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The map label for this gene is potB [H]

Identifier: 103486604

GI number: 103486604

Start: 1151544

End: 1152449

Strand: Reverse

Name: potB [H]

Synonym: Sala_1116

Alternate gene names: 103486604

Gene position: 1152449-1151544 (Counterclockwise)

Preceding gene: 103486605

Following gene: 103486603

Centisome position: 34.45

GC content: 63.02

Gene sequence:

>906_bases
GTGGCCGAGCAGGACTGGAAAACCAACAGGGGCGTTTTCGCGGCGGTGTCGCTGCCGACGCTCCTGTGGGTTCTGCTCTT
CTTCCTCGTCCCCATGGCGATCGTCTGGCTCTACAGCTTTGGCGAAAACCGCGGGCTCACCGAAATCGCCATTTCGGGCA
CGCTCGACAATTACAAGCGCGCCACCGAGTGGCTCTACCTCACCATATTCGGCAAGAGTTTCGCGGTCGCGGCGCTCGTT
ACGCTCATCTGCCTGATCATCGGTTTTCCGGTCGCGATGGCGATCACCTTTGCCAGCGAGAAGTGGCGGCCGTGGCTGCT
CCTCGGCATCATGCTGCCCTTCTGGACCAACCTGCTCATCCGCACCTATGCGCTGATGATGCTGCTCGGCACGCAGGGCT
TCGCCAACAAGGGCCTCGGTGTCCTGTGGGAAGGCGCAAGCTGGATGAAGGCGCTTGTCGGGCTCCAGCCGCTCCCGACG
TGGGAGCCGGTCGAACTGCTCTTCAACAATTTCGCGGTCGTGTTCGGACTCGTCTATGTCCACCTGCCCTTCATGGTGCT
GCCGCTCTATGCCGCGCTCGACCGGCTCGACCGCAGCCTGATCGAGGCGAGCCTCGATCTGGGCGCCGGCCATTTCCGCA
CGATCATGCGGATCGTCGTCCCGCTCGCCGCCCCCGGCATCATCGCCGGGGTGATGATTACCCTGATTCCCGCGCTCGGC
GCCTATCTTACCCCCGACCTGATGGGTGGCACCGACAGCCAGATGATCGCCAATGTGATCGAGCGCCAGTTCAAGAAGGC
GAATGACTGGCCTTTCGGCGCCGCGCTGTCCTTTCTGCTCATCTACGCGATGTTCATCCTCATTGCGCTGCAATCGCTGC
GGCGCCGCGCGCCGGAGGCGCATTGA

Upstream 100 bases:

>100_bases
CAAGTGCGAATATGCACGCTTCAACCCCGAGCTTCAGCCACTCTTCGAAGAGGCGTTCACGCGCGTGCGGGCGGCCTGAC
GCGATCCAGGGACGGCGGGG

Downstream 100 bases:

>100_bases
TGGCGCTCTTCGCCCGCACCCCGCGCGCACCGCTCGAATATAGCCGGACGCTGTGGATGCGCCTGTGGGTCGGCGCGGTG
ATGGTCTTTCTCTACGCGCC

Product: binding-protein-dependent transport systems inner membrane component

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 301; Mature: 300

Protein sequence:

>301_residues
MAEQDWKTNRGVFAAVSLPTLLWVLLFFLVPMAIVWLYSFGENRGLTEIAISGTLDNYKRATEWLYLTIFGKSFAVAALV
TLICLIIGFPVAMAITFASEKWRPWLLLGIMLPFWTNLLIRTYALMMLLGTQGFANKGLGVLWEGASWMKALVGLQPLPT
WEPVELLFNNFAVVFGLVYVHLPFMVLPLYAALDRLDRSLIEASLDLGAGHFRTIMRIVVPLAAPGIIAGVMITLIPALG
AYLTPDLMGGTDSQMIANVIERQFKKANDWPFGAALSFLLIYAMFILIALQSLRRRAPEAH

Sequences:

>Translated_301_residues
MAEQDWKTNRGVFAAVSLPTLLWVLLFFLVPMAIVWLYSFGENRGLTEIAISGTLDNYKRATEWLYLTIFGKSFAVAALV
TLICLIIGFPVAMAITFASEKWRPWLLLGIMLPFWTNLLIRTYALMMLLGTQGFANKGLGVLWEGASWMKALVGLQPLPT
WEPVELLFNNFAVVFGLVYVHLPFMVLPLYAALDRLDRSLIEASLDLGAGHFRTIMRIVVPLAAPGIIAGVMITLIPALG
AYLTPDLMGGTDSQMIANVIERQFKKANDWPFGAALSFLLIYAMFILIALQSLRRRAPEAH
>Mature_300_residues
AEQDWKTNRGVFAAVSLPTLLWVLLFFLVPMAIVWLYSFGENRGLTEIAISGTLDNYKRATEWLYLTIFGKSFAVAALVT
LICLIIGFPVAMAITFASEKWRPWLLLGIMLPFWTNLLIRTYALMMLLGTQGFANKGLGVLWEGASWMKALVGLQPLPTW
EPVELLFNNFAVVFGLVYVHLPFMVLPLYAALDRLDRSLIEASLDLGAGHFRTIMRIVVPLAAPGIIAGVMITLIPALGA
YLTPDLMGGTDSQMIANVIERQFKKANDWPFGAALSFLLIYAMFILIALQSLRRRAPEAH

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=262, Percent_Identity=40.4580152671756, Blast_Score=196, Evalue=1e-51,
Organism=Escherichia coli, GI1787080, Length=242, Percent_Identity=39.6694214876033, Blast_Score=167, Evalue=6e-43,
Organism=Escherichia coli, GI1787713, Length=227, Percent_Identity=32.5991189427313, Blast_Score=98, Evalue=8e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 33513; Mature: 33381

Theoretical pI: Translated: 8.92; Mature: 8.92

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEQDWKTNRGVFAAVSLPTLLWVLLFFLVPMAIVWLYSFGENRGLTEIAISGTLDNYKR
CCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHHHH
ATEWLYLTIFGKSFAVAALVTLICLIIGFPVAMAITFASEKWRPWLLLGIMLPFWTNLLI
HHHEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
RTYALMMLLGTQGFANKGLGVLWEGASWMKALVGLQPLPTWEPVELLFNNFAVVFGLVYV
HHHHHHHHHHCCCCCCCCCCEEECCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHH
HLPFMVLPLYAALDRLDRSLIEASLDLGAGHFRTIMRIVVPLAAPGIIAGVMITLIPALG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
AYLTPDLMGGTDSQMIANVIERQFKKANDWPFGAALSFLLIYAMFILIALQSLRRRAPEA
HHHCHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
H
C
>Mature Secondary Structure 
AEQDWKTNRGVFAAVSLPTLLWVLLFFLVPMAIVWLYSFGENRGLTEIAISGTLDNYKR
CCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHHHH
ATEWLYLTIFGKSFAVAALVTLICLIIGFPVAMAITFASEKWRPWLLLGIMLPFWTNLLI
HHHEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
RTYALMMLLGTQGFANKGLGVLWEGASWMKALVGLQPLPTWEPVELLFNNFAVVFGLVYV
HHHHHHHHHHCCCCCCCCCCEEECCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHH
HLPFMVLPLYAALDRLDRSLIEASLDLGAGHFRTIMRIVVPLAAPGIIAGVMITLIPALG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
AYLTPDLMGGTDSQMIANVIERQFKKANDWPFGAALSFLLIYAMFILIALQSLRRRAPEA
HHHCHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
H
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]