDYHGTMEAYLAAKMRLFREVVQPGGAAIVWADDAHSAAVIE
AARARDVRVLTVGQAGEALRLVSRTPTQLGQLLVIAAGDVTHKVTLPLIGAYQAANALVAAGLVIATGGDVALTLANLAR
LQPVRGRLERAAIARSGAPIYVDYAHTPDAIEAALDALRPHATGRLILVFGAGGDRDQAKRPEMGRVAAAKADLLIITDD
NPRGEDPAVIRDAIAAAAPGAQVIGDRRAAIAAAIAAARADDIICIAGKGHEQGQIVGRGDDMRVIPFDDVSVAREEAA

Sequences:

>Translated_479_residues
MRLAALLDDADWAGPNPVVTGLAIDHRKVAPGTLFGAFVGERFNGEDFIPDAVAAGAVAVVARPDAKVDGAVHVADANPR
RAFAHIAARFFHRFPATCVAVTGTNGKTSTVEMTRQLWRMAGFNAASIGTLGITTSVDSASTGLTTPDIVTFLSNMSGLA
VEGVTHAAFEASSHGLDQYRTEGLPVKAAAFTNLSHDHLDYHGTMEAYLAAKMRLFREVVQPGGAAIVWADDAHSAAVIE
AARARDVRVLTVGQAGEALRLVSRTPTQLGQLLVIAAGDVTHKVTLPLIGAYQAANALVAAGLVIATGGDVALTLANLAR
LQPVRGRLERAAIARSGAPIYVDYAHTPDAIEAALDALRPHATGRLILVFGAGGDRDQAKRPEMGRVAAAKADLLIITDD
NPRGEDPAVIRDAIAAAAPGAQVIGDRRAAIAAAIAAARADDIICIAGKGHEQGQIVGRGDDMRVIPFDDVSVAREEAA
>Mature_479_residues
MRLAALLDDADWAGPNPVVTGLAIDHRKVAPGTLFGAFVGERFNGEDFIPDAVAAGAVAVVARPDAKVDGAVHVADANPR
RAFAHIAARFFHRFPATCVAVTGTNGKTSTVEMTRQLWRMAGFNAASIGTLGITTSVDSASTGLTTPDIVTFLSNMSGLA
VEGVTHAAFEASSHGLDQYRTEGLPVKAAAFTNLSHDHLDYHGTMEAYLAAKMRLFREVVQPGGAAIVWADDAHSAAVIE
AARARDVRVLTVGQAGEALRLVSRTPTQLGQLLVIAAGDVTHKVTLPLIGAYQAANALVAAGLVIATGGDVALTLANLAR
LQPVRGRLERAAIARSGAPIYVDYAHTPDAIEAALDALRPHATGRLILVFGAGGDRDQAKRPEMGRVAAAKADLLIITDD
NPRGEDPAVIRDAIAAAAPGAQVIGDRRAAIAAAIAAARADDIICIAGKGHEQGQIVGRGDDMRVIPFDDVSVAREEAA

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=482, Percent_Identity=38.1742738589212, Blast_Score=235, Evalue=5e-63,
Organism=Escherichia coli, GI1786274, Length=131, Percent_Identity=34.3511450381679, Blast_Score=66, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 49762; Mature: 49762

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLAALLDDADWAGPNPVVTGLAIDHRKVAPGTLFGAFVGERFNGEDFIPDAVAAGAVAV
CCCCEEECCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHCCCCCCCCCCCHHHHCCEEEE
VARPDAKVDGAVHVADANPRRAFAHIAARFFHRFPATCVAVTGTNGKTSTVEMTRQLWRM
EECCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHH
AGFNAASIGTLGITTSVDSASTGLTTPDIVTFLSNMSGLAVEGVTHAAFEASSHGLDQYR
HCCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHCCCCEEECCCHHHHHHCCCCCCHHHH
TEGLPVKAAAFTNLSHDHLDYHGTMEAYLAAKMRLFREVVQPGGAAIVWADDAHSAAVIE
CCCCCEEEHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHH
AARARDVRVLTVGQAGEALRLVSRTPTQLGQLLVIAAGDVTHKVTLPLIGAYQAANALVA
HHCCCCEEEEEECCCCCHHHHHHCCHHHHCCEEEEEECCCEEEEEECCHHHHHHHHHHHH
AGLVIATGGDVALTLANLARLQPVRGRLERAAIARSGAPIYVDYAHTPDAIEAALDALRP
CCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCC
HATGRLILVFGAGGDRDQAKRPEMGRVAAAKADLLIITDDNPRGEDPAVIRDAIAAAAPG
CCCCCEEEEECCCCCCCCCCCCCCCCEEECCCCEEEEECCCCCCCCHHHHHHHHHHCCCC
AQVIGDRRAAIAAAIAAARADDIICIAGKGHEQGQIVGRGDDMRVIPFDDVSVAREEAA
HHHHCCHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEECCCCEEEEECCCHHHHHHHCC
>Mature Secondary Structure
MRLAALLDDADWAGPNPVVTGLAIDHRKVAPGTLFGAFVGERFNGEDFIPDAVAAGAVAV
CCCCEEECCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHCCCCCCCCCCCHHHHCCEEEE
VARPDAKVDGAVHVADANPRRAFAHIAARFFHRFPATCVAVTGTNGKTSTVEMTRQLWRM
EECCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHH
AGFNAASIGTLGITTSVDSASTGLTTPDIVTFLSNMSGLAVEGVTHAAFEASSHGLDQYR
HCCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHCCCCEEECCCHHHHHHCCCCCCHHHH
TEGLPVKAAAFTNLSHDHLDYHGTMEAYLAAKMRLFREVVQPGGAAIVWADDAHSAAVIE
CCCCCEEEHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHH
AARARDVRVLTVGQAGEALRLVSRTPTQLGQLLVIAAGDVTHKVTLPLIGAYQAANALVA
HHCCCCEEEEEECCCCCHHHHHHCCHHHHCCEEEEEECCCEEEEEECCHHHHHHHHHHHH
AGLVIATGGDVALTLANLARLQPVRGRLERAAIARSGAPIYVDYAHTPDAIEAALDALRP
CCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCC
HATGRLILVFGAGGDRDQAKRPEMGRVAAAKADLLIITDDNPRGEDPAVIRDAIAAAAPG
CCCCCEEEEECCCCCCCCCCCCCCCCEEECCCCEEEEECCCCCCCCHHHHHHHHHHCCCC
AQVIGDRRAAIAAAIAAARADDIICIAGKGHEQGQIVGRGDDMRVIPFDDVSVAREEAA
HHHHCCHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEECCCCEEEEECCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA