| Definition | Sphingopyxis alaskensis RB2256, complete genome. |
|---|---|
| Accession | NC_008048 |
| Length | 3,345,170 |
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The map label for this gene is murF [H]
Identifier: 103487369
GI number: 103487369
Start: 1983678
End: 1985057
Strand: Reverse
Name: murF [H]
Synonym: Sala_1885
Alternate gene names: 103487369
Gene position: 1985057-1983678 (Counterclockwise)
Preceding gene: 103487370
Following gene: 103487368
Centisome position: 59.34
GC content: 67.61
Gene sequence:
>1380_bases ATGACCACCCCGCTCTGGACCGCGCGCACCATCGCCCAAGCGACTGGCGGAATCGCCAGCGCCGATTTCACCGTGAGCGG CGTCGCCTTCGATTCGCGCGAAGTCACCAGGGGCGATCTCTTCATCGCGATGAAGGGCGAGAATGCCGACGGGCACAGCT TCATCGACAAGGCGATTGCCGCGGGCGCCGCGGGCATTGTCTGCGAAACGGCGATCGACCATCCGCATGTGCGCGTTGCC GACAGCGCCGCGGCGCTGAACGCTCTGGGCACCGCGTCGCGTGCGCGCAGCCACGGCCGCATCATCGGCGTCACGGGGTC GGCGGGCAAGACGGGGACAAAGGAAGCGCTGTTCGCGGCGCTCGACCGCTTCCGGCCCGGCAAGGCGCATCGCTCGGTCA AAAGCTACAACAATCATGTCGGCGTTCCCTTGAGCCTCGCGCGGATGCCGTCGACCGCCGATTTCGGCATATTCGAGATG GGGATGAACCATGCGGGCGAACTCGCCGCGCTGACCCGCATCGTGCGCCCGCATGTCGCCATCGTCACCACCATCGCGCC CGCGCACATGGAGTTTTTTGGCAGCGAGGAAGCGATTGCCGACGCAAAGGGCGAGATTTTCGAGGGGCTGGAGCCCGGCG GAACCGCGATCATCCCCTTCGACAGCCCGCATTATGCGCGCCTTCGCGCCAAGGCCGAGCAACATGCGGCGCATATCGTC AGCTTCGGGCTGGCCGAGGGCGCCGACGTGCGCGCGGTCGACTGGCTTCCCGACGGGAAGGGCGGCTCGCTCGTCACCGC CCAGGTGCAGGACGCGCTCCTTTGCTTCACCATCGCGCAGGCGGGGGCACATTGGGTCGCCAATTCGCTCGCGGTGCTGG CGGCGGTGAAGGCGGTCGGCGGCGACCTTCCCGCGGCGGGGCTTGCCTTTGCCGAAATGCCGGGCCTCACCGGACGCGGC GCGCGCCATCGGATCGCGGTGCCCGGCGGGCATATCCTCGTCATCGACGAAAGCTATAATGCCAATCCCGCCTCGATGGC GGCGACGATCGGTCAGCTCGCAGGCGAATCCGCCGACCGTAAGGTGGCGATTCTGGGCGCGATGAAGGAGCTTGGACCGG GCGGCGAGGCCTATCATGCGGGCCTCGCGGCTCCGCTCGTCGCAGCAGGCGTGCAATTCGCCCTGCTAGTCGGCGAAGAG ATGGCGCCGCTCGCCAAAGCGCTTGAGGGACGCATCGATTTCGCGCATGTGCCCGCGCACCCCGACGCCACGGCGCGACT GGGCGGCCTCATCCGTCCCGGCGATGCAGTGCTCGTCAAGGGATCGAACAGCGTCGGCCTGTCGCATGTCGTGACGGCGC TGATCAACGGGGATTATTGA
Upstream 100 bases:
>100_bases TCGCGGGCAAGGGACACGAACAGGGACAGATCGTCGGGCGCGGCGACGACATGCGCGTCATTCCTTTCGACGATGTGAGC GTCGCGCGCGAGGAGGCGGC
Downstream 100 bases:
>100_bases TGTTATACTGGCTGGCGGAATGGCTTGGCTTTCCGGGGGCGCTCAATCTCATCCGCTACCTGAGCTTCCGCTCGGGCGCT GCGGTTGCGACCGCGATGAT
Product: UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate--D-alanyl-D-alanyl ligase
Products: NA
Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]
Number of amino acids: Translated: 459; Mature: 458
Protein sequence:
>459_residues MTTPLWTARTIAQATGGIASADFTVSGVAFDSREVTRGDLFIAMKGENADGHSFIDKAIAAGAAGIVCETAIDHPHVRVA DSAAALNALGTASRARSHGRIIGVTGSAGKTGTKEALFAALDRFRPGKAHRSVKSYNNHVGVPLSLARMPSTADFGIFEM GMNHAGELAALTRIVRPHVAIVTTIAPAHMEFFGSEEAIADAKGEIFEGLEPGGTAIIPFDSPHYARLRAKAEQHAAHIV SFGLAEGADVRAVDWLPDGKGGSLVTAQVQDALLCFTIAQAGAHWVANSLAVLAAVKAVGGDLPAAGLAFAEMPGLTGRG ARHRIAVPGGHILVIDESYNANPASMAATIGQLAGESADRKVAILGAMKELGPGGEAYHAGLAAPLVAAGVQFALLVGEE MAPLAKALEGRIDFAHVPAHPDATARLGGLIRPGDAVLVKGSNSVGLSHVVTALINGDY
Sequences:
>Translated_459_residues MTTPLWTARTIAQATGGIASADFTVSGVAFDSREVTRGDLFIAMKGENADGHSFIDKAIAAGAAGIVCETAIDHPHVRVA DSAAALNALGTASRARSHGRIIGVTGSAGKTGTKEALFAALDRFRPGKAHRSVKSYNNHVGVPLSLARMPSTADFGIFEM GMNHAGELAALTRIVRPHVAIVTTIAPAHMEFFGSEEAIADAKGEIFEGLEPGGTAIIPFDSPHYARLRAKAEQHAAHIV SFGLAEGADVRAVDWLPDGKGGSLVTAQVQDALLCFTIAQAGAHWVANSLAVLAAVKAVGGDLPAAGLAFAEMPGLTGRG ARHRIAVPGGHILVIDESYNANPASMAATIGQLAGESADRKVAILGAMKELGPGGEAYHAGLAAPLVAAGVQFALLVGEE MAPLAKALEGRIDFAHVPAHPDATARLGGLIRPGDAVLVKGSNSVGLSHVVTALINGDY >Mature_458_residues TTPLWTARTIAQATGGIASADFTVSGVAFDSREVTRGDLFIAMKGENADGHSFIDKAIAAGAAGIVCETAIDHPHVRVAD SAAALNALGTASRARSHGRIIGVTGSAGKTGTKEALFAALDRFRPGKAHRSVKSYNNHVGVPLSLARMPSTADFGIFEMG MNHAGELAALTRIVRPHVAIVTTIAPAHMEFFGSEEAIADAKGEIFEGLEPGGTAIIPFDSPHYARLRAKAEQHAAHIVS FGLAEGADVRAVDWLPDGKGGSLVTAQVQDALLCFTIAQAGAHWVANSLAVLAAVKAVGGDLPAAGLAFAEMPGLTGRGA RHRIAVPGGHILVIDESYNANPASMAATIGQLAGESADRKVAILGAMKELGPGGEAYHAGLAAPLVAAGVQFALLVGEEM APLAKALEGRIDFAHVPAHPDATARLGGLIRPGDAVLVKGSNSVGLSHVVTALINGDY
Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]
COG id: COG0770
COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase
Gene ontology:
Cell location: Cytoplasm (Probable) [H]
Metaboloic importance: Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the MurCDEF family [H]
Homologues:
Organism=Escherichia coli, GI1786274, Length=453, Percent_Identity=33.5540838852097, Blast_Score=176, Evalue=3e-45,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR004101 - InterPro: IPR013221 - InterPro: IPR000713 - InterPro: IPR005863 [H]
Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]
EC number: =6.3.2.10 [H]
Molecular weight: Translated: 46990; Mature: 46859
Theoretical pI: Translated: 6.74; Mature: 6.74
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.4 %Cys (Translated Protein) 2.2 %Met (Translated Protein) 2.6 %Cys+Met (Translated Protein) 0.4 %Cys (Mature Protein) 2.0 %Met (Mature Protein) 2.4 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MTTPLWTARTIAQATGGIASADFTVSGVAFDSREVTRGDLFIAMKGENADGHSFIDKAIA CCCCCCHHHHHHHHCCCCEECCEEEEEEEECCCCCCCCEEEEEEECCCCCCHHHHHHHHH AGAAGIVCETAIDHPHVRVADSAAALNALGTASRARSHGRIIGVTGSAGKTGTKEALFAA CCCCCEEEECCCCCCCEEEECHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHH LDRFRPGKAHRSVKSYNNHVGVPLSLARMPSTADFGIFEMGMNHAGELAALTRIVRPHVA HHHCCCCHHHHHHHHHCCCCCCCEECCCCCCCCCCCHHHHCCCCCHHHHHHHHHHCCCEE IVTTIAPAHMEFFGSEEAIADAKGEIFEGLEPGGTAIIPFDSPHYARLRAKAEQHAAHIV EEEEECHHHHHHCCCCHHHHCCCHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHH SFGLAEGADVRAVDWLPDGKGGSLVTAQVQDALLCFTIAQAGAHWVANSLAVLAAVKAVG HHHCCCCCCEEEEECCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC GDLPAAGLAFAEMPGLTGRGARHRIAVPGGHILVIDESYNANPASMAATIGQLAGESADR CCCCCCCCHHHHCCCCCCCCCCEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCC KVAILGAMKELGPGGEAYHAGLAAPLVAAGVQFALLVGEEMAPLAKALEGRIDFAHVPAH EEEEEEEHHHCCCCCHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHCCCCEEECCCC PDATARLGGLIRPGDAVLVKGSNSVGLSHVVTALINGDY CCHHHHHCCEECCCCEEEEECCCCCCHHHHHHHHHCCCC >Mature Secondary Structure TTPLWTARTIAQATGGIASADFTVSGVAFDSREVTRGDLFIAMKGENADGHSFIDKAIA CCCCCHHHHHHHHCCCCEECCEEEEEEEECCCCCCCCEEEEEEECCCCCCHHHHHHHHH AGAAGIVCETAIDHPHVRVADSAAALNALGTASRARSHGRIIGVTGSAGKTGTKEALFAA CCCCCEEEECCCCCCCEEEECHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHH LDRFRPGKAHRSVKSYNNHVGVPLSLARMPSTADFGIFEMGMNHAGELAALTRIVRPHVA HHHCCCCHHHHHHHHHCCCCCCCEECCCCCCCCCCCHHHHCCCCCHHHHHHHHHHCCCEE IVTTIAPAHMEFFGSEEAIADAKGEIFEGLEPGGTAIIPFDSPHYARLRAKAEQHAAHIV EEEEECHHHHHHCCCCHHHHCCCHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHH SFGLAEGADVRAVDWLPDGKGGSLVTAQVQDALLCFTIAQAGAHWVANSLAVLAAVKAVG HHHCCCCCCEEEEECCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC GDLPAAGLAFAEMPGLTGRGARHRIAVPGGHILVIDESYNANPASMAATIGQLAGESADR CCCCCCCCHHHHCCCCCCCCCCEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCC KVAILGAMKELGPGGEAYHAGLAAPLVAAGVQFALLVGEEMAPLAKALEGRIDFAHVPAH EEEEEEEHHHCCCCCHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHCCCCEEECCCC PDATARLGGLIRPGDAVLVKGSNSVGLSHVVTALINGDY CCHHHHHCCEECCCCEEEEECCCCCCHHHHHHHHHCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 9823893; 9274032 [H]