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The map label for this gene is potC [H]

Identifier: 103486603

GI number: 103486603

Start: 1150702

End: 1151547

Strand: Reverse

Name: potC [H]

Synonym: Sala_1115

Alternate gene names: 103486603

Gene position: 1151547-1150702 (Counterclockwise)

Preceding gene: 103486604

Following gene: 103486602

Centisome position: 34.42

GC content: 61.7

Gene sequence:

>846_bases
TTGATGGCGCTCTTCGCCCGCACCCCGCGCGCACCGCTCGAATATAGCCGGACGCTGTGGATGCGCCTGTGGGTCGGCGC
GGTGATGGTCTTTCTCTACGCGCCGCTGGTGGTGCTGATGATCTTCAGCTTCAACGACAGCAAGCGCAACGTCGTATGGC
GCGGCTTCACGACGAAATATTATGAGAAGGCACTGGGCAACGACCAGCTGGTCGAGGCGCTGGTCAATTCGCTGACCATC
GCCGCGCTGGCGACGCTCGCCAGCCTCGTGCTTGGCACGCTCGCCGCGGTGATGCTCTGGCGCTTTCGTTTCCCGTGGAA
GGGCGCGATCGACGGCACCATCTCGTTGCCGATCATCGTTCCCGAAATCTGCCTCGGCGTAGCGTTCCTGATGTTTTTCG
CGGCAATCGGCTGGCCGAACGACCTCGTCTGGCCGTTCAACCTCGGCGCGATCACCATCGCGCACATCACCTTCTGCTTC
CCCTTCGTCACCATGGTCGTGCGCTCGCGCCTCGCAAGCTTCAACCGCGAGCAGGAGGAAGCCGCGAAGGATCTGGGCGC
CAGCGAATGGCAGGTGTTCCGCGACGTGCTGATTCCGCACATGAAACCGGCGCTCGTCGCGGGTGCGTTGCTATCATTCA
CCCTCAGCCTCGACGATTTCGTCATCACCTATTTCACCAGCGGCCCCGACACGATCACTTTCCCGGTGAAGGTCTATTCG
ATGGTCCGCTTCTCGGTGACGCCAGAGGTCAACGCGGCATCGACCCTGCTCATCATCCTTACCGTCGTCCTGACCTTCGT
TGCGCTGAAGGCGCAGGGTGTGAAAGCCATTGCGGAAACCCACTGA

Upstream 100 bases:

>100_bases
ATGACTGGCCTTTCGGCGCCGCGCTGTCCTTTCTGCTCATCTACGCGATGTTCATCCTCATTGCGCTGCAATCGCTGCGG
CGCCGCGCGCCGGAGGCGCA

Downstream 100 bases:

>100_bases
CCATGACCGAACCCGCCCAACCGATCATCCAGATCCAGAACGTCTCGAAACGCTTCGGCAAGGTCACGGCGGTCGACAAT
GTCAGCCTCGACATCAACGC

Product: binding-protein-dependent transport systems inner membrane component

Products: Phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MMALFARTPRAPLEYSRTLWMRLWVGAVMVFLYAPLVVLMIFSFNDSKRNVVWRGFTTKYYEKALGNDQLVEALVNSLTI
AALATLASLVLGTLAAVMLWRFRFPWKGAIDGTISLPIIVPEICLGVAFLMFFAAIGWPNDLVWPFNLGAITIAHITFCF
PFVTMVVRSRLASFNREQEEAAKDLGASEWQVFRDVLIPHMKPALVAGALLSFTLSLDDFVITYFTSGPDTITFPVKVYS
MVRFSVTPEVNAASTLLIILTVVLTFVALKAQGVKAIAETH

Sequences:

>Translated_281_residues
MMALFARTPRAPLEYSRTLWMRLWVGAVMVFLYAPLVVLMIFSFNDSKRNVVWRGFTTKYYEKALGNDQLVEALVNSLTI
AALATLASLVLGTLAAVMLWRFRFPWKGAIDGTISLPIIVPEICLGVAFLMFFAAIGWPNDLVWPFNLGAITIAHITFCF
PFVTMVVRSRLASFNREQEEAAKDLGASEWQVFRDVLIPHMKPALVAGALLSFTLSLDDFVITYFTSGPDTITFPVKVYS
MVRFSVTPEVNAASTLLIILTVVLTFVALKAQGVKAIAETH
>Mature_281_residues
MMALFARTPRAPLEYSRTLWMRLWVGAVMVFLYAPLVVLMIFSFNDSKRNVVWRGFTTKYYEKALGNDQLVEALVNSLTI
AALATLASLVLGTLAAVMLWRFRFPWKGAIDGTISLPIIVPEICLGVAFLMFFAAIGWPNDLVWPFNLGAITIAHITFCF
PFVTMVVRSRLASFNREQEEAAKDLGASEWQVFRDVLIPHMKPALVAGALLSFTLSLDDFVITYFTSGPDTITFPVKVYS
MVRFSVTPEVNAASTLLIILTVVLTFVALKAQGVKAIAETH

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1177

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component II

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787368, Length=249, Percent_Identity=40.1606425702811, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI1787081, Length=245, Percent_Identity=44.4897959183673, Blast_Score=172, Evalue=2e-44,
Organism=Escherichia coli, GI1787714, Length=260, Percent_Identity=34.6153846153846, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1788764, Length=190, Percent_Identity=33.6842105263158, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI87082099, Length=238, Percent_Identity=25.6302521008403, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1786980, Length=208, Percent_Identity=27.8846153846154, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1786254, Length=208, Percent_Identity=26.9230769230769, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1787369, Length=222, Percent_Identity=26.5765765765766, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 31254; Mature: 31254

Theoretical pI: Translated: 9.07; Mature: 9.07

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMALFARTPRAPLEYSRTLWMRLWVGAVMVFLYAPLVVLMIFSFNDSKRNVVWRGFTTKY
CCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHH
YEKALGNDQLVEALVNSLTIAALATLASLVLGTLAAVMLWRFRFPWKGAIDGTISLPIIV
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECHHHH
PEICLGVAFLMFFAAIGWPNDLVWPFNLGAITIAHITFCFPFVTMVVRSRLASFNREQEE
HHHHHHHHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
AAKDLGASEWQVFRDVLIPHMKPALVAGALLSFTLSLDDFVITYFTSGPDTITFPVKVYS
HHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHEEEEEEECCCCEEEEHHHHHH
MVRFSVTPEVNAASTLLIILTVVLTFVALKAQGVKAIAETH
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHCCC
>Mature Secondary Structure
MMALFARTPRAPLEYSRTLWMRLWVGAVMVFLYAPLVVLMIFSFNDSKRNVVWRGFTTKY
CCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHH
YEKALGNDQLVEALVNSLTIAALATLASLVLGTLAAVMLWRFRFPWKGAIDGTISLPIIV
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECHHHH
PEICLGVAFLMFFAAIGWPNDLVWPFNLGAITIAHITFCFPFVTMVVRSRLASFNREQEE
HHHHHHHHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
AAKDLGASEWQVFRDVLIPHMKPALVAGALLSFTLSLDDFVITYFTSGPDTITFPVKVYS
HHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHEEEEEEECCCCEEEEHHHHHH
MVRFSVTPEVNAASTLLIILTVVLTFVALKAQGVKAIAETH
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]