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The map label for this gene is ntrB [H]

Identifier: 103486762

GI number: 103486762

Start: 1333835

End: 1334926

Strand: Reverse

Name: ntrB [H]

Synonym: Sala_1276

Alternate gene names: 103486762

Gene position: 1334926-1333835 (Counterclockwise)

Preceding gene: 103486763

Following gene: 103486761

Centisome position: 39.91

GC content: 65.66

Gene sequence:

>1092_bases
GTGACCACGCATGCGTCGAGCATCCCGAGCTTCGACCATGACGAGCTGATCCAGTCGCACCCGGTGCCGACGTTATTGAT
CGACAGCGGTGGGGTGGTCCTGTTCGTCAACGCCGCGGCCGAACAGCTTTGTAACGTCAGCCGGTCGGCGATGGTCGGGC
GCGTTATCTATGACGTCATCCAGATGGATCGCAGTTATCGCCAGCGGATGAACGACGCCGCGACGTCGGCGCTCTTTGCG
CATCGCGCCGAAATCACGCTTGGCGGCCGCAAATCGGTCTTTGTCGACATGCAGATGGTGCCTTATGGTCAGAGCGGCCA
TCGCATCCTCGCGCTCATTCCTTCGCAAAGCGACACCGAACTGATGGGCGGCGCGATCGGGCGGTCGAGCCGCGCCGCGG
GGGCGGCGGCGTCGATGCTGGCGCACGAGATCAAGAATCCGCTTGCCGGGATCAAGGGCGCGGCGCAGTTGCTCGCGCGC
AAGACCGGTGCCGGCGGGGAACGCTTCACGACGCTGATCTGCGCCGAGGTCGACCGCATCGCGACGCTGATCGACCAGAT
GGAGCATTTCTCGCGCGGGCAACCGATCCTCTGCGGGCCGATCAACCTCTATCAGCCGATCCATCAGGCGATGGAAACCG
CGCGCGCGCGCCAGTTTCCGGGTGTCCGCTTCGCCGAGGATTTCGACCCGTCGCTGCCGCTCGTCCACGGCAACCACGAC
GCCATGGTGCAGATATTGCTCAACCTCGTGACCAACGGGTGCGAAGCGCTGGCGGGACGCGACGATGGCGTGGTGCGGAT
CGCGACCGCCTATCGGCACGGCCTGTCGATCGACAATGGCGACGGGCGCGGACGTATCGCGCTGCCGATCGAGGTCAGTG
TCAGCGACAATGGCCCCGGCGTACCCAGGGACATCCGCGGCGACCTGTTCGATCCGTTCGTGACGACGAAGCGCGAGGGG
AGGGGACTTGGCCTTGCGCTCGTCGCCAAGCTGGCGCGCGACATGGGCGGGACGGTGCAGCATATGCGCGATGGCGACTG
GACACGCTTTCGCGTCCACCTGCCGGTCGCGCAAGCAAAGGGACGGGGATGA

Upstream 100 bases:

>100_bases
CGGCCGAGTTCCGCAACAAGGTCAACCGGATTCCCGACAGCAAGGGCGTGCTCGACGCGCTCGAGGCCTTTTATGGCCCG
CATCTGACCCGGGCCGCCGC

Downstream 100 bases:

>100_bases
GCGAAGGCAAGACGATCCTGCTGGTCGAGGACGATCCGGCGATCGCGATGGTCATCCGCGAAACGCTCGACGGCGAATGC
GGCCTTCTCGCGTCGGTCGG

Product: signal transduction histidine kinase, nitrogen specific, NtrB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 363; Mature: 362

Protein sequence:

>363_residues
MTTHASSIPSFDHDELIQSHPVPTLLIDSGGVVLFVNAAAEQLCNVSRSAMVGRVIYDVIQMDRSYRQRMNDAATSALFA
HRAEITLGGRKSVFVDMQMVPYGQSGHRILALIPSQSDTELMGGAIGRSSRAAGAAASMLAHEIKNPLAGIKGAAQLLAR
KTGAGGERFTTLICAEVDRIATLIDQMEHFSRGQPILCGPINLYQPIHQAMETARARQFPGVRFAEDFDPSLPLVHGNHD
AMVQILLNLVTNGCEALAGRDDGVVRIATAYRHGLSIDNGDGRGRIALPIEVSVSDNGPGVPRDIRGDLFDPFVTTKREG
RGLGLALVAKLARDMGGTVQHMRDGDWTRFRVHLPVAQAKGRG

Sequences:

>Translated_363_residues
MTTHASSIPSFDHDELIQSHPVPTLLIDSGGVVLFVNAAAEQLCNVSRSAMVGRVIYDVIQMDRSYRQRMNDAATSALFA
HRAEITLGGRKSVFVDMQMVPYGQSGHRILALIPSQSDTELMGGAIGRSSRAAGAAASMLAHEIKNPLAGIKGAAQLLAR
KTGAGGERFTTLICAEVDRIATLIDQMEHFSRGQPILCGPINLYQPIHQAMETARARQFPGVRFAEDFDPSLPLVHGNHD
AMVQILLNLVTNGCEALAGRDDGVVRIATAYRHGLSIDNGDGRGRIALPIEVSVSDNGPGVPRDIRGDLFDPFVTTKREG
RGLGLALVAKLARDMGGTVQHMRDGDWTRFRVHLPVAQAKGRG
>Mature_362_residues
TTHASSIPSFDHDELIQSHPVPTLLIDSGGVVLFVNAAAEQLCNVSRSAMVGRVIYDVIQMDRSYRQRMNDAATSALFAH
RAEITLGGRKSVFVDMQMVPYGQSGHRILALIPSQSDTELMGGAIGRSSRAAGAAASMLAHEIKNPLAGIKGAAQLLARK
TGAGGERFTTLICAEVDRIATLIDQMEHFSRGQPILCGPINLYQPIHQAMETARARQFPGVRFAEDFDPSLPLVHGNHDA
MVQILLNLVTNGCEALAGRDDGVVRIATAYRHGLSIDNGDGRGRIALPIEVSVSDNGPGVPRDIRGDLFDPFVTTKREGR
GLGLALVAKLARDMGGTVQHMRDGDWTRFRVHLPVAQAKGRG

Specific function: NtrB acts as a signal transducer which responds to the nitrogen level of cell and modulates the activity of ntrC. In nitrogen limitation ntrB activates ntrC by phosphorylating it, while in nitrogen excess ntrC is dephosphorylated and consequently inactiva

COG id: COG3852

COG function: function code T; Signal transduction histidine kinase, nitrogen specific

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790300, Length=347, Percent_Identity=30.2593659942363, Blast_Score=121, Evalue=7e-29,
Organism=Escherichia coli, GI1790436, Length=232, Percent_Identity=30.1724137931034, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1788549, Length=334, Percent_Identity=26.0479041916168, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI48994928, Length=293, Percent_Identity=25.2559726962457, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 39069; Mature: 38938

Theoretical pI: Translated: 8.06; Mature: 8.06

Prosite motif: PS50112 PAS ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTHASSIPSFDHDELIQSHPVPTLLIDSGGVVLFVNAAAEQLCNVSRSAMVGRVIYDVI
CCCCCCCCCCCCHHHHHHHCCCCEEEECCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHH
QMDRSYRQRMNDAATSALFAHRAEITLGGRKSVFVDMQMVPYGQSGHRILALIPSQSDTE
HHHHHHHHHHHHHHHHHHHHHHEEEEECCCCEEEEEEEEECCCCCCCEEEEEECCCCCCH
LMGGAIGRSSRAAGAAASMLAHEIKNPLAGIKGAAQLLARKTGAGGERFTTLICAEVDRI
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
ATLIDQMEHFSRGQPILCGPINLYQPIHQAMETARARQFPGVRFAEDFDPSLPLVHGNHD
HHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCHH
AMVQILLNLVTNGCEALAGRDDGVVRIATAYRHGLSIDNGDGRGRIALPIEVSVSDNGPG
HHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHCCCCCCCCCCCCEEEEEEEEEECCCCCC
VPRDIRGDLFDPFVTTKREGRGLGLALVAKLARDMGGTVQHMRDGDWTRFRVHLPVAQAK
CCCCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHCCCHHHHHCCCCCEEEEEEEEHHHCC
GRG
CCC
>Mature Secondary Structure 
TTHASSIPSFDHDELIQSHPVPTLLIDSGGVVLFVNAAAEQLCNVSRSAMVGRVIYDVI
CCCCCCCCCCCHHHHHHHCCCCEEEECCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHH
QMDRSYRQRMNDAATSALFAHRAEITLGGRKSVFVDMQMVPYGQSGHRILALIPSQSDTE
HHHHHHHHHHHHHHHHHHHHHHEEEEECCCCEEEEEEEEECCCCCCCEEEEEECCCCCCH
LMGGAIGRSSRAAGAAASMLAHEIKNPLAGIKGAAQLLARKTGAGGERFTTLICAEVDRI
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
ATLIDQMEHFSRGQPILCGPINLYQPIHQAMETARARQFPGVRFAEDFDPSLPLVHGNHD
HHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCHH
AMVQILLNLVTNGCEALAGRDDGVVRIATAYRHGLSIDNGDGRGRIALPIEVSVSDNGPG
HHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHCCCCCCCCCCCCEEEEEEEEEECCCCCC
VPRDIRGDLFDPFVTTKREGRGLGLALVAKLARDMGGTVQHMRDGDWTRFRVHLPVAQAK
CCCCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHCCCHHHHHCCCCCEEEEEEEEHHHCC
GRG
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3020561 [H]