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The map label for this gene is murD

Identifier: 103487367

GI number: 103487367

Start: 1981307

End: 1982611

Strand: Reverse

Name: murD

Synonym: Sala_1883

Alternate gene names: 103487367

Gene position: 1982611-1981307 (Counterclockwise)

Preceding gene: 103487368

Following gene: 103487366

Centisome position: 59.27

GC content: 68.28

Gene sequence:

>1305_bases
GTGATTACCTCGCGCGCCTTTGCCGGTCGCCGCTATGCGGTGCTGGGACTGGCGCGCTCGGGGCTGGCCACCGTCGAGGC
GCTTGTCGCCAGCGGCGCGGGCGTCACCGCGTGGGATGACCGCGAGGAGGCGCGCGACGCCGCGATGGCGCTGGGCGCCG
ACATCGGCAATCCGCTGGACATCGACCTCATCGGCTTTGCGGGCGTCGTCGTATCGCCGGGCGTGCCGCTCAACCGCCAC
CCGATCGCCGCGCACGCGCGCGATGCCCATGTCCCCGTCATCGGCGACATCGAGCTGTTTGCCGAAGCGCGCAGCGAGCT
GCCGCCGCACAAGGTCGTCGGCATCACCGGCACCAATGGCAAATCCACCGTCACCGCGCTGATCGCGCACATGCTTGAAA
GCGCGGGCGTACCCGTGCTGATGGGCGGCAATATCGGTCTGCCGATCCTGACGCGCGAACCCCTGCCTGAAGCTGGTGTC
TATGTGCTCGAACTGTCGAGCTTTCAGATTGACCTTGCGCACAGTCTGGCGTGCGACGTCGCGGTGCTCACCAACATCAG
CCCCGATCATCTCGACCGCTACGACGGGTTCGAGGGTTATGTCGCATCGAAGGCGCGGCTGTTCAGCCTGCAACACCGCG
ATCAGGTCGCGATCATCGCGACCGACGACGATCCTTCAAAAATGATCGCAAGCCGGGTGAACCATCGCCTTCACCGCGTT
TCGGCGAAGGACATCGACCCCGTGGATCAGGCGCGCTGGCCCGCGCTGCAAGGCCCCCACAATGCGCAGAACGCCGTTTG
CGCGATCGCCGCGTGTCGCGTGCTGGGGCTGGACGACGAAGCAATCGAACGCGGCCTCGCGACCTTCCGGTCGCTCCCGC
ACCGCATGGAATTGGTCGGCGAAGCCCGCGGCGCCCGCTGGTATAATGACAGCAAGGCGACCAACGCGGCTTCCGCTGCA
CCGGCGCTGGCGGCGTTTCCGCCGGCGCCGGACCAGCGTTTGCACTGGATTGCGGGGGGACAGGCGAAGGGCGACGGGCT
GGCGGCGTGCCGGCCGTGGTTCGGCCATGTCAAACGCGCCTATCTGATCGGCGAGGCGATGGCGCCGTTCGCGGCGGAAA
TCGGCGACGCCATCGCGATCGAGCGATCGGGCGACCTGGCGAGTGCTGTGGCGCAGGCGGCGGCGGCGGTCCAGCCCGGC
GACATCGTGCTGCTGTCGCCCGCCTGCGCGTCGTTCGACCAGTTCAAGGATTATGAGGCGCGCGGCGAGGCGTTCCGCGC
AGCGGTCGAGGCGCTGGGGGCATGA

Upstream 100 bases:

>100_bases
ATTTCGAGCAGCTCGGCTGGCCCGAATCCACCGTCGTCATTCGCTTCTGGATCGTCTCGATCGTCCTGGCGCTTGCGGGA
CTCGCGACCTTGAAGCTGCG

Downstream 100 bases:

>100_bases
CGGTCGCCTCTTTCCGTTCGCGTCGAGCGAAGTCGAGACGCCCATCGGTCTCATATGCCCTCGGGGCATCTCGACTTCGC
TCGATGCGAGCGGGGAGAGA

Product: UDP-N-acetylmuramoylalanine--D-glutamate ligase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MITSRAFAGRRYAVLGLARSGLATVEALVASGAGVTAWDDREEARDAAMALGADIGNPLDIDLIGFAGVVVSPGVPLNRH
PIAAHARDAHVPVIGDIELFAEARSELPPHKVVGITGTNGKSTVTALIAHMLESAGVPVLMGGNIGLPILTREPLPEAGV
YVLELSSFQIDLAHSLACDVAVLTNISPDHLDRYDGFEGYVASKARLFSLQHRDQVAIIATDDDPSKMIASRVNHRLHRV
SAKDIDPVDQARWPALQGPHNAQNAVCAIAACRVLGLDDEAIERGLATFRSLPHRMELVGEARGARWYNDSKATNAASAA
PALAAFPPAPDQRLHWIAGGQAKGDGLAACRPWFGHVKRAYLIGEAMAPFAAEIGDAIAIERSGDLASAVAQAAAAVQPG
DIVLLSPACASFDQFKDYEARGEAFRAAVEALGA

Sequences:

>Translated_434_residues
MITSRAFAGRRYAVLGLARSGLATVEALVASGAGVTAWDDREEARDAAMALGADIGNPLDIDLIGFAGVVVSPGVPLNRH
PIAAHARDAHVPVIGDIELFAEARSELPPHKVVGITGTNGKSTVTALIAHMLESAGVPVLMGGNIGLPILTREPLPEAGV
YVLELSSFQIDLAHSLACDVAVLTNISPDHLDRYDGFEGYVASKARLFSLQHRDQVAIIATDDDPSKMIASRVNHRLHRV
SAKDIDPVDQARWPALQGPHNAQNAVCAIAACRVLGLDDEAIERGLATFRSLPHRMELVGEARGARWYNDSKATNAASAA
PALAAFPPAPDQRLHWIAGGQAKGDGLAACRPWFGHVKRAYLIGEAMAPFAAEIGDAIAIERSGDLASAVAQAAAAVQPG
DIVLLSPACASFDQFKDYEARGEAFRAAVEALGA
>Mature_434_residues
MITSRAFAGRRYAVLGLARSGLATVEALVASGAGVTAWDDREEARDAAMALGADIGNPLDIDLIGFAGVVVSPGVPLNRH
PIAAHARDAHVPVIGDIELFAEARSELPPHKVVGITGTNGKSTVTALIAHMLESAGVPVLMGGNIGLPILTREPLPEAGV
YVLELSSFQIDLAHSLACDVAVLTNISPDHLDRYDGFEGYVASKARLFSLQHRDQVAIIATDDDPSKMIASRVNHRLHRV
SAKDIDPVDQARWPALQGPHNAQNAVCAIAACRVLGLDDEAIERGLATFRSLPHRMELVGEARGARWYNDSKATNAASAA
PALAAFPPAPDQRLHWIAGGQAKGDGLAACRPWFGHVKRAYLIGEAMAPFAAEIGDAIAIERSGDLASAVAQAAAAVQPG
DIVLLSPACASFDQFKDYEARGEAFRAAVEALGA

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA)

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1786276, Length=451, Percent_Identity=37.0288248337029, Blast_Score=209, Evalue=3e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURD_SPHAL (Q1GRX7)

Other databases:

- EMBL:   CP000356
- RefSeq:   YP_616928.1
- ProteinModelPortal:   Q1GRX7
- SMR:   Q1GRX7
- GeneID:   4082628
- GenomeReviews:   CP000356_GR
- KEGG:   sal:Sala_1883
- NMPDR:   fig|317655.9.peg.1811
- HOGENOM:   HBG750024
- OMA:   VKYYNDS
- ProtClustDB:   CLSK835273
- BioCyc:   SALA317655:SALA_1883-MONOMER
- BRENDA:   6.3.2.9
- GO:   GO:0005737
- HAMAP:   MF_00639
- InterPro:   IPR018109
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01087

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.9

Molecular weight: Translated: 45612; Mature: 45612

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: PS00012 PHOSPHOPANTETHEINE ; PS01011 FOLYLPOLYGLU_SYNT_1 ; PS00237 G_PROTEIN_RECEP_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITSRAFAGRRYAVLGLARSGLATVEALVASGAGVTAWDDREEARDAAMALGADIGNPLD
CCCCCCCCCCCEEEEEEHHCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCE
IDLIGFAGVVVSPGVPLNRHPIAAHARDAHVPVIGDIELFAEARSELPPHKVVGITGTNG
EEEEEECCEEECCCCCCCCCCCCCCCCCCCCCEEECHHHHHHHHHCCCCCEEEEEECCCC
KSTVTALIAHMLESAGVPVLMGGNIGLPILTREPLPEAGVYVLELSSFQIDLAHSLACDV
HHHHHHHHHHHHHHCCCCEEECCCCCCCEEECCCCCCCCEEEEEECCCEEEHHHHHHHCC
AVLTNISPDHLDRYDGFEGYVASKARLFSLQHRDQVAIIATDDDPSKMIASRVNHRLHRV
HHCCCCCHHHHHHCCCCCCHHHHHHHHEEECCCCCEEEEEECCCHHHHHHHHHHHHHHHC
SAKDIDPVDQARWPALQGPHNAQNAVCAIAACRVLGLDDEAIERGLATFRSLPHRMELVG
CCCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHH
EARGARWYNDSKATNAASAAPALAAFPPAPDQRLHWIAGGQAKGDGLAACRPWFGHVKRA
HHCCCCEECCCCCCCCHHCCCCHHCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHH
YLIGEAMAPFAAEIGDAIAIERSGDLASAVAQAAAAVQPGDIVLLSPACASFDQFKDYEA
HHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHCCCCCEEEECCCHHCHHHHHCHHH
RGEAFRAAVEALGA
HHHHHHHHHHHHCC
>Mature Secondary Structure
MITSRAFAGRRYAVLGLARSGLATVEALVASGAGVTAWDDREEARDAAMALGADIGNPLD
CCCCCCCCCCCEEEEEEHHCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCE
IDLIGFAGVVVSPGVPLNRHPIAAHARDAHVPVIGDIELFAEARSELPPHKVVGITGTNG
EEEEEECCEEECCCCCCCCCCCCCCCCCCCCCEEECHHHHHHHHHCCCCCEEEEEECCCC
KSTVTALIAHMLESAGVPVLMGGNIGLPILTREPLPEAGVYVLELSSFQIDLAHSLACDV
HHHHHHHHHHHHHHCCCCEEECCCCCCCEEECCCCCCCCEEEEEECCCEEEHHHHHHHCC
AVLTNISPDHLDRYDGFEGYVASKARLFSLQHRDQVAIIATDDDPSKMIASRVNHRLHRV
HHCCCCCHHHHHHCCCCCCHHHHHHHHEEECCCCCEEEEEECCCHHHHHHHHHHHHHHHC
SAKDIDPVDQARWPALQGPHNAQNAVCAIAACRVLGLDDEAIERGLATFRSLPHRMELVG
CCCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHH
EARGARWYNDSKATNAASAAPALAAFPPAPDQRLHWIAGGQAKGDGLAACRPWFGHVKRA
HHCCCCEECCCCCCCCHHCCCCHHCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHH
YLIGEAMAPFAAEIGDAIAIERSGDLASAVAQAAAAVQPGDIVLLSPACASFDQFKDYEA
HHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHCCCCCEEEECCCHHCHHHHHCHHH
RGEAFRAAVEALGA
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA