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The map label for this gene is puuA [H]

Identifier: 103486606

GI number: 103486606

Start: 1153670

End: 1155061

Strand: Reverse

Name: puuA [H]

Synonym: Sala_1118

Alternate gene names: 103486606

Gene position: 1155061-1153670 (Counterclockwise)

Preceding gene: 103486607

Following gene: 103486605

Centisome position: 34.53

GC content: 65.37

Gene sequence:

>1392_bases
ATGTCGAAGTCATCGGGCGTGATTGCGGACCGGTCCGAAGCCGAGGCCTTTTTCAAGGCGAACCCTGACGTCGATGCGAT
CGAGATGATCTATACCGACATGGGCGGCGTCCCGCGCGGCAAGCGGCTGCGCCAGCATGAAGTGATGGCCGTCTACGACA
GCGGGCGCATGTTCCCCGGTTCGATCACCGTCGTCGACATCACCGGACAGGATACCGTCGAAACCGGACTCGTCTGGGAA
GATGGTGATGCCGACCGCTCGATGAAGCCGATCCCCGGCACCCTCGTTCGCACGCCCTGGGGAGGCGACGGCGCGGCACA
GTTCCTTGTCGATTTCTATGAGCTCGACGGGACGCCGCACGACCTCGACCCGCGCCATGTGCTCGGCCGCGTGATCGACC
GCTTTACCGCCGACGCGCTCACCCCGGTGCTCGCGGTCGAGCTGGAGTTTTACCTTGTCGATCCGCGCCGCGCACGCGAC
GGCGGCATCCGCCCGGCCCGCCCCGGCTACAGCCGCAGCGCGCCGCGCAATGTCGAGGTATACGGCCTGCGCGAACTGGA
GGATTTCCGGCCTTTTTTTGACGCACTCTACACGGCGACCGACGTGCAGGGCCTGCCGCTCGAAAGCGCGATCAGCGAAT
TCGCTCCCGGCCAGTTCGAGCTGACCTTGCGCCACAAGCCCGACGCGCTGCGCGCCGCCGACGATGCGATCATGTACAAA
AGGCTGGTCAAGGCGATTGCCCAGCAACACGGCCTCGAAGCCACCTTCATGGCGAAACCCTTTGCCGATCAGGCGGGCAG
CGGGATGCACATCCATGTTTCGGTGAACGACGCAGGCGGCACCAATATCTTCGCAAGCGAAGACCCCGAGGGAACCGACG
CGCTGCGCCACGCGATCGGCGGGATGATCGGCAGCGTCGGCGACGCCTTCGCCATCTTTGCCCCCCACGCGAACAGCTAT
CGCCGCTTCAAGGCGAACAGCTATGCTCCCGTCGCGCCGACCTGGGGCGTCAACAACCGCACCGTGTCGTTCCGCATTCC
CGCCGGCCCGCCGCCGAGCCGCCATGTCGAACATCGCGCGTGCGGCGCCGACGCCAACCCCTATCTCGCGGTCGCGGCGG
TGCTTGCCGGGATGCATCACGGCATGACGACGAGGACCGACCCCGGCGCCGCGGTGGTCGGCAACGGTTATGACCGCGAC
AACAGCGGCGACGACAAGCCGCCGTCGAACTGGTTCGCCGCGGTCGACCGTTTCCACGATTCGGCGCTCATGCGCGACTA
TCTGGGCGACCGGTTCGTCGACATGTTCAGCATCGTCAAGCGCGTCGAACAGGACAATTATTTCAGCGTCGTGCCGGCGC
TCGATTATGATTGGTACCTACGCAACGCATGA

Upstream 100 bases:

>100_bases
AATATGATGTTGGTGAGTCCGGTGACGGGCGACGATCAGATCGACCGGCTGGTCGGCGCGTTCGCCGACTGCGTGAGCGC
GTTGAAGGAGTAAGCGGGCG

Downstream 100 bases:

>100_bases
AAGTTTCATATTATCCTGGTGCAGCGCAAAAAAGCCTCTTTCCAAGCCGCGTTTAATGAGTCAGCTTGGCGCTTCACACA
GGGAGGCTGGCCATGGACTT

Product: glutamate--ammonia ligase

Products: NA

Alternate protein names: Gamma-Glu-Put synthetase; Glutamate--putrescine ligase [H]

Number of amino acids: Translated: 463; Mature: 462

Protein sequence:

>463_residues
MSKSSGVIADRSEAEAFFKANPDVDAIEMIYTDMGGVPRGKRLRQHEVMAVYDSGRMFPGSITVVDITGQDTVETGLVWE
DGDADRSMKPIPGTLVRTPWGGDGAAQFLVDFYELDGTPHDLDPRHVLGRVIDRFTADALTPVLAVELEFYLVDPRRARD
GGIRPARPGYSRSAPRNVEVYGLRELEDFRPFFDALYTATDVQGLPLESAISEFAPGQFELTLRHKPDALRAADDAIMYK
RLVKAIAQQHGLEATFMAKPFADQAGSGMHIHVSVNDAGGTNIFASEDPEGTDALRHAIGGMIGSVGDAFAIFAPHANSY
RRFKANSYAPVAPTWGVNNRTVSFRIPAGPPPSRHVEHRACGADANPYLAVAAVLAGMHHGMTTRTDPGAAVVGNGYDRD
NSGDDKPPSNWFAAVDRFHDSALMRDYLGDRFVDMFSIVKRVEQDNYFSVVPALDYDWYLRNA

Sequences:

>Translated_463_residues
MSKSSGVIADRSEAEAFFKANPDVDAIEMIYTDMGGVPRGKRLRQHEVMAVYDSGRMFPGSITVVDITGQDTVETGLVWE
DGDADRSMKPIPGTLVRTPWGGDGAAQFLVDFYELDGTPHDLDPRHVLGRVIDRFTADALTPVLAVELEFYLVDPRRARD
GGIRPARPGYSRSAPRNVEVYGLRELEDFRPFFDALYTATDVQGLPLESAISEFAPGQFELTLRHKPDALRAADDAIMYK
RLVKAIAQQHGLEATFMAKPFADQAGSGMHIHVSVNDAGGTNIFASEDPEGTDALRHAIGGMIGSVGDAFAIFAPHANSY
RRFKANSYAPVAPTWGVNNRTVSFRIPAGPPPSRHVEHRACGADANPYLAVAAVLAGMHHGMTTRTDPGAAVVGNGYDRD
NSGDDKPPSNWFAAVDRFHDSALMRDYLGDRFVDMFSIVKRVEQDNYFSVVPALDYDWYLRNA
>Mature_462_residues
SKSSGVIADRSEAEAFFKANPDVDAIEMIYTDMGGVPRGKRLRQHEVMAVYDSGRMFPGSITVVDITGQDTVETGLVWED
GDADRSMKPIPGTLVRTPWGGDGAAQFLVDFYELDGTPHDLDPRHVLGRVIDRFTADALTPVLAVELEFYLVDPRRARDG
GIRPARPGYSRSAPRNVEVYGLRELEDFRPFFDALYTATDVQGLPLESAISEFAPGQFELTLRHKPDALRAADDAIMYKR
LVKAIAQQHGLEATFMAKPFADQAGSGMHIHVSVNDAGGTNIFASEDPEGTDALRHAIGGMIGSVGDAFAIFAPHANSYR
RFKANSYAPVAPTWGVNNRTVSFRIPAGPPPSRHVEHRACGADANPYLAVAAVLAGMHHGMTTRTDPGAAVVGNGYDRDN
SGDDKPPSNWFAAVDRFHDSALMRDYLGDRFVDMFSIVKRVEQDNYFSVVPALDYDWYLRNA

Specific function: Involved in the breakdown of putrescine via the biosynthesis of gamma-L-glutamylputrescine [H]

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Escherichia coli, GI87081870, Length=454, Percent_Identity=35.6828193832599, Blast_Score=303, Evalue=1e-83,
Organism=Escherichia coli, GI1790301, Length=479, Percent_Identity=27.348643006263, Blast_Score=121, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146 [H]

Pfam domain/function: PF00120 Gln-synt_C [H]

EC number: =6.3.1.11 [H]

Molecular weight: Translated: 50727; Mature: 50596

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKSSGVIADRSEAEAFFKANPDVDAIEMIYTDMGGVPRGKRLRQHEVMAVYDSGRMFPG
CCCCCCCEECCHHHHHEEECCCCCHHHHHHHHHCCCCCCCCHHCCCCEEEEEECCCCCCC
SITVVDITGQDTVETGLVWEDGDADRSMKPIPGTLVRTPWGGDGAAQFLVDFYELDGTPH
EEEEEEECCCCCCCCCCEECCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHCCCCCC
DLDPRHVLGRVIDRFTADALTPVLAVELEFYLVDPRRARDGGIRPARPGYSRSAPRNVEV
CCCHHHHHHHHHHHHHHHHHCCEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEE
YGLRELEDFRPFFDALYTATDVQGLPLESAISEFAPGQFELTLRHKPDALRAADDAIMYK
EECHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHH
RLVKAIAQQHGLEATFMAKPFADQAGSGMHIHVSVNDAGGTNIFASEDPEGTDALRHAIG
HHHHHHHHHHCCCEEEEECCCHHHCCCCEEEEEEEECCCCCEEECCCCCCHHHHHHHHHH
GMIGSVGDAFAIFAPHANSYRRFKANSYAPVAPTWGVNNRTVSFRIPAGPPPSRHVEHRA
HHHHCCCCEEEEECCCCCCCCEECCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCHHH
CGADANPYLAVAAVLAGMHHGMTTRTDPGAAVVGNGYDRDNSGDDKPPSNWFAAVDRFHD
CCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHH
SALMRDYLGDRFVDMFSIVKRVEQDNYFSVVPALDYDWYLRNA
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEECCC
>Mature Secondary Structure 
SKSSGVIADRSEAEAFFKANPDVDAIEMIYTDMGGVPRGKRLRQHEVMAVYDSGRMFPG
CCCCCCEECCHHHHHEEECCCCCHHHHHHHHHCCCCCCCCHHCCCCEEEEEECCCCCCC
SITVVDITGQDTVETGLVWEDGDADRSMKPIPGTLVRTPWGGDGAAQFLVDFYELDGTPH
EEEEEEECCCCCCCCCCEECCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHCCCCCC
DLDPRHVLGRVIDRFTADALTPVLAVELEFYLVDPRRARDGGIRPARPGYSRSAPRNVEV
CCCHHHHHHHHHHHHHHHHHCCEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEE
YGLRELEDFRPFFDALYTATDVQGLPLESAISEFAPGQFELTLRHKPDALRAADDAIMYK
EECHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHH
RLVKAIAQQHGLEATFMAKPFADQAGSGMHIHVSVNDAGGTNIFASEDPEGTDALRHAIG
HHHHHHHHHHCCCEEEEECCCHHHCCCCEEEEEEEECCCCCEEECCCCCCHHHHHHHHHH
GMIGSVGDAFAIFAPHANSYRRFKANSYAPVAPTWGVNNRTVSFRIPAGPPPSRHVEHRA
HHHHCCCCEEEEECCCCCCCCEECCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCHHH
CGADANPYLAVAAVLAGMHHGMTTRTDPGAAVVGNGYDRDNSGDDKPPSNWFAAVDRFHD
CCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHH
SALMRDYLGDRFVDMFSIVKRVEQDNYFSVVPALDYDWYLRNA
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]