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The map label for this gene is hemL [H]

Identifier: 103486607

GI number: 103486607

Start: 1155069

End: 1156412

Strand: Reverse

Name: hemL [H]

Synonym: Sala_1119

Alternate gene names: 103486607

Gene position: 1156412-1155069 (Counterclockwise)

Preceding gene: 103486608

Following gene: 103486606

Centisome position: 34.57

GC content: 67.56

Gene sequence:

>1344_bases
ATGATCGCCGGGGCCGCGATCGCGCGCGTCGCGGCGCGCGAGGCGGAGCGCTTTCGCACCCTCAATCCGCGCGCTTTCGC
GCATCATGCGGCAGCAAAAGGCTGGTTCCAGGCAGTCCCCTTCCACTGGATGACCGACTGGCCGAGCCCGGTGCCCATCG
TCGCTGCATCGGCGCGCGGCGCGACGCTGACCAGCATCGACGGCCAGCGTTACGACGATTTCTGCCTGGGCGACACGGCG
AGCCTGTTCGGTCATTCGCCGCAGCCGCTTGCCGCGGCGCTGGCGCAACAGGCGAGCGAAGGGCTGAGCTATATGCTGCC
TACCGAGCGCGGCGCGACGCTGGCAGAACGGCTCGCGGCGACCTTTGGCCTGCCGCTGTGGCAGGTGACGACCACCGCCA
GCGAGGCCAATCGTGCGGTCATTCGCTGGTGCCGCGGCATCACCGGCCGCGCGAAGATCCTGATCTTCAACGGCGCCTAT
CATGGCGCGGTCGACGACGCCTTTGTCGATCTGACAGCCGGTCGCCCGGCGATGCGGGCAAGTCTGGTCGGACAGGTCCA
TGACCTGACCGCCACAACCGAAGTGGTCGAGTTCAACGACGAAGATGCGCTGGCGTCGGCGTTACGGCGGGGCGACATCG
CCTGCGTGCTTGCCGAGCCGGTGATGACCAATATCGGCATGGTGCGCGATGCGCCCGGCTTTCTCGGGGCGCTTCGCGCA
CTCTGCGACGAAACCGGCACATTGCTGGTGTTCGATGAGACGCATACGGTCTCGTCGGGCCATGGCGGCCACGGCGTCAC
CCATGGACCACGGCCCGACCTGATGGTCATCGGCAAGTCGATCGGCGGCGGTATACCTTGCGCGGTTTATGGCTTCAGCG
CCGCGGTCGGCGATCATATGGTCGCGCTCAACCATACGCGCCCGCCGGGGCACAGCGGGATCGGCACCACCCTGTCGGCC
AACGCGCTCGCGATCACCGCGATGGATACCATGCTGGCGGAGGTCATCACACCGGCCGCCTATGACCAAATGCTACGCGG
CGCGGCGCGGCTCGTCGCCGGGCTGGAGCAGGAGATTGCCCGCGCCGCGGTCGACTGGCACGTCACCCAGGTCGGCGCGC
GCGTCGAGTTTCTCACCTGCCCGACCCCGCCGCGCAATGGCCGCGAAGCCAGGGCGGCGATGCAGCCCGAACTCGAAGCG
GCAATCCACCTCTTCCTGGCGAACCGCGGGATTTTGCTGGCGCCCTTCCACAATATGATGTTGGTGAGTCCGGTGACGGG
CGACGATCAGATCGACCGGCTGGTCGGCGCGTTCGCCGACTGCGTGAGCGCGTTGAAGGAGTAA

Upstream 100 bases:

>100_bases
TCGCGAACCTTCCCTCCCGCCCCTTCCCCGGAGGCAACTGGCTTCAACGCCCGCTCGCGACGCTGGGACTGCTTTTCTAT
GCATTGAAGGACCGGCTCGG

Downstream 100 bases:

>100_bases
GCGGGCGATGTCGAAGTCATCGGGCGTGATTGCGGACCGGTCCGAAGCCGAGGCCTTTTTCAAGGCGAACCCTGACGTCG
ATGCGATCGAGATGATCTAT

Product: aminotransferase

Products: NA

Alternate protein names: GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT [H]

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MIAGAAIARVAAREAERFRTLNPRAFAHHAAAKGWFQAVPFHWMTDWPSPVPIVAASARGATLTSIDGQRYDDFCLGDTA
SLFGHSPQPLAAALAQQASEGLSYMLPTERGATLAERLAATFGLPLWQVTTTASEANRAVIRWCRGITGRAKILIFNGAY
HGAVDDAFVDLTAGRPAMRASLVGQVHDLTATTEVVEFNDEDALASALRRGDIACVLAEPVMTNIGMVRDAPGFLGALRA
LCDETGTLLVFDETHTVSSGHGGHGVTHGPRPDLMVIGKSIGGGIPCAVYGFSAAVGDHMVALNHTRPPGHSGIGTTLSA
NALAITAMDTMLAEVITPAAYDQMLRGAARLVAGLEQEIARAAVDWHVTQVGARVEFLTCPTPPRNGREARAAMQPELEA
AIHLFLANRGILLAPFHNMMLVSPVTGDDQIDRLVGAFADCVSALKE

Sequences:

>Translated_447_residues
MIAGAAIARVAAREAERFRTLNPRAFAHHAAAKGWFQAVPFHWMTDWPSPVPIVAASARGATLTSIDGQRYDDFCLGDTA
SLFGHSPQPLAAALAQQASEGLSYMLPTERGATLAERLAATFGLPLWQVTTTASEANRAVIRWCRGITGRAKILIFNGAY
HGAVDDAFVDLTAGRPAMRASLVGQVHDLTATTEVVEFNDEDALASALRRGDIACVLAEPVMTNIGMVRDAPGFLGALRA
LCDETGTLLVFDETHTVSSGHGGHGVTHGPRPDLMVIGKSIGGGIPCAVYGFSAAVGDHMVALNHTRPPGHSGIGTTLSA
NALAITAMDTMLAEVITPAAYDQMLRGAARLVAGLEQEIARAAVDWHVTQVGARVEFLTCPTPPRNGREARAAMQPELEA
AIHLFLANRGILLAPFHNMMLVSPVTGDDQIDRLVGAFADCVSALKE
>Mature_447_residues
MIAGAAIARVAAREAERFRTLNPRAFAHHAAAKGWFQAVPFHWMTDWPSPVPIVAASARGATLTSIDGQRYDDFCLGDTA
SLFGHSPQPLAAALAQQASEGLSYMLPTERGATLAERLAATFGLPLWQVTTTASEANRAVIRWCRGITGRAKILIFNGAY
HGAVDDAFVDLTAGRPAMRASLVGQVHDLTATTEVVEFNDEDALASALRRGDIACVLAEPVMTNIGMVRDAPGFLGALRA
LCDETGTLLVFDETHTVSSGHGGHGVTHGPRPDLMVIGKSIGGGIPCAVYGFSAAVGDHMVALNHTRPPGHSGIGTTLSA
NALAITAMDTMLAEVITPAAYDQMLRGAARLVAGLEQEIARAAVDWHVTQVGARVEFLTCPTPPRNGREARAAMQPELEA
AIHLFLANRGILLAPFHNMMLVSPVTGDDQIDRLVGAFADCVSALKE

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786349, Length=376, Percent_Identity=32.4468085106383, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1788044, Length=324, Percent_Identity=28.7037037037037, Blast_Score=92, Evalue=8e-20,
Organism=Escherichia coli, GI1789016, Length=427, Percent_Identity=27.6346604215457, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1787560, Length=287, Percent_Identity=28.5714285714286, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1789759, Length=411, Percent_Identity=24.5742092457421, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI145693181, Length=242, Percent_Identity=28.099173553719, Blast_Score=66, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: =5.4.3.8 [H]

Molecular weight: Translated: 47235; Mature: 47235

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAGAAIARVAAREAERFRTLNPRAFAHHAAAKGWFQAVPFHWMTDWPSPVPIVAASARG
CCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEECCCCCCCCEEEECCCC
ATLTSIDGQRYDDFCLGDTASLFGHSPQPLAAALAQQASEGLSYMLPTERGATLAERLAA
CEEEECCCCCCCCCCCCCCHHHCCCCCCHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHH
TFGLPLWQVTTTASEANRAVIRWCRGITGRAKILIFNGAYHGAVDDAFVDLTAGRPAMRA
HHCCCCEEEECCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCEEEECCCCHHHHH
SLVGQVHDLTATTEVVEFNDEDALASALRRGDIACVLAEPVMTNIGMVRDAPGFLGALRA
HHHHHHHHHHHHEEEEEECCHHHHHHHHHCCCEEEEEECHHHHHCCCCCCCCHHHHHHHH
LCDETGTLLVFDETHTVSSGHGGHGVTHGPRPDLMVIGKSIGGGIPCAVYGFSAAVGDHM
HHCCCCEEEEEECCCEECCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCEE
VALNHTRPPGHSGIGTTLSANALAITAMDTMLAEVITPAAYDQMLRGAARLVAGLEQEIA
EEEECCCCCCCCCCCCEECCCEEEEHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHH
RAAVDWHVTQVGARVEFLTCPTPPRNGREARAAMQPELEAAIHLFLANRGILLAPFHNMM
HHHHHHHHEECCCEEEEEECCCCCCCCCHHHHHCCHHHHHHHHHHHHCCCEEEEECCCEE
LVSPVTGDDQIDRLVGAFADCVSALKE
EEECCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIAGAAIARVAAREAERFRTLNPRAFAHHAAAKGWFQAVPFHWMTDWPSPVPIVAASARG
CCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEECCCCCCCCEEEECCCC
ATLTSIDGQRYDDFCLGDTASLFGHSPQPLAAALAQQASEGLSYMLPTERGATLAERLAA
CEEEECCCCCCCCCCCCCCHHHCCCCCCHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHH
TFGLPLWQVTTTASEANRAVIRWCRGITGRAKILIFNGAYHGAVDDAFVDLTAGRPAMRA
HHCCCCEEEECCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCEEEECCCCHHHHH
SLVGQVHDLTATTEVVEFNDEDALASALRRGDIACVLAEPVMTNIGMVRDAPGFLGALRA
HHHHHHHHHHHHEEEEEECCHHHHHHHHHCCCEEEEEECHHHHHCCCCCCCCHHHHHHHH
LCDETGTLLVFDETHTVSSGHGGHGVTHGPRPDLMVIGKSIGGGIPCAVYGFSAAVGDHM
HHCCCCEEEEEECCCEECCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCEE
VALNHTRPPGHSGIGTTLSANALAITAMDTMLAEVITPAAYDQMLRGAARLVAGLEQEIA
EEEECCCCCCCCCCCCEECCCEEEEHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHH
RAAVDWHVTQVGARVEFLTCPTPPRNGREARAAMQPELEAAIHLFLANRGILLAPFHNMM
HHHHHHHHEECCCEEEEEECCCCCCCCCHHHHHCCHHHHHHHHHHHHCCCEEEEECCCEE
LVSPVTGDDQIDRLVGAFADCVSALKE
EEECCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA