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The map label for this gene is dacA [H]

Identifier: 103486742

GI number: 103486742

Start: 1308924

End: 1309745

Strand: Direct

Name: dacA [H]

Synonym: Sala_1256

Alternate gene names: 103486742

Gene position: 1308924-1309745 (Clockwise)

Preceding gene: 103486741

Following gene: 103486743

Centisome position: 39.13

GC content: 61.31

Gene sequence:

>822_bases
ATGCTCAAGGACATGGAGTCGGGCGCGATCCTTTTTTCGCGGGGTGCCGACAAACGCTTCGCCCCGGCTTCGATGGCGAA
GGTGATGACCGCCTATGTCGTGCTTGATCTTTTAAAAACGCGCGAACTGCGGCGCGACACGACATTCACGGTCAGCGACG
CCGTATGGAGGAAATGGAACAGCAGTACCGGTGGGTCGACGATGTTCCTCAGTCCCGGCGAGAAACTGTCGGTTGACGAT
CTGCTGAAGGGTTTGATCACCGTATCGGCGAATGATGCGGCGGCGGTTCTTGCCGAAGGAATCGATGGCAGCGAAGAAGC
TTTTGTCAGGCGAATGAATAAGGTGGCGGCTGATATTGGTATGGCGTCGAGTCATTTTGGCACGCCCAACGGCTGGCCGG
ACGGCGGCGTCACAAAGGTCAGCGCCGCTGACCTCATCACGCTTGCCGATCGACTTATCCGTGATCATCCGGACGGTTAT
GCGCGTTATTTTTCAATCCCGAAACTCCAGCATGGCGTCGCGCCCGACGGACGACCGATCGTGCAACCGAACCGCAACCC
GATTCTCGACCGGGTTGACGGCGCCGATGGCCTCAAAACGGGGCATACCCGCGAGGCAGGCTATTGCTTCCTGGGGTCGG
CCGAACGCGATGGCCGCCGGCTGATCATGGTCGTTGCCGGTCTGCCCAGCGCAAATGCGCGGCGGGAGGAGGCGCAAAGG
CTGATGGACTGGGGGTTCGCCGCCGCAGCGCATCGAATGGCAGCCGAAACCCGGCGTGGGAGCGCCTCAACGAGCGTGGC
CGCGGGCGCGACGCATAGATGA

Upstream 100 bases:

>100_bases
TTTTTGGCCCGCTGCAGCGGCGAGGGATAATCGCTCATCCGGCGCCGCCAGCGCCGCGGCGGTCAGTCCGGCGCCCTATG
CGACACAGGCGCCGATCGTC

Downstream 100 bases:

>100_bases
CAAAGCCGGCGGGGCAGTTTACCTCCCGTGCCATGTCTCGACACCAGCCCATCCTCCCGTCGTCCGGTCTGACGCCGTGA
TCCGCGGACGCTTCATCACG

Product: Serine-type D-Ala-D-Ala carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5; PBP-5 [H]

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MLKDMESGAILFSRGADKRFAPASMAKVMTAYVVLDLLKTRELRRDTTFTVSDAVWRKWNSSTGGSTMFLSPGEKLSVDD
LLKGLITVSANDAAAVLAEGIDGSEEAFVRRMNKVAADIGMASSHFGTPNGWPDGGVTKVSAADLITLADRLIRDHPDGY
ARYFSIPKLQHGVAPDGRPIVQPNRNPILDRVDGADGLKTGHTREAGYCFLGSAERDGRRLIMVVAGLPSANARREEAQR
LMDWGFAAAAHRMAAETRRGSASTSVAAGATHR

Sequences:

>Translated_273_residues
MLKDMESGAILFSRGADKRFAPASMAKVMTAYVVLDLLKTRELRRDTTFTVSDAVWRKWNSSTGGSTMFLSPGEKLSVDD
LLKGLITVSANDAAAVLAEGIDGSEEAFVRRMNKVAADIGMASSHFGTPNGWPDGGVTKVSAADLITLADRLIRDHPDGY
ARYFSIPKLQHGVAPDGRPIVQPNRNPILDRVDGADGLKTGHTREAGYCFLGSAERDGRRLIMVVAGLPSANARREEAQR
LMDWGFAAAAHRMAAETRRGSASTSVAAGATHR
>Mature_273_residues
MLKDMESGAILFSRGADKRFAPASMAKVMTAYVVLDLLKTRELRRDTTFTVSDAVWRKWNSSTGGSTMFLSPGEKLSVDD
LLKGLITVSANDAAAVLAEGIDGSEEAFVRRMNKVAADIGMASSHFGTPNGWPDGGVTKVSAADLITLADRLIRDHPDGY
ARYFSIPKLQHGVAPDGRPIVQPNRNPILDRVDGADGLKTGHTREAGYCFLGSAERDGRRLIMVVAGLPSANARREEAQR
LMDWGFAAAAHRMAAETRRGSASTSVAAGATHR

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein. Note=N-terminal lies in the periplasmic space (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=252, Percent_Identity=37.3015873015873, Blast_Score=144, Evalue=5e-36,
Organism=Escherichia coli, GI87082022, Length=247, Percent_Identity=38.8663967611336, Blast_Score=143, Evalue=1e-35,
Organism=Escherichia coli, GI1786851, Length=249, Percent_Identity=36.144578313253, Blast_Score=138, Evalue=4e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 29331; Mature: 29331

Theoretical pI: Translated: 9.74; Mature: 9.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKDMESGAILFSRGADKRFAPASMAKVMTAYVVLDLLKTRELRRDTTFTVSDAVWRKWN
CCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHCC
SSTGGSTMFLSPGEKLSVDDLLKGLITVSANDAAAVLAEGIDGSEEAFVRRMNKVAADIG
CCCCCCEEEECCCCCCCHHHHHHHHEEECCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHC
MASSHFGTPNGWPDGGVTKVSAADLITLADRLIRDHPDGYARYFSIPKLQHGVAPDGRPI
CCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHCCCCHHHHCCCCHHHCCCCCCCCCC
VQPNRNPILDRVDGADGLKTGHTREAGYCFLGSAERDGRRLIMVVAGLPSANARREEAQR
CCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEEEEECCCCCCHHHHHHHH
LMDWGFAAAAHRMAAETRRGSASTSVAAGATHR
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLKDMESGAILFSRGADKRFAPASMAKVMTAYVVLDLLKTRELRRDTTFTVSDAVWRKWN
CCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHCC
SSTGGSTMFLSPGEKLSVDDLLKGLITVSANDAAAVLAEGIDGSEEAFVRRMNKVAADIG
CCCCCCEEEECCCCCCCHHHHHHHHEEECCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHC
MASSHFGTPNGWPDGGVTKVSAADLITLADRLIRDHPDGYARYFSIPKLQHGVAPDGRPI
CCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHCCCCHHHHCCCCHHHCCCCCCCCCC
VQPNRNPILDRVDGADGLKTGHTREAGYCFLGSAERDGRRLIMVVAGLPSANARREEAQR
CCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEEEEECCCCCCHHHHHHHH
LMDWGFAAAAHRMAAETRRGSASTSVAAGATHR
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]