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The map label for this gene is metZ [H]

Identifier: 103486575

GI number: 103486575

Start: 1115155

End: 1116360

Strand: Direct

Name: metZ [H]

Synonym: Sala_1086

Alternate gene names: 103486575

Gene position: 1115155-1116360 (Clockwise)

Preceding gene: 103486574

Following gene: 103486576

Centisome position: 33.34

GC content: 63.27

Gene sequence:

>1206_bases
ATGAAAAAGACGACGGGTCTCGACCGGAACAAGACGAAGACGTGGCATCCCGCGACGCAGGCGGTGCGCGGCGGCACGTG
GCGCAGCGAGCATGGCGAGACGTCGGAGGCGCTCTTCCTGACCTCGGGCTATACCTATGACAGTGCCGAGGAAGTGGCCG
CCCGCTTTGCCGGCGAAGCGCAGGGCATGACCTATTCGCGGCTCCAGAATCCGACCGTGGCGATGCTGGAGGAACGCATC
GCGCTGATGGAGGGGGCCGAGGCGTGCCGCACGCAGGCGACGGGCATGGCGGCGATGACCACCGCGCTGCTCTGCCAATT
GTCGGCGGGCGACCATATCGTCGCGGCGAAGGCGGCGTTTGGCTCGTGCCGCTGGTTGATCGACCATCTCTGTCCGCGCT
TCGGGATCGACACCACCGTCGTCGATGGCCGCGACAATGAGGCGTGGGAACGGGCCATCCAGACGAATACTAGGGTCTTT
TTCTTTGAAACCCCGGCCAATCCGACGATGGATATTGTCGACATGCGGCATGTGTGCGCGCTGGCGAAGGCGCATGGAAT
CACCAGTGTCGTCGATAATGCCTTTGCGACGAGCGCGCTCCAGCGCCCGATGGATTTCGGCGCCGATGTCGTGGCCTATT
CGGCGACCAAGCTGATGGAGGGGCAGGGGCGCGTGCTCGCGGGCGCGGTGTGTGGGACCGAAAAGTTCATCAACGAGGTG
CTGCTGCCGTTTCAGCGCAACACGGGGCCGAACCTGTCGCCCTTCAACGCCTGGGTGGTGATGAAGGGACTGGAGACGCT
CGACCTGCGCGCGCGGCGCCAGTCGGAAAACGCGCTGGCCGTCGGCAAGTTTGTCGAAAGCCGGGTGCCGCGCATCCTGC
ATCCCGGCCTTGCGAGCCATCCGCAGCATGATCTGGCGAAAAGCCAGATGGAGGCTTGCGGACCGATCTTTTCCTTCATT
CTCGACGGGCGCGAACAGGCGATGGCATTTCTCAACGCGCTCGAACTGGTCGACATTTCCAACAATATCGGCGATTCGCG
CAGCCTTTGTTGTCACCCCGCATCTACCACCCATTACAGTGTCAGCGCCGAGGCGCGCGCCGATATGGGCGTCGTGGAAG
GAATGCTGCGTATCAACATCGGGCTCGAAGATCCGCGCGACATCATCGCCGATCTGGACCAGGCATTGACCAGGGTCGGG
CTTTAG

Upstream 100 bases:

>100_bases
AGATACTCGCCGACGGCTGCCGCGGCGCCGACCTGGGCGGAACGATGGGCACGGCGGCCTTGGGCGATGCGGTCGTGGCG
GCTTTGAACGGATAGGGCAG

Downstream 100 bases:

>100_bases
GGGAATCTGGCGCCGCGATGATCGACTCCTTCTTTCCCTTCTCGGCGACCACCGGACCCGTGACGGTGCGCGTGGCGGTG
AGCTATCTGCCCGAACAGTC

Product: cystathionine gamma-synthase

Products: methanethiol; NH3; 2-oxobutanoate

Alternate protein names: OSH sulfhydrylase [H]

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MKKTTGLDRNKTKTWHPATQAVRGGTWRSEHGETSEALFLTSGYTYDSAEEVAARFAGEAQGMTYSRLQNPTVAMLEERI
ALMEGAEACRTQATGMAAMTTALLCQLSAGDHIVAAKAAFGSCRWLIDHLCPRFGIDTTVVDGRDNEAWERAIQTNTRVF
FFETPANPTMDIVDMRHVCALAKAHGITSVVDNAFATSALQRPMDFGADVVAYSATKLMEGQGRVLAGAVCGTEKFINEV
LLPFQRNTGPNLSPFNAWVVMKGLETLDLRARRQSENALAVGKFVESRVPRILHPGLASHPQHDLAKSQMEACGPIFSFI
LDGREQAMAFLNALELVDISNNIGDSRSLCCHPASTTHYSVSAEARADMGVVEGMLRINIGLEDPRDIIADLDQALTRVG
L

Sequences:

>Translated_401_residues
MKKTTGLDRNKTKTWHPATQAVRGGTWRSEHGETSEALFLTSGYTYDSAEEVAARFAGEAQGMTYSRLQNPTVAMLEERI
ALMEGAEACRTQATGMAAMTTALLCQLSAGDHIVAAKAAFGSCRWLIDHLCPRFGIDTTVVDGRDNEAWERAIQTNTRVF
FFETPANPTMDIVDMRHVCALAKAHGITSVVDNAFATSALQRPMDFGADVVAYSATKLMEGQGRVLAGAVCGTEKFINEV
LLPFQRNTGPNLSPFNAWVVMKGLETLDLRARRQSENALAVGKFVESRVPRILHPGLASHPQHDLAKSQMEACGPIFSFI
LDGREQAMAFLNALELVDISNNIGDSRSLCCHPASTTHYSVSAEARADMGVVEGMLRINIGLEDPRDIIADLDQALTRVG
L
>Mature_401_residues
MKKTTGLDRNKTKTWHPATQAVRGGTWRSEHGETSEALFLTSGYTYDSAEEVAARFAGEAQGMTYSRLQNPTVAMLEERI
ALMEGAEACRTQATGMAAMTTALLCQLSAGDHIVAAKAAFGSCRWLIDHLCPRFGIDTTVVDGRDNEAWERAIQTNTRVF
FFETPANPTMDIVDMRHVCALAKAHGITSVVDNAFATSALQRPMDFGADVVAYSATKLMEGQGRVLAGAVCGTEKFINEV
LLPFQRNTGPNLSPFNAWVVMKGLETLDLRARRQSENALAVGKFVESRVPRILHPGLASHPQHDLAKSQMEACGPIFSFI
LDGREQAMAFLNALELVDISNNIGDSRSLCCHPASTTHYSVSAEARADMGVVEGMLRINIGLEDPRDIIADLDQALTRVG
L

Specific function: Converts O-succinylhomoserine into homocysteine [H]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=328, Percent_Identity=34.7560975609756, Blast_Score=200, Evalue=2e-51,
Organism=Homo sapiens, GI299473759, Length=343, Percent_Identity=33.5276967930029, Blast_Score=171, Evalue=8e-43,
Organism=Homo sapiens, GI25453487, Length=342, Percent_Identity=31.5789473684211, Blast_Score=149, Evalue=4e-36,
Organism=Escherichia coli, GI1790375, Length=387, Percent_Identity=33.5917312661499, Blast_Score=208, Evalue=6e-55,
Organism=Escherichia coli, GI1789383, Length=391, Percent_Identity=25.5754475703325, Blast_Score=132, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI17538055, Length=363, Percent_Identity=38.0165289256198, Blast_Score=229, Evalue=2e-60,
Organism=Caenorhabditis elegans, GI17560056, Length=345, Percent_Identity=37.1014492753623, Blast_Score=217, Evalue=1e-56,
Organism=Caenorhabditis elegans, GI71980708, Length=369, Percent_Identity=31.9783197831978, Blast_Score=196, Evalue=2e-50,
Organism=Saccharomyces cerevisiae, GI6319307, Length=391, Percent_Identity=31.2020460358056, Blast_Score=194, Evalue=3e-50,
Organism=Saccharomyces cerevisiae, GI6323333, Length=410, Percent_Identity=31.219512195122, Blast_Score=194, Evalue=3e-50,
Organism=Saccharomyces cerevisiae, GI6321254, Length=404, Percent_Identity=25.4950495049505, Blast_Score=124, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6321170, Length=290, Percent_Identity=26.551724137931, Blast_Score=121, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6321904, Length=341, Percent_Identity=25.5131964809384, Blast_Score=102, Evalue=1e-22,
Organism=Drosophila melanogaster, GI19922554, Length=346, Percent_Identity=37.8612716763006, Blast_Score=236, Evalue=2e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006234
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: 4.4.1.11

Molecular weight: Translated: 43621; Mature: 43621

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTTGLDRNKTKTWHPATQAVRGGTWRSEHGETSEALFLTSGYTYDSAEEVAARFAGEA
CCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHCCCC
QGMTYSRLQNPTVAMLEERIALMEGAEACRTQATGMAAMTTALLCQLSAGDHIVAAKAAF
CCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCEEEEEHHHH
GSCRWLIDHLCPRFGIDTTVVDGRDNEAWERAIQTNTRVFFFETPANPTMDIVDMRHVCA
HHHHHHHHHHCHHCCCCCEEECCCCCHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHH
LAKAHGITSVVDNAFATSALQRPMDFGADVVAYSATKLMEGQGRVLAGAVCGTEKFINEV
HHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEEHHCHHHHHHHH
LLPFQRNTGPNLSPFNAWVVMKGLETLDLRARRQSENALAVGKFVESRVPRILHPGLASH
HCCCCCCCCCCCCCCHHEEEECCCHHHHHHHHHCCCCHHHHHHHHHHCCCHHHCCCCCCC
PQHDLAKSQMEACGPIFSFILDGREQAMAFLNALELVDISNNIGDSRSLCCHPASTTHYS
CHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCEEE
VSAEARADMGVVEGMLRINIGLEDPRDIIADLDQALTRVGL
EECHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKTTGLDRNKTKTWHPATQAVRGGTWRSEHGETSEALFLTSGYTYDSAEEVAARFAGEA
CCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHCCCC
QGMTYSRLQNPTVAMLEERIALMEGAEACRTQATGMAAMTTALLCQLSAGDHIVAAKAAF
CCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCEEEEEHHHH
GSCRWLIDHLCPRFGIDTTVVDGRDNEAWERAIQTNTRVFFFETPANPTMDIVDMRHVCA
HHHHHHHHHHCHHCCCCCEEECCCCCHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHH
LAKAHGITSVVDNAFATSALQRPMDFGADVVAYSATKLMEGQGRVLAGAVCGTEKFINEV
HHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEEHHCHHHHHHHH
LLPFQRNTGPNLSPFNAWVVMKGLETLDLRARRQSENALAVGKFVESRVPRILHPGLASH
HCCCCCCCCCCCCCCHHEEEECCCHHHHHHHHHCCCCHHHHHHHHHHCCCHHHCCCCCCC
PQHDLAKSQMEACGPIFSFILDGREQAMAFLNALELVDISNNIGDSRSLCCHPASTTHYS
CHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCEEE
VSAEARADMGVVEGMLRINIGLEDPRDIIADLDQALTRVGL
EECHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-methionine

Specific reaction: L-methionine = methanethiol + NH3 + 2-oxobutanoate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704274; 10984043 [H]