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The map label for this gene is ddl

Identifier: 103487362

GI number: 103487362

Start: 1975366

End: 1976292

Strand: Reverse

Name: ddl

Synonym: Sala_1878

Alternate gene names: 103487362

Gene position: 1976292-1975366 (Counterclockwise)

Preceding gene: 103487363

Following gene: 103487361

Centisome position: 59.08

GC content: 64.51

Gene sequence:

>927_bases
ATGAGCCGGGGTCCGTGGCATGTCGCCGTCCTGATGGGCGGCTGGTCGGCCGAGCGCGAGGTGTCGTTGATGAGCGGCAA
GGGCGTGGCCGACGCGCTCGAAAGCCGTGGGCACAAGGTCACGCGTATCGACATGGACCGCGACGTCGCGCTGCGGCTGG
CCGAGGCGAAACCCGATGTCGTGTTCAACGCGCTCCACGGCGTTCCCGGCGAGGACGGCACGGTGCAGGGGATGCTCGAC
CTGATGGGTTTCAGATATACTCACAGCGGGCTCGTCACCTCGGTGATCGCGATCGACAAGGAATTGACGAAGCAGGCGCT
GGTGCCGCATGGAATCCCCATGCCGACGGGAACGATGGTCGACAGCGAAAGCCTCTTTTCGGTCGACCCCTTGCCACGCC
CCTATGTTCTGAAGCCCGTCAATGAAGGCTCGTCGGTCGGGGTCGCGATCGTCAGGGACGACAGCAATTACGGCAATCCC
ATTTCGCGCGATGCACTGGGCCCGTGGCAGCAGTTCGACCGCCTGCTTGCCGAACCGTTCATCAAGGGGCGCGAGCTCAC
CGTCGCGGTGCTGGGCGACACGGCGCTGGCTGTTACCGAACTGCGCGTCAAATCAGGCTTTTACGATTATGACGCCAAAT
ATACCGACGGGCTGACCGAGCATGTCTGCCCCGCCGACGTGCCGGATGACGTCGCGCAGCGGATGAAGGATCTGGCGCTG
CAGGCGCACCGGCTGCTCGGCTGCAAGGGGGCGTCGCGCTCGGATTTTCGCTGGGACGACGAGCACGGCCTTGCGGGGAT
TTTCCTGCTCGAGGTCAATACCCAGCCCGGCATGACGCCGCTCAGCCTCGTCCCCGAACAGGCGCGCGCGGTCGGGATGG
ATTACGCCGAACTGGTCGAACGCATCGTCGAGGAAGCACTGACATGA

Upstream 100 bases:

>100_bases
GCGCCGATATCGAGGCATTGGGCGAAGAAGTCCGCCGCCGGGTGAAGGACAAGAGCGGCATCGAATTGCAGTGGGAGATT
CAGCGCGTGGGTAAGGCCGA

Downstream 100 bases:

>100_bases
GGGGGCGGCCGTGAGCAGTACGAAGATCAGGCGCGCCAAGGCGCCGCCGCGGCGGCCCGCGCGCGCGCCGAAGAAACGGC
GCAAGGTTCAGCAGTCGCGG

Product: D-alanine--D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase

Number of amino acids: Translated: 308; Mature: 307

Protein sequence:

>308_residues
MSRGPWHVAVLMGGWSAEREVSLMSGKGVADALESRGHKVTRIDMDRDVALRLAEAKPDVVFNALHGVPGEDGTVQGMLD
LMGFRYTHSGLVTSVIAIDKELTKQALVPHGIPMPTGTMVDSESLFSVDPLPRPYVLKPVNEGSSVGVAIVRDDSNYGNP
ISRDALGPWQQFDRLLAEPFIKGRELTVAVLGDTALAVTELRVKSGFYDYDAKYTDGLTEHVCPADVPDDVAQRMKDLAL
QAHRLLGCKGASRSDFRWDDEHGLAGIFLLEVNTQPGMTPLSLVPEQARAVGMDYAELVERIVEEALT

Sequences:

>Translated_308_residues
MSRGPWHVAVLMGGWSAEREVSLMSGKGVADALESRGHKVTRIDMDRDVALRLAEAKPDVVFNALHGVPGEDGTVQGMLD
LMGFRYTHSGLVTSVIAIDKELTKQALVPHGIPMPTGTMVDSESLFSVDPLPRPYVLKPVNEGSSVGVAIVRDDSNYGNP
ISRDALGPWQQFDRLLAEPFIKGRELTVAVLGDTALAVTELRVKSGFYDYDAKYTDGLTEHVCPADVPDDVAQRMKDLAL
QAHRLLGCKGASRSDFRWDDEHGLAGIFLLEVNTQPGMTPLSLVPEQARAVGMDYAELVERIVEEALT
>Mature_307_residues
SRGPWHVAVLMGGWSAEREVSLMSGKGVADALESRGHKVTRIDMDRDVALRLAEAKPDVVFNALHGVPGEDGTVQGMLDL
MGFRYTHSGLVTSVIAIDKELTKQALVPHGIPMPTGTMVDSESLFSVDPLPRPYVLKPVNEGSSVGVAIVRDDSNYGNPI
SRDALGPWQQFDRLLAEPFIKGRELTVAVLGDTALAVTELRVKSGFYDYDAKYTDGLTEHVCPADVPDDVAQRMKDLALQ
AHRLLGCKGASRSDFRWDDEHGLAGIFLLEVNTQPGMTPLSLVPEQARAVGMDYAELVERIVEEALT

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786280, Length=310, Percent_Identity=39.0322580645161, Blast_Score=182, Evalue=2e-47,
Organism=Escherichia coli, GI1786579, Length=354, Percent_Identity=31.0734463276836, Blast_Score=146, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDL_SPHAL (Q1GRY2)

Other databases:

- EMBL:   CP000356
- RefSeq:   YP_616923.1
- ProteinModelPortal:   Q1GRY2
- SMR:   Q1GRY2
- GeneID:   4082623
- GenomeReviews:   CP000356_GR
- KEGG:   sal:Sala_1878
- NMPDR:   fig|317655.9.peg.1806
- HOGENOM:   HBG427092
- OMA:   TDGWYDY
- ProtClustDB:   PRK01372
- BioCyc:   SALA317655:SALA_1878-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 33522; Mature: 33390

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRGPWHVAVLMGGWSAEREVSLMSGKGVADALESRGHKVTRIDMDRDVALRLAEAKPDV
CCCCCEEEEEEEECCCCCCEEEEECCCCHHHHHHHCCCEEEEEECCCCHHHEECCCCCCH
VFNALHGVPGEDGTVQGMLDLMGFRYTHSGLVTSVIAIDKELTKQALVPHGIPMPTGTMV
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEE
DSESLFSVDPLPRPYVLKPVNEGSSVGVAIVRDDSNYGNPISRDALGPWQQFDRLLAEPF
CCCCCEECCCCCCCEEEEECCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHH
IKGRELTVAVLGDTALAVTELRVKSGFYDYDAKYTDGLTEHVCPADVPDDVAQRMKDLAL
CCCCEEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCHHCCCCCCCHHHHHHHHHHHHH
QAHRLLGCKGASRSDFRWDDEHGLAGIFLLEVNTQPGMTPLSLVPEQARAVGMDYAELVE
HHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCHHHHCCHHHHHHCCCHHHHHH
RIVEEALT
HHHHHHCC
>Mature Secondary Structure 
SRGPWHVAVLMGGWSAEREVSLMSGKGVADALESRGHKVTRIDMDRDVALRLAEAKPDV
CCCCEEEEEEEECCCCCCEEEEECCCCHHHHHHHCCCEEEEEECCCCHHHEECCCCCCH
VFNALHGVPGEDGTVQGMLDLMGFRYTHSGLVTSVIAIDKELTKQALVPHGIPMPTGTMV
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEE
DSESLFSVDPLPRPYVLKPVNEGSSVGVAIVRDDSNYGNPISRDALGPWQQFDRLLAEPF
CCCCCEECCCCCCCEEEEECCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHH
IKGRELTVAVLGDTALAVTELRVKSGFYDYDAKYTDGLTEHVCPADVPDDVAQRMKDLAL
CCCCEEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCHHCCCCCCCHHHHHHHHHHHHH
QAHRLLGCKGASRSDFRWDDEHGLAGIFLLEVNTQPGMTPLSLVPEQARAVGMDYAELVE
HHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCHHHHCCHHHHHHCCCHHHHHH
RIVEEALT
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA