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The map label for this gene is potF [H]

Identifier: 103486605

GI number: 103486605

Start: 1152471

End: 1153577

Strand: Reverse

Name: potF [H]

Synonym: Sala_1117

Alternate gene names: 103486605

Gene position: 1153577-1152471 (Counterclockwise)

Preceding gene: 103486606

Following gene: 103486604

Centisome position: 34.48

GC content: 59.08

Gene sequence:

>1107_bases
ATGGACTTCAACGAACTGATCAAGCAGACCCGCGGGCGCCGCTCGCTCCTTCAGGCGCTTGGTGTCGCCGCCGTCGGGAT
CAGTTTCGGCGGGCTCGCCGCGTGCAGCAAGGAAGGCGGCAAGACGCTGGCGAATGGCGAAGAGGCCAGGCTCAACTTCT
ATAACTGGGACACTTATATCGGCGAAACGACGCTGGCCGATTTCGAGGAAGCGACGGGCGTCGCGGTGACGATGGACCTG
TTCGACAGCAACGACGTGCTGTTCGCCAAGTTCAAGGCCGGCAATCCCGGCTATGACGTGATCGTGCCGTCGAACGACTT
CGTCGAACGGATGAGCAAGGCCGACATGCTGATGCCGCTCGACCACAGCCTCATTCCGAACAAGAAAAATATCGACGCCG
CCTATATCAACGTCGAATATGACCTGCAGCGCAAGTTTTCGATGCCCTACACCTGGCTCGCGCTCGGCATCGGCTATCGC
AAGTCGAAGGTGTCAGCGACGCCCGACAGCTGGAAAGTGCTGTTCGACAGCCCCGAATATGCCGGCCGCATCGCCTGGCT
GTCGGAGGCGGGCGACATGTTCCGCCTGTTCGGCAAATATCTCGGAAAATCGGTCAACGAACTGACCCCCGCCGACGTCG
CGACGATCGAACGGATGATGATCAAGCAAAAGCCCAATGTGAAGAAGTTCCACGAGGATGACGGGCAGGATCTGCTGCTC
AAGGGAGATGTCGACGTCGTGCTCGAATATAATGGCGACATCGCACAGGCGATGGTCGAGGATGACGATATCGACTTCGT
CATCCCCAAGGAAGGCAGCCAGCTCAATTCGGACAATCTGTGCATCCCGAAGGGCGCGCCGCATCCGAAGAACGCCCATG
CCTTTATCAACTATATCCTCGACGCGAATGTCGACAAGCATATCACCGAGACGATCCTCTATCCGACGCCCAACGCCGCG
GCGAAGGCGCTGATGCCCGAAAGCTACAAGAACAACCCGGTGATCTTCCCGCCCGCCGATGTGCTCGCCAAGTGCGAATA
TGCACGCTTCAACCCCGAGCTTCAGCCACTCTTCGAAGAGGCGTTCACGCGCGTGCGGGCGGCCTGA

Upstream 100 bases:

>100_bases
ACGCATGAAAGTTTCATATTATCCTGGTGCAGCGCAAAAAAGCCTCTTTCCAAGCCGCGTTTAATGAGTCAGCTTGGCGC
TTCACACAGGGAGGCTGGCC

Downstream 100 bases:

>100_bases
CGCGATCCAGGGACGGCGGGGGTGGCCGAGCAGGACTGGAAAACCAACAGGGGCGTTTTCGCGGCGGTGTCGCTGCCGAC
GCTCCTGTGGGTTCTGCTCT

Product: extracellular solute-binding protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MDFNELIKQTRGRRSLLQALGVAAVGISFGGLAACSKEGGKTLANGEEARLNFYNWDTYIGETTLADFEEATGVAVTMDL
FDSNDVLFAKFKAGNPGYDVIVPSNDFVERMSKADMLMPLDHSLIPNKKNIDAAYINVEYDLQRKFSMPYTWLALGIGYR
KSKVSATPDSWKVLFDSPEYAGRIAWLSEAGDMFRLFGKYLGKSVNELTPADVATIERMMIKQKPNVKKFHEDDGQDLLL
KGDVDVVLEYNGDIAQAMVEDDDIDFVIPKEGSQLNSDNLCIPKGAPHPKNAHAFINYILDANVDKHITETILYPTPNAA
AKALMPESYKNNPVIFPPADVLAKCEYARFNPELQPLFEEAFTRVRAA

Sequences:

>Translated_368_residues
MDFNELIKQTRGRRSLLQALGVAAVGISFGGLAACSKEGGKTLANGEEARLNFYNWDTYIGETTLADFEEATGVAVTMDL
FDSNDVLFAKFKAGNPGYDVIVPSNDFVERMSKADMLMPLDHSLIPNKKNIDAAYINVEYDLQRKFSMPYTWLALGIGYR
KSKVSATPDSWKVLFDSPEYAGRIAWLSEAGDMFRLFGKYLGKSVNELTPADVATIERMMIKQKPNVKKFHEDDGQDLLL
KGDVDVVLEYNGDIAQAMVEDDDIDFVIPKEGSQLNSDNLCIPKGAPHPKNAHAFINYILDANVDKHITETILYPTPNAA
AKALMPESYKNNPVIFPPADVLAKCEYARFNPELQPLFEEAFTRVRAA
>Mature_368_residues
MDFNELIKQTRGRRSLLQALGVAAVGISFGGLAACSKEGGKTLANGEEARLNFYNWDTYIGETTLADFEEATGVAVTMDL
FDSNDVLFAKFKAGNPGYDVIVPSNDFVERMSKADMLMPLDHSLIPNKKNIDAAYINVEYDLQRKFSMPYTWLALGIGYR
KSKVSATPDSWKVLFDSPEYAGRIAWLSEAGDMFRLFGKYLGKSVNELTPADVATIERMMIKQKPNVKKFHEDDGQDLLL
KGDVDVVLEYNGDIAQAMVEDDDIDFVIPKEGSQLNSDNLCIPKGAPHPKNAHAFINYILDANVDKHITETILYPTPNAA
AKALMPESYKNNPVIFPPADVLAKCEYARFNPELQPLFEEAFTRVRAA

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787078, Length=365, Percent_Identity=35.0684931506849, Blast_Score=197, Evalue=1e-51,
Organism=Escherichia coli, GI1787367, Length=320, Percent_Identity=31.875, Blast_Score=174, Evalue=7e-45,
Organism=Escherichia coli, GI1787711, Length=314, Percent_Identity=23.5668789808917, Blast_Score=72, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 40930; Mature: 40930

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFNELIKQTRGRRSLLQALGVAAVGISFGGLAACSKEGGKTLANGEEARLNFYNWDTYI
CCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCEEEEEECCHHC
GETTLADFEEATGVAVTMDLFDSNDVLFAKFKAGNPGYDVIVPSNDFVERMSKADMLMPL
CCCHHHHHHHCCCEEEEEEEECCCCEEEEEEECCCCCEEEEECCHHHHHHHHHCCCCCCC
DHSLIPNKKNIDAAYINVEYDLQRKFSMPYTWLALGIGYRKSKVSATPDSWKVLFDSPEY
CCCCCCCCCCCCEEEEEEEEEHHHHCCCCEEEEHEECCCCCCCCCCCCCCCEEEECCCCC
AGRIAWLSEAGDMFRLFGKYLGKSVNELTPADVATIERMMIKQKPNVKKFHEDDGQDLLL
CCEEEEEHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEE
KGDVDVVLEYNGDIAQAMVEDDDIDFVIPKEGSQLNSDNLCIPKGAPHPKNAHAFINYIL
ECCEEEEEEECCCHHHHHCCCCCCCEEECCCCCCCCCCCEECCCCCCCCCCHHHHEEEEC
DANVDKHITETILYPTPNAAAKALMPESYKNNPVIFPPADVLAKCEYARFNPELQPLFEE
CCCCCHHHHHEEEECCCCHHHHHHCCCCCCCCCEEECCHHHHHHCHHHHCCCCHHHHHHH
AFTRVRAA
HHHHHCCC
>Mature Secondary Structure
MDFNELIKQTRGRRSLLQALGVAAVGISFGGLAACSKEGGKTLANGEEARLNFYNWDTYI
CCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCEEEEEECCHHC
GETTLADFEEATGVAVTMDLFDSNDVLFAKFKAGNPGYDVIVPSNDFVERMSKADMLMPL
CCCHHHHHHHCCCEEEEEEEECCCCEEEEEEECCCCCEEEEECCHHHHHHHHHCCCCCCC
DHSLIPNKKNIDAAYINVEYDLQRKFSMPYTWLALGIGYRKSKVSATPDSWKVLFDSPEY
CCCCCCCCCCCCEEEEEEEEEHHHHCCCCEEEEHEECCCCCCCCCCCCCCCEEEECCCCC
AGRIAWLSEAGDMFRLFGKYLGKSVNELTPADVATIERMMIKQKPNVKKFHEDDGQDLLL
CCEEEEEHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEE
KGDVDVVLEYNGDIAQAMVEDDDIDFVIPKEGSQLNSDNLCIPKGAPHPKNAHAFINYIL
ECCEEEEEEECCCHHHHHCCCCCCCEEECCCCCCCCCCCEECCCCCCCCCCHHHHEEEEC
DANVDKHITETILYPTPNAAAKALMPESYKNNPVIFPPADVLAKCEYARFNPELQPLFEE
CCCCCHHHHHEEEECCCCHHHHHHCCCCCCCCCEEECCHHHHHHCHHHHCCCCHHHHHHH
AFTRVRAA
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: Polyamine Binding Protein [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503; 9298646; 9651355 [H]