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The map label for this gene is ntrC [H]

Identifier: 103486761

GI number: 103486761

Start: 1332420

End: 1333838

Strand: Reverse

Name: ntrC [H]

Synonym: Sala_1275

Alternate gene names: 103486761

Gene position: 1333838-1332420 (Counterclockwise)

Preceding gene: 103486762

Following gene: 103486760

Centisome position: 39.87

GC content: 68.15

Gene sequence:

>1419_bases
ATGAGCGAAGGCAAGACGATCCTGCTGGTCGAGGACGATCCGGCGATCGCGATGGTCATCCGCGAAACGCTCGACGGCGA
ATGCGGCCTTCTCGCGTCGGTCGGCAGCATCGCCGAACGCAACGCCTGGCTCGCCGCTCACCGCGCCGACCTCATCATCA
CCGATGTTGTCCTGCCCGATGGCGACGGCATCGACTCGCTGGTCCATGCCGGAGTCGATCCTGCAACGCCGGTGATCGTG
CTGTCGGCCCAGAATACGCTCGACACCGCGGTCCGGGCGACGGGCATCGGCAGCTATGATTATCTGCCCAAGCCCTTCGA
CCTCGACGAACTGACCGCCAGCGTCCGCGCCGCGCTGCAACGCCGCGTCGAGGCAGCCGCGAGCGACGCCCCGCCGCCTG
CCGACAGCCACGGCCTCGTCGGCCGCGCGCCGGCGATGCAGGCGGTTTACCGGACGATCGCCCGGCTCGCGACGAACGAT
CTCGCGGTGCTGATCCTCGGCGAGTCGGGGACAGGCAAGGAGGTCGTTGCGCGCGCGATTCACGCCACCGGGCTGCGCCG
CGACGGCCCCTTCGTCGCGATCAACATGGCGGCCATCCCGCGCGAACTGATCGAGGCCGAACTGTTCGGTCATGAAAAGG
GCGCCTTTACGGGCGCGCACAGCCGCAATGCTGGCCGCTTCGAACAGGCGGCGGGCGGAACCTTGTTCCTCGACGAGATC
GGTGACATGCCCCTGGAGGCGCAGACGCGGCTATTGCGCGTGCTGCAAAGCAACGAGTATTCGACCGTCGGCGGCAGCCA
GGCGCTCCGCGCCGATGTGCGCGTGATCGCCGCGACGCACCGCGACATGCGCACGCTCGTCGCCGACGGTCGCTTTCGCG
AAGATCTCTTTTACCGGCTCAACGTCATCCCTGTGACCTTGCCGCCGCTGCGCGACCGCCGCAGCGACATTGCTGCGCTC
GTTCGCCATTTTGTCGAGGTCGGGCGCGGATCGGGGCTTCCCGACCGGCAGTTCGCGCCCGAAGCGATGCAATGGCTCGA
ACGCCACGACTGGCCGGGTAATGTCCGTGAGCTGGGCAATGTGGTGCAGCGACTCGCGGTGCTGTCGCGCGATACCGTCG
TCACCGCACGCGAGGTCGAGATGGTGCTGCGCGACAATGACGAAAGCGGCGGCGTGGCCGATCCCGCGGCACTGATCGCG
CGCGCCGTCGACGACTGGGCGCGCGAAAAGATGGCGAGCGCCGAACCCGACGGCGGGATTCACGGCCAGCTCGAGGCGAT
CGTCGAGGCCGCACTGTTCCGCCGCGTGCTGCGCGATGTGCGCGGCAACCAGCTGGAGGCGGCGCGGCGTCTCGGCATCA
ACCGTAACACGCTGCGCAAGCGCCTCGCTCAGCTGGAAATCGATCCGCTGCATCCCTGA

Upstream 100 bases:

>100_bases
AGCTGGCGCGCGACATGGGCGGGACGGTGCAGCATATGCGCGATGGCGACTGGACACGCTTTCGCGTCCACCTGCCGGTC
GCGCAAGCAAAGGGACGGGG

Downstream 100 bases:

>100_bases
AGCGCAGCCTGTTCGCGGCGAACGGGCATGGCGGGCGCGATTGTGTGTTTTCTATGCAACAGGAATGTCGTAATGAAGCA
ACAATGACGCCTGTGGCTGC

Product: two component, sigma54 specific, Fis family transcriptional regulator

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MSEGKTILLVEDDPAIAMVIRETLDGECGLLASVGSIAERNAWLAAHRADLIITDVVLPDGDGIDSLVHAGVDPATPVIV
LSAQNTLDTAVRATGIGSYDYLPKPFDLDELTASVRAALQRRVEAAASDAPPPADSHGLVGRAPAMQAVYRTIARLATND
LAVLILGESGTGKEVVARAIHATGLRRDGPFVAINMAAIPRELIEAELFGHEKGAFTGAHSRNAGRFEQAAGGTLFLDEI
GDMPLEAQTRLLRVLQSNEYSTVGGSQALRADVRVIAATHRDMRTLVADGRFREDLFYRLNVIPVTLPPLRDRRSDIAAL
VRHFVEVGRGSGLPDRQFAPEAMQWLERHDWPGNVRELGNVVQRLAVLSRDTVVTAREVEMVLRDNDESGGVADPAALIA
RAVDDWAREKMASAEPDGGIHGQLEAIVEAALFRRVLRDVRGNQLEAARRLGINRNTLRKRLAQLEIDPLHP

Sequences:

>Translated_472_residues
MSEGKTILLVEDDPAIAMVIRETLDGECGLLASVGSIAERNAWLAAHRADLIITDVVLPDGDGIDSLVHAGVDPATPVIV
LSAQNTLDTAVRATGIGSYDYLPKPFDLDELTASVRAALQRRVEAAASDAPPPADSHGLVGRAPAMQAVYRTIARLATND
LAVLILGESGTGKEVVARAIHATGLRRDGPFVAINMAAIPRELIEAELFGHEKGAFTGAHSRNAGRFEQAAGGTLFLDEI
GDMPLEAQTRLLRVLQSNEYSTVGGSQALRADVRVIAATHRDMRTLVADGRFREDLFYRLNVIPVTLPPLRDRRSDIAAL
VRHFVEVGRGSGLPDRQFAPEAMQWLERHDWPGNVRELGNVVQRLAVLSRDTVVTAREVEMVLRDNDESGGVADPAALIA
RAVDDWAREKMASAEPDGGIHGQLEAIVEAALFRRVLRDVRGNQLEAARRLGINRNTLRKRLAQLEIDPLHP
>Mature_471_residues
SEGKTILLVEDDPAIAMVIRETLDGECGLLASVGSIAERNAWLAAHRADLIITDVVLPDGDGIDSLVHAGVDPATPVIVL
SAQNTLDTAVRATGIGSYDYLPKPFDLDELTASVRAALQRRVEAAASDAPPPADSHGLVGRAPAMQAVYRTIARLATNDL
AVLILGESGTGKEVVARAIHATGLRRDGPFVAINMAAIPRELIEAELFGHEKGAFTGAHSRNAGRFEQAAGGTLFLDEIG
DMPLEAQTRLLRVLQSNEYSTVGGSQALRADVRVIAATHRDMRTLVADGRFREDLFYRLNVIPVTLPPLRDRRSDIAALV
RHFVEVGRGSGLPDRQFAPEAMQWLERHDWPGNVRELGNVVQRLAVLSRDTVVTAREVEMVLRDNDESGGVADPAALIAR
AVDDWAREKMASAEPDGGIHGQLEAIVEAALFRRVLRDVRGNQLEAARRLGINRNTLRKRLAQLEIDPLHP

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=470, Percent_Identity=44.0425531914894, Blast_Score=350, Evalue=2e-97,
Organism=Escherichia coli, GI1788550, Length=430, Percent_Identity=40.4651162790698, Blast_Score=311, Evalue=6e-86,
Organism=Escherichia coli, GI1790437, Length=465, Percent_Identity=40.2150537634409, Blast_Score=311, Evalue=6e-86,
Organism=Escherichia coli, GI1788905, Length=467, Percent_Identity=37.0449678800857, Blast_Score=272, Evalue=4e-74,
Organism=Escherichia coli, GI87082117, Length=339, Percent_Identity=44.2477876106195, Blast_Score=252, Evalue=4e-68,
Organism=Escherichia coli, GI1789087, Length=334, Percent_Identity=42.5149700598802, Blast_Score=248, Evalue=6e-67,
Organism=Escherichia coli, GI1789233, Length=251, Percent_Identity=48.207171314741, Blast_Score=245, Evalue=4e-66,
Organism=Escherichia coli, GI87082152, Length=235, Percent_Identity=49.3617021276596, Blast_Score=220, Evalue=2e-58,
Organism=Escherichia coli, GI1786524, Length=246, Percent_Identity=48.780487804878, Blast_Score=213, Evalue=3e-56,
Organism=Escherichia coli, GI87081872, Length=229, Percent_Identity=46.7248908296943, Blast_Score=204, Evalue=1e-53,
Organism=Escherichia coli, GI1787583, Length=227, Percent_Identity=41.8502202643172, Blast_Score=175, Evalue=5e-45,
Organism=Escherichia coli, GI1789828, Length=275, Percent_Identity=35.2727272727273, Blast_Score=139, Evalue=4e-34,
Organism=Escherichia coli, GI87081858, Length=319, Percent_Identity=30.0940438871473, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI1786911, Length=129, Percent_Identity=41.0852713178295, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI1790552, Length=126, Percent_Identity=33.3333333333333, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR010114
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 51117; Mature: 50986

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEGKTILLVEDDPAIAMVIRETLDGECGLLASVGSIAERNAWLAAHRADLIITDVVLPD
CCCCCEEEEEECCCHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHCCCEEEEEEEECC
GDGIDSLVHAGVDPATPVIVLSAQNTLDTAVRATGIGSYDYLPKPFDLDELTASVRAALQ
CCCHHHHHHCCCCCCCCEEEEECCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHH
RRVEAAASDAPPPADSHGLVGRAPAMQAVYRTIARLATNDLAVLILGESGTGKEVVARAI
HHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHH
HATGLRRDGPFVAINMAAIPRELIEAELFGHEKGAFTGAHSRNAGRFEQAAGGTLFLDEI
HHHCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCEEEHHHH
GDMPLEAQTRLLRVLQSNEYSTVGGSQALRADVRVIAATHRDMRTLVADGRFREDLFYRL
CCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHEEE
NVIPVTLPPLRDRRSDIAALVRHFVEVGRGSGLPDRQFAPEAMQWLERHDWPGNVRELGN
EEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHH
VVQRLAVLSRDTVVTAREVEMVLRDNDESGGVADPAALIARAVDDWAREKMASAEPDGGI
HHHHHHHHHCCCEEHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
HGQLEAIVEAALFRRVLRDVRGNQLEAARRLGINRNTLRKRLAQLEIDPLHP
CHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SEGKTILLVEDDPAIAMVIRETLDGECGLLASVGSIAERNAWLAAHRADLIITDVVLPD
CCCCEEEEEECCCHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHCCCEEEEEEEECC
GDGIDSLVHAGVDPATPVIVLSAQNTLDTAVRATGIGSYDYLPKPFDLDELTASVRAALQ
CCCHHHHHHCCCCCCCCEEEEECCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHH
RRVEAAASDAPPPADSHGLVGRAPAMQAVYRTIARLATNDLAVLILGESGTGKEVVARAI
HHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHH
HATGLRRDGPFVAINMAAIPRELIEAELFGHEKGAFTGAHSRNAGRFEQAAGGTLFLDEI
HHHCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCEEEHHHH
GDMPLEAQTRLLRVLQSNEYSTVGGSQALRADVRVIAATHRDMRTLVADGRFREDLFYRL
CCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHEEE
NVIPVTLPPLRDRRSDIAALVRHFVEVGRGSGLPDRQFAPEAMQWLERHDWPGNVRELGN
EEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHH
VVQRLAVLSRDTVVTAREVEMVLRDNDESGGVADPAALIARAVDDWAREKMASAEPDGGI
HHHHHHHHHCCCEEHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
HGQLEAIVEAALFRRVLRDVRGNQLEAARRLGINRNTLRKRLAQLEIDPLHP
CHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7553451 [H]